RLG00000018336

Iron-sulfur assembly protein IscA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017952
Physical Location & Seq
Forward (+)
28461207 .. 28463762
2556 bp
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UTR
Exon/CDS
Intron
RLM00000018336

Sequence Viewer

Length: 531 bp
ATGGCGTTCTCTGCAATAACAACGCACTCCCCCTCCTCTCTTCTTCGTCCGCCGGCGACGAACCCGTCGGTATCGTCTTCAGCTCGGACCTCAATTTCCTTCAGATTTTCATCCACAAGTCTCAGCCGTGGAAGAAGACCCATTTCAATTCGATCCGTTTCAACTTCGGCTGCGCCGAAATCCGAGGGCATTGCACCTGCGATTTCACTTACGGAGAATGCGTTGAGGCATTTCAATAAGATGAGAGCCGAACGTAATGAGGATTTATGCTTGAGAATTGGTGTTAAACAGGGTGGATGCTCTGGCATGTCGTATACGATGGACTTCGAGAGCAAAGAAAATGCAAGGCCAGATGATTCCGTCATGGAATATAATGGTTTTGAGATAGTTTGTGATCCAAAGAGCCTTCTCTTCTTGTATGGCATGCAATTGGACTACAGTGATGCTCTTATTGGTGGAGGCTTTTCTTTCAAGAACCCTAATGCTACACAAACATGTGGTTGCGGTAAATCTTTTGCCGCAGAGATGTAG

Protein Analysis

177

Amino Acids

18.99

Weight (kDa)

8.65

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 68 - 170 3.6e-22 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10500
fragaria_vesca FvH4_1g22881
malus_domestica MD15G1331200.v1.1
prunus_persica Prupe.6G169400_v2.0.a1 Prupe.6G169400_v2.0.a1
pyrus_communis pycom15g29400
rosa_chinensis RchiOBHm_Chr2g0117221
rosa_laevigata RLG00000018336
rosa_roxburghii Rroxscaffold_2G00126630
rosa_rugosa Rorug02G0211400
rosa_wichuraiana Rw2G021070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 205
AasI GACNNNNNNGTC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 205
AccI GTMKAC 1 cut(s) 314
AciI CCGC 3 cut(s) 50, 504, 519
AclWI GGATC 2 cut(s) 147, 389
AcuI CTGAAG 2 cut(s) 63, 85
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 4 cut(s) 147, 162, 235, 472
AluBI AGCT 1 cut(s) 83
AluI AGCT 1 cut(s) 83
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 2 cut(s) 147, 389
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 87
AvaII GGWCC 1 cut(s) 87
BbsI GAAGAC 2 cut(s) 69, 142
BbvI GCAGC 1 cut(s) 157
BccI CCATC 1 cut(s) 313
BceAI ACGGC 1 cut(s) 111
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BcoDI GTCTC 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 436
BfuAI ACCTGC 1 cut(s) 205
BisI GCNGC 2 cut(s) 171, 519
BlsI GCNGC 2 cut(s) 172, 520
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 87
BmsI GCATC 2 cut(s) 287, 433
BpiI GAAGAC 2 cut(s) 69, 142
BpuEI CTTGAG 1 cut(s) 292
BsaBI GATNNNNATC 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 127, 183
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 206, 236
Bse118I RCCGGY 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 127, 183
BseGI GGATG 2 cut(s) 110, 302
BseJI GATNNNNATC 1 cut(s) 109
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 157
BshFI GGCC 1 cut(s) 349
BsiSI CCGG 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 125
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 1 cut(s) 349
Bsp143I GATC 2 cut(s) 152, 394
BspACI CCGC 3 cut(s) 50, 504, 519
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 2 cut(s) 147, 389
BsrDI GCAATG 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 52
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 2 cut(s) 127, 183
BssMI GATC 2 cut(s) 152, 394
BssNAI GTATAC 1 cut(s) 315
Bst1107I GTATAC 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 440
Bst6I CTCTTC 2 cut(s) 45, 416
BstC8I GCNNGC 2 cut(s) 54, 425
BstDEI CTNAG 1 cut(s) 122
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 2 cut(s) 110, 302
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 2 cut(s) 155, 397
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 2 cut(s) 152, 394
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 3 cut(s) 310, 427, 498
BstSFI CTRYAG 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 2 cut(s) 69, 142
BstZ17I GTATAC 1 cut(s) 315
BsuRI GGCC 1 cut(s) 349
BtgI CCRYGG 1 cut(s) 127
BtsCI GGATG 2 cut(s) 110, 302
BtsIMutI CAGTG 1 cut(s) 445
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 2 cut(s) 54, 425
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 87
CviAII CATG 4 cut(s) 307, 364, 424, 495
CviJI RGCY 7 cut(s) 83, 126, 170, 248, 349, 405, 462
CviKI_1 RGCY 7 cut(s) 83, 126, 170, 248, 349, 405, 462
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 154, 396
DpnII GATC 2 cut(s) 152, 394
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 45, 416
EarI CTCTTC 2 cut(s) 45, 416
EciI GGCGGA 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 63, 85
FaeI CATG 4 cut(s) 310, 367, 427, 498
FaiI YATR 8 cut(s) 268, 308, 315, 365, 372, 420, 425, 496
FatI CATG 4 cut(s) 306, 363, 423, 494
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 2 cut(s) 171, 519
FokI GGATG 2 cut(s) 97, 309
Fsp4HI GCNGC 2 cut(s) 171, 519
GlaI GCGC 1 cut(s) 174
GluI GCNGC 2 cut(s) 171, 519
HaeIII GGCC 1 cut(s) 349
HapII CCGG 1 cut(s) 53
HhaI GCGC 1 cut(s) 175
Hin1II CATG 4 cut(s) 310, 367, 427, 498
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 1 cut(s) 356
HpaII CCGG 1 cut(s) 53
Hpy166II GTNNAC 1 cut(s) 315
Hpy188I TCNGA 3 cut(s) 87, 104, 184
Hpy188III TCNNGA 2 cut(s) 328, 472
Hpy8I GTNNAC 1 cut(s) 315
Hpy99I CGWCG 2 cut(s) 61, 70
HpyAV CCTTC 2 cut(s) 109, 416
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 4 cut(s) 14, 194, 344, 427
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 4 cut(s) 310, 367, 427, 498
HspAI GCGC 1 cut(s) 173
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
Kzo9I GATC 2 cut(s) 152, 394
LpnPI CCDG 5 cut(s) 66, 210, 275, 288, 363
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 2 cut(s) 287, 433
MaeII ACGT 1 cut(s) 253
MalI GATC 2 cut(s) 154, 396
MboI GATC 2 cut(s) 152, 394
MboII GAAGA 6 cut(s) 32, 35, 69, 144, 147, 403
MfeI CAATTG 1 cut(s) 428
MluCI AATT 4 cut(s) 93, 147, 276, 428
MnlI CCTC 7 cut(s) 43, 46, 100, 178, 219, 253, 452
MreI CGCCGGCG 1 cut(s) 52
MroNI GCCGGC 1 cut(s) 52
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 493
MspI CCGG 1 cut(s) 53
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 54
NdeII GATC 2 cut(s) 152, 394
NgoMIV GCCGGC 1 cut(s) 52
NlaIII CATG 4 cut(s) 310, 367, 427, 498
NspI RCATGY 3 cut(s) 310, 427, 498
PaeI GCATGC 1 cut(s) 427
PaqCI CACCTGC 1 cut(s) 205
PciI ACATGT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 2 cut(s) 173, 218
PctI GAATGC 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 54
PfeI GAWTC 1 cut(s) 356
PkrI GCNGC 2 cut(s) 172, 520
PscI ACATGT 1 cut(s) 494
PspPI GGNCC 1 cut(s) 87
RseI CAYNNNNRTG 1 cut(s) 493
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 2 cut(s) 171, 519
Sau3AI GATC 2 cut(s) 152, 394
Sau96I GGNCC 1 cut(s) 87
SetI ASST 4 cut(s) 85, 92, 199, 256
SfaNI GCATC 2 cut(s) 287, 433
SfcI CTRYAG 1 cut(s) 436
SgrAI CRCCGGYG 1 cut(s) 52
SinI GGWCC 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 493
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
SphI GCATGC 1 cut(s) 427
Sse9I AATT 4 cut(s) 93, 147, 276, 428
SsiI CCGC 3 cut(s) 50, 504, 519
TaaI ACNGT 1 cut(s) 440
TaiI ACGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 151, 327
TasI AATT 4 cut(s) 93, 147, 276, 428
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 445
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 3 cut(s) 145, 227, 349
TspRI CASTG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 87
XceI RCATGY 3 cut(s) 310, 427, 498
XmiI GTMKAC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.