RchiOBHm_Chr2g0146941

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016537
Physical Location & Seq
Reverse (-)
64589356 .. 64590127
772 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51661

Sequence Viewer

Length: 474 bp
ATGAAGGTTTCCTTCACACTTTCCTCACCACACTCCTCAACTCACAAAACCTTTCATCAAAACCCTTCTCGGAGATCGATCAGCTTGATGGCGAAGACAGGGAAGCTGACGAAGCTCAAGTCGGCCATAAAGAGATGGCCCTCCTTCACCAAACTGGCTCGCACCACAAGCTCCATAGCCGCCACAAACGACGATTCTTCCTCCGACCAATACGTCGACAACGACAGGGCAGTGATCTCCAAGCAGCTTCACGCCGTCTACGTGGGGAAGTCGCGGCGGCGGTACATGGTGAGCTCCGACGTGGTGGAGCACCCCATAATTCAAGAGCTGGTGGACAAGTCATGTGAGGAAGAAGAAGGGGTTGTGGTGGTGACTTGCGAGGTCGTGCTTTTCGAGCACTTGCTGTGGATGCTTGAGAACGCCGAGACTCAGTTGGGTTCCATGGACGAGCTGGTTGAGTTCTACACTTGCTGA

Protein Analysis

157

Amino Acids

17.76

Weight (kDa)

6.59

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 39 - 138 4.4e-20 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72430
fragaria_vesca FvH4_6g35410
prunus_persica Prupe.3G035000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0146941
rosa_laevigata RLG00000020228
rosa_multiflora Rmu_sc0003317.1_g000007 Rmu_sc0003317.1_g000008
rosa_roxburghii Rroxscaffold_2G00099840
rosa_rugosa Rorug02G0397600
rosa_samantha Rh2AG454100 Rh2BG466800 Rh2CG441200 Rh2DG475700
rosa_wichuraiana Rw2G037080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccI GTMKAC 2 cut(s) 216, 258
AccII CGCG 1 cut(s) 274
AciI CCGC 4 cut(s) 180, 274, 277, 280
AcoI YGGCCR 1 cut(s) 123
AfaI GTAC 1 cut(s) 284
AgsI TTSAA 1 cut(s) 323
AjiI CACGTC 1 cut(s) 301
AluBI AGCT 8 cut(s) 84, 106, 115, 171, 247, 294, 328, 451
AluI AGCT 8 cut(s) 84, 106, 115, 171, 247, 294, 328, 451
Alw21I GWGCWC 3 cut(s) 296, 312, 399
Alw26I GTCTC 1 cut(s) 419
AoxI GGCC 2 cut(s) 123, 137
ApeKI GCWGC 1 cut(s) 244
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 4 cut(s) 18, 139, 301, 382
BanII GRGCYC 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 101
Bbv12I GWGCWC 3 cut(s) 296, 312, 399
BbvI GCAGC 1 cut(s) 256
BccI CCATC 2 cut(s) 82, 129
BceAI ACGGC 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 419
BisI GCNGC 4 cut(s) 180, 245, 275, 278
BlsI GCNGC 4 cut(s) 181, 246, 276, 279
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 138
BmiI GGNNCC 1 cut(s) 439
BmsI GCATC 1 cut(s) 399
BpiI GAAGAC 1 cut(s) 101
BpuEI CTTGAG 2 cut(s) 101, 434
Bsa29I ATCGAT 1 cut(s) 77
BsaAI YACGTR 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 441
Bse1I ACTGG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 1 cut(s) 159
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 256
Bsh1236I CGCG 1 cut(s) 274
BshFI GGCC 2 cut(s) 125, 139
BshVI ATCGAT 1 cut(s) 77
BsiHKAI GWGCWC 3 cut(s) 296, 312, 399
BsmAI GTCTC 1 cut(s) 419
BsnI GGCC 2 cut(s) 125, 139
Bsp1286I GDGCHC 3 cut(s) 296, 312, 399
Bsp143I GATC 3 cut(s) 74, 78, 234
Bsp19I CCATGG 1 cut(s) 441
BspACI CCGC 4 cut(s) 180, 274, 277, 280
BspANI GGCC 2 cut(s) 125, 139
BspCNI CTCAG 1 cut(s) 442
BspDI ATCGAT 1 cut(s) 77
BspFNI CGCG 1 cut(s) 274
BspLI GGNNCC 1 cut(s) 439
BsrI ACTGG 1 cut(s) 159
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 3 cut(s) 74, 78, 234
BssT1I CCWWGG 1 cut(s) 441
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 429
BstDSI CCRYGG 1 cut(s) 441
BstF5I GGATG 1 cut(s) 414
BstFNI CGCG 1 cut(s) 274
BstKTI GATC 3 cut(s) 77, 81, 237
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 3 cut(s) 74, 78, 234
BstMWI GCNNNNNNNGC 4 cut(s) 112, 168, 394, 409
BstUI CGCG 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 256
BstV2I GAAGAC 1 cut(s) 101
Bsu15I ATCGAT 1 cut(s) 77
BsuRI GGCC 2 cut(s) 125, 139
BsuTUI ATCGAT 1 cut(s) 77
BtgI CCRYGG 1 cut(s) 441
BtrI CACGTC 1 cut(s) 301
BtsCI GGATG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 138
ClaI ATCGAT 1 cut(s) 77
Csp6I GTAC 1 cut(s) 283
CviAII CATG 3 cut(s) 286, 342, 442
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 429
DpnI GATC 3 cut(s) 76, 80, 236
DpnII GATC 3 cut(s) 74, 78, 234
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 441
Eco24I GRGCYC 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 294
EcoICRI GAGCTC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 441
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 3 cut(s) 289, 345, 445
FaiI YATR 6 cut(s) 128, 176, 287, 317, 343, 443
FalI AAGNNNNNCTT 1 cut(s) 28
FatI CATG 3 cut(s) 285, 341, 441
FblI GTMKAC 2 cut(s) 216, 258
Fnu4HI GCNGC 4 cut(s) 180, 245, 275, 278
FokI GGATG 1 cut(s) 421
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 4 cut(s) 180, 245, 275, 278
GluI GCNGC 4 cut(s) 180, 245, 275, 278
HaeIII GGCC 2 cut(s) 125, 139
Hin1II CATG 3 cut(s) 289, 345, 445
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 2 cut(s) 194, 427
HphI GGTGA 4 cut(s) 18, 139, 301, 382
Hpy166II GTNNAC 3 cut(s) 217, 259, 334
Hpy188I TCNGA 3 cut(s) 72, 205, 298
Hpy188III TCNNGA 1 cut(s) 323
Hpy8I GTNNAC 3 cut(s) 217, 259, 334
Hpy99I CGWCG 3 cut(s) 194, 218, 302
HpyAV CCTTC 4 cut(s) 22, 75, 154, 350
HpyCH4IV ACGT 3 cut(s) 213, 261, 300
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 168, 394, 409
HpyF3I CTNAG 1 cut(s) 429
HpySE526I ACGT 3 cut(s) 213, 261, 300
Hsp92II CATG 3 cut(s) 289, 345, 445
Kzo9I GATC 3 cut(s) 74, 78, 234
LmnI GCTCC 3 cut(s) 176, 299, 307
LpnPI CCDG 5 cut(s) 84, 140, 211, 314, 437
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 1 cut(s) 399
MaeII ACGT 3 cut(s) 213, 261, 300
MaeIII GTNAC 1 cut(s) 370
MalI GATC 3 cut(s) 76, 80, 236
MboI GATC 3 cut(s) 74, 78, 234
MboII GAAGA 4 cut(s) 106, 189, 362, 365
MhlI GDGCHC 3 cut(s) 296, 312, 399
MluCI AATT 1 cut(s) 318
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 2 cut(s) 228, 321
MnlI CCTC 6 cut(s) 34, 46, 151, 211, 340, 373
MvnI CGCG 1 cut(s) 274
MwoI GCNNNNNNNGC 4 cut(s) 112, 168, 394, 409
NcoI CCATGG 1 cut(s) 441
NdeII GATC 3 cut(s) 74, 78, 234
NlaIII CATG 3 cut(s) 289, 345, 445
NlaIV GGNNCC 1 cut(s) 439
NmeAIII GCCGAG 1 cut(s) 448
NmuCI GTSAC 1 cut(s) 370
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 258, 390
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 4 cut(s) 181, 246, 276, 279
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
Ppu21I YACGTR 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 138
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SacI GAGCTC 1 cut(s) 296
SalI GTCGAC 1 cut(s) 215
SatI GCNGC 4 cut(s) 180, 245, 275, 278
Sau3AI GATC 3 cut(s) 74, 78, 234
Sau96I GGNCC 1 cut(s) 138
SchI GAGTC 1 cut(s) 421
SduI GDGCHC 3 cut(s) 296, 312, 399
SfaNI GCATC 1 cut(s) 399
SmlI CTYRAG 2 cut(s) 116, 413
SmoI CTYRAG 2 cut(s) 116, 413
Sse9I AATT 1 cut(s) 318
SsiI CCGC 4 cut(s) 180, 274, 277, 280
SstI GAGCTC 1 cut(s) 296
StyI CCWWGG 1 cut(s) 441
TaiI ACGT 3 cut(s) 216, 264, 303
TaqI TCGA 3 cut(s) 77, 216, 393
TasI AATT 1 cut(s) 318
TauI GCSGC 3 cut(s) 182, 277, 280
TfiI GAWTC 1 cut(s) 194
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 244
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 2 cut(s) 17, 44
TspRI CASTG 1 cut(s) 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XmiI GTMKAC 2 cut(s) 216, 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.