RchiOBHm_Chr1g0368291

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0011641
Physical Location & Seq
Forward (+)
58476729 .. 58480090
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 807 bp
ATGGCGGAGAGCCTCGCAAGTCCGAGATGGTCCCTCAGGGGAACGACTGCCCTCGTCACCGGCGGCACTCGTGGAATTGGGTACGCCGCAGTGGAGGAGCTAGCTGCGCTTGGTGCGTTTGTGTTCACCTGTTCTCGGAACTCAGCAGAACTCGAAAGGTGCCTTAAGGAATGGTCCGGTAAGGGGCTCCGTGTCACTGGGGTTGTCTGCGATGTTTCGACTAAATCCGAGAGAGAAAAACTAATGGAGCAAGTGGGTGTTTCTTTCACTGGGAAGCTCAATATATTTGTAAACAATGTTGGGACAAACATAAGGAAGCCAACTACTGAGTACACTTCGGAAGAGTATTCAAAAGTAATGGCTACTAATTTGGAATCCACATACAATCTGTGCCAACTTGCGCATCCGCTTTTGAAAGCATCTGGAGCAGGAAGCATTGTGTTCATATCATCTGTGGCTGGTTCGGTGAGCATTGGTTCCGGGACCATTTATGCAGCAACTAAAGCTGCGATCAATCAGCTCACGAAAAATCTGGCATGTGAATGGGCGAAAGACAATATCAGGAGCAATGCTGTTGCTCCTTGGTACACCAGAACCTCACTCGTTGAACACATGCTGGAGGACAAGAAGTTTGTGGAAGAGATAACCTCTCGTACCCCTCTCAATCGTGTTGCAGAACCTGAGGAGGTCTCATCCTTGGTAGCCTTCCTCTGCCTACCAGCTGCTTCATACATCACCGGACAGATTATATCTGTTGATGGAGGCATGACTGCTAACAGTTTTTGCCCCTCTAAGATACCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

268

Amino Acids

28.76

Weight (kDa)

7.53

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDR PF23441 12 - 256 1e-07 SDR-like rossmann domain
adh_short PF00106 16 - 208 1.3e-52 short chain dehydrogenase
KR PF08659 16 - 176 3.3e-14 KR domain
Epimerase PF01370 17 - 180 1.6e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 24 - 257 2.5e-61 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011641)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06060
fragaria_vesca FvH4_7g23050 FvH4_7g23050
malus_domestica MD01G1139100.v1.1
prunus_persica Prupe.2G239600_v2.0.a1 Prupe.2G239600_v2.0.a1 Prupe.2G239600_v2.0.a1
pyrus_communis pycom01g15970
rosa_chinensis RchiOBHm_Chr1g0368291
rosa_laevigata RLG00000027223
rosa_multiflora Rmu_sc0006033.1_g000017 Rmu_sc0009391.1_g000003
rosa_roxburghii Rroxscaffold_4G00288680
rosa_rugosa Rorug01G0339900
rosa_samantha Rh1AG347400 Rh1BG310400 Rh1CG325800 Rh1DG342200
rosa_wichuraiana Rw1G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 159
AciI CCGC 4 cut(s) 5, 63, 87, 407
AfaI GTAC 4 cut(s) 83, 332, 587, 655
AfiI CCNNNNNNNGG 2 cut(s) 135, 183
AflII CTTAAG 1 cut(s) 164
AgsI TTSAA 3 cut(s) 351, 415, 608
AluBI AGCT 6 cut(s) 100, 104, 277, 506, 520, 722
AluI AGCT 6 cut(s) 100, 104, 277, 506, 520, 722
Alw26I GTCTC 1 cut(s) 694
AlwNI CAGNNNCTG 1 cut(s) 680
ApeKI GCWGC 4 cut(s) 104, 494, 506, 722
ArsI GACNNNNNNTTYG 2 cut(s) 614, 646
AspLEI GCGC 2 cut(s) 109, 403
AspS9I GGNCC 3 cut(s) 30, 174, 483
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 4 cut(s) 49, 118, 478, 727
AsuNHI GCTAGC 1 cut(s) 100
AvaII GGWCC 3 cut(s) 30, 174, 483
AxyI CCTNAGG 2 cut(s) 35, 681
BanI GGYRCC 1 cut(s) 159
BanII GRGCYC 1 cut(s) 189
BauI CACGAG 1 cut(s) 69
BbvI GCAGC 4 cut(s) 91, 493, 506, 709
BccI CCATC 2 cut(s) 21, 752
BcnI CCSGG 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 694
BfaI CTAG 1 cut(s) 101
BfrI CTTAAG 1 cut(s) 164
BisI GCNGC 6 cut(s) 64, 87, 105, 495, 507, 723
BlsI GCNGC 6 cut(s) 65, 88, 106, 496, 508, 724
Bme1390I CCNGG 1 cut(s) 481
Bme18I GGWCC 3 cut(s) 30, 174, 483
BmgT120I GGNCC 3 cut(s) 30, 174, 483
BmiI GGNNCC 5 cut(s) 32, 161, 188, 478, 484
BmrFI CCNGG 1 cut(s) 481
BmrI ACTGGG 2 cut(s) 207, 279
BmsI GCATC 2 cut(s) 412, 428
BmtI GCTAGC 1 cut(s) 104
BmuI ACTGGG 2 cut(s) 207, 279
BplI GAGNNNNNCTC 4 cut(s) 632, 664, 674, 706
BpmI CTGGAG 2 cut(s) 444, 638
BpuMI CCSGG 1 cut(s) 481
BsaI GGTCTC 1 cut(s) 694
BsaJI CCNNGG 2 cut(s) 581, 696
BsaWI WCCGGW 2 cut(s) 176, 737
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 183
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 2 cut(s) 202, 274
Bse21I CCTNAGG 2 cut(s) 35, 681
Bse3DI GCAATG 1 cut(s) 574
BseDI CCNNGG 2 cut(s) 581, 696
BseGI GGATG 2 cut(s) 403, 692
BseLI CCNNNNNNNGG 2 cut(s) 135, 183
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 4 cut(s) 49, 156, 318, 672
BseNI ACTGG 2 cut(s) 202, 274
BseRI GAGGAG 2 cut(s) 110, 698
BseXI GCAGC 4 cut(s) 91, 493, 506, 709
BshNI GGYRCC 1 cut(s) 159
BsiSI CCGG 4 cut(s) 60, 177, 480, 738
BslFI GGGAC 3 cut(s) 16, 316, 496
BslI CCNNNNNNNGG 2 cut(s) 135, 183
BsmAI GTCTC 1 cut(s) 694
BsmFI GGGAC 3 cut(s) 16, 316, 496
Bso31I GGTCTC 1 cut(s) 694
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 1 cut(s) 510
BspACI CCGC 4 cut(s) 5, 63, 87, 407
BspCNI CTCAG 4 cut(s) 48, 155, 319, 673
BspLI GGNNCC 5 cut(s) 32, 161, 188, 478, 484
BspOI GCTAGC 1 cut(s) 104
BspT107I GGYRCC 1 cut(s) 159
BspTI CTTAAG 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 694
BsrDI GCAATG 1 cut(s) 574
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 2 cut(s) 202, 274
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 2 cut(s) 581, 696
BssMI GATC 1 cut(s) 510
BssSI CACGAG 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 581, 696
Bst2BI CACGAG 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 779
Bst6I CTCTTC 2 cut(s) 336, 633
BstAFI CTTAAG 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 5 cut(s) 35, 142, 327, 681, 792
BstF5I GGATG 2 cut(s) 403, 692
BstHHI GCGC 2 cut(s) 109, 403
BstKTI GATC 1 cut(s) 513
BstMAI GTCTC 1 cut(s) 694
BstMBI GATC 1 cut(s) 510
BstMWI GCNNNNNNNGC 4 cut(s) 106, 113, 425, 503
BstNSI RCATGY 2 cut(s) 540, 616
BstSCI CCNGG 1 cut(s) 479
BstV1I GCAGC 4 cut(s) 91, 493, 506, 709
Bsu36I CCTNAGG 2 cut(s) 35, 681
BtgZI GCGATG 1 cut(s) 225
BtsCI GGATG 2 cut(s) 403, 692
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 3 cut(s) 96, 195, 267
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 2 cut(s) 109, 403
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 3 cut(s) 30, 174, 483
Csp6I GTAC 4 cut(s) 82, 331, 586, 654
CviAII CATG 3 cut(s) 537, 613, 766
CviQI GTAC 4 cut(s) 82, 331, 586, 654
DdeI CTNAG 5 cut(s) 35, 142, 327, 681, 792
DpnI GATC 1 cut(s) 512
DpnII GATC 1 cut(s) 510
Eam1104I CTCTTC 2 cut(s) 336, 633
EarI CTCTTC 2 cut(s) 336, 633
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 581, 696
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 694
Eco47I GGWCC 3 cut(s) 30, 174, 483
Eco81I CCTNAGG 2 cut(s) 35, 681
EcoT14I CCWWGG 2 cut(s) 581, 696
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 2 cut(s) 581, 696
FaeI CATG 3 cut(s) 540, 616, 769
FaqI GGGAC 3 cut(s) 16, 316, 496
FatI CATG 3 cut(s) 536, 612, 765
Fnu4HI GCNGC 6 cut(s) 64, 87, 105, 495, 507, 723
FokI GGATG 2 cut(s) 390, 679
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 6 cut(s) 64, 87, 105, 495, 507, 723
FspBI CTAG 1 cut(s) 101
FspI TGCGCA 1 cut(s) 402
GlaI GCGC 2 cut(s) 108, 402
GluI GCNGC 6 cut(s) 64, 87, 105, 495, 507, 723
GsuI CTGGAG 2 cut(s) 444, 638
HapII CCGG 4 cut(s) 60, 177, 480, 738
HhaI GCGC 2 cut(s) 109, 403
Hin1II CATG 3 cut(s) 540, 616, 769
Hin6I GCGC 2 cut(s) 107, 401
HinP1I GCGC 2 cut(s) 107, 401
HinfI GANTC 1 cut(s) 374
HpaII CCGG 4 cut(s) 60, 177, 480, 738
HphI GGTGA 4 cut(s) 49, 118, 478, 727
Hpy166II GTNNAC 4 cut(s) 126, 292, 333, 588
Hpy188I TCNGA 4 cut(s) 24, 138, 229, 340
Hpy188III TCNNGA 3 cut(s) 423, 523, 562
Hpy8I GTNNAC 4 cut(s) 126, 292, 333, 588
HpyAV CCTTC 1 cut(s) 715
HpyCH4III ACNGT 1 cut(s) 779
HpyCH4V TGCA 2 cut(s) 494, 674
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 113, 425, 503
HpyF3I CTNAG 5 cut(s) 35, 142, 327, 681, 792
Hsp92II CATG 3 cut(s) 540, 616, 769
HspAI GCGC 2 cut(s) 107, 401
Kzo9I GATC 1 cut(s) 510
LmnI GCTCC 6 cut(s) 97, 192, 247, 425, 564, 583
Lsp1109I GCAGC 4 cut(s) 91, 493, 506, 709
LweI GCATC 2 cut(s) 412, 428
MaeI CTAG 1 cut(s) 101
MaeIII GTNAC 2 cut(s) 55, 193
MalI GATC 1 cut(s) 512
MboI GATC 1 cut(s) 510
MboII GAAGA 2 cut(s) 353, 650
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 2 cut(s) 75, 367
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 541
MspA1I CMGCKG 1 cut(s) 722
MspCI CTTAAG 1 cut(s) 164
MspI CCGG 4 cut(s) 60, 177, 480, 738
MspR9I CCNGG 1 cut(s) 481
MwoI GCNNNNNNNGC 4 cut(s) 106, 113, 425, 503
NciI CCSGG 1 cut(s) 481
NdeII GATC 1 cut(s) 510
NheI GCTAGC 1 cut(s) 100
NlaIII CATG 3 cut(s) 540, 616, 769
NlaIV GGNNCC 5 cut(s) 32, 161, 188, 478, 484
NmuCI GTSAC 2 cut(s) 55, 193
NsbI TGCGCA 1 cut(s) 402
NspI RCATGY 2 cut(s) 540, 616
PfeI GAWTC 1 cut(s) 374
PfoI TCCNGGA 1 cut(s) 479
PkrI GCNGC 6 cut(s) 65, 88, 106, 496, 508, 724
PspN4I GGNNCC 5 cut(s) 32, 161, 188, 478, 484
PspPI GGNCC 3 cut(s) 30, 174, 483
PstNI CAGNNNCTG 1 cut(s) 680
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 4 cut(s) 83, 332, 587, 655
RsaNI GTAC 4 cut(s) 82, 331, 586, 654
RseI CAYNNNNRTG 1 cut(s) 541
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 6 cut(s) 64, 87, 105, 495, 507, 723
Sau3AI GATC 1 cut(s) 510
Sau96I GGNCC 3 cut(s) 30, 174, 483
ScrFI CCNGG 1 cut(s) 481
SduI GDGCHC 1 cut(s) 189
SfaNI GCATC 2 cut(s) 412, 428
SgrAI CRCCGGYG 1 cut(s) 59
SinI GGWCC 3 cut(s) 30, 174, 483
SmiMI CAYNNNNRTG 1 cut(s) 541
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 75, 367
SsiI CCGC 4 cut(s) 5, 63, 87, 407
SspMI CTAG 1 cut(s) 101
StyD4I CCNGG 1 cut(s) 479
StyI CCWWGG 2 cut(s) 581, 696
TaaI ACNGT 1 cut(s) 779
TaqI TCGA 2 cut(s) 153, 218
TasI AATT 2 cut(s) 75, 367
TatI WGTACW 1 cut(s) 330
TauI GCSGC 2 cut(s) 66, 89
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 3 cut(s) 96, 202, 274
TseFI GTSAC 2 cut(s) 55, 193
TseI GCWGC 4 cut(s) 104, 494, 506, 722
Tsp45I GTSAC 2 cut(s) 55, 193
TspDTI ATGAA 2 cut(s) 433, 717
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 3 cut(s) 96, 202, 274
Vha464I CTTAAG 1 cut(s) 164
VpaK11BI GGWCC 3 cut(s) 30, 174, 483
XceI RCATGY 2 cut(s) 540, 616
XspI CTAG 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.