RchiOBHm_Chr0c30g0501231

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0016969
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1092 bp
ATGGCGGATTTGGAGATGAGTAGAGCCCGCTATCCATGGCGAGTGTTGGCATTTGATGCGAACGGCGTCATACAAGTTTTGGAGCATTCTGGTGGAGCCTTCGAAGAGCACGTGGTTCAGACTTGGGACAAATCGGATTCCCGAAGCCTAGGTGTGGTTGCCGCCTCTCTGAATGACCGGTCACTAGCTGTGGGTAGAAGAAATGGCACGGTTGAGGTTCGTGATCGTCTCAGTGGGGAGATCGGTCCAACCATCCCTCCTCTCACTAATGAGAGTGATCCTTTTGTGGGGCTGCATCTATTTACAAAGCAGCAGCCAGGATCATTTGGGTCATTGCTTAGTTGTACAGTTCAAGGAAATGCAAGGATAATATCTTTTGAAGTGGACAACTCTTCTAGGACTTTGCTACAATGGAAAGTGAGAGAGAATATCTTGTGTTGCAAGGTTGACGGGAGTGAACGTGTTTGTCTGTTCGGAGGGAAGAATGAAATGAATATATGGGATCTTGAATATAATGTCAAGTCTTGGCCAACAAAAGAGCATCCAATTCAGAATGGAAAACTTTGGTGGACATCTGCCACCTTTCTCACAAGAGATAATCCTCATACATTTGTGGTGGGCACCAACAACAATCAGGTTCATATCTACGATGCTCGCAAGAAGGTTGATGAGTTAAGTCCGTGCAAGATATATGACATAGAAAGCAGCTGTATAAAAAGGCTACCTAACCGTGACACTGCAATACAAGCTATTGCAGAAGGTGAAGATGGCTATACAGTTTATGCAGGCAATGATTGTGGTAATCTTGTTTCTTTGGATACACGCACAGGGAGAGTTTTGGGTTGCTTTCGGGGAATAGACACACTTTCTATAAAATCCTTGGCTAAGCACCACGAGCTTCCTGTGATTGCGTCTTGTGGAGATGACAGCTGTCTCAGCATTTGGGATACAAAGAGGCAAAAACTGCAATGTCGGATCCTTGTGCGTCCGACTATTCGTGTCACAAATGTTATTGTTGATCCCAACTTTTTTGAAAAGGATGGAGGCAAAAAGGGGAAAAGTCACAAAAGGTTCAGAAGTGATGCATCTTGA

Protein Analysis

363

Amino Acids

40.55

Weight (kDa)

8.24

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 199 - 321 4.6e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 620
AciI CCGC 3 cut(s) 5, 28, 162
AclWI GGATC 6 cut(s) 272, 328, 510, 970, 983, 1013
AcoI YGGCCR 1 cut(s) 527
AcvI CACGTG 1 cut(s) 112
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 2 cut(s) 154, 287
AflIII ACRYGT 1 cut(s) 460
AgeI ACCGGT 1 cut(s) 177
AgsI TTSAA 4 cut(s) 353, 380, 509, 1034
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 5 cut(s) 188, 708, 749, 898, 930
AluI AGCT 5 cut(s) 188, 708, 749, 898, 930
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 2 cut(s) 233, 938
AlwI GGATC 6 cut(s) 272, 328, 510, 970, 983, 1013
AoxI GGCC 1 cut(s) 527
ApeKI GCWGC 4 cut(s) 292, 310, 313, 705
AsiGI ACCGGT 1 cut(s) 177
AspA2I CCTAGG 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 773
AsuII TTCGAA 1 cut(s) 102
AvaII GGWCC 1 cut(s) 245
AvrII CCTAGG 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 623
BalI TGGCCA 1 cut(s) 529
BamHI GGATCC 1 cut(s) 975
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 1 cut(s) 28
BauI CACGAG 1 cut(s) 893
BbrPI CACGTG 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 4 cut(s) 279, 322, 325, 717
BccI CCATC 3 cut(s) 260, 761, 1034
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 1 cut(s) 318
BciVI GTATCC 2 cut(s) 811, 940
BcoDI GTCTC 2 cut(s) 233, 938
BfaI CTAG 3 cut(s) 149, 185, 396
BfuI GTATCC 2 cut(s) 811, 940
BisI GCNGC 5 cut(s) 162, 293, 311, 314, 706
BlnI CCTAGG 1 cut(s) 148
BlpI GCTNAGC 1 cut(s) 885
BlsI GCNGC 5 cut(s) 163, 294, 312, 315, 707
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 3 cut(s) 97, 622, 977
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 5 cut(s) 46, 304, 550, 640, 1072
BoxI GACNNNNGTC 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 885
Bpu14I TTCGAA 1 cut(s) 102
BsaAI YACGTR 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 35, 148, 879
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 4 cut(s) 241, 271, 445, 475
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 287
Bse118I RCCGGY 1 cut(s) 177
Bse3DI GCAATG 3 cut(s) 332, 796, 974
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 3 cut(s) 35, 148, 879
BseGI GGATG 3 cut(s) 252, 541, 1045
BseLI CCNNNNNNNGG 2 cut(s) 154, 287
BseMI GCAATG 3 cut(s) 332, 796, 974
BseMII CTCAG 2 cut(s) 244, 949
BseRI GAGGAG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 623
BseXI GCAGC 4 cut(s) 279, 322, 325, 717
BshFI GGCC 1 cut(s) 529
BshNI GGYRCC 1 cut(s) 620
BshTI ACCGGT 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 111
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 154, 287
BsmAI GTCTC 2 cut(s) 233, 938
BsmBI CGTCTC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 85
BsnI GGCC 1 cut(s) 529
Bsp119I TTCGAA 1 cut(s) 102
Bsp1286I GDGCHC 3 cut(s) 28, 111, 623
Bsp1407I TGTACA 1 cut(s) 344
Bsp143I GATC 7 cut(s) 223, 240, 277, 320, 502, 975, 1018
Bsp1720I GCTNAGC 1 cut(s) 885
Bsp19I CCATGG 1 cut(s) 35
BspACI CCGC 3 cut(s) 5, 28, 162
BspANI GGCC 1 cut(s) 529
BspCNI CTCAG 2 cut(s) 243, 948
BspLI GGNNCC 3 cut(s) 97, 622, 977
BspPI GGATC 6 cut(s) 272, 328, 510, 970, 983, 1013
BspQI GCTCTTC 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 102
BspT107I GGYRCC 1 cut(s) 620
BsrDI GCAATG 3 cut(s) 332, 796, 974
BsrFI RCCGGY 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 3 cut(s) 35, 148, 879
BssMI GATC 7 cut(s) 223, 240, 277, 320, 502, 975, 1018
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 893
BssT1I CCWWGG 3 cut(s) 35, 148, 879
Bst2BI CACGAG 1 cut(s) 893
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 4 cut(s) 211, 349, 731, 778
Bst6I CTCTTC 2 cut(s) 99, 397
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 2 cut(s) 56, 964
BstAUI TGTACA 1 cut(s) 344
BstBAI YACGTR 1 cut(s) 112
BstBI TTCGAA 1 cut(s) 102
BstC8I GCNNGC 3 cut(s) 28, 655, 787
BstDEI CTNAG 4 cut(s) 230, 338, 885, 935
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 3 cut(s) 252, 541, 1045
BstKTI GATC 7 cut(s) 226, 243, 280, 323, 505, 978, 1021
BstMAI GTCTC 2 cut(s) 233, 938
BstMBI GATC 7 cut(s) 223, 240, 277, 320, 502, 975, 1018
BstMWI GCNNNNNNNGC 5 cut(s) 56, 746, 895, 936, 964
BstNI CCWGG 1 cut(s) 318
BstPAI GACNNNNGTC 1 cut(s) 930
BstSCI CCNGG 1 cut(s) 316
BstSLI GKGCMC 1 cut(s) 623
BstV1I GCAGC 4 cut(s) 279, 322, 325, 717
BstX2I RGATCY 2 cut(s) 502, 975
BstYI RGATCY 2 cut(s) 502, 975
BsuI GTATCC 2 cut(s) 811, 940
BsuRI GGCC 1 cut(s) 529
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 3 cut(s) 252, 541, 1045
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 2 cut(s) 238, 735
Cac8I GCNNGC 3 cut(s) 28, 655, 787
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 245
CseI GACGC 3 cut(s) 55, 900, 974
Csp6I GTAC 1 cut(s) 345
CspAI ACCGGT 1 cut(s) 177
CspCI CAANNNNNGTGG 2 cut(s) 778, 813
CviAII CATG 1 cut(s) 36
CviQI GTAC 1 cut(s) 345
DdeI CTNAG 4 cut(s) 230, 338, 885, 935
DpnI GATC 7 cut(s) 225, 242, 279, 322, 504, 977, 1020
DpnII GATC 7 cut(s) 223, 240, 277, 320, 502, 975, 1018
EaeI YGGCCR 1 cut(s) 527
Eam1104I CTCTTC 2 cut(s) 99, 397
EarI CTCTTC 2 cut(s) 99, 397
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 35, 148, 879
Eco24I GRGCYC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 245
Eco72I CACGTG 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 35, 148, 879
EcoT22I ATGCAT 1 cut(s) 1087
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 3 cut(s) 35, 148, 879
Esp3I CGTCTC 1 cut(s) 233
FaeI CATG 1 cut(s) 39
FaqI GGGAC 1 cut(s) 140
FatI CATG 1 cut(s) 35
FauI CCCGC 1 cut(s) 35
Fnu4HI GCNGC 5 cut(s) 162, 293, 311, 314, 706
FokI GGATG 3 cut(s) 239, 528, 1052
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 5 cut(s) 162, 293, 311, 314, 706
FspBI CTAG 3 cut(s) 149, 185, 396
GluI GCNGC 5 cut(s) 162, 293, 311, 314, 706
HaeIII GGCC 1 cut(s) 529
HapII CCGG 1 cut(s) 178
HgaI GACGC 3 cut(s) 55, 900, 974
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 1 cut(s) 39
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 1 cut(s) 137
HpaII CCGG 1 cut(s) 178
HphI GGTGA 1 cut(s) 773
Hpy166II GTNNAC 4 cut(s) 385, 448, 458, 570
Hpy188I TCNGA 8 cut(s) 120, 136, 171, 476, 552, 975, 990, 1076
Hpy188III TCNNGA 4 cut(s) 141, 221, 506, 1089
Hpy8I GTNNAC 4 cut(s) 385, 448, 458, 570
HpyAV CCTTC 3 cut(s) 109, 655, 752
HpyCH4III ACNGT 4 cut(s) 211, 349, 731, 778
HpyCH4IV ACGT 2 cut(s) 111, 460
HpyCH4V TGCA 9 cut(s) 295, 362, 441, 684, 740, 755, 785, 967, 1085
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 746, 895, 936, 964
HpyF3I CTNAG 4 cut(s) 230, 338, 885, 935
HpySE526I ACGT 2 cut(s) 111, 460
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 1 cut(s) 39
Kzo9I GATC 7 cut(s) 223, 240, 277, 320, 502, 975, 1018
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 2 cut(s) 82, 95
LpnPI CCDG 8 cut(s) 75, 191, 303, 330, 620, 771, 813, 915
Lsp1109I GCAGC 4 cut(s) 279, 322, 325, 717
LweI GCATC 5 cut(s) 46, 304, 550, 640, 1072
MaeI CTAG 3 cut(s) 149, 185, 396
MaeII ACGT 2 cut(s) 111, 460
MaeIII GTNAC 4 cut(s) 180, 731, 1000, 1061
MalI GATC 7 cut(s) 225, 242, 279, 322, 504, 977, 1020
MboI GATC 7 cut(s) 223, 240, 277, 320, 502, 975, 1018
MboII GAAGA 5 cut(s) 116, 210, 384, 493, 776
MflI RGATCY 2 cut(s) 502, 975
MhlI GDGCHC 3 cut(s) 28, 111, 623
MlsI TGGCCA 1 cut(s) 529
MluCI AATT 1 cut(s) 546
MluNI TGGCCA 1 cut(s) 529
MmeI TCCRAC 3 cut(s) 272, 953, 1013
MnlI CCTC 8 cut(s) 175, 208, 267, 270, 470, 612, 948, 1037
Mox20I TGGCCA 1 cut(s) 529
Mph1103I ATGCAT 1 cut(s) 1087
MscI TGGCCA 1 cut(s) 529
MseI TTAA 1 cut(s) 674
MslI CAYNNNNRTG 1 cut(s) 90
Msp20I TGGCCA 1 cut(s) 529
MspA1I CMGCKG 2 cut(s) 708, 930
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 318
Mva1269I GAATGC 1 cut(s) 85
MvaI CCWGG 1 cut(s) 318
MwoI GCNNNNNNNGC 5 cut(s) 56, 746, 895, 936, 964
NcoI CCATGG 1 cut(s) 35
NdeII GATC 7 cut(s) 223, 240, 277, 320, 502, 975, 1018
NlaIII CATG 1 cut(s) 39
NlaIV GGNNCC 3 cut(s) 97, 622, 977
NmuCI GTSAC 4 cut(s) 180, 731, 1000, 1061
NsiI ATGCAT 1 cut(s) 1087
NspV TTCGAA 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 137
PinAI ACCGGT 1 cut(s) 177
PkrI GCNGC 5 cut(s) 163, 294, 312, 315, 707
PmaCI CACGTG 1 cut(s) 112
PmlI CACGTG 1 cut(s) 112
Ppu21I YACGTR 1 cut(s) 112
PshAI GACNNNNGTC 1 cut(s) 930
Psp6I CCWGG 1 cut(s) 316
PspCI CACGTG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 97, 622, 977
PspPI GGNCC 1 cut(s) 245
PsuI RGATCY 2 cut(s) 502, 975
PvuII CAGCTG 2 cut(s) 708, 930
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
RseI CAYNNNNRTG 1 cut(s) 90
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 1 cut(s) 674
SatI GCNGC 5 cut(s) 162, 293, 311, 314, 706
Sau3AI GATC 7 cut(s) 223, 240, 277, 320, 502, 975, 1018
Sau96I GGNCC 1 cut(s) 245
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 3 cut(s) 28, 111, 623
SfaNI GCATC 5 cut(s) 46, 304, 550, 640, 1072
SfuI TTCGAA 1 cut(s) 102
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 1 cut(s) 546
SsiI CCGC 3 cut(s) 5, 28, 162
SspMI CTAG 3 cut(s) 149, 185, 396
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 3 cut(s) 35, 148, 879
TaaI ACNGT 4 cut(s) 211, 349, 731, 778
TaiI ACGT 2 cut(s) 114, 463
TaqI TCGA 1 cut(s) 102
TaqII GACCGA 1 cut(s) 233
TasI AATT 1 cut(s) 546
TatI WGTACW 1 cut(s) 344
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 2 cut(s) 238, 742
TseFI GTSAC 4 cut(s) 180, 731, 1000, 1061
TseI GCWGC 4 cut(s) 292, 310, 313, 705
Tsp45I GTSAC 4 cut(s) 180, 731, 1000, 1061
TspDTI ATGAA 3 cut(s) 501, 506, 629
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 2 cut(s) 238, 742
VpaK11BI GGWCC 1 cut(s) 245
XmaJI CCTAGG 1 cut(s) 148
XspI CTAG 3 cut(s) 149, 185, 396
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.