Rw1G025370

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018517
Physical Location & Seq
Forward (+)
51996493 .. 51998900
2408 bp
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UTR
Exon/CDS
Intron
Rw1G025370.1

Sequence Viewer

Length: 654 bp
ATGGGTAGCTGCAACTCAAAGAAAGCTGGCCCAAGTGCCAAAGTACCATCTGGGTTGGAACTTAGTCCAACTGTTCTTGCTTCTAAAACTCCCTTTTCTGTGAGTGAGGTGGAGTCTTTGTATGAGCTATACAAGAAATTAAGCAGCTCTGTAGTTAAAGATGGGCTTATACACAAGGAAGAGCTCCAGCTTGCGCTTTTTCAGAGCAGCAGTAAGCAGAACCTCTTTTTCGACAGGATATTTGATCTTTTCGATGTCAATAAAAATGAGCATATTGAGTTTGAAGAGTTTGTTCATACATTGAGTGTTTTCCACCCTAAAACTCCTGATGAAGTCAAAATTACATATGCTTTTAAATTATATGATTTGAGGCACACTGGTTACATCGAGCGTGAGGAGTTGAAGGAGATGGTGCTGGCTCTTCTGAACGAATCTGATCTGATTCTTTCAGATGATATTGTTGAAATGATTGTGAATAAGACATTCATGGAAGCAGATGGAAAAGCAGACGGTAGGATTGATGAAGAAGAGTGGAAGGAATATGTAGCTAAAAATCCTTCTCTTCTGAAAAACATGACTCTTCCATACTTAATGGACATATCTCTAGCATTTCCCAGCTTTGTGCTGAATACTCAAGCAACTAGACATAGTTAA

Protein Analysis

217

Amino Acids

24.72

Weight (kDa)

5.05

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 112 - 181 4e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018517)

Species Orthologous Gene IDs
prunus_persica Prupe.2G188700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359511 RchiOBHm_Chr1g0359521
rosa_multiflora Rmu_sc0002556.1_g000015
rosa_roxburghii Rroxscaffold_4G00296920
rosa_rugosa Rorug01G0272000
rosa_samantha Rh1BG251400 Rh1DG280500
rosa_wichuraiana Rw1G025360 Rw1G025370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 45
AgsI TTSAA 3 cut(s) 284, 403, 464
AluBI AGCT 8 cut(s) 9, 26, 127, 147, 184, 190, 548, 618
AluI AGCT 8 cut(s) 9, 26, 127, 147, 184, 190, 548, 618
Alw21I GWGCWC 1 cut(s) 186
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 3 cut(s) 9, 144, 207
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 156, 219
BccI CCATC 4 cut(s) 55, 155, 403, 491
BfaI CTAG 2 cut(s) 605, 642
BfmI CTRYAG 1 cut(s) 150
BisI GCNGC 3 cut(s) 10, 145, 208
BlsI GCNGC 3 cut(s) 11, 146, 209
BmgT120I GGNCC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 618
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bse1I ACTGG 1 cut(s) 382
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 1 cut(s) 410
BseXI GCAGC 2 cut(s) 156, 219
BseYI CCCAGC 1 cut(s) 614
BshFI GGCC 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 186
BsnI GGCC 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 244, 436
BspANI GGCC 1 cut(s) 30
BspQI GCTCTTC 2 cut(s) 174, 426
BsrI ACTGG 1 cut(s) 382
BssMI GATC 2 cut(s) 244, 436
Bst4CI ACNGT 2 cut(s) 73, 512
Bst6I CTCTTC 6 cut(s) 174, 279, 426, 522, 567, 585
BstC8I GCNNGC 3 cut(s) 28, 192, 417
BstDEI CTNAG 1 cut(s) 62
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 2 cut(s) 247, 439
BstMBI GATC 2 cut(s) 244, 436
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 156, 219
BsuRI GGCC 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 28, 192, 417
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 44
CviAII CATG 2 cut(s) 487, 574
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 1 cut(s) 62
DpnI GATC 2 cut(s) 246, 438
DpnII GATC 2 cut(s) 244, 436
DraI TTTAAA 1 cut(s) 355
Eam1104I CTCTTC 6 cut(s) 174, 279, 426, 522, 567, 585
EarI CTCTTC 6 cut(s) 174, 279, 426, 522, 567, 585
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 2 cut(s) 490, 577
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FatI CATG 2 cut(s) 486, 573
FauNDI CATATG 1 cut(s) 346
Fnu4HI GCNGC 3 cut(s) 10, 145, 208
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 10, 145, 208
FspBI CTAG 2 cut(s) 605, 642
GlaI GCGC 1 cut(s) 195
GluI GCNGC 3 cut(s) 10, 145, 208
GsaI CCCAGC 1 cut(s) 618
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 1 cut(s) 30
HhaI GCGC 1 cut(s) 196
Hin1II CATG 2 cut(s) 490, 577
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HinfI GANTC 4 cut(s) 113, 431, 442, 577
Hpy188I TCNGA 6 cut(s) 204, 426, 436, 441, 451, 567
Hpy188III TCNNGA 1 cut(s) 326
HpyAV CCTTC 3 cut(s) 397, 529, 567
HpyCH4III ACNGT 2 cut(s) 73, 512
HpyCH4V TGCA 1 cut(s) 12
HpyF3I CTNAG 1 cut(s) 62
Hsp92II CATG 2 cut(s) 490, 577
HspAI GCGC 1 cut(s) 194
Kzo9I GATC 2 cut(s) 244, 436
LguI GCTCTTC 2 cut(s) 174, 426
LmnI GCTCC 1 cut(s) 189
LpnPI CCDG 8 cut(s) 12, 36, 200, 220, 339, 363, 401, 628
Lsp1109I GCAGC 2 cut(s) 156, 219
MaeI CTAG 2 cut(s) 605, 642
MaeIII GTNAC 1 cut(s) 380
MalI GATC 2 cut(s) 246, 438
MboI GATC 2 cut(s) 244, 436
MboII GAAGA 7 cut(s) 191, 296, 413, 536, 539, 554, 572
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 3 cut(s) 137, 339, 356
MlyI GAGTC 2 cut(s) 122, 571
MmeI TCCRAC 2 cut(s) 36, 92
MnlI CCTC 4 cut(s) 100, 233, 363, 388
MseI TTAA 5 cut(s) 140, 156, 354, 590, 652
NdeI CATATG 1 cut(s) 346
NdeII GATC 2 cut(s) 244, 436
NlaIII CATG 2 cut(s) 490, 577
PciSI GCTCTTC 2 cut(s) 174, 426
PfeI GAWTC 2 cut(s) 431, 442
PkrI GCNGC 3 cut(s) 11, 146, 209
PleI GAGTC 2 cut(s) 121, 571
PpsI GAGTC 2 cut(s) 121, 571
Psp124BI GAGCTC 1 cut(s) 186
PspFI CCCAGC 1 cut(s) 614
PspPI GGNCC 1 cut(s) 29
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SacI GAGCTC 1 cut(s) 186
SapI GCTCTTC 2 cut(s) 174, 426
SaqAI TTAA 5 cut(s) 140, 156, 354, 590, 652
SatI GCNGC 3 cut(s) 10, 145, 208
Sau3AI GATC 2 cut(s) 244, 436
Sau96I GGNCC 1 cut(s) 29
SchI GAGTC 2 cut(s) 122, 571
SduI GDGCHC 1 cut(s) 186
SfcI CTRYAG 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 633
SmoI CTYRAG 1 cut(s) 633
Sse9I AATT 3 cut(s) 137, 339, 356
SspMI CTAG 2 cut(s) 605, 642
SstI GAGCTC 1 cut(s) 186
TaaI ACNGT 2 cut(s) 73, 512
TaqI TCGA 3 cut(s) 231, 252, 387
TasI AATT 3 cut(s) 137, 339, 356
TfiI GAWTC 2 cut(s) 431, 442
Tru1I TTAA 5 cut(s) 140, 156, 354, 590, 652
Tru9I TTAA 5 cut(s) 140, 156, 354, 590, 652
TscAI CASTG 1 cut(s) 382
TseI GCWGC 3 cut(s) 9, 144, 207
TspDTI ATGAA 4 cut(s) 284, 345, 475, 537
TspRI CASTG 1 cut(s) 382
XspI CTAG 2 cut(s) 605, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.