RchiOBHm_Chr2g0117221

Iron-sulfur assembly protein IscA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017952
Physical Location & Seq
Forward (+)
29501363 .. 29504209
2847 bp
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UTR
Exon/CDS
Intron
PRQ49019

Sequence Viewer

Length: 531 bp
ATGGCGTTCTCTGCAATAACAACGCACTGCCCATCCTCTCTTCTTCGTCCGCCGGCGACGAACCCGTCGGTATCGTCTTCAGCTCGGAATTCAATTTCCTTCAGATTTTCGTCCACAAGTCTCAGCCGTGGAAGAAGACCCATTTCAATTCGATCCGTTTCAACTTCGGCTGCGCCGAAATCCGAGGGCATTGCACCTGCGATTTCACTTACGGAGAATGCGTTGAAGCATTTCAATAAGATGAGAGCCGAACGTAATGAGAATTTATGCTTGAGAATTGGTGTTAAACAGGGTGGATGCTCTGGCATGTCGTATACGATGGACTTCGAGAGCAAAGAAAATGCAAGGCCAGATGATTCCATCATGGAATATAATGGTTTTGAGATAGTTTGTGATCCAAAGAGTCTCCTCTTCTTGTATGGCATGCAATTGGACTACAGTGATGCTCTTATTGGTGGAGGCTTTTCTTTCAAGAACCCTAATGCCACACAAACATGTGGTTGCGGTAAATCTTTTGCCGCAGAGATGTAG

Protein Analysis

176

Amino Acids

19.0

Weight (kDa)

8.8

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 68 - 170 5.5e-22 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10500
fragaria_vesca FvH4_1g22881
malus_domestica MD15G1331200.v1.1
prunus_persica Prupe.6G169400_v2.0.a1 Prupe.6G169400_v2.0.a1
pyrus_communis pycom15g29400
rosa_chinensis RchiOBHm_Chr2g0117221
rosa_laevigata RLG00000018336
rosa_roxburghii Rroxscaffold_2G00126630
rosa_rugosa Rorug02G0211400
rosa_wichuraiana Rw2G021070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 205
AasI GACNNNNNNGTC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 205
AccI GTMKAC 1 cut(s) 314
AciI CCGC 3 cut(s) 50, 504, 519
AclWI GGATC 2 cut(s) 147, 389
AcsI RAATTY 2 cut(s) 88, 262
AcuI CTGAAG 2 cut(s) 63, 85
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 6 cut(s) 93, 147, 162, 226, 235, 472
AluBI AGCT 1 cut(s) 83
AluI AGCT 1 cut(s) 83
Alw26I GTCTC 2 cut(s) 125, 410
AlwI GGATC 2 cut(s) 147, 389
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 88, 262
Asp700I GAANNNNTTC 1 cut(s) 230
AspLEI GCGC 1 cut(s) 175
BbsI GAAGAC 2 cut(s) 69, 142
BbvI GCAGC 1 cut(s) 157
BccI CCATC 3 cut(s) 40, 313, 368
BceAI ACGGC 1 cut(s) 111
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BcoDI GTCTC 2 cut(s) 125, 410
BfmI CTRYAG 1 cut(s) 436
BfuAI ACCTGC 1 cut(s) 205
BisI GCNGC 2 cut(s) 171, 519
BlsI GCNGC 2 cut(s) 172, 520
BmsI GCATC 2 cut(s) 287, 433
BpiI GAAGAC 2 cut(s) 69, 142
BpuEI CTTGAG 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 127, 183
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bse118I RCCGGY 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 189
BseDI CCNNGG 2 cut(s) 127, 183
BseGI GGATG 2 cut(s) 32, 302
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 1 cut(s) 157
BshFI GGCC 1 cut(s) 349
BsiSI CCGG 1 cut(s) 53
BsmAI GTCTC 2 cut(s) 125, 410
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 1 cut(s) 349
Bsp143I GATC 2 cut(s) 152, 394
BspACI CCGC 3 cut(s) 50, 504, 519
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 2 cut(s) 147, 389
BsrDI GCAATG 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 52
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 2 cut(s) 127, 183
BssMI GATC 2 cut(s) 152, 394
BssNAI GTATAC 1 cut(s) 315
Bst1107I GTATAC 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 440
Bst6I CTCTTC 2 cut(s) 45, 416
BstC8I GCNNGC 2 cut(s) 54, 425
BstDEI CTNAG 1 cut(s) 122
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 2 cut(s) 32, 302
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 2 cut(s) 155, 397
BstMAI GTCTC 2 cut(s) 125, 410
BstMBI GATC 2 cut(s) 152, 394
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 3 cut(s) 310, 427, 498
BstSFI CTRYAG 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 2 cut(s) 69, 142
BstZ17I GTATAC 1 cut(s) 315
BsuRI GGCC 1 cut(s) 349
BtgI CCRYGG 1 cut(s) 127
BtsCI GGATG 2 cut(s) 32, 302
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 445
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 2 cut(s) 54, 425
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 52
CviAII CATG 4 cut(s) 307, 364, 424, 495
CviJI RGCY 6 cut(s) 83, 126, 170, 248, 349, 462
CviKI_1 RGCY 6 cut(s) 83, 126, 170, 248, 349, 462
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 154, 396
DpnII GATC 2 cut(s) 152, 394
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 45, 416
EarI CTCTTC 2 cut(s) 45, 416
EciI GGCGGA 1 cut(s) 39
Eco57I CTGAAG 2 cut(s) 63, 85
EcoRI GAATTC 1 cut(s) 88
FaeI CATG 4 cut(s) 310, 367, 427, 498
FaiI YATR 8 cut(s) 268, 308, 315, 365, 372, 420, 425, 496
FatI CATG 4 cut(s) 306, 363, 423, 494
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 2 cut(s) 171, 519
FokI GGATG 2 cut(s) 19, 309
Fsp4HI GCNGC 2 cut(s) 171, 519
GlaI GCGC 1 cut(s) 174
GluI GCNGC 2 cut(s) 171, 519
HaeIII GGCC 1 cut(s) 349
HapII CCGG 1 cut(s) 53
HhaI GCGC 1 cut(s) 175
Hin1II CATG 4 cut(s) 310, 367, 427, 498
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 2 cut(s) 356, 403
HpaII CCGG 1 cut(s) 53
Hpy166II GTNNAC 2 cut(s) 114, 315
Hpy188I TCNGA 3 cut(s) 87, 104, 184
Hpy188III TCNNGA 2 cut(s) 328, 472
Hpy8I GTNNAC 2 cut(s) 114, 315
Hpy99I CGWCG 2 cut(s) 61, 70
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 4 cut(s) 14, 194, 344, 427
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 4 cut(s) 310, 367, 427, 498
HspAI GCGC 1 cut(s) 173
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
Kzo9I GATC 2 cut(s) 152, 394
LpnPI CCDG 5 cut(s) 66, 210, 275, 288, 363
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 2 cut(s) 287, 433
MaeII ACGT 1 cut(s) 253
MalI GATC 2 cut(s) 154, 396
MboI GATC 2 cut(s) 152, 394
MboII GAAGA 6 cut(s) 32, 35, 69, 144, 147, 403
MfeI CAATTG 1 cut(s) 428
MluCI AATT 6 cut(s) 88, 93, 147, 262, 276, 428
MlyI GAGTC 1 cut(s) 412
MnlI CCTC 4 cut(s) 46, 178, 419, 452
MreI CGCCGGCG 1 cut(s) 52
MroNI GCCGGC 1 cut(s) 52
MroXI GAANNNNTTC 1 cut(s) 230
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 493
MspI CCGG 1 cut(s) 53
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 54
NdeII GATC 2 cut(s) 152, 394
NgoMIV GCCGGC 1 cut(s) 52
NlaIII CATG 4 cut(s) 310, 367, 427, 498
NspI RCATGY 3 cut(s) 310, 427, 498
PaeI GCATGC 1 cut(s) 427
PaqCI CACCTGC 1 cut(s) 205
PciI ACATGT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 2 cut(s) 173, 218
PctI GAATGC 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 356
PkrI GCNGC 2 cut(s) 172, 520
PleI GAGTC 1 cut(s) 411
PpsI GAGTC 1 cut(s) 411
PscI ACATGT 1 cut(s) 494
RseI CAYNNNNRTG 1 cut(s) 493
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 2 cut(s) 171, 519
Sau3AI GATC 2 cut(s) 152, 394
SchI GAGTC 1 cut(s) 412
SetI ASST 3 cut(s) 85, 199, 256
SfaNI GCATC 2 cut(s) 287, 433
SfcI CTRYAG 1 cut(s) 436
SgrAI CRCCGGYG 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 493
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
SphI GCATGC 1 cut(s) 427
Sse9I AATT 6 cut(s) 88, 93, 147, 262, 276, 428
SsiI CCGC 3 cut(s) 50, 504, 519
TaaI ACNGT 1 cut(s) 440
TaiI ACGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 151, 327
TasI AATT 6 cut(s) 88, 93, 147, 262, 276, 428
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 2 cut(s) 32, 445
TseI GCWGC 1 cut(s) 170
TspGWI ACGGA 2 cut(s) 145, 227
TspRI CASTG 2 cut(s) 32, 445
XapI RAATTY 2 cut(s) 88, 262
XceI RCATGY 3 cut(s) 310, 427, 498
XmiI GTMKAC 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.