RH1DG280500

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0018517
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG280500.1

Sequence Viewer

Length: 642 bp
ATGGGTTGCGCTAGCTCAAACAAAGCCAGAGTCAAAACTGGCCCAAGTGCCAATTTAGAGCTTAATCCGGCTATTCTTGCTTTTTCTGTGAGTGAGGTAGAGTCTTTGTATGAGCTATATAAGAAACTAAGCAGCTCTGTAGTTCAAGATGGGCTTATACACAAGGAAGAGTTGCAGCTTGCGCTTTTTCAAAACAACAGTAAGCAGAACCTCTTTTTCGACAGGATATTTGATCTTTTCGATATCAATAAAAATGAGCATATCGAGTTTGAAGAGTTTGTTCATACATTGAGTGTTTTCCACCCTAAAACTCCTGATGAAGTCAAAATTACATATGCTTTTAAATTATATGATTTGAGGCACACTGGTTACATCGAGCGTGAGGAGTTGAAGGAGATGGTGCTGGCTCTTCTGAACGAATCTGATCTGATTCTTTCAGATGATACTGTTGAAATGATTGTGAATAAGACATTCATGGAAGCAGATGGAAAAGCAGATGGTAAGATTGATGAAGAAGAGTGGAAGGAATATGTAGCTAAAAATCCTTCTCTTCTGAAAAACATGACTCTTCCATACTTAATGGACATATCTCTAGCATTTCCCAGCTTTGTGCTGAATACTCAAGCAACTAGACATAATTAA

Protein Analysis

213

Amino Acids

24.45

Weight (kDa)

4.9

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 108 - 177 2.5e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018517)

Species Orthologous Gene IDs
prunus_persica Prupe.2G188700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359511 RchiOBHm_Chr1g0359521
rosa_multiflora Rmu_sc0002556.1_g000015
rosa_roxburghii Rroxscaffold_4G00296920
rosa_rugosa Rorug01G0272000
rosa_samantha Rh1BG251400 Rh1DG280500
rosa_wichuraiana Rw1G025360 Rw1G025370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 5 cut(s) 146, 191, 272, 391, 452
AluBI AGCT 7 cut(s) 15, 61, 115, 135, 178, 536, 606
AluI AGCT 7 cut(s) 15, 61, 115, 135, 178, 536, 606
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 2 cut(s) 132, 175
ArsI GACNNNNNNTTYG 4 cut(s) 15, 47, 212, 244
AspLEI GCGC 2 cut(s) 11, 184
AspS9I GGNCC 1 cut(s) 41
AsuNHI GCTAGC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 144, 187
BccI CCATC 4 cut(s) 143, 391, 479, 491
BfaI CTAG 3 cut(s) 12, 593, 630
BfmI CTRYAG 1 cut(s) 138
BisI GCNGC 2 cut(s) 133, 176
BlsI GCNGC 2 cut(s) 134, 177
BmgT120I GGNCC 1 cut(s) 41
BmtI GCTAGC 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 606
Bse1I ACTGG 2 cut(s) 43, 370
BseNI ACTGG 2 cut(s) 43, 370
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 2 cut(s) 144, 187
BseYI CCCAGC 1 cut(s) 602
BshFI GGCC 1 cut(s) 42
BsiSI CCGG 1 cut(s) 68
BsnI GGCC 1 cut(s) 42
Bsp143I GATC 2 cut(s) 232, 424
BspANI GGCC 1 cut(s) 42
BspOI GCTAGC 1 cut(s) 15
BspQI GCTCTTC 1 cut(s) 414
BsrI ACTGG 2 cut(s) 43, 370
BssMI GATC 2 cut(s) 232, 424
Bst4CI ACNGT 2 cut(s) 200, 448
Bst6I CTCTTC 6 cut(s) 162, 267, 414, 510, 555, 573
BstC8I GCNNGC 3 cut(s) 13, 180, 405
BstDEI CTNAG 1 cut(s) 128
BstHHI GCGC 2 cut(s) 11, 184
BstKTI GATC 2 cut(s) 235, 427
BstMBI GATC 2 cut(s) 232, 424
BstMWI GCNNNNNNNGC 2 cut(s) 77, 181
BstSFI CTRYAG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 144, 187
BsuRI GGCC 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 363
Cac8I GCNNGC 3 cut(s) 13, 180, 405
CfoI GCGC 2 cut(s) 11, 184
Cfr13I GGNCC 1 cut(s) 41
CviAII CATG 2 cut(s) 475, 562
DdeI CTNAG 1 cut(s) 128
DpnI GATC 2 cut(s) 234, 426
DpnII GATC 2 cut(s) 232, 424
DraI TTTAAA 1 cut(s) 343
Eam1104I CTCTTC 6 cut(s) 162, 267, 414, 510, 555, 573
EarI CTCTTC 6 cut(s) 162, 267, 414, 510, 555, 573
Eco32I GATATC 1 cut(s) 244
EcoRV GATATC 1 cut(s) 244
FaeI CATG 2 cut(s) 478, 565
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FatI CATG 2 cut(s) 474, 561
FauNDI CATATG 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 133, 176
Fsp4HI GCNGC 2 cut(s) 133, 176
FspBI CTAG 3 cut(s) 12, 593, 630
GlaI GCGC 2 cut(s) 10, 183
GluI GCNGC 2 cut(s) 133, 176
GsaI CCCAGC 1 cut(s) 606
HaeIII GGCC 1 cut(s) 42
HapII CCGG 1 cut(s) 68
HhaI GCGC 2 cut(s) 11, 184
Hin1II CATG 2 cut(s) 478, 565
Hin6I GCGC 2 cut(s) 9, 182
HinP1I GCGC 2 cut(s) 9, 182
HinfI GANTC 5 cut(s) 30, 101, 419, 430, 565
HpaII CCGG 1 cut(s) 68
Hpy188I TCNGA 5 cut(s) 414, 424, 429, 439, 555
Hpy188III TCNNGA 2 cut(s) 146, 314
HpyAV CCTTC 3 cut(s) 385, 517, 555
HpyCH4III ACNGT 2 cut(s) 200, 448
HpyCH4V TGCA 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 181
HpyF3I CTNAG 1 cut(s) 128
Hsp92II CATG 2 cut(s) 478, 565
HspAI GCGC 2 cut(s) 9, 182
Kzo9I GATC 2 cut(s) 232, 424
LguI GCTCTTC 1 cut(s) 414
LpnPI CCDG 8 cut(s) 24, 40, 81, 208, 327, 351, 389, 616
Lsp1109I GCAGC 2 cut(s) 144, 187
MaeI CTAG 3 cut(s) 12, 593, 630
MaeIII GTNAC 1 cut(s) 368
MalI GATC 2 cut(s) 234, 426
MboI GATC 2 cut(s) 232, 424
MboII GAAGA 7 cut(s) 179, 284, 401, 524, 527, 542, 560
MluCI AATT 4 cut(s) 52, 327, 344, 637
MlyI GAGTC 3 cut(s) 39, 110, 559
MnlI CCTC 4 cut(s) 88, 221, 351, 376
MseI TTAA 4 cut(s) 63, 342, 578, 640
MspI CCGG 1 cut(s) 68
MwoI GCNNNNNNNGC 2 cut(s) 77, 181
NdeI CATATG 1 cut(s) 334
NdeII GATC 2 cut(s) 232, 424
NheI GCTAGC 1 cut(s) 11
NlaIII CATG 2 cut(s) 478, 565
PciSI GCTCTTC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 419, 430
PkrI GCNGC 2 cut(s) 134, 177
PleI GAGTC 3 cut(s) 38, 109, 559
PpsI GAGTC 3 cut(s) 38, 109, 559
PspFI CCCAGC 1 cut(s) 602
PspPI GGNCC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 4 cut(s) 63, 342, 578, 640
SatI GCNGC 2 cut(s) 133, 176
Sau3AI GATC 2 cut(s) 232, 424
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 3 cut(s) 39, 110, 559
SetI ASST 9 cut(s) 17, 63, 99, 117, 137, 180, 213, 538, 608
SfcI CTRYAG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
Sse9I AATT 4 cut(s) 52, 327, 344, 637
SspMI CTAG 3 cut(s) 12, 593, 630
TaaI ACNGT 2 cut(s) 200, 448
TaqI TCGA 4 cut(s) 219, 240, 264, 375
TasI AATT 4 cut(s) 52, 327, 344, 637
TfiI GAWTC 2 cut(s) 419, 430
Tru1I TTAA 4 cut(s) 63, 342, 578, 640
Tru9I TTAA 4 cut(s) 63, 342, 578, 640
TscAI CASTG 1 cut(s) 370
TseI GCWGC 2 cut(s) 132, 175
TspDTI ATGAA 4 cut(s) 272, 333, 463, 525
TspRI CASTG 1 cut(s) 370
XspI CTAG 3 cut(s) 12, 593, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.