RchiOBHm_Chr1g0368711

DA1-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002660
Physical Location & Seq
Forward (+)
58713341 .. 58717280
3940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1527 bp
ATGGCTCCCTCAGGTGTCAACCAGCTCTCTCACCCTTGCGTTTATGGGGAGTTTGTTTCTTCATACGCAGAGAGGAAGTCACGCTTTATGAAATGGCTGAGTAAACTTTTCAGGAGCGGGAACAATCGAGGAGGAACTTCTGGGAATCGCAATCATCAGCTTATTGGCGACGAAAACATGGTTTGGCGTGCGCCTTTTAGATCTGTGGATGAGCGGTCTAGAACCCAGAAAGAGAAAGAGGAATTAGACCATGCAATTGCGCTCTCTCTGGCTGAAGATTCAAAGAGACACAGAGGATATAGATGGCAGGACAAAGATAACGAGCTAGCTAGGTCACTTCAGGATATAAATCCACCAATATATCCTCCACATGCCCCTCCAGTTCCTCAACATAATCCGTATGCAGTTCCTCAACATAATACAATATGTGGTGGTTGTCAGCAAGATATACGTTACGGTAATTATTTGGGCTGCATGGGGACATTTTTTCATCCTCAGTGCTTCTGCTGTCGTTCTTGTGGTCGCCCAATTGTTGAGAATGAGTTTTCTCTATCAGGGAGGGATCCTTATCATAAGTCCTGTTTTAAGGAGCTGGCCCATCCAAAATGTGAAGTTTGTCATCAATTTATTCCAACGAATCAAGCTGGTTTGATCGAGTATAGATGCCATCCATTTTGGTCACAAAAATACTGTCCATCACATGAACATGATAACACAGCTCGTTGCTGTAGTTGTGAACGCTTAGAGTCTTGGGATGCAAGATATATTTCAGTGGGAGATGGACGGAGTCTATGCTTTGAATGTATGGAATCAGCTATCATGGATACTGGTGATTGTCAGCCATTGTATCATGCCATCAGAGATTATTATGAAGGAATGAATATGAAATTAGATCAGCAAATTCCCATGCTTCTGGTTGAAAGACAAGCACTTAACGAAGCTATTGTCGGGGAGAAGAATGGCCTCCATCACATGCCCGAGACAAGGGGTTTATGCCTTTCTGAAGAGCAGACAGTCACCAGTATACTCAAAAGGCCAAGAATTGGAGGGCATCAACTTATCGGAATGAGAACCCAACCTCAAAAGTTGACTCGAAGATGTGAAGTTACTGCCATTCTTGTTCTCTATAGCCTTCCAAGATTACTAACAGGTGCTATTCTTGCTCATGAGTTGATGCACGGCTGGTTACGTCTCAAAGGCTACCGTAACCTTAATCCTGAAGTAGAGGAAGGTATCTGTCAAGTGCTTTCATACATGTGGCTTGAAACAGAAGTAATGCCAGGATTCAAAAACATGCCATCCACGTCTACATGTGCAGCTTCTTCTTCCACTTCCTCATCCTCATCGAAAAAGGGTGGAAAGTCGAATGTTGAACAGAAGCTTGGTGAATTTTTCATGCATCAAATTGCGCATGATTCTTCCCCAGCATATGGTGGAGGGTTTAGAGCAGCTAATGCAGCTGTCAATAAGTATGGTTTACGTCGTACTCTGGATCACATTCGACTCACTGGAAATTTCCCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0022622 GO:0032182 GO:0032501 GO:0032502 GO:0043130 GO:0048364 GO:0048731 GO:0048856 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

508

Amino Acids

57.61

Weight (kDa)

8.35

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIM PF00412 143 - 197 5.1e-08 LIM domain
DA1-like PF12315 292 - 503 1.6e-100 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1410
AccB7I CCANNNNNTGG 2 cut(s) 1043, 1430
AccBSI CCGCTC 2 cut(s) 117, 214
AccI GTMKAC 2 cut(s) 1024, 1307
AciI CCGC 2 cut(s) 117, 214
AclWI GGATC 3 cut(s) 557, 570, 1500
AcsI RAATTY 3 cut(s) 900, 1388, 1513
AcuI CTGAAG 4 cut(s) 294, 323, 1023, 1239
AfaI GTAC 1 cut(s) 1486
AfiI CCNNNNNNNGG 3 cut(s) 984, 1043, 1430
AflIII ACRYGT 2 cut(s) 1254, 1310
AgsI TTSAA 6 cut(s) 282, 800, 920, 1265, 1288, 1373
AjiI CACGTC 1 cut(s) 1305
AjnI CCWGG 1 cut(s) 1279
AjuI GAANNNNNNNTTGG 2 cut(s) 1365, 1397
Alw26I GTCTC 3 cut(s) 280, 974, 1196
AlwI GGATC 3 cut(s) 557, 570, 1500
Ama87I CYCGRG 1 cut(s) 977
AoxI GGCC 3 cut(s) 594, 961, 1034
ApeKI GCWGC 4 cut(s) 471, 1316, 1448, 1457
ApoI RAATTY 3 cut(s) 900, 1388, 1513
ArsI GACNNNNNNTTYG 4 cut(s) 676, 708, 930, 962
AspLEI GCGC 3 cut(s) 193, 262, 1411
AspS9I GGNCC 1 cut(s) 595
AsuHPI GGTGA 4 cut(s) 23, 842, 1009, 1397
AsuNHI GCTAGC 1 cut(s) 325
AvaI CYCGRG 1 cut(s) 977
AxyI CCTNAGG 1 cut(s) 10
BamHI GGATCC 1 cut(s) 562
BbvI GCAGC 4 cut(s) 458, 1328, 1460, 1469
BccI CCATC 8 cut(s) 297, 606, 675, 703, 773, 863, 975, 1306
BceAI ACGGC 1 cut(s) 1195
BciT130I CCWGG 1 cut(s) 1281
BciVI GTATCC 1 cut(s) 817
BcoDI GTCTC 3 cut(s) 280, 974, 1196
BfaI CTAG 3 cut(s) 219, 326, 330
BfmI CTRYAG 2 cut(s) 727, 1126
BfuI GTATCC 1 cut(s) 817
BglII AGATCT 1 cut(s) 200
BisI GCNGC 4 cut(s) 472, 1317, 1449, 1458
BlsI GCNGC 4 cut(s) 473, 1318, 1450, 1459
Bme1390I CCNGG 1 cut(s) 1281
BmeT110I CYCGRG 1 cut(s) 977
BmgBI CACGTC 1 cut(s) 1305
BmgT120I GGNCC 1 cut(s) 595
BmiI GGNNCC 2 cut(s) 6, 564
BmrFI CCNGG 1 cut(s) 1281
BmsI GCATC 5 cut(s) 653, 745, 1060, 1164, 1408
BmtI GCTAGC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 363
BsaBI GATNNNNATC 2 cut(s) 348, 567
Bsc4I CCNNNNNNNGG 3 cut(s) 984, 1043, 1430
Bse1I ACTGG 4 cut(s) 380, 832, 1020, 1513
Bse21I CCTNAGG 1 cut(s) 10
Bse8I GATNNNNATC 2 cut(s) 348, 567
BseBI CCWGG 1 cut(s) 1281
BseGI GGATG 7 cut(s) 214, 490, 598, 667, 760, 1298, 1337
BseJI GATNNNNATC 2 cut(s) 348, 567
BseLI CCNNNNNNNGG 3 cut(s) 984, 1043, 1430
BseMII CTCAG 3 cut(s) 24, 89, 509
BseNI ACTGG 4 cut(s) 380, 832, 1020, 1513
BseRI GAGGAG 1 cut(s) 144
BseXI GCAGC 4 cut(s) 458, 1328, 1460, 1469
BseYI CCCAGC 1 cut(s) 1423
BsgI GTGCAG 1 cut(s) 1335
BshFI GGCC 3 cut(s) 596, 963, 1036
BsiHKCI CYCGRG 1 cut(s) 977
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 984, 1043, 1430
BsmAI GTCTC 3 cut(s) 280, 974, 1196
BsmBI CGTCTC 1 cut(s) 1196
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 3 cut(s) 596, 963, 1036
BsoBI CYCGRG 1 cut(s) 977
Bsp143I GATC 5 cut(s) 200, 562, 651, 892, 1492
BspACI CCGC 2 cut(s) 117, 214
BspANI GGCC 3 cut(s) 596, 963, 1036
BspCNI CTCAG 3 cut(s) 23, 90, 508
BspHI TCATGA 1 cut(s) 1165
BspLI GGNNCC 2 cut(s) 6, 564
BspOI GCTAGC 1 cut(s) 329
BspPI GGATC 3 cut(s) 557, 570, 1500
BspQI GCTCTTC 1 cut(s) 999
BsrBI CCGCTC 2 cut(s) 117, 214
BsrI ACTGG 4 cut(s) 380, 832, 1020, 1513
BssMI GATC 5 cut(s) 200, 562, 651, 892, 1492
BssNAI GTATAC 1 cut(s) 1025
Bst1107I GTATAC 1 cut(s) 1025
Bst2UI CCWGG 1 cut(s) 1281
Bst4CI ACNGT 4 cut(s) 458, 692, 1015, 1205
Bst6I CTCTTC 1 cut(s) 999
BstAPI GCANNNNNTGC 1 cut(s) 1454
BstC8I GCNNGC 3 cut(s) 189, 327, 594
BstDEI CTNAG 4 cut(s) 10, 98, 495, 742
BstF5I GGATG 7 cut(s) 214, 490, 598, 667, 760, 1298, 1337
BstHHI GCGC 3 cut(s) 193, 262, 1411
BstKTI GATC 5 cut(s) 203, 565, 654, 895, 1495
BstMAI GTCTC 3 cut(s) 280, 974, 1196
BstMBI GATC 5 cut(s) 200, 562, 651, 892, 1492
BstMWI GCNNNNNNNGC 3 cut(s) 1160, 1454, 1457
BstNI CCWGG 1 cut(s) 1281
BstNSI RCATGY 5 cut(s) 374, 976, 1258, 1297, 1314
BstSCI CCNGG 1 cut(s) 1279
BstSFI CTRYAG 2 cut(s) 727, 1126
BstV1I GCAGC 4 cut(s) 458, 1328, 1460, 1469
BstX2I RGATCY 2 cut(s) 200, 562
BstXI CCANNNNNNTGG 1 cut(s) 913
BstYI RGATCY 2 cut(s) 200, 562
BstZ17I GTATAC 1 cut(s) 1025
Bsu36I CCTNAGG 1 cut(s) 10
BsuI GTATCC 1 cut(s) 817
BsuRI GGCC 3 cut(s) 596, 963, 1036
BtrI CACGTC 1 cut(s) 1305
BtsCI GGATG 7 cut(s) 214, 490, 598, 667, 760, 1298, 1337
BtsIMutI CAGTG 3 cut(s) 503, 777, 1506
Cac8I GCNNGC 3 cut(s) 189, 327, 594
CciI TCATGA 1 cut(s) 1165
CfoI GCGC 3 cut(s) 193, 262, 1411
Cfr13I GGNCC 1 cut(s) 595
Csp6I GTAC 1 cut(s) 1485
CviQI GTAC 1 cut(s) 1485
DdeI CTNAG 4 cut(s) 10, 98, 495, 742
DpnI GATC 5 cut(s) 202, 564, 653, 894, 1494
DpnII GATC 5 cut(s) 200, 562, 651, 892, 1492
Eam1104I CTCTTC 1 cut(s) 999
EarI CTCTTC 1 cut(s) 999
Eco57I CTGAAG 4 cut(s) 294, 323, 1023, 1239
Eco81I CCTNAGG 1 cut(s) 10
Eco88I CYCGRG 1 cut(s) 977
EcoRII CCWGG 1 cut(s) 1279
EcoT22I ATGCAT 1 cut(s) 1401
Esp3I CGTCTC 1 cut(s) 1196
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 493
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 1429
FblI GTMKAC 2 cut(s) 1024, 1307
Fnu4HI GCNGC 4 cut(s) 472, 1317, 1449, 1458
FokI GGATG 7 cut(s) 221, 477, 585, 654, 767, 1285, 1324
Fsp4HI GCNGC 4 cut(s) 472, 1317, 1449, 1458
FspBI CTAG 3 cut(s) 219, 326, 330
FspI TGCGCA 1 cut(s) 1410
GlaI GCGC 3 cut(s) 192, 261, 1410
GluI GCNGC 4 cut(s) 472, 1317, 1449, 1458
GsaI CCCAGC 1 cut(s) 1427
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 3 cut(s) 596, 963, 1036
HhaI GCGC 3 cut(s) 193, 262, 1411
Hin6I GCGC 3 cut(s) 191, 260, 1409
HinP1I GCGC 3 cut(s) 191, 260, 1409
HincII GTYRAC 2 cut(s) 19, 1089
HindII GTYRAC 2 cut(s) 19, 1089
HindIII AAGCTT 1 cut(s) 1379
HphI GGTGA 4 cut(s) 23, 842, 1009, 1397
Hpy166II GTNNAC 7 cut(s) 19, 104, 737, 1025, 1089, 1308, 1478
Hpy188I TCNGA 3 cut(s) 860, 1003, 1064
Hpy188III TCNNGA 6 cut(s) 112, 219, 341, 1166, 1217, 1490
Hpy8I GTNNAC 7 cut(s) 19, 104, 737, 1025, 1089, 1308, 1478
Hpy99I CGWCG 2 cut(s) 173, 1485
HpyAV CCTTC 3 cut(s) 866, 1142, 1223
HpyCH4III ACNGT 4 cut(s) 458, 692, 1015, 1205
HpyCH4IV ACGT 4 cut(s) 451, 1189, 1304, 1480
HpyCH4V TGCA 8 cut(s) 254, 404, 474, 758, 1177, 1316, 1399, 1457
HpyF10VI GCNNNNNNNGC 3 cut(s) 1160, 1454, 1457
HpyF3I CTNAG 4 cut(s) 10, 98, 495, 742
HpySE526I ACGT 4 cut(s) 451, 1189, 1304, 1480
HspAI GCGC 3 cut(s) 191, 260, 1409
Kzo9I GATC 5 cut(s) 200, 562, 651, 892, 1492
LguI GCTCTTC 1 cut(s) 999
LmnI GCTCC 3 cut(s) 10, 114, 589
Lsp1109I GCAGC 4 cut(s) 458, 1328, 1460, 1469
LweI GCATC 5 cut(s) 653, 745, 1060, 1164, 1408
MaeI CTAG 3 cut(s) 219, 326, 330
MaeII ACGT 4 cut(s) 451, 1189, 1304, 1480
MaeIII GTNAC 8 cut(s) 78, 333, 452, 678, 1015, 1105, 1185, 1205
MalI GATC 5 cut(s) 202, 564, 653, 894, 1494
MbiI CCGCTC 2 cut(s) 117, 214
MboI GATC 5 cut(s) 200, 562, 651, 892, 1492
MboII GAAGA 8 cut(s) 51, 287, 967, 1016, 1107, 1314, 1317, 1410
MfeI CAATTG 2 cut(s) 255, 528
MflI RGATCY 2 cut(s) 200, 562
MlyI GAGTC 4 cut(s) 755, 796, 1084, 1497
MmeI TCCRAC 1 cut(s) 656
Mph1103I ATGCAT 1 cut(s) 1401
MseI TTAA 3 cut(s) 585, 933, 1212
MslI CAYNNNNRTG 2 cut(s) 705, 1255
MspA1I CMGCKG 1 cut(s) 1460
MspR9I CCNGG 1 cut(s) 1281
MunI CAATTG 2 cut(s) 255, 528
MvaI CCWGG 1 cut(s) 1281
MwoI GCNNNNNNNGC 3 cut(s) 1160, 1454, 1457
NdeI CATATG 1 cut(s) 1429
NdeII GATC 5 cut(s) 200, 562, 651, 892, 1492
NheI GCTAGC 1 cut(s) 325
NlaIV GGNNCC 2 cut(s) 6, 564
NmuCI GTSAC 4 cut(s) 78, 333, 678, 1015
NsbI TGCGCA 1 cut(s) 1410
NsiI ATGCAT 1 cut(s) 1401
NspI RCATGY 5 cut(s) 374, 976, 1258, 1297, 1314
PagI TCATGA 1 cut(s) 1165
PciI ACATGT 2 cut(s) 1254, 1310
PciSI GCTCTTC 1 cut(s) 999
PfeI GAWTC 6 cut(s) 145, 278, 637, 809, 1284, 1415
PflFI GACNNNGTC 1 cut(s) 786
PflMI CCANNNNNTGG 2 cut(s) 1043, 1430
PkrI GCNGC 4 cut(s) 473, 1318, 1450, 1459
PleI GAGTC 4 cut(s) 754, 795, 1084, 1497
PpsI GAGTC 4 cut(s) 754, 795, 1084, 1497
PscI ACATGT 2 cut(s) 1254, 1310
Psp6I CCWGG 1 cut(s) 1279
PspFI CCCAGC 1 cut(s) 1423
PspGI CCWGG 1 cut(s) 1279
PspN4I GGNNCC 2 cut(s) 6, 564
PspPI GGNCC 1 cut(s) 595
PsuI RGATCY 2 cut(s) 200, 562
PsyI GACNNNGTC 1 cut(s) 786
PvuII CAGCTG 1 cut(s) 1460
RsaI GTAC 1 cut(s) 1486
RsaNI GTAC 1 cut(s) 1485
RseI CAYNNNNRTG 2 cut(s) 705, 1255
SapI GCTCTTC 1 cut(s) 999
SaqAI TTAA 3 cut(s) 585, 933, 1212
SatI GCNGC 4 cut(s) 472, 1317, 1449, 1458
Sau3AI GATC 5 cut(s) 200, 562, 651, 892, 1492
Sau96I GGNCC 1 cut(s) 595
SchI GAGTC 4 cut(s) 755, 796, 1084, 1497
ScrFI CCNGG 1 cut(s) 1281
SfaNI GCATC 5 cut(s) 653, 745, 1060, 1164, 1408
SfcI CTRYAG 2 cut(s) 727, 1126
SmiMI CAYNNNNRTG 2 cut(s) 705, 1255
SsiI CCGC 2 cut(s) 117, 214
SspMI CTAG 3 cut(s) 219, 326, 330
StyD4I CCNGG 1 cut(s) 1279
TaaI ACNGT 4 cut(s) 458, 692, 1015, 1205
TaiI ACGT 4 cut(s) 454, 1192, 1307, 1483
TaqI TCGA 6 cut(s) 127, 654, 1093, 1346, 1364, 1501
TfiI GAWTC 6 cut(s) 145, 278, 637, 809, 1284, 1415
Tru1I TTAA 3 cut(s) 585, 933, 1212
Tru9I TTAA 3 cut(s) 585, 933, 1212
TscAI CASTG 3 cut(s) 503, 777, 1513
TseFI GTSAC 4 cut(s) 78, 333, 678, 1015
TseI GCWGC 4 cut(s) 471, 1316, 1448, 1457
Tsp45I GTSAC 4 cut(s) 78, 333, 678, 1015
TspDTI ATGAA 9 cut(s) 51, 104, 479, 717, 885, 893, 899, 1239, 1384
TspGWI ACGGA 2 cut(s) 387, 799
TspRI CASTG 3 cut(s) 503, 777, 1513
Tth111I GACNNNGTC 1 cut(s) 786
Van91I CCANNNNNTGG 2 cut(s) 1043, 1430
XapI RAATTY 3 cut(s) 900, 1388, 1513
XbaI TCTAGA 1 cut(s) 218
XceI RCATGY 5 cut(s) 374, 976, 1258, 1297, 1314
XmiI GTMKAC 2 cut(s) 1024, 1307
XspI CTAG 3 cut(s) 219, 326, 330
Zsp2I ATGCAT 1 cut(s) 1401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.