RchiOBHm_Chr1g0349261

14 kDa proline-rich protein DC2.15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016483
Physical Location & Seq
Forward (+)
42373016 .. 42373563
548 bp
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UTR
Exon/CDS
Intron
PRQ57524

Sequence Viewer

Length: 384 bp
ATGGCTACTTCTTCTAAGGCAGTGGCAACTATCGCTCTTCTCCTCTCCCTCAACCTTGTGTTTTTCACAACAGTAAGTGCAACTCATCATGTCCCTTGCCCGCCGCCACCACCAGCAGCGGCGGCGACTTGTCCTAAGGACACCCTCAAGCTAGGGGTGTGTGCCAATCTGTTGAATGACCTAGTGCACCTTGTTGTGGGCACCCCACCCAAGACTCCCTGCTGTAGCCTCATTGAGGGTCTTGTTGATCTTGAAGCTGCTGTGTGCCTCTGCACTGCTATTAAGGCCAACATTCTGGGGATCAATCTGAACGTGCCAGTCTCTTTGAGCTTGCTTTTGAATTACTGTGGGAAGGGCGTCCCATCGGGCTTCCAATGCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

127

Amino Acids

12.96

Weight (kDa)

7.53

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 27 - 126 1.3e-07 Probable lipid transfer
Hydrophob_seed PF14547 43 - 127 2.2e-26 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 44 - 126 5.8e-17 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016483)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46890 AT5G46900
fragaria_vesca FvH4_7g11180
prunus_persica Prupe.2G139300_v2.0.a1 Prupe.2G139500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349261
rosa_multiflora Rmu_sc0000827.1_g000016 Rmu_sc0002914.1_g000026
rosa_roxburghii Rroxscaffold_4G00305740
rosa_rugosa Rorug01G0202200
rosa_samantha Rh1AG219800 Rh1BG185500 Rh1DG215200
rosa_wichuraiana Rw1G018650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 4 cut(s) 101, 104, 119, 122
AclWI GGATC 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 357
AfiI CCNNNNNNNGG 2 cut(s) 196, 235
AgsI TTSAA 3 cut(s) 175, 254, 340
AluBI AGCT 3 cut(s) 151, 257, 330
AluI AGCT 3 cut(s) 151, 257, 330
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 325
Alw44I GTGCAC 1 cut(s) 185
AlwI GGATC 1 cut(s) 308
AoxI GGCC 1 cut(s) 285
ApaLI GTGCAC 1 cut(s) 185
ApeKI GCWGC 2 cut(s) 116, 257
AspLEI GCGC 1 cut(s) 380
AxyI CCTNAGG 1 cut(s) 135
BaeGI GKGCMC 2 cut(s) 189, 203
BanI GGYRCC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 128, 244
BccI CCATC 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 325
BfaI CTAG 3 cut(s) 152, 182, 382
BfmI CTRYAG 1 cut(s) 223
BisI GCNGC 5 cut(s) 104, 117, 120, 123, 258
BlsI GCNGC 5 cut(s) 105, 118, 121, 124, 259
BmiI GGNNCC 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 357
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 235
Bse1I ACTGG 1 cut(s) 317
Bse21I CCTNAGG 1 cut(s) 135
BseLI CCNNNNNNNGG 2 cut(s) 196, 235
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 2 cut(s) 189, 203
BseXI GCAGC 2 cut(s) 128, 244
BsgI GTGCAG 1 cut(s) 256
BshFI GGCC 1 cut(s) 287
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 189
BslFI GGGAC 2 cut(s) 77, 344
BslI CCNNNNNNNGG 2 cut(s) 196, 235
BsmAI GTCTC 1 cut(s) 325
BsmFI GGGAC 2 cut(s) 77, 344
BsnI GGCC 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 189, 203
Bsp143I GATC 2 cut(s) 247, 300
BspACI CCGC 4 cut(s) 101, 104, 119, 122
BspANI GGCC 1 cut(s) 287
BspLI GGNNCC 1 cut(s) 202
BspPI GGATC 1 cut(s) 308
BspQI GCTCTTC 1 cut(s) 42
BspT107I GGYRCC 1 cut(s) 200
BsrI ACTGG 1 cut(s) 317
BssMI GATC 2 cut(s) 247, 300
BssNI GRCGYC 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 73, 347
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 357
BstC8I GCNNGC 2 cut(s) 101, 332
BstDEI CTNAG 2 cut(s) 15, 135
BstENI CCTNNNNNAGG 1 cut(s) 233
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 2 cut(s) 250, 303
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 2 cut(s) 247, 300
BstMWI GCNNNNNNNGC 4 cut(s) 32, 122, 284, 375
BstSFI CTRYAG 1 cut(s) 223
BstSLI GKGCMC 2 cut(s) 189, 203
BstV1I GCAGC 2 cut(s) 128, 244
BstXI CCANNNNNNTGG 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 135
BsuRI GGCC 1 cut(s) 287
BtsI GCAGTG 2 cut(s) 27, 273
BtsIMutI CAGTG 2 cut(s) 27, 273
Cac8I GCNNGC 2 cut(s) 101, 332
CfoI GCGC 1 cut(s) 380
CseI GACGC 1 cut(s) 346
CviAII CATG 1 cut(s) 89
CviJI RGCY 7 cut(s) 5, 151, 228, 257, 287, 330, 369
CviKI_1 RGCY 7 cut(s) 5, 151, 228, 257, 287, 330, 369
DdeI CTNAG 2 cut(s) 15, 135
DpnI GATC 2 cut(s) 249, 302
DpnII GATC 2 cut(s) 247, 300
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco81I CCTNAGG 1 cut(s) 135
EcoNI CCTNNNNNAGG 1 cut(s) 233
FaeI CATG 1 cut(s) 92
FaiI YATR 1 cut(s) 90
FaqI GGGAC 2 cut(s) 77, 344
FatI CATG 1 cut(s) 88
FauI CCCGC 1 cut(s) 108
Fnu4HI GCNGC 5 cut(s) 104, 117, 120, 123, 258
Fsp4HI GCNGC 5 cut(s) 104, 117, 120, 123, 258
FspBI CTAG 3 cut(s) 152, 182, 382
GlaI GCGC 1 cut(s) 379
GluI GCNGC 5 cut(s) 104, 117, 120, 123, 258
HaeIII GGCC 1 cut(s) 287
HgaI GACGC 1 cut(s) 346
HhaI GCGC 1 cut(s) 380
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 1 cut(s) 92
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HinfI GANTC 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 309
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 2 cut(s) 73, 347
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 3 cut(s) 80, 187, 273
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 122, 284, 375
HpyF3I CTNAG 2 cut(s) 15, 135
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 1 cut(s) 92
HspAI GCGC 1 cut(s) 378
Kzo9I GATC 2 cut(s) 247, 300
LguI GCTCTTC 1 cut(s) 42
LpnPI CCDG 4 cut(s) 126, 232, 281, 330
Lsp1109I GCAGC 2 cut(s) 128, 244
MaeI CTAG 3 cut(s) 152, 182, 382
MaeII ACGT 1 cut(s) 312
MalI GATC 2 cut(s) 249, 302
MboI GATC 2 cut(s) 247, 300
MboII GAAGA 2 cut(s) 3, 29
MhlI GDGCHC 2 cut(s) 189, 203
MluCI AATT 1 cut(s) 340
MlyI GAGTC 1 cut(s) 208
MnlI CCTC 6 cut(s) 53, 59, 155, 229, 239, 278
MseI TTAA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 119
MwoI GCNNNNNNNGC 4 cut(s) 32, 122, 284, 375
NdeII GATC 2 cut(s) 247, 300
NlaIII CATG 1 cut(s) 92
NlaIV GGNNCC 1 cut(s) 202
PciSI GCTCTTC 1 cut(s) 42
PkrI GCNGC 5 cut(s) 105, 118, 121, 124, 259
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 202
SapI GCTCTTC 1 cut(s) 42
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 5 cut(s) 104, 117, 120, 123, 258
Sau3AI GATC 2 cut(s) 247, 300
SchI GAGTC 1 cut(s) 208
SduI GDGCHC 2 cut(s) 189, 203
SetI ASST 7 cut(s) 57, 153, 183, 192, 259, 315, 332
SfcI CTRYAG 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 1 cut(s) 340
SsiI CCGC 4 cut(s) 101, 104, 119, 122
SspMI CTAG 3 cut(s) 152, 182, 382
TaaI ACNGT 2 cut(s) 73, 347
TaiI ACGT 1 cut(s) 315
TasI AATT 1 cut(s) 340
TauI GCSGC 3 cut(s) 106, 122, 125
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 2 cut(s) 27, 280
TseI GCWGC 2 cut(s) 116, 257
TspRI CASTG 2 cut(s) 27, 280
VneI GTGCAC 1 cut(s) 185
XagI CCTNNNNNAGG 1 cut(s) 233
XspI CTAG 3 cut(s) 152, 182, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.