RchiOBHm_Chr2g0106391

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017578
Physical Location & Seq
Reverse (-)
17806029 .. 17816196
10168 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48048

Sequence Viewer

Length: 1359 bp
ATGGAAAACCCCATCAAGCTCCTCCGATTTCATCCGATATCCCCAGTTGTAGGATGCAAGATTCATGCATCAGTTGATTTTGAGGATGATGATAGAGAATCTCCCGAATTGAAGCATGTTTGGTATCGAAAGCGGGAGAATGGAGAAAGGGAGCTGTTGGAGGTCACAGAGTTTTATACTCCCACGCCACAAGATGTTGGACTTAGCCTTGAGGTTGAAGTAATTGGGGCAGGTGTTTCAAACAATTTTTCTACGACCCCTGTGCTTGATTACCCTTTGATTCATCCTCGTCAGATGATTAGATATGGCTCATCAGCTGCAGTGGCTGATCTGCAAGAAATGGATACATTCACAGTGCTGACATATAATCTGATGACACATGTTCCTGGTTCTGCTTCGGAAGATCTTGGGTGGGCCGCATCGTGGCATTATCGGCAGAAGAGATTAGTAGATGAGATAACAGGATATCGTGCTGATATCCTTTGTCTTCAAGAAATCTATCAGAATGACTTCCGTACATGGTTTGAGCCGGAGCTACTAAAATTTGGATATGCAGGAATATTCAAGAAGAGATCTAATCCAAATTTGAAGGATGGATGTGCGACCTTTTATTTGAATAACCGATTTCGAAACCTAAATACATATGAGATTGAATTTGACACACTGGCAGATGAGGTTGCAATGAAATTTGGGACTCCACAGGAGACGGAGGAAGTTAAAATCCATCTTTCAACGGGGAATATAGCTGTGTGTGTCCTTCTAGAAAAAATTGATGGCTCTAATGGAAAACTTTGCGTGGCAAATACTCATATCTGCCCAATGTCTCATCAAAGAGGAGTATCCCTCTTTCAGATTGTCAGTTATGTACATGAGGTAGAAAAGTTTGCTGCCGATCCTAAGGTTCCTTTTATACTATGTGGAGATTTCAATGCTGATCCTGAAAGTGATGCTTATAAATTTGTAACTGAGGGGAAATTAGACTTGGAGGCACGTGACCGTAAATTCACTTATGTTCAAGTTAAGGGTTATTCTGATATAGAGAAGGCGGTATTCAAGCATTTGAAGCCGAAGCGTGGAACCTCCCTTGTAAGTGCTTACGCACATTTCTTCGGGTCTCTGCCTCCTGGTCATCCTTATCGTCAGAAAAAGCATGAAACATTGAATGAGCCTTGCTTCACATTTCTGAAATACCCCGTCGATAATGTGCTTGATTACATATTTTATACAGATGGGTCTCTACATCATGATGGAGTTCTTGAGCTTCCTAATCTCCGGAAGACTGATATTCTTCCTTCGATTCATTGGTCATCAGACCATATTGCCTTAATGGGGCGGTTTAGCTTTAAGGCGGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Exo_endo_phos zf-C6H2

Protein Analysis

452

Amino Acids

51.65

Weight (kDa)

5.71

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 127 - 439 5.1e-20 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017578)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16490
rosa_chinensis RchiOBHm_Chr2g0106371 RchiOBHm_Chr2g0106391
rosa_laevigata RLG00000017493 RLG00000017495
rosa_roxburghii Rroxscaffold_2G00137230
rosa_rugosa Rorug02G0135100
rosa_samantha Rh2AG186200 Rh2AG186300 Rh2CG191200 Rh2DG192300 Rh2DG192400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 954
AarI CACCTGC 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 221
AccIII TCCGGA 1 cut(s) 1272
AciI CCGC 5 cut(s) 133, 417, 1046, 1333, 1349
AclWI GGATC 2 cut(s) 887, 929
AcsI RAATTY 6 cut(s) 542, 583, 653, 686, 956, 1001
AcvI CACGTG 1 cut(s) 992
AfaI GTAC 2 cut(s) 517, 867
AfiI CCNNNNNNNGG 4 cut(s) 50, 423, 1073, 1329
AflIII ACRYGT 1 cut(s) 379
AjnI CCWGG 2 cut(s) 385, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
AluBI AGCT 7 cut(s) 19, 154, 317, 535, 746, 1261, 1341
AluI AGCT 7 cut(s) 19, 154, 317, 535, 746, 1261, 1341
Alw26I GTCTC 4 cut(s) 698, 828, 1119, 1239
AlwI GGATC 2 cut(s) 887, 929
AlwNI CAGNNNCTG 1 cut(s) 326
Aor13HI TCCGGA 1 cut(s) 1272
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 2 cut(s) 317, 887
ApoI RAATTY 6 cut(s) 542, 583, 653, 686, 956, 1001
ArsI GACNNNNNNTTYG 2 cut(s) 595, 627
Asp700I GAANNNNTTC 1 cut(s) 509
AspS9I GGNCC 1 cut(s) 414
AsuII TTCGAA 1 cut(s) 628
AxyI CCTNAGG 1 cut(s) 897
BbrPI CACGTG 1 cut(s) 992
BbsI GAAGAC 2 cut(s) 479, 1283
BbvI GCAGC 2 cut(s) 304, 874
BccI CCATC 6 cut(s) 20, 587, 732, 767, 1223, 1241
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 2 cut(s) 387, 1125
BciVI GTATCC 2 cut(s) 337, 850
BcoDI GTCTC 4 cut(s) 698, 828, 1119, 1239
BfaI CTAG 1 cut(s) 761
BfmI CTRYAG 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 221
BfuI GTATCC 2 cut(s) 337, 850
BglII AGATCT 2 cut(s) 403, 572
BisI GCNGC 3 cut(s) 318, 417, 888
BlsI GCNGC 3 cut(s) 319, 418, 889
Bme1390I CCNGG 2 cut(s) 387, 1125
BmgT120I GGNCC 1 cut(s) 414
BmiI GGNNCC 2 cut(s) 903, 1078
BmrFI CCNGG 2 cut(s) 387, 1125
BmrI ACTGGG 1 cut(s) 38
BmsI GCATC 4 cut(s) 44, 77, 428, 937
BmuI ACTGGG 1 cut(s) 38
BpiI GAAGAC 2 cut(s) 479, 1283
BplI GAGNNNNNCTC 2 cut(s) 828, 860
Bpu14I TTCGAA 1 cut(s) 628
BpuEI CTTGAG 2 cut(s) 230, 1277
BsaAI YACGTR 1 cut(s) 992
BsaI GGTCTC 2 cut(s) 1119, 1239
BsaWI WCCGGW 1 cut(s) 1272
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 423, 1073, 1329
Bse1I ACTGG 2 cut(s) 44, 669
Bse21I CCTNAGG 1 cut(s) 897
Bse3DI GCAATG 1 cut(s) 687
BseAI TCCGGA 1 cut(s) 1272
BseBI CCWGG 2 cut(s) 387, 1125
BseGI GGATG 7 cut(s) 31, 59, 91, 283, 598, 602, 1129
BseLI CCNNNNNNNGG 4 cut(s) 50, 423, 1073, 1329
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 1 cut(s) 957
BseNI ACTGG 2 cut(s) 44, 669
BseRI GAGGAG 2 cut(s) 11, 849
BseXI GCAGC 2 cut(s) 304, 874
BshFI GGCC 1 cut(s) 416
BsiSI CCGG 2 cut(s) 530, 1273
BslFI GGGAC 1 cut(s) 706
BslI CCNNNNNNNGG 4 cut(s) 50, 423, 1073, 1329
BsmAI GTCTC 4 cut(s) 698, 828, 1119, 1239
BsmBI CGTCTC 1 cut(s) 698
BsmFI GGGAC 1 cut(s) 706
BsnI GGCC 1 cut(s) 416
Bso31I GGTCTC 2 cut(s) 1119, 1239
Bsp119I TTCGAA 1 cut(s) 628
Bsp13I TCCGGA 1 cut(s) 1272
Bsp1407I TGTACA 1 cut(s) 865
Bsp143I GATC 5 cut(s) 328, 403, 572, 892, 934
BspACI CCGC 5 cut(s) 133, 417, 1046, 1333, 1349
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 1 cut(s) 958
BspEI TCCGGA 1 cut(s) 1272
BspHI TCATGA 1 cut(s) 1243
BspLI GGNNCC 2 cut(s) 903, 1078
BspMAI CTGCAG 1 cut(s) 322
BspMI ACCTGC 1 cut(s) 221
BspPI GGATC 2 cut(s) 887, 929
BspT104I TTCGAA 1 cut(s) 628
BspTNI GGTCTC 2 cut(s) 1119, 1239
BsrDI GCAATG 1 cut(s) 687
BsrGI TGTACA 1 cut(s) 865
BsrI ACTGG 2 cut(s) 44, 669
BssMI GATC 5 cut(s) 328, 403, 572, 892, 934
Bst2UI CCWGG 2 cut(s) 387, 1125
Bst4CI ACNGT 2 cut(s) 355, 998
Bst6I CTCTTC 2 cut(s) 434, 563
BstAUI TGTACA 1 cut(s) 865
BstBAI YACGTR 1 cut(s) 992
BstBI TTCGAA 1 cut(s) 628
BstDEI CTNAG 3 cut(s) 203, 897, 966
BstF5I GGATG 7 cut(s) 31, 59, 91, 283, 598, 602, 1129
BstKTI GATC 5 cut(s) 331, 406, 575, 895, 937
BstMAI GTCTC 4 cut(s) 698, 828, 1119, 1239
BstMBI GATC 5 cut(s) 328, 403, 572, 892, 934
BstMWI GCNNNNNNNGC 3 cut(s) 323, 433, 1063
BstNI CCWGG 2 cut(s) 387, 1125
BstNSI RCATGY 2 cut(s) 119, 383
BstSCI CCNGG 2 cut(s) 385, 1123
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 304, 874
BstV2I GAAGAC 2 cut(s) 479, 1283
BstX2I RGATCY 2 cut(s) 403, 572
BstYI RGATCY 2 cut(s) 403, 572
Bsu36I CCTNAGG 1 cut(s) 897
BsuI GTATCC 2 cut(s) 337, 850
BsuRI GGCC 1 cut(s) 416
BtsCI GGATG 7 cut(s) 31, 59, 91, 283, 598, 602, 1129
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 3 cut(s) 327, 360, 662
BveI ACCTGC 1 cut(s) 221
CaiI CAGNNNCTG 1 cut(s) 326
CciI TCATGA 1 cut(s) 1243
Cfr13I GGNCC 1 cut(s) 414
Csp6I GTAC 2 cut(s) 516, 866
CviAII CATG 7 cut(s) 65, 116, 380, 519, 869, 1151, 1244
CviQI GTAC 2 cut(s) 516, 866
DdeI CTNAG 3 cut(s) 203, 897, 966
DpnI GATC 5 cut(s) 330, 405, 574, 894, 936
DpnII GATC 5 cut(s) 328, 403, 572, 892, 934
Eam1104I CTCTTC 2 cut(s) 434, 563
EarI CTCTTC 2 cut(s) 434, 563
Eco31I GGTCTC 2 cut(s) 1119, 1239
Eco32I GATATC 3 cut(s) 39, 467, 478
Eco72I CACGTG 1 cut(s) 992
Eco81I CCTNAGG 1 cut(s) 897
EcoRII CCWGG 2 cut(s) 385, 1123
EcoRV GATATC 3 cut(s) 39, 467, 478
EcoT22I ATGCAT 1 cut(s) 70
Esp3I CGTCTC 1 cut(s) 698
FaeI CATG 7 cut(s) 68, 119, 383, 522, 872, 1154, 1247
FalI AAGNNNNNCTT 2 cut(s) 934, 966
FaqI GGGAC 1 cut(s) 706
FatI CATG 7 cut(s) 64, 115, 379, 518, 868, 1150, 1243
FauI CCCGC 1 cut(s) 126
FauNDI CATATG 1 cut(s) 643
Fnu4HI GCNGC 3 cut(s) 318, 417, 888
FokI GGATG 7 cut(s) 18, 66, 98, 270, 605, 609, 1116
Fsp4HI GCNGC 3 cut(s) 318, 417, 888
FspBI CTAG 1 cut(s) 761
GluI GCNGC 3 cut(s) 318, 417, 888
HaeIII GGCC 1 cut(s) 416
HapII CCGG 2 cut(s) 530, 1273
Hin1II CATG 7 cut(s) 68, 119, 383, 522, 872, 1154, 1247
HinfI GANTC 5 cut(s) 61, 98, 280, 694, 1297
HpaII CCGG 2 cut(s) 530, 1273
Hpy188III TCNNGA 8 cut(s) 104, 491, 565, 761, 938, 1244, 1256, 1273
Hpy99I CGWCG 1 cut(s) 1199
HpyAV CCTTC 4 cut(s) 583, 767, 1036, 1302
HpyCH4III ACNGT 2 cut(s) 355, 998
HpyCH4IV ACGT 1 cut(s) 991
HpyCH4V TGCA 6 cut(s) 57, 68, 320, 334, 554, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 433, 1063
HpyF3I CTNAG 3 cut(s) 203, 897, 966
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 7 cut(s) 68, 119, 383, 522, 872, 1154, 1247
Kpn2I TCCGGA 1 cut(s) 1272
Kzo9I GATC 5 cut(s) 328, 403, 572, 892, 934
LmnI GCTCC 3 cut(s) 24, 151, 532
Lsp1109I GCAGC 2 cut(s) 304, 874
LweI GCATC 4 cut(s) 44, 77, 428, 937
MaeI CTAG 1 cut(s) 761
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 3 cut(s) 163, 961, 992
MalI GATC 5 cut(s) 330, 405, 574, 894, 936
MboI GATC 5 cut(s) 328, 403, 572, 892, 934
MboII GAAGA 7 cut(s) 413, 451, 479, 580, 1099, 1280, 1288
MflI RGATCY 2 cut(s) 403, 572
MlyI GAGTC 1 cut(s) 688
MmeI TCCRAC 2 cut(s) 138, 178
Mph1103I ATGCAT 1 cut(s) 70
MroI TCCGGA 1 cut(s) 1272
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 4 cut(s) 717, 1020, 1325, 1344
MslI CAYNNNNRTG 1 cut(s) 1245
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 2 cut(s) 530, 1273
MspR9I CCNGG 2 cut(s) 387, 1125
MvaI CCWGG 2 cut(s) 387, 1125
MwoI GCNNNNNNNGC 3 cut(s) 323, 433, 1063
NdeI CATATG 1 cut(s) 643
NdeII GATC 5 cut(s) 328, 403, 572, 892, 934
NlaIII CATG 7 cut(s) 68, 119, 383, 522, 872, 1154, 1247
NlaIV GGNNCC 2 cut(s) 903, 1078
NmuCI GTSAC 2 cut(s) 163, 992
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 2 cut(s) 119, 383
NspV TTCGAA 1 cut(s) 628
PagI TCATGA 1 cut(s) 1243
PaqCI CACCTGC 1 cut(s) 221
PciI ACATGT 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 4 cut(s) 61, 98, 280, 1297
PkrI GCNGC 3 cut(s) 319, 418, 889
PleI GAGTC 1 cut(s) 688
PmaCI CACGTG 1 cut(s) 992
PmlI CACGTG 1 cut(s) 992
PpsI GAGTC 1 cut(s) 688
Ppu21I YACGTR 1 cut(s) 992
PscI ACATGT 1 cut(s) 379
PsiI TTATAA 1 cut(s) 954
Psp6I CCWGG 2 cut(s) 385, 1123
PspCI CACGTG 1 cut(s) 992
PspGI CCWGG 2 cut(s) 385, 1123
PspN4I GGNNCC 2 cut(s) 903, 1078
PspPI GGNCC 1 cut(s) 414
PstI CTGCAG 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 2 cut(s) 403, 572
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 2 cut(s) 517, 867
RsaNI GTAC 2 cut(s) 516, 866
RseI CAYNNNNRTG 1 cut(s) 1245
SaqAI TTAA 4 cut(s) 717, 1020, 1325, 1344
SatI GCNGC 3 cut(s) 318, 417, 888
Sau3AI GATC 5 cut(s) 328, 403, 572, 892, 934
Sau96I GGNCC 1 cut(s) 414
SchI GAGTC 1 cut(s) 688
ScrFI CCNGG 2 cut(s) 387, 1125
SfaNI GCATC 4 cut(s) 44, 77, 428, 937
SfcI CTRYAG 1 cut(s) 318
SfuI TTCGAA 1 cut(s) 628
SmiMI CAYNNNNRTG 1 cut(s) 1245
SmlI CTYRAG 2 cut(s) 209, 1256
SmoI CTYRAG 2 cut(s) 209, 1256
SsiI CCGC 5 cut(s) 133, 417, 1046, 1333, 1349
SspI AATATT 1 cut(s) 561
SspMI CTAG 1 cut(s) 761
StyD4I CCNGG 2 cut(s) 385, 1123
TaaI ACNGT 2 cut(s) 355, 998
TaiI ACGT 1 cut(s) 994
TaqI TCGA 4 cut(s) 127, 628, 1197, 1295
TatI WGTACW 1 cut(s) 865
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 4 cut(s) 61, 98, 280, 1297
Tru1I TTAA 4 cut(s) 717, 1020, 1325, 1344
Tru9I TTAA 4 cut(s) 717, 1020, 1325, 1344
TscAI CASTG 3 cut(s) 327, 360, 669
TseFI GTSAC 2 cut(s) 163, 992
TseI GCWGC 2 cut(s) 317, 887
Tsp45I GTSAC 2 cut(s) 163, 992
TspDTI ATGAA 6 cut(s) 20, 53, 272, 698, 1167, 1289
TspGWI ACGGA 2 cut(s) 503, 722
TspRI CASTG 3 cut(s) 327, 360, 669
XapI RAATTY 6 cut(s) 542, 583, 653, 686, 956, 1001
XbaI TCTAGA 1 cut(s) 760
XceI RCATGY 2 cut(s) 119, 383
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 1 cut(s) 761
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.