Rh2CG191200

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0017578
Physical Location & Seq
Reverse (-)
18027008 .. 18059886
32879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG191200.1

Sequence Viewer

Length: 3237 bp
ATGGAAAACCCCATCAAGCTCCTCCGATTTCATCCGATATCCCCAGTTGTAGGATGCAAGATTCATGCATCAGTTGATTTTGAGGATGATGATAGAGAATCTCCCGAATTGAAGCATGTTTGGTATCGAAAGCGGGAGAATGGAGAAAGGGAGCTGTTGGAGGTCACAGAGTTTTATACTCCCACGCCACAAGATGTTGGACTTAGCCTTGAGGTTGAAGTAATTGGGGCAGGTGTTTCAAACAATTTTTCTACGACCCCTGTGCTTGATTACCCTTTGATTCATCCTCGTCAGATGATTAGATATGGCTCATCAGCTGCAGTGGCTGATCTGCAAGAAATGGATACATTCACAGTGCTGACATATAATCTGATGACACATGTTCCTGGTTCTGCTTCGGAAGATCTTGGGTGGGCCGCATCGTGGCATTATCGGCAGAAGAGATTAGTAGATGAGATAACAGGATATCGTGCTGATATCCTTTGTCTTCAAGAAATCTATCAGAATGACTTCCGTACATGGTTTGAGCCGGAGCTACTAAAATTTGGATATGCAGGAATATTCAAGAAGAGATCTAATCCAAATTTGAAGGATGGATGTGCGACCTTTTATTTGAATAACCGATTTCGAAACCTAAATACATATGAGATTGAATTTGACACACTGGCAGATGAGGTTGCAATGAAATTTGGGACTCCACAGGAGACGGAGGAAGTTAAAATCCATCTTTCAACGGGGAATATAGCTGTGTGTGTCCTTCTAGAAAAAATTGATGGCTCTAATGGAAAACTTTGCGTGGCAAATACTCATATCTGCCCAATGTCTCATCAAAGAGGAGTATCCCTCTTTCAGATTGTCAGTTATGTACATGAGGTAGAAAAGTTTGCTGCCGATCCTAAGGTTCCTTTTATACTATGTGGAGATTTCAATGCTGATCCTGAAAGTGATGCTTATAAATTTGTAACTGAGGGGAAATTAGACTTGGAGGCACGTGACCGTAAATTCACTTATGTTCAAGTTAAGGGTTATTCTGATATAGAGAAGGCGGTATTCAAGCATTTGAAGCCGAAGCGTGGAACCTCCCTTGTAAGTGCTTACGCACATTTCTTCGGGTCTCTGCCTCCTGGTCATCCTTATCGTCAGAAAAAGCATGAAACATTGAATGAGCCTTGCTTCACATTTCTGAAATACCCCGTCGATAATGTGCTTGATTACATATTTTATACAGCTAGTAACCCTAGTCAGGAGGAAGGCGAGACGGCAGATCAGACTGGGAAGAAAAGTTGCACTCCACTGCAGGTTGAATCAGAGCCAGTTTCTCCTATTGGCAGCGTCGCTACAGATGCACAGCCCAAGAGTGCAAATACTCCATCTGTACTAAATCATGTTTTTGCTGGCTTTGACATGAATGCATTTTTGAAAAGCTGGAAGAGTCCAGTTTTGCCAAAACAGGATAGTTATCCAAAAGGGATGGAAGTTGGCGATGCAGCTAGGCACGTGGATTCGCATCACACTGCAAATTTGGGAAAGGGAGAGTTGGGAGTTGACGGAGGACTTGGAGATGGAGAAGCAGGAGAGGAAGGCGGAGGTGGACTCGAAGGAAGGGGAGGGGTCAGAGGAGTTGGTGGCGGCGAGGCGCGTGAGGGAGAACCTCCTGTTTCCAGTCCGATTCTGGTATTACTGCCAGCTGAAGATGTAGGTCAGAGTTTTCTTATAGGCGTCGGTTTCTCCTATTCTCAATCCATGGCTACATCACTCACTAAACTAATGGATCAGAAACAAGAATTTTCAAATTTGAAGGCTGCTAATGAGGACCGGATCAGTGAACTACCTGATTGTGTTCTATCCTATATTCTGTGTAAGTTGGCGATGCGAGATATGATGAAAACCCGTGTGCTGTCAAAAAGATGGCAAGGGCTCTGGGCGTTGAGGAGAGATCTTACTTTTGATTTTTCCCACATGTGCGATGACGTTGTTAAAATTCTTGAGAATGTCAGGTTCAGGAATGACCTTGAATACTATGCCAGGATTAATGAGGACTATGTGAAACGAGTTGATGAATTCATGCAGACTTTTCCAGGAGAGAAGATAGATTTCTTAAGGCTGTTTTACTTTGGTGATACTACCTCTACGGATGAGTCCATTGTTGATAAATGTGTTAGACATGCCATTGCAAAGGGAGTTGAACAGATTGATATGATCTCTGCGTCAATGTTGCCGCATTTCAGGCGTAGGTTTCCTTTTTGGCTTTTTCCTGTATCTAACAAGGGAACTAAAGCTTATTCAACTTTGAAGCACTTGTATCTGCGTTATTTTGTTCTTGCACCACCTCCGGATTTTACTGGATTCCACAATTTAGAATCTCTTACATTGGAGTGCACAATAGTTACTGAAGATTTGTTTAAATATCTGCTCTCCGATCCTGTCACACCGCTAAGAGTGTTGCGCCTGATAGCCTGCCGGTTTGAATTTGATTTAGAGATTGTTAGTCCATCCCTTCACCATTTTGAGATGATCGAGTGTTTGCACCTAGGGACTCTTACAATTTCTGCCATTAAACTCACTGTTTTTGAATATCACTGCTTCTACAAGTATGAGAGGGACCTTTTCCTGAATGCTCCACAGTTGACGAACATGTACTTTCACAAATTGTCTGAGAATCAGATACCTGCTGCAATTTCACAATTCATTGCTACACAGGTTGAAAATCTTAACCTGCGTATAGTTACTGAAAAGGTAATGCCAAGAAGCATAACCCCATTTTGGAATCTCAAGCAATTGGAGCTGACTGTTGAGAGTGTTGATATTGAAGAAGGTTTAGATGTGTTGTTAGTTTTCTCCTTCCTCAAGGCTTCCCCGCTTCTGCAAAAGCTCAAATTTAGGCGTTGGTCATCTGCTTCTGACGTCTCTCAGAAGCCAAATGAAAGTGAAAGATGGACGTTACACCAGGAATTCAGCCATAATGAACTTCAAGAAGTTGAAATTCTTGGATGTGTAGGCAATTGGTGCGAAATTGAACTCGCTATTTACTTGATGCAAAAGTCACGGTGTCTTGATAAACTTGTCTTTAACCCCTCCCGAAGTCCCTATCCTGGAGGTGGGAAATGGGATAAAGCTATTGTTAGTGGCCTTGAAAGGCCAAAGTCATGCCAGCATTGGCTAGAGTCGGGCCGGGAAACAGTCCGGGAAAAGCTTCAAGATAAAGTTCCTGCAAGTGTAAAACTTCTCTTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Exo_endo_phos zf-C6H2

Protein Analysis

1078

Amino Acids

122.3

Weight (kDa)

5.67

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 127 - 410 9.6e-17 Endonuclease/Exonuclease/phosphatase family
LRR_At1g61320_AtMIF1 PF23622 702 - 1076 9.1e-30 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 717 - 842 1.4e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017578)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16490
rosa_chinensis RchiOBHm_Chr2g0106371 RchiOBHm_Chr2g0106391
rosa_laevigata RLG00000017493 RLG00000017495
rosa_roxburghii Rroxscaffold_2G00137230
rosa_rugosa Rorug02G0135100
rosa_samantha Rh2AG186200 Rh2AG186300 Rh2CG191200 Rh2DG192300 Rh2DG192400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 954
AarI CACCTGC 1 cut(s) 221
AatII GACGTC 1 cut(s) 2907
Acc36I ACCTGC 4 cut(s) 221, 1288, 2677, 2724
AccII CGCG 1 cut(s) 1639
AccIII TCCGGA 1 cut(s) 2332
AciI CCGC 8 cut(s) 133, 417, 1046, 1584, 1629, 2219, 2432, 2858
AclWI GGATC 5 cut(s) 887, 929, 1779, 1826, 2414
AcuI CTGAAG 2 cut(s) 1710, 2412
AcvI CACGTG 2 cut(s) 992, 1498
AcyI GRCGYC 2 cut(s) 1719, 2904
AfaI GTAC 4 cut(s) 517, 867, 1377, 2639
AfiI CCNNNNNNNGG 7 cut(s) 50, 423, 1073, 1243, 2763, 3092, 3098
AflII CTTAAG 1 cut(s) 2098
AflIII ACRYGT 3 cut(s) 379, 1959, 2634
AjnI CCWGG 6 cut(s) 385, 1123, 2024, 2077, 2946, 3091
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
Alw21I GWGCWC 1 cut(s) 2381
Alw26I GTCTC 5 cut(s) 698, 828, 1119, 1250, 2911
Alw44I GTGCAC 1 cut(s) 2377
AlwI GGATC 5 cut(s) 887, 929, 1779, 1826, 2414
AlwNI CAGNNNCTG 1 cut(s) 326
Aor13HI TCCGGA 1 cut(s) 2332
AoxI GGCC 4 cut(s) 414, 3127, 3137, 3169
ApaLI GTGCAC 1 cut(s) 2377
ApeKI GCWGC 6 cut(s) 317, 887, 1329, 1487, 1802, 2672
ArsI GACNNNNNNTTYG 2 cut(s) 595, 627
AseI ATTAAT 1 cut(s) 2031
Asp700I GAANNNNTTC 2 cut(s) 509, 3192
AspA2I CCTAGG 1 cut(s) 2530
AspLEI GCGC 2 cut(s) 1639, 2448
AspS9I GGNCC 4 cut(s) 414, 1813, 2602, 3169
AsuC2I CCSGG 2 cut(s) 3173, 3185
AsuHPI GGTGA 2 cut(s) 2129, 2492
AsuII TTCGAA 1 cut(s) 628
AvaII GGWCC 2 cut(s) 1813, 2602
AvrII CCTAGG 1 cut(s) 2530
AxyI CCTNAGG 1 cut(s) 897
BaeGI GKGCMC 1 cut(s) 2381
BanII GRGCYC 1 cut(s) 1920
BbrPI CACGTG 2 cut(s) 992, 1498
BbsI GAAGAC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 2381
BbvI GCAGC 6 cut(s) 304, 874, 1341, 1499, 1789, 2659
BceAI ACGGC 1 cut(s) 1275
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 6 cut(s) 387, 1125, 2026, 2079, 2948, 3093
BciVI GTATCC 2 cut(s) 337, 850
BcnI CCSGG 2 cut(s) 3173, 3185
BcoDI GTCTC 5 cut(s) 698, 828, 1119, 1250, 2911
BfaI CTAG 6 cut(s) 761, 1230, 1239, 1491, 2531, 3161
BfmI CTRYAG 3 cut(s) 318, 1295, 1338
BfrI CTTAAG 1 cut(s) 2098
BfuAI ACCTGC 4 cut(s) 221, 1288, 2677, 2724
BfuI GTATCC 2 cut(s) 337, 850
BglII AGATCT 3 cut(s) 403, 572, 1936
BisI GCNGC 9 cut(s) 318, 417, 888, 1330, 1488, 1630, 1803, 2219, 2673
BlnI CCTAGG 1 cut(s) 2530
BlsI GCNGC 9 cut(s) 319, 418, 889, 1331, 1489, 1631, 1804, 2220, 2674
Bme1390I CCNGG 8 cut(s) 387, 1125, 2026, 2079, 2948, 3093, 3173, 3185
Bme18I GGWCC 2 cut(s) 1813, 2602
BmgT120I GGNCC 4 cut(s) 414, 1813, 2602, 3169
BmiI GGNNCC 3 cut(s) 903, 1078, 2603
BmrFI CCNGG 8 cut(s) 387, 1125, 2026, 2079, 2948, 3093, 3173, 3185
BmrI ACTGGG 2 cut(s) 38, 1281
BmsI GCATC 9 cut(s) 44, 77, 428, 937, 1333, 1474, 1516, 1860, 3024
BmuI ACTGGG 2 cut(s) 38, 1281
BpiI GAAGAC 1 cut(s) 479
BplI GAGNNNNNCTC 4 cut(s) 828, 860, 1578, 1610
BpmI CTGGAG 1 cut(s) 3114
Bpu14I TTCGAA 1 cut(s) 628
BpuEI CTTGAG 4 cut(s) 230, 2006, 2756, 2831
BpuMI CCSGG 2 cut(s) 3173, 3185
BsaAI YACGTR 2 cut(s) 992, 1498
BsaBI GATNNNNATC 2 cut(s) 1458, 1506
BsaHI GRCGYC 2 cut(s) 1719, 2904
BsaI GGTCTC 1 cut(s) 1119
BsaJI CCNNGG 2 cut(s) 1743, 2530
BsaWI WCCGGW 2 cut(s) 1815, 2332
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 423, 1073, 1243, 2763, 3092, 3098
Bse118I RCCGGY 1 cut(s) 2460
Bse1I ACTGG 7 cut(s) 44, 669, 1276, 1313, 1436, 1662, 2347
Bse21I CCTNAGG 1 cut(s) 897
Bse3DI GCAATG 3 cut(s) 687, 2169, 2688
Bse8I GATNNNNATC 2 cut(s) 1458, 1506
BseAI TCCGGA 1 cut(s) 2332
BseBI CCWGG 6 cut(s) 387, 1125, 2026, 2079, 2948, 3093
BseDI CCNNGG 2 cut(s) 1743, 2530
BseJI GATNNNNATC 2 cut(s) 1458, 1506
BseLI CCNNNNNNNGG 7 cut(s) 50, 423, 1073, 1243, 2763, 3092, 3098
BseMI GCAATG 3 cut(s) 687, 2169, 2688
BseMII CTCAG 3 cut(s) 957, 2646, 2924
BseNI ACTGG 7 cut(s) 44, 669, 1276, 1313, 1436, 1662, 2347
BseRI GAGGAG 4 cut(s) 11, 849, 1632, 1945
BseSI GKGCMC 1 cut(s) 2381
BseXI GCAGC 6 cut(s) 304, 874, 1341, 1499, 1789, 2659
Bsh1236I CGCG 1 cut(s) 1639
BshFI GGCC 4 cut(s) 416, 3129, 3139, 3171
BsiHKAI GWGCWC 1 cut(s) 2381
BsiSI CCGG 6 cut(s) 530, 1816, 2333, 2461, 3172, 3184
BslFI GGGAC 4 cut(s) 706, 2548, 2615, 3069
BslI CCNNNNNNNGG 7 cut(s) 50, 423, 1073, 1243, 2763, 3092, 3098
BsmAI GTCTC 5 cut(s) 698, 828, 1119, 1250, 2911
BsmBI CGTCTC 3 cut(s) 698, 1250, 2911
BsmFI GGGAC 4 cut(s) 706, 2548, 2615, 3069
BsmI GAATGC 2 cut(s) 1414, 2620
BsnI GGCC 4 cut(s) 416, 3129, 3139, 3171
Bso31I GGTCTC 1 cut(s) 1119
Bsp119I TTCGAA 1 cut(s) 628
Bsp1286I GDGCHC 2 cut(s) 1920, 2381
Bsp13I TCCGGA 1 cut(s) 2332
Bsp1407I TGTACA 1 cut(s) 865
Bsp19I CCATGG 1 cut(s) 1743
BspACI CCGC 8 cut(s) 133, 417, 1046, 1584, 1629, 2219, 2432, 2858
BspANI GGCC 4 cut(s) 416, 3129, 3139, 3171
BspCNI CTCAG 3 cut(s) 958, 2647, 2923
BspEI TCCGGA 1 cut(s) 2332
BspFNI CGCG 1 cut(s) 1639
BspLI GGNNCC 3 cut(s) 903, 1078, 2603
BspMAI CTGCAG 2 cut(s) 322, 1299
BspMI ACCTGC 4 cut(s) 221, 1288, 2677, 2724
BspPI GGATC 5 cut(s) 887, 929, 1779, 1826, 2414
BspT104I TTCGAA 1 cut(s) 628
BspTI CTTAAG 1 cut(s) 2098
BspTNI GGTCTC 1 cut(s) 1119
BsrDI GCAATG 3 cut(s) 687, 2169, 2688
BsrFI RCCGGY 1 cut(s) 2460
BsrGI TGTACA 1 cut(s) 865
BsrI ACTGG 7 cut(s) 44, 669, 1276, 1313, 1436, 1662, 2347
BssAI RCCGGY 1 cut(s) 2460
BssECI CCNNGG 2 cut(s) 1743, 2530
BssNI GRCGYC 2 cut(s) 1719, 2904
BssT1I CCWWGG 2 cut(s) 1743, 2530
Bst2UI CCWGG 6 cut(s) 387, 1125, 2026, 2079, 2948, 3093
Bst4CI ACNGT 7 cut(s) 355, 998, 2566, 2625, 2791, 3048, 3181
Bst6I CTCTTC 3 cut(s) 434, 563, 1424
BstACI GRCGYC 2 cut(s) 1719, 2904
BstAFI CTTAAG 1 cut(s) 2098
BstAPI GCANNNNNTGC 1 cut(s) 3006
BstAUI TGTACA 1 cut(s) 865
BstBAI YACGTR 2 cut(s) 992, 1498
BstBI TTCGAA 1 cut(s) 628
BstC8I GCNNGC 4 cut(s) 1396, 1686, 2458, 3152
BstDEI CTNAG 6 cut(s) 203, 897, 966, 2435, 2655, 2910
BstDSI CCRYGG 1 cut(s) 1743
BstFNI CGCG 1 cut(s) 1639
BstHHI GCGC 2 cut(s) 1639, 2448
BstMAI GTCTC 5 cut(s) 698, 828, 1119, 1250, 2911
BstMWI GCNNNNNNNGC 7 cut(s) 323, 433, 1063, 1343, 2227, 2782, 3006
BstNI CCWGG 6 cut(s) 387, 1125, 2026, 2079, 2948, 3093
BstNSI RCATGY 5 cut(s) 119, 383, 1963, 2168, 2638
BstSCI CCNGG 8 cut(s) 385, 1123, 2024, 2077, 2946, 3091, 3171, 3183
BstSFI CTRYAG 3 cut(s) 318, 1295, 1338
BstSLI GKGCMC 1 cut(s) 2381
BstUI CGCG 1 cut(s) 1639
BstV1I GCAGC 6 cut(s) 304, 874, 1341, 1499, 1789, 2659
BstV2I GAAGAC 1 cut(s) 479
BstX2I RGATCY 3 cut(s) 403, 572, 1936
BstYI RGATCY 3 cut(s) 403, 572, 1936
Bsu36I CCTNAGG 1 cut(s) 897
BsuI GTATCC 2 cut(s) 337, 850
BsuRI GGCC 4 cut(s) 416, 3129, 3139, 3171
BtgI CCRYGG 1 cut(s) 1743
BtgZI GCGATG 3 cut(s) 1497, 1883, 1980
BtsI GCAGTG 4 cut(s) 327, 1292, 1512, 2578
BtsIMutI CAGTG 8 cut(s) 327, 360, 662, 1292, 1512, 1828, 2562, 2578
BveI ACCTGC 4 cut(s) 221, 1288, 2677, 2724
Cac8I GCNNGC 4 cut(s) 1396, 1686, 2458, 3152
CaiI CAGNNNCTG 1 cut(s) 326
CfoI GCGC 2 cut(s) 1639, 2448
Cfr10I RCCGGY 1 cut(s) 2460
Cfr13I GGNCC 4 cut(s) 414, 1813, 2602, 3169
CseI GACGC 3 cut(s) 1321, 1708, 2196
Csp6I GTAC 4 cut(s) 516, 866, 1376, 2638
CviQI GTAC 4 cut(s) 516, 866, 1376, 2638
DdeI CTNAG 6 cut(s) 203, 897, 966, 2435, 2655, 2910
DraI TTTAAA 1 cut(s) 2404
Eam1104I CTCTTC 3 cut(s) 434, 563, 1424
EarI CTCTTC 3 cut(s) 434, 563, 1424
EciI GGCGGA 1 cut(s) 1599
Eco130I CCWWGG 2 cut(s) 1743, 2530
Eco24I GRGCYC 1 cut(s) 1920
Eco31I GGTCTC 1 cut(s) 1119
Eco32I GATATC 3 cut(s) 39, 467, 478
Eco47I GGWCC 2 cut(s) 1813, 2602
Eco57I CTGAAG 2 cut(s) 1710, 2412
Eco72I CACGTG 2 cut(s) 992, 1498
Eco81I CCTNAGG 1 cut(s) 897
EcoO109I RGGNCCY 1 cut(s) 2602
EcoRI GAATTC 2 cut(s) 2060, 2951
EcoRII CCWGG 6 cut(s) 385, 1123, 2024, 2077, 2946, 3091
EcoRV GATATC 3 cut(s) 39, 467, 478
EcoT14I CCWWGG 2 cut(s) 1743, 2530
EcoT22I ATGCAT 2 cut(s) 70, 1414
EcoT38I GRGCYC 1 cut(s) 1920
ErhI CCWWGG 2 cut(s) 1743, 2530
Esp3I CGTCTC 3 cut(s) 698, 1250, 2911
FalI AAGNNNNNCTT 2 cut(s) 934, 966
FaqI GGGAC 4 cut(s) 706, 2548, 2615, 3069
FauI CCCGC 2 cut(s) 126, 2865
FauNDI CATATG 1 cut(s) 643
Fnu4HI GCNGC 9 cut(s) 318, 417, 888, 1330, 1488, 1630, 1803, 2219, 2673
FriOI GRGCYC 1 cut(s) 1920
Fsp4HI GCNGC 9 cut(s) 318, 417, 888, 1330, 1488, 1630, 1803, 2219, 2673
FspBI CTAG 6 cut(s) 761, 1230, 1239, 1491, 2531, 3161
GlaI GCGC 2 cut(s) 1638, 2447
GluI GCNGC 9 cut(s) 318, 417, 888, 1330, 1488, 1630, 1803, 2219, 2673
GsuI CTGGAG 1 cut(s) 3114
HaeIII GGCC 4 cut(s) 416, 3129, 3139, 3171
HapII CCGG 6 cut(s) 530, 1816, 2333, 2461, 3172, 3184
HgaI GACGC 3 cut(s) 1321, 1708, 2196
HhaI GCGC 2 cut(s) 1639, 2448
Hin1I GRCGYC 2 cut(s) 1719, 2904
Hin6I GCGC 2 cut(s) 1637, 2446
HinP1I GCGC 2 cut(s) 1637, 2446
HincII GTYRAC 2 cut(s) 1546, 2628
HindII GTYRAC 2 cut(s) 1546, 2628
HindIII AAGCTT 2 cut(s) 2277, 3191
HpaII CCGG 6 cut(s) 530, 1816, 2333, 2461, 3172, 3184
HphI GGTGA 2 cut(s) 2129, 2492
Hpy166II GTNNAC 5 cut(s) 1546, 1592, 1826, 2379, 2628
Hpy8I GTNNAC 5 cut(s) 1546, 1592, 1826, 2379, 2628
Hpy99I CGWCG 3 cut(s) 1199, 1337, 1724
HpyCH4III ACNGT 7 cut(s) 355, 998, 2566, 2625, 2791, 3048, 3181
HpyCH4IV ACGT 5 cut(s) 991, 1497, 1971, 2904, 2939
HpyF10VI GCNNNNNNNGC 7 cut(s) 323, 433, 1063, 1343, 2227, 2782, 3006
HpyF3I CTNAG 6 cut(s) 203, 897, 966, 2435, 2655, 2910
HpySE526I ACGT 5 cut(s) 991, 1497, 1971, 2904, 2939
Hsp92I GRCGYC 2 cut(s) 1719, 2904
HspAI GCGC 2 cut(s) 1637, 2446
Kpn2I TCCGGA 1 cut(s) 2332
LmnI GCTCC 5 cut(s) 24, 151, 532, 2623, 2782
Lsp1109I GCAGC 6 cut(s) 304, 874, 1341, 1499, 1789, 2659
LweI GCATC 9 cut(s) 44, 77, 428, 937, 1333, 1474, 1516, 1860, 3024
MaeI CTAG 6 cut(s) 761, 1230, 1239, 1491, 2531, 3161
MaeII ACGT 5 cut(s) 991, 1497, 1971, 2904, 2939
MaeIII GTNAC 9 cut(s) 163, 961, 992, 1232, 2386, 2425, 2725, 2940, 3042
MfeI CAATTG 2 cut(s) 2777, 3001
MflI RGATCY 3 cut(s) 403, 572, 1936
MhlI GDGCHC 2 cut(s) 1920, 2381
MlyI GAGTC 6 cut(s) 688, 1441, 1587, 2147, 2530, 3173
MmeI TCCRAC 2 cut(s) 138, 178
Mph1103I ATGCAT 2 cut(s) 70, 1414
MroI TCCGGA 1 cut(s) 2332
MroXI GAANNNNTTC 2 cut(s) 509, 3192
MseI TTAA 9 cut(s) 717, 1020, 1977, 2031, 2099, 2403, 2556, 2712, 3069
MslI CAYNNNNRTG 1 cut(s) 2374
MspA1I CMGCKG 2 cut(s) 317, 1688
MspCI CTTAAG 1 cut(s) 2098
MspI CCGG 6 cut(s) 530, 1816, 2333, 2461, 3172, 3184
MspR9I CCNGG 8 cut(s) 387, 1125, 2026, 2079, 2948, 3093, 3173, 3185
MunI CAATTG 2 cut(s) 2777, 3001
Mva1269I GAATGC 2 cut(s) 1414, 2620
MvaI CCWGG 6 cut(s) 387, 1125, 2026, 2079, 2948, 3093
MvnI CGCG 1 cut(s) 1639
MwoI GCNNNNNNNGC 7 cut(s) 323, 433, 1063, 1343, 2227, 2782, 3006
NciI CCSGG 2 cut(s) 3173, 3185
NcoI CCATGG 1 cut(s) 1743
NdeI CATATG 1 cut(s) 643
NlaIV GGNNCC 3 cut(s) 903, 1078, 2603
NmuCI GTSAC 4 cut(s) 163, 992, 2425, 3042
NsiI ATGCAT 2 cut(s) 70, 1414
NspI RCATGY 5 cut(s) 119, 383, 1963, 2168, 2638
NspV TTCGAA 1 cut(s) 628
PaqCI CACCTGC 1 cut(s) 221
PciI ACATGT 3 cut(s) 379, 1959, 2634
PctI GAATGC 2 cut(s) 1414, 2620
PdmI GAANNNNTTC 2 cut(s) 509, 3192
PfoI TCCNGGA 3 cut(s) 2077, 3091, 3183
PkrI GCNGC 9 cut(s) 319, 418, 889, 1331, 1489, 1631, 1804, 2220, 2674
PleI GAGTC 6 cut(s) 688, 1440, 1587, 2146, 2530, 3172
PmaCI CACGTG 2 cut(s) 992, 1498
PmlI CACGTG 2 cut(s) 992, 1498
PpsI GAGTC 6 cut(s) 688, 1440, 1587, 2146, 2530, 3172
Ppu21I YACGTR 2 cut(s) 992, 1498
PpuMI RGGWCCY 1 cut(s) 2602
PscI ACATGT 3 cut(s) 379, 1959, 2634
PshBI ATTAAT 1 cut(s) 2031
PsiI TTATAA 1 cut(s) 954
Psp5II RGGWCCY 1 cut(s) 2602
Psp6I CCWGG 6 cut(s) 385, 1123, 2024, 2077, 2946, 3091
PspCI CACGTG 2 cut(s) 992, 1498
PspGI CCWGG 6 cut(s) 385, 1123, 2024, 2077, 2946, 3091
PspN4I GGNNCC 3 cut(s) 903, 1078, 2603
PspPI GGNCC 4 cut(s) 414, 1813, 2602, 3169
PspPPI RGGWCCY 1 cut(s) 2602
PstI CTGCAG 2 cut(s) 322, 1299
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 3 cut(s) 403, 572, 1936
PvuII CAGCTG 2 cut(s) 317, 1688
RsaI GTAC 4 cut(s) 517, 867, 1377, 2639
RsaNI GTAC 4 cut(s) 516, 866, 1376, 2638
RseI CAYNNNNRTG 1 cut(s) 2374
SaqAI TTAA 9 cut(s) 717, 1020, 1977, 2031, 2099, 2403, 2556, 2712, 3069
SatI GCNGC 9 cut(s) 318, 417, 888, 1330, 1488, 1630, 1803, 2219, 2673
Sau96I GGNCC 4 cut(s) 414, 1813, 2602, 3169
SchI GAGTC 6 cut(s) 688, 1441, 1587, 2147, 2530, 3173
ScrFI CCNGG 8 cut(s) 387, 1125, 2026, 2079, 2948, 3093, 3173, 3185
SduI GDGCHC 2 cut(s) 1920, 2381
SfaNI GCATC 9 cut(s) 44, 77, 428, 937, 1333, 1474, 1516, 1860, 3024
SfcI CTRYAG 3 cut(s) 318, 1295, 1338
SfuI TTCGAA 1 cut(s) 628
SinI GGWCC 2 cut(s) 1813, 2602
SmiMI CAYNNNNRTG 1 cut(s) 2374
SmlI CTYRAG 5 cut(s) 209, 1985, 2098, 2771, 2846
SmoI CTYRAG 5 cut(s) 209, 1985, 2098, 2771, 2846
SsiI CCGC 8 cut(s) 133, 417, 1046, 1584, 1629, 2219, 2432, 2858
SspI AATATT 1 cut(s) 561
SspMI CTAG 6 cut(s) 761, 1230, 1239, 1491, 2531, 3161
StyD4I CCNGG 8 cut(s) 385, 1123, 2024, 2077, 2946, 3091, 3171, 3183
StyI CCWWGG 2 cut(s) 1743, 2530
TaaI ACNGT 7 cut(s) 355, 998, 2566, 2625, 2791, 3048, 3181
TaiI ACGT 5 cut(s) 994, 1500, 1974, 2907, 2942
TaqI TCGA 5 cut(s) 127, 628, 1197, 1596, 2517
TatI WGTACW 3 cut(s) 865, 1375, 2637
TauI GCSGC 3 cut(s) 419, 1632, 2221
Tru1I TTAA 9 cut(s) 717, 1020, 1977, 2031, 2099, 2403, 2556, 2712, 3069
Tru9I TTAA 9 cut(s) 717, 1020, 1977, 2031, 2099, 2403, 2556, 2712, 3069
TscAI CASTG 8 cut(s) 327, 360, 669, 1299, 1519, 1828, 2569, 2585
TseFI GTSAC 4 cut(s) 163, 992, 2425, 3042
TseI GCWGC 6 cut(s) 317, 887, 1329, 1487, 1802, 2672
Tsp45I GTSAC 4 cut(s) 163, 992, 2425, 3042
TspGWI ACGGA 4 cut(s) 503, 722, 1563, 2147
TspRI CASTG 8 cut(s) 327, 360, 669, 1299, 1519, 1828, 2569, 2585
Vha464I CTTAAG 1 cut(s) 2098
VneI GTGCAC 1 cut(s) 2377
VpaK11BI GGWCC 2 cut(s) 1813, 2602
VspI ATTAAT 1 cut(s) 2031
XbaI TCTAGA 1 cut(s) 760
XceI RCATGY 5 cut(s) 119, 383, 1963, 2168, 2638
XcmI CCANNNNNNNNNTGG 1 cut(s) 1669
XmaJI CCTAGG 1 cut(s) 2530
XmnI GAANNNNTTC 2 cut(s) 509, 3192
XspI CTAG 6 cut(s) 761, 1230, 1239, 1491, 2531, 3161
ZraI GACGTC 1 cut(s) 2905
Zsp2I ATGCAT 2 cut(s) 70, 1414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.