Rh2AG186300

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017578
Physical Location & Seq
Reverse (-)
18170164 .. 18200490
30327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG186300.1

Sequence Viewer

Length: 732 bp
ATGATCAGATATGGCTCATCAGCTGCAGTGGCTGATCTGCAAGAAATGGATACATTCACAGTGCTGACATATAATCTGATGACACATGTTCCTGGTTCTGCTTCGGAAGATCTTGGGTGGGCCGCATCGTGGCATTATCGGCAGAAGAGATTAGTAGGTGAGATAACAGGATATCGTGCTGATATCCTTTGTTTTCAAGAAATCTATCAGAATGACTTCCGTACATGGTTTGAGCCGGAGCTACTGAAATTTGGATATGCAGGAATATTCAAGAAGAGATCTAATCCTAATTTGAAGGATGGATGTGCGACCTTTTATTTGAATAACCGATTTCGAAACCTAAATACATATGAGATTGAATTTGACACACTGGCAGATGAGGTTGCAATGAAATTTGGGACTCCACAGGAGACGGAGGAAGTTAAAATCCATCTTTCAACGGGGAATATAGCTGTGTGTGTCCTTCTAGAAAAAATTGATGGCTCTAATGGAAAACTTTGCGTGGCAAATACTCATATCTGCCCAATGTCTCATCAAAGAGGAGTATCCCTCTTTCAGATTGTCAGTTATGTACATGAGGTAGAAAAGTTTGCTGCCGATCCTAAGGTTCCTTTTATACTATGTGGAGATTTCAATGCTGATCCTGAAAGTGATGCTTATAAATTTATTGTTCTTCTCTGGATTCCTCACTTTGTTTTCTTTTTAGTTGGCCCATTCCTCGAACTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Exo_endo_phos zf-C6H2

Protein Analysis

243

Amino Acids

27.74

Weight (kDa)

5.25

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 24 - 222 6.8e-15 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017578)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16490
rosa_chinensis RchiOBHm_Chr2g0106371 RchiOBHm_Chr2g0106391
rosa_laevigata RLG00000017493 RLG00000017495
rosa_roxburghii Rroxscaffold_2G00137230
rosa_rugosa Rorug02G0135100
rosa_samantha Rh2AG186200 Rh2AG186300 Rh2CG191200 Rh2DG192300 Rh2DG192400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 660
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 593, 635
AcsI RAATTY 4 cut(s) 248, 359, 392, 662
AfaI GTAC 2 cut(s) 223, 573
AfiI CCNNNNNNNGG 1 cut(s) 129
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 7 cut(s) 197, 271, 295, 322, 359, 438, 634
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 3 cut(s) 23, 241, 452
AluI AGCT 3 cut(s) 23, 241, 452
Alw26I GTCTC 2 cut(s) 404, 534
AlwI GGATC 2 cut(s) 593, 635
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 2 cut(s) 120, 709
ApeKI GCWGC 2 cut(s) 23, 593
ApoI RAATTY 4 cut(s) 248, 359, 392, 662
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
Asp700I GAANNNNTTC 1 cut(s) 215
AspS9I GGNCC 2 cut(s) 120, 710
AsuHPI GGTGA 1 cut(s) 170
AsuII TTCGAA 1 cut(s) 334
AxyI CCTNAGG 1 cut(s) 603
BbvI GCAGC 2 cut(s) 10, 580
BccI CCATC 3 cut(s) 293, 438, 473
BciT130I CCWGG 1 cut(s) 93
BciVI GTATCC 2 cut(s) 43, 556
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 404, 534
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 1 cut(s) 24
BfuI GTATCC 2 cut(s) 43, 556
BglII AGATCT 2 cut(s) 109, 278
BisI GCNGC 3 cut(s) 24, 123, 594
BlsI GCNGC 3 cut(s) 25, 124, 595
Bme1390I CCNGG 1 cut(s) 93
BmgT120I GGNCC 2 cut(s) 120, 710
BmiI GGNNCC 1 cut(s) 609
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 134, 643
BplI GAGNNNNNCTC 2 cut(s) 534, 566
Bpu14I TTCGAA 1 cut(s) 334
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 375
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 304, 308
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMI GCAATG 1 cut(s) 393
BseNI ACTGG 1 cut(s) 375
BseRI GAGGAG 1 cut(s) 555
BseXI GCAGC 2 cut(s) 10, 580
BshFI GGCC 2 cut(s) 122, 711
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 2 cut(s) 404, 534
BsmBI CGTCTC 1 cut(s) 404
BsmFI GGGAC 1 cut(s) 412
BsnI GGCC 2 cut(s) 122, 711
Bsp119I TTCGAA 1 cut(s) 334
Bsp1407I TGTACA 1 cut(s) 571
Bsp143I GATC 6 cut(s) 3, 34, 109, 278, 598, 640
BspACI CCGC 1 cut(s) 123
BspANI GGCC 2 cut(s) 122, 711
BspLI GGNNCC 1 cut(s) 609
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 2 cut(s) 593, 635
BspT104I TTCGAA 1 cut(s) 334
BsrDI GCAATG 1 cut(s) 393
BsrGI TGTACA 1 cut(s) 571
BsrI ACTGG 1 cut(s) 375
BssMI GATC 6 cut(s) 3, 34, 109, 278, 598, 640
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 1 cut(s) 61
Bst6I CTCTTC 2 cut(s) 140, 269
BstAUI TGTACA 1 cut(s) 571
BstBI TTCGAA 1 cut(s) 334
BstDEI CTNAG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 304, 308
BstKTI GATC 6 cut(s) 6, 37, 112, 281, 601, 643
BstMAI GTCTC 2 cut(s) 404, 534
BstMBI GATC 6 cut(s) 3, 34, 109, 278, 598, 640
BstMWI GCNNNNNNNGC 2 cut(s) 29, 139
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 10, 580
BstX2I RGATCY 2 cut(s) 109, 278
BstYI RGATCY 2 cut(s) 109, 278
Bsu36I CCTNAGG 1 cut(s) 603
BsuI GTATCC 2 cut(s) 43, 556
BsuRI GGCC 2 cut(s) 122, 711
BtsCI GGATG 2 cut(s) 304, 308
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 3 cut(s) 33, 66, 368
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 120, 710
Csp6I GTAC 2 cut(s) 222, 572
CviAII CATG 3 cut(s) 86, 225, 575
CviJI RGCY 9 cut(s) 15, 23, 32, 122, 235, 241, 452, 483, 711
CviKI_1 RGCY 9 cut(s) 15, 23, 32, 122, 235, 241, 452, 483, 711
CviQI GTAC 2 cut(s) 222, 572
DdeI CTNAG 1 cut(s) 603
DpnI GATC 6 cut(s) 5, 36, 111, 280, 600, 642
DpnII GATC 6 cut(s) 3, 34, 109, 278, 598, 640
Eam1104I CTCTTC 2 cut(s) 140, 269
EarI CTCTTC 2 cut(s) 140, 269
Eco32I GATATC 2 cut(s) 173, 184
Eco81I CCTNAGG 1 cut(s) 603
EcoRII CCWGG 1 cut(s) 91
EcoRV GATATC 2 cut(s) 173, 184
Esp3I CGTCTC 1 cut(s) 404
FaeI CATG 3 cut(s) 89, 228, 578
FalI AAGNNNNNCTT 2 cut(s) 640, 672
FaqI GGGAC 1 cut(s) 412
FatI CATG 3 cut(s) 85, 224, 574
FauNDI CATATG 1 cut(s) 349
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 24, 123, 594
FokI GGATG 2 cut(s) 311, 315
Fsp4HI GCNGC 3 cut(s) 24, 123, 594
FspBI CTAG 1 cut(s) 467
GluI GCNGC 3 cut(s) 24, 123, 594
HaeIII GGCC 2 cut(s) 122, 711
HapII CCGG 1 cut(s) 236
Hin1II CATG 3 cut(s) 89, 228, 578
HinfI GANTC 2 cut(s) 400, 682
HpaII CCGG 1 cut(s) 236
HphI GGTGA 1 cut(s) 170
Hpy188I TCNGA 5 cut(s) 8, 78, 106, 210, 558
Hpy188III TCNNGA 5 cut(s) 197, 271, 467, 644, 679
HpyAV CCTTC 2 cut(s) 289, 473
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4V TGCA 4 cut(s) 26, 40, 260, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 139
HpyF3I CTNAG 1 cut(s) 603
Hsp92II CATG 3 cut(s) 89, 228, 578
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 34, 109, 278, 598, 640
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 9 cut(s) 78, 105, 153, 246, 249, 356, 392, 657, 664
Lsp1109I GCAGC 2 cut(s) 10, 580
LweI GCATC 2 cut(s) 134, 643
MaeI CTAG 1 cut(s) 467
MalI GATC 6 cut(s) 5, 36, 111, 280, 600, 642
MboI GATC 6 cut(s) 3, 34, 109, 278, 598, 640
MboII GAAGA 4 cut(s) 119, 157, 286, 665
MflI RGATCY 2 cut(s) 109, 278
MluCI AATT 6 cut(s) 248, 289, 359, 392, 474, 662
MlyI GAGTC 1 cut(s) 394
MnlI CCTC 7 cut(s) 373, 409, 533, 560, 571, 696, 728
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 2 cut(s) 423, 730
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 29, 139
NdeI CATATG 1 cut(s) 349
NdeII GATC 6 cut(s) 3, 34, 109, 278, 598, 640
NlaIII CATG 3 cut(s) 89, 228, 578
NlaIV GGNNCC 1 cut(s) 609
NspI RCATGY 1 cut(s) 89
NspV TTCGAA 1 cut(s) 334
PciI ACATGT 1 cut(s) 85
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 682
PkrI GCNGC 3 cut(s) 25, 124, 595
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
PscI ACATGT 1 cut(s) 85
PsiI TTATAA 1 cut(s) 660
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 609
PspPI GGNCC 2 cut(s) 120, 710
PstI CTGCAG 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 2 cut(s) 109, 278
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 2 cut(s) 223, 573
RsaNI GTAC 2 cut(s) 222, 572
SaqAI TTAA 2 cut(s) 423, 730
SatI GCNGC 3 cut(s) 24, 123, 594
Sau3AI GATC 6 cut(s) 3, 34, 109, 278, 598, 640
Sau96I GGNCC 2 cut(s) 120, 710
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 1 cut(s) 93
SetI ASST 9 cut(s) 25, 160, 243, 314, 342, 384, 454, 582, 609
SfaNI GCATC 2 cut(s) 134, 643
SfcI CTRYAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 334
Sse9I AATT 6 cut(s) 248, 289, 359, 392, 474, 662
SsiI CCGC 1 cut(s) 123
SspI AATATT 1 cut(s) 267
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 91
TaaI ACNGT 1 cut(s) 61
TaqI TCGA 2 cut(s) 334, 720
TasI AATT 6 cut(s) 248, 289, 359, 392, 474, 662
TatI WGTACW 1 cut(s) 571
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 2 cut(s) 423, 730
Tru9I TTAA 2 cut(s) 423, 730
TscAI CASTG 3 cut(s) 33, 66, 375
TseI GCWGC 2 cut(s) 23, 593
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 2 cut(s) 209, 428
TspRI CASTG 3 cut(s) 33, 66, 375
XapI RAATTY 4 cut(s) 248, 359, 392, 662
XbaI TCTAGA 1 cut(s) 466
XceI RCATGY 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.