RchiOBHm_Chr1g0365211

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010764
Physical Location & Seq
Reverse (-)
56176466 .. 56180272
3807 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1743 bp
ATGTCTTCCCCGATCGAACAAGGTGAGAGAGATATAGAGAAGGGTTTGATGACGACTCCAGCGGACCCATCACCTACGCCGTCGCCGTCATCGACGGCATCGGCGCCGGCGCTGGTGCTGTCGAACTCCGGGAAGAGGATGGACCAGGCGGGGAAGAAGAAGTATGTGAAGCAGGTGACCGGGCGGCACAACGACACCGAGCTCCACCTGGCGGCGCAGAAGGGCGATCTGGCAGCCGTGAAGCAGATTCTGCGTGATATTGTTTCGCAGGTGGAGGGGACGGTGAGCGGCGCTGAGTTGGATTCGGAGGTGGCGGAGGTGAGGGCGGCGGTGGTGAATGATGTGAATGAGCTGGGGGAGACGGCGTTGTTCACGGCGGCGGATAGAGGGCATCTGGATGTGGTGAAGGAGTTGTTGAAGTATTCGAATAAGGAGAGTTTTGTGAAGAAGAATCGGTCCGGGTATAGTCCCTTGCATATTGCTGCAACTCATGGCCACCATGCCATTGTTCAGGTACTCCTGGATCAAGACCCCGGGCTAAGCAGGACAACGGGCCCGTCGAATGCTACCCCACTTATATCTGCAGCTCAAAGAGGGCATATAGCTGTGGTTGATGAACTGTTGTCAAAGGATTCTACCTTGTTGGAGATTTCAAGATCAAATGGGAAGAATGCATTACATCTGGCTGCAAGACCAGGGCATGTGGAGATTGTAAGAGCATTGCTAAATAGGGATCCACAATTGGCACGAAGGACTGACAAGAAAGGGCAAACTGCCTTGCATATGGCTGTTAAAGGGACAAGCTGTGAGGTGGTGAAATTGCTTCTTGATGCAGATGCTGCAATTGTGATGCTTCCTGACAAATTTGGTAACACAGCATTACATGTAGCCACCAGGAAGAAGCGAGCGGAGATAGTGAACGAGTTGTTATCCCTCCCAGACAGCAATGTTAATGCACTGAATAGGGACCACAAAACCGCTCTGGACATTGCTGAAGAGCTTCCACTTTCAGAAGAATCAGCAGATGTAAAGGCTTGCCTTTACAAATACGGTGCTGTTAGAGCTAACGAACTGAACCAACCAAGGGATGAACTGAGAAAAACAGTGACTCAGATCAAGAATGATGTTCATATCCAGCTTGAACAAACAAAGAAAACCAATAAAAATGTTCAGAATATTTCGAAAGACCTCAGGAAGCTCCACAGGGAAGGAATCAACAATGCCACCAACTCAGTCACAGTGGTGGCTGTTCTATTTGCAACAGTTGCTTTTGCAGCTATATTTACTGTGCCCGGCGGTGATAACAATGATGGGACGGCTGTGGTAGTGCACTCTTCTCCATTCAAAATCTTCTTCATTTTTAATGCCATTGCTCTTTTTACATCTCTGGCCGTTGTGGTGGTTCAAATTACATTGGTCAGAGGTGAGACAAAAGCAGAGAAAAAGGTCGTAGAAATTATCAATAAGTTGATGTGGCTCGCGTCAGTGTGCACTTCAGTGGCATTCATGGCCTCCTCTTACATTGTGGTTGGCCGGCATCATAAGTGGGCTGCAATTCTGGTAACAGTTGTTGGGGGAATGATAATGGCAGTGGTTCTTGGCACCATGACATATTATGTGGTGAAGTCAAAGCGGCACCGGGCCTTGAGGAAGAAGGATAAGAGCGCAAGGAGGAGTGGATCCAACTCGTGGATGCCCTCAGACTACTCCAATTCAGAAATTGATCGAATCTATGCCCTTTGA

Protein Analysis

580

Amino Acids

63.04

Weight (kDa)

9.36

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 68 - 176 3.7e-16 Ankyrin repeats (3 copies)
Ank_KRIT1 PF24521 119 - 211 9.4e-07 KRIT1 ankyrin-repeats domain
Ank_4 PF13637 123 - 174 4.8e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 187 - 245 1.6e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 192 - 280 1.2e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 226 - 276 1.1e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 251 - 318 1.9e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 270 - 331 6.5e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 275 - 331 4.7e-07 Ankyrin repeats (many copies)
PGG PF13962 403 - 509 6.5e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 163, 259
Acc36I ACCTGC 2 cut(s) 163, 259
AccB1I GGYRCC 3 cut(s) 103, 1601, 1635
AccB7I CCANNNNNTGG 1 cut(s) 1689
AccBSI CCGCTC 3 cut(s) 288, 908, 980
AccII CGCG 1 cut(s) 1481
AclWI GGATC 5 cut(s) 531, 728, 741, 1674, 1687
AcoI YGGCCR 3 cut(s) 493, 1389, 1531
AcsI RAATTY 1 cut(s) 863
AcuI CTGAAG 2 cut(s) 1014, 1479
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 1 cut(s) 516
AfiI CCNNNNNNNGG 4 cut(s) 135, 211, 1084, 1689
AflIII ACRYGT 1 cut(s) 883
AgsI TTSAA 5 cut(s) 418, 654, 1142, 1345, 1406
AjnI CCWGG 5 cut(s) 144, 207, 519, 694, 893
AleI CACNNNNGTG 2 cut(s) 1241, 1496
Alw21I GWGCWC 3 cut(s) 204, 1332, 1493
Alw26I GTCTC 2 cut(s) 353, 1421
Alw44I GTGCAC 2 cut(s) 1328, 1489
AlwI GGATC 5 cut(s) 531, 728, 741, 1674, 1687
AlwNI CAGNNNCTG 2 cut(s) 250, 839
Ama87I CYCGRG 1 cut(s) 533
AoxI GGCC 6 cut(s) 493, 553, 1389, 1509, 1531, 1641
ApaI GGGCCC 1 cut(s) 557
ApaLI GTGCAC 2 cut(s) 1328, 1489
ApeKI GCWGC 7 cut(s) 233, 482, 584, 686, 839, 1274, 1550
ApoI RAATTY 1 cut(s) 863
Asp700I GAANNNNTTC 1 cut(s) 999
AspLEI GCGC 5 cut(s) 106, 112, 217, 293, 1667
AspS9I GGNCC 7 cut(s) 64, 142, 456, 553, 554, 967, 1641
AsuC2I CCSGG 7 cut(s) 130, 181, 460, 534, 535, 1293, 1640
AsuII TTCGAA 2 cut(s) 425, 1181
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 4 cut(s) 64, 142, 456, 967
AxyI CCTNAGG 1 cut(s) 1190
BaeGI GKGCMC 4 cut(s) 557, 1293, 1332, 1493
BalI TGGCCA 1 cut(s) 495
BamHI GGATCC 2 cut(s) 733, 1679
BanI GGYRCC 3 cut(s) 103, 1601, 1635
BanII GRGCYC 2 cut(s) 204, 557
BauI CACGAG 1 cut(s) 1687
Bbv12I GWGCWC 3 cut(s) 204, 1332, 1493
BbvI GCAGC 7 cut(s) 245, 469, 596, 673, 826, 1286, 1537
BccI CCATC 3 cut(s) 76, 133, 1304
BceAI ACGGC 8 cut(s) 64, 70, 111, 221, 378, 390, 1332, 1376
BciT130I CCWGG 5 cut(s) 146, 209, 521, 696, 895
BcnI CCSGG 7 cut(s) 130, 181, 460, 534, 535, 1293, 1640
BcoDI GTCTC 2 cut(s) 353, 1421
BfmI CTRYAG 1 cut(s) 582
BfoI RGCGCY 3 cut(s) 107, 113, 294
BfuAI ACCTGC 2 cut(s) 163, 259
BlpI GCTNAGC 1 cut(s) 539
Bme18I GGWCC 4 cut(s) 64, 142, 456, 967
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 7 cut(s) 64, 142, 456, 553, 554, 967, 1641
BmiI GGNNCC 8 cut(s) 66, 105, 555, 735, 968, 1603, 1637, 1681
BmsI GCATC 7 cut(s) 107, 400, 820, 826, 840, 1546, 1683
BpmI CTGGAG 1 cut(s) 42
Bpu1102I GCTNAGC 1 cut(s) 539
Bpu14I TTCGAA 2 cut(s) 425, 1181
BpuEI CTTGAG 1 cut(s) 1666
BpuMI CCSGG 7 cut(s) 130, 181, 460, 534, 535, 1293, 1640
BsaHI GRCGYC 1 cut(s) 104
BsaJI CCNNGG 4 cut(s) 532, 533, 695, 1082
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 211, 1084, 1689
Bse118I RCCGGY 2 cut(s) 106, 1533
Bse21I CCTNAGG 1 cut(s) 1190
Bse3DI GCAATG 4 cut(s) 719, 952, 987, 1368
BseBI CCWGG 5 cut(s) 146, 209, 521, 696, 895
BseDI CCNNGG 4 cut(s) 532, 533, 695, 1082
BseGI GGATG 4 cut(s) 144, 403, 1093, 1698
BseLI CCNNNNNNNGG 4 cut(s) 135, 211, 1084, 1689
BseMI GCAATG 4 cut(s) 719, 952, 987, 1368
BseMII CTCAG 6 cut(s) 285, 1085, 1124, 1204, 1245, 1713
BseRI GAGGAG 2 cut(s) 1504, 1687
BseSI GKGCMC 4 cut(s) 557, 1293, 1332, 1493
BseXI GCAGC 7 cut(s) 245, 469, 596, 673, 826, 1286, 1537
BseYI CCCAGC 1 cut(s) 352
Bsh1236I CGCG 1 cut(s) 1481
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 6 cut(s) 495, 555, 1391, 1511, 1533, 1643
BshNI GGYRCC 3 cut(s) 103, 1601, 1635
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 3 cut(s) 204, 1332, 1493
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 8 cut(s) 107, 129, 180, 459, 534, 1293, 1534, 1639
BslFI GGGAC 5 cut(s) 292, 453, 811, 980, 1327
BslI CCNNNNNNNGG 4 cut(s) 135, 211, 1084, 1689
BsmAI GTCTC 2 cut(s) 353, 1421
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 5 cut(s) 292, 453, 811, 980, 1327
BsmI GAATGC 3 cut(s) 568, 676, 1502
BsnI GGCC 6 cut(s) 495, 555, 1391, 1511, 1533, 1643
BsoBI CYCGRG 1 cut(s) 533
Bsp119I TTCGAA 2 cut(s) 425, 1181
Bsp120I GGGCCC 1 cut(s) 553
Bsp1286I GDGCHC 5 cut(s) 204, 557, 1293, 1332, 1493
Bsp143I GATC 8 cut(s) 12, 226, 523, 656, 733, 1113, 1679, 1723
Bsp1720I GCTNAGC 1 cut(s) 539
BspANI GGCC 6 cut(s) 495, 555, 1391, 1511, 1533, 1643
BspCNI CTCAG 6 cut(s) 286, 1086, 1123, 1203, 1244, 1712
BspFNI CGCG 1 cut(s) 1481
BspLI GGNNCC 8 cut(s) 66, 105, 555, 735, 968, 1603, 1637, 1681
BspMAI CTGCAG 1 cut(s) 586
BspMI ACCTGC 2 cut(s) 163, 259
BspPI GGATC 5 cut(s) 531, 728, 741, 1674, 1687
BspQI GCTCTTC 1 cut(s) 990
BspT104I TTCGAA 2 cut(s) 425, 1181
BspT107I GGYRCC 3 cut(s) 103, 1601, 1635
BsrBI CCGCTC 3 cut(s) 288, 908, 980
BsrDI GCAATG 4 cut(s) 719, 952, 987, 1368
BsrFI RCCGGY 2 cut(s) 106, 1533
BssAI RCCGGY 2 cut(s) 106, 1533
BssECI CCNNGG 4 cut(s) 532, 533, 695, 1082
BssMI GATC 8 cut(s) 12, 226, 523, 656, 733, 1113, 1679, 1723
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 1687
BssT1I CCWWGG 1 cut(s) 1082
Bst2BI CACGAG 1 cut(s) 1687
Bst2UI CCWGG 5 cut(s) 146, 209, 521, 696, 895
Bst4CI ACNGT 8 cut(s) 283, 621, 1052, 1105, 1240, 1264, 1288, 1567
Bst6I CTCTTC 3 cut(s) 128, 990, 1339
BstACI GRCGYC 1 cut(s) 104
BstAPI GCANNNNNTGC 3 cut(s) 250, 839, 1265
BstBI TTCGAA 2 cut(s) 425, 1181
BstC8I GCNNGC 5 cut(s) 108, 906, 1036, 1479, 1535
BstDEI CTNAG 7 cut(s) 294, 539, 1094, 1110, 1190, 1231, 1699
BstEII GGTNACC 1 cut(s) 175
BstF5I GGATG 4 cut(s) 144, 403, 1093, 1698
BstFNI CGCG 1 cut(s) 1481
BstH2I RGCGCY 3 cut(s) 107, 113, 294
BstHHI GCGC 5 cut(s) 106, 112, 217, 293, 1667
BstKTI GATC 8 cut(s) 15, 229, 526, 659, 736, 1116, 1682, 1726
BstMAI GTCTC 2 cut(s) 353, 1421
BstMBI GATC 8 cut(s) 12, 226, 523, 656, 733, 1113, 1679, 1723
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 6 cut(s) 250, 839, 1061, 1265, 1274, 1508
BstNI CCWGG 5 cut(s) 146, 209, 521, 696, 895
BstNSI RCATGY 2 cut(s) 704, 887
BstPI GGTNACC 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 582
BstSLI GKGCMC 4 cut(s) 557, 1293, 1332, 1493
BstUI CGCG 1 cut(s) 1481
BstV1I GCAGC 7 cut(s) 245, 469, 596, 673, 826, 1286, 1537
BstX2I RGATCY 2 cut(s) 733, 1679
BstYI RGATCY 2 cut(s) 733, 1679
Bsu36I CCTNAGG 1 cut(s) 1190
BsuRI GGCC 6 cut(s) 495, 555, 1391, 1511, 1533, 1643
BtsCI GGATG 4 cut(s) 144, 403, 1093, 1698
BtsI GCAGTG 1 cut(s) 1596
BtsIMutI CAGTG 6 cut(s) 956, 1110, 1245, 1491, 1503, 1596
BveI ACCTGC 2 cut(s) 163, 259
Cac8I GCNNGC 5 cut(s) 108, 906, 1036, 1479, 1535
CaiI CAGNNNCTG 2 cut(s) 250, 839
CfoI GCGC 5 cut(s) 106, 112, 217, 293, 1667
Cfr10I RCCGGY 2 cut(s) 106, 1533
Cfr13I GGNCC 7 cut(s) 64, 142, 456, 553, 554, 967, 1641
Cfr9I CCCGGG 1 cut(s) 533
CpoI CGGWCCG 1 cut(s) 456
CseI GACGC 1 cut(s) 1470
Csp6I GTAC 1 cut(s) 515
CspI CGGWCCG 1 cut(s) 456
CviAII CATG 6 cut(s) 491, 500, 701, 884, 1507, 1606
CviQI GTAC 1 cut(s) 515
DdeI CTNAG 7 cut(s) 294, 539, 1094, 1110, 1190, 1231, 1699
DinI GGCGCC 1 cut(s) 105
DpnI GATC 8 cut(s) 14, 228, 525, 658, 735, 1115, 1681, 1725
DpnII GATC 8 cut(s) 12, 226, 523, 656, 733, 1113, 1679, 1723
EaeI YGGCCR 3 cut(s) 493, 1389, 1531
Eam1104I CTCTTC 3 cut(s) 128, 990, 1339
EarI CTCTTC 3 cut(s) 128, 990, 1339
EciI GGCGGA 2 cut(s) 329, 395
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 1082
Eco24I GRGCYC 2 cut(s) 204, 557
Eco47I GGWCC 4 cut(s) 64, 142, 456, 967
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 2 cut(s) 1014, 1479
Eco81I CCTNAGG 1 cut(s) 1190
Eco88I CYCGRG 1 cut(s) 533
Eco91I GGTNACC 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 175
EcoRII CCWGG 5 cut(s) 144, 207, 519, 694, 893
EcoT14I CCWWGG 1 cut(s) 1082
EcoT22I ATGCAT 1 cut(s) 676
EcoT38I GRGCYC 2 cut(s) 204, 557
EgeI GGCGCC 1 cut(s) 105
EheI GGCGCC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 1082
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 6 cut(s) 494, 503, 704, 887, 1510, 1609
FaqI GGGAC 5 cut(s) 292, 453, 811, 980, 1327
FatI CATG 6 cut(s) 490, 499, 700, 883, 1506, 1605
FauI CCCGC 1 cut(s) 142
FauNDI CATATG 1 cut(s) 783
FokI GGATG 4 cut(s) 151, 410, 1100, 1705
FriOI GRGCYC 2 cut(s) 204, 557
GlaI GCGC 5 cut(s) 105, 111, 216, 292, 1666
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 1 cut(s) 42
HaeII RGCGCY 3 cut(s) 107, 113, 294
HaeIII GGCC 6 cut(s) 495, 555, 1391, 1511, 1533, 1643
HapII CCGG 8 cut(s) 107, 129, 180, 459, 534, 1293, 1534, 1639
HgaI GACGC 1 cut(s) 1470
HhaI GCGC 5 cut(s) 106, 112, 217, 293, 1667
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 6 cut(s) 494, 503, 704, 887, 1510, 1609
Hin6I GCGC 5 cut(s) 104, 110, 215, 291, 1665
HinP1I GCGC 5 cut(s) 104, 110, 215, 291, 1665
HinfI GANTC 9 cut(s) 55, 247, 302, 451, 632, 1016, 1108, 1212, 1728
HpaII CCGG 8 cut(s) 107, 129, 180, 459, 534, 1293, 1534, 1639
Hpy166II GTNNAC 4 cut(s) 372, 919, 1330, 1491
Hpy188I TCNGA 7 cut(s) 307, 1012, 1113, 1173, 1421, 1702, 1717
Hpy188III TCNNGA 8 cut(s) 395, 527, 654, 827, 857, 983, 1117, 1192
Hpy8I GTNNAC 4 cut(s) 372, 919, 1330, 1491
Hpy99I CGWCG 3 cut(s) 85, 97, 562
HpyAV CCTTC 6 cut(s) 34, 214, 400, 744, 1202, 1648
HpyCH4III ACNGT 8 cut(s) 283, 621, 1052, 1105, 1240, 1264, 1288, 1567
HpyF10VI GCNNNNNNNGC 6 cut(s) 250, 839, 1061, 1265, 1274, 1508
HpyF3I CTNAG 7 cut(s) 294, 539, 1094, 1110, 1190, 1231, 1699
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 6 cut(s) 494, 503, 704, 887, 1510, 1609
HspAI GCGC 5 cut(s) 104, 110, 215, 291, 1665
KasI GGCGCC 1 cut(s) 103
KroI GCCGGC 2 cut(s) 106, 1533
KroNI GCCGGC 2 cut(s) 108, 1535
Kzo9I GATC 8 cut(s) 12, 226, 523, 656, 733, 1113, 1679, 1723
LguI GCTCTTC 1 cut(s) 990
LmnI GCTCC 2 cut(s) 207, 1203
Lsp1109I GCAGC 7 cut(s) 245, 469, 596, 673, 826, 1286, 1537
LweI GCATC 7 cut(s) 107, 400, 820, 826, 840, 1546, 1683
MaeIII GTNAC 5 cut(s) 175, 869, 1105, 1234, 1561
MalI GATC 8 cut(s) 14, 228, 525, 658, 735, 1115, 1681, 1725
MbiI CCGCTC 3 cut(s) 288, 908, 980
MboI GATC 8 cut(s) 12, 226, 523, 656, 733, 1113, 1679, 1723
MfeI CAATTG 2 cut(s) 740, 843
MflI RGATCY 2 cut(s) 733, 1679
MhlI GDGCHC 5 cut(s) 204, 557, 1293, 1332, 1493
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 9 cut(s) 740, 818, 843, 863, 1407, 1455, 1554, 1711, 1719
MluNI TGGCCA 1 cut(s) 495
Mly113I GGCGCC 1 cut(s) 104
MlyI GAGTC 2 cut(s) 49, 1102
MmeI TCCRAC 3 cut(s) 279, 624, 1707
Mox20I TGGCCA 1 cut(s) 495
Mph1103I ATGCAT 1 cut(s) 676
MreI CGCCGGCG 1 cut(s) 106
MroNI GCCGGC 2 cut(s) 106, 1533
MroXI GAANNNNTTC 1 cut(s) 999
MscI TGGCCA 1 cut(s) 495
MseI TTAA 3 cut(s) 792, 951, 1362
MslI CAYNNNNRTG 3 cut(s) 396, 1241, 1496
Msp20I TGGCCA 1 cut(s) 495
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 8 cut(s) 107, 129, 180, 459, 534, 1293, 1534, 1639
MunI CAATTG 2 cut(s) 740, 843
Mva1269I GAATGC 3 cut(s) 568, 676, 1502
MvaI CCWGG 5 cut(s) 146, 209, 521, 696, 895
MvnI CGCG 1 cut(s) 1481
MwoI GCNNNNNNNGC 6 cut(s) 250, 839, 1061, 1265, 1274, 1508
NaeI GCCGGC 2 cut(s) 108, 1535
NarI GGCGCC 1 cut(s) 104
NciI CCSGG 7 cut(s) 130, 181, 460, 534, 535, 1293, 1640
NdeI CATATG 1 cut(s) 783
NdeII GATC 8 cut(s) 12, 226, 523, 656, 733, 1113, 1679, 1723
NgoMIV GCCGGC 2 cut(s) 106, 1533
NlaIII CATG 6 cut(s) 494, 503, 704, 887, 1510, 1609
NlaIV GGNNCC 8 cut(s) 66, 105, 555, 735, 968, 1603, 1637, 1681
NmuCI GTSAC 3 cut(s) 175, 1105, 1234
NsiI ATGCAT 1 cut(s) 676
NspI RCATGY 2 cut(s) 704, 887
NspV TTCGAA 2 cut(s) 425, 1181
OliI CACNNNNGTG 2 cut(s) 1241, 1496
PaqCI CACCTGC 2 cut(s) 163, 259
PciI ACATGT 1 cut(s) 883
PciSI GCTCTTC 1 cut(s) 990
PcsI WCGNNNNNNNCGW 3 cut(s) 83, 89, 557
PctI GAATGC 3 cut(s) 568, 676, 1502
PdiI GCCGGC 2 cut(s) 108, 1535
PdmI GAANNNNTTC 1 cut(s) 999
PfeI GAWTC 7 cut(s) 247, 302, 451, 632, 1016, 1212, 1728
PflMI CCANNNNNTGG 1 cut(s) 1689
PfoI TCCNGGA 2 cut(s) 128, 519
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 2 cut(s) 49, 1102
PluTI GGCGCC 1 cut(s) 107
PpsI GAGTC 2 cut(s) 49, 1102
PscI ACATGT 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 5 cut(s) 144, 207, 519, 694, 893
PspEI GGTNACC 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 5 cut(s) 144, 207, 519, 694, 893
PspN4I GGNNCC 8 cut(s) 66, 105, 555, 735, 968, 1603, 1637, 1681
PspOMI GGGCCC 1 cut(s) 553
PspPI GGNCC 7 cut(s) 64, 142, 456, 553, 554, 967, 1641
PstI CTGCAG 1 cut(s) 586
PstNI CAGNNNCTG 2 cut(s) 250, 839
PsuI RGATCY 2 cut(s) 733, 1679
PvuI CGATCG 1 cut(s) 15
RsaI GTAC 1 cut(s) 516
RsaNI GTAC 1 cut(s) 515
RseI CAYNNNNRTG 3 cut(s) 396, 1241, 1496
Rsr2I CGGWCCG 1 cut(s) 456
RsrII CGGWCCG 1 cut(s) 456
SacI GAGCTC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 990
SaqAI TTAA 3 cut(s) 792, 951, 1362
Sau3AI GATC 8 cut(s) 12, 226, 523, 656, 733, 1113, 1679, 1723
Sau96I GGNCC 7 cut(s) 64, 142, 456, 553, 554, 967, 1641
SchI GAGTC 2 cut(s) 49, 1102
SduI GDGCHC 5 cut(s) 204, 557, 1293, 1332, 1493
SfaNI GCATC 7 cut(s) 107, 400, 820, 826, 840, 1546, 1683
SfcI CTRYAG 1 cut(s) 582
SfoI GGCGCC 1 cut(s) 105
SfuI TTCGAA 2 cut(s) 425, 1181
SgrAI CRCCGGYG 1 cut(s) 106
SinI GGWCC 4 cut(s) 64, 142, 456, 967
SmaI CCCGGG 1 cut(s) 535
SmiMI CAYNNNNRTG 3 cut(s) 396, 1241, 1496
SmlI CTYRAG 1 cut(s) 1645
SmoI CTYRAG 1 cut(s) 1645
Sse9I AATT 9 cut(s) 740, 818, 843, 863, 1407, 1455, 1554, 1711, 1719
SspDI GGCGCC 1 cut(s) 103
SspI AATATT 1 cut(s) 1177
SstI GAGCTC 1 cut(s) 204
StyI CCWWGG 1 cut(s) 1082
TaaI ACNGT 8 cut(s) 283, 621, 1052, 1105, 1240, 1264, 1288, 1567
TaqI TCGA 7 cut(s) 15, 92, 122, 425, 560, 1181, 1726
TaqII GACCGA 1 cut(s) 444
TasI AATT 9 cut(s) 740, 818, 843, 863, 1407, 1455, 1554, 1711, 1719
TauI GCSGC 6 cut(s) 187, 215, 291, 329, 380, 1636
TfiI GAWTC 7 cut(s) 247, 302, 451, 632, 1016, 1212, 1728
Tru1I TTAA 3 cut(s) 792, 951, 1362
Tru9I TTAA 3 cut(s) 792, 951, 1362
TscAI CASTG 6 cut(s) 963, 1110, 1245, 1491, 1503, 1596
TseFI GTSAC 3 cut(s) 175, 1105, 1234
TseI GCWGC 7 cut(s) 233, 482, 584, 686, 839, 1274, 1550
Tsp45I GTSAC 3 cut(s) 175, 1105, 1234
TspDTI ATGAA 5 cut(s) 630, 1104, 1118, 1345, 1495
TspMI CCCGGG 1 cut(s) 533
TspRI CASTG 6 cut(s) 963, 1110, 1245, 1491, 1503, 1596
Van91I CCANNNNNTGG 1 cut(s) 1689
VneI GTGCAC 2 cut(s) 1328, 1489
VpaK11BI GGWCC 4 cut(s) 64, 142, 456, 967
XapI RAATTY 1 cut(s) 863
XceI RCATGY 2 cut(s) 704, 887
XmaI CCCGGG 1 cut(s) 533
XmnI GAANNNNTTC 1 cut(s) 999
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.