Rh2AG454100

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0016537
Physical Location & Seq
Reverse (-)
66824605 .. 66825787
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG454100.1

Sequence Viewer

Length: 384 bp
ATGGCGAAGACAGGGAAGCTGACGAAGCTCAAGTCGGCCATAAAGAGATGGCCCTCCTTCACCAAACTGGCTCGCACCACAAGCTCCATAGCCGCCACAAACGACGATTCGTCCTCCGACCAATACGTCGACAACGACAGGGCAGTGATCTCCAAGCAGCTTCACGCCGTCTACGTGGGGAAGTCGCGGCGGCGGTACATGGTGAGCTCCGACGTGGTGGAGCACCCCATAATTCAAGAGCTGGTGGACAAGTCATGTGAGGAAGAAGGGGTTGTGGTGGTGACTTGCGAGGTCGTGCTTTTCGAGCACTTGCTGTGGATGCTTGAGAACGCCGAGACTCAGTTGGGTTCCATGGACGAGCTGGTTGAGTTCTACACTTGCTGA

Protein Analysis

127

Amino Acids

14.35

Weight (kDa)

5.36

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 10 - 108 7.5e-20 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72430
fragaria_vesca FvH4_6g35410
prunus_persica Prupe.3G035000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0146941
rosa_laevigata RLG00000020228
rosa_multiflora Rmu_sc0003317.1_g000007 Rmu_sc0003317.1_g000008
rosa_roxburghii Rroxscaffold_2G00099840
rosa_rugosa Rorug02G0397600
rosa_samantha Rh2AG454100 Rh2BG466800 Rh2CG441200 Rh2DG475700
rosa_wichuraiana Rw2G037080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AccI GTMKAC 2 cut(s) 129, 171
AccII CGCG 1 cut(s) 187
AciI CCGC 4 cut(s) 93, 187, 190, 193
AcoI YGGCCR 1 cut(s) 36
AfaI GTAC 1 cut(s) 197
AgsI TTSAA 1 cut(s) 236
AhdI GACNNNNNGTC 1 cut(s) 109
AjiI CACGTC 1 cut(s) 214
AluBI AGCT 7 cut(s) 19, 28, 84, 160, 207, 241, 361
AluI AGCT 7 cut(s) 19, 28, 84, 160, 207, 241, 361
Alw21I GWGCWC 3 cut(s) 209, 225, 309
Alw26I GTCTC 1 cut(s) 329
AoxI GGCC 2 cut(s) 36, 50
ApeKI GCWGC 1 cut(s) 157
ArsI GACNNNNNNTTYG 2 cut(s) 17, 49
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 3 cut(s) 52, 214, 292
BanII GRGCYC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 3 cut(s) 209, 225, 309
BbvI GCAGC 1 cut(s) 169
BccI CCATC 1 cut(s) 42
BceAI ACGGC 1 cut(s) 152
BcoDI GTCTC 1 cut(s) 329
BisI GCNGC 4 cut(s) 93, 158, 188, 191
BlsI GCNGC 4 cut(s) 94, 159, 189, 192
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 349
BmsI GCATC 1 cut(s) 309
BpiI GAAGAC 1 cut(s) 14
BpuEI CTTGAG 2 cut(s) 14, 344
BsaAI YACGTR 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 351
Bse1I ACTGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 324
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 72
BseXI GCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 38, 52
BsiHKAI GWGCWC 3 cut(s) 209, 225, 309
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 2 cut(s) 38, 52
Bsp1286I GDGCHC 3 cut(s) 209, 225, 309
Bsp143I GATC 1 cut(s) 147
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 4 cut(s) 93, 187, 190, 193
BspANI GGCC 2 cut(s) 38, 52
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 349
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 187
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 1 cut(s) 147
BstMWI GCNNNNNNNGC 4 cut(s) 25, 81, 304, 319
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 169
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 2 cut(s) 38, 52
BtgI CCRYGG 1 cut(s) 351
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 1 cut(s) 324
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 196
CviAII CATG 3 cut(s) 199, 255, 352
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 339
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
DrdI GACNNNNNNGTC 1 cut(s) 125
DriI GACNNNNNGTC 1 cut(s) 109
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 3 cut(s) 202, 258, 355
FaiI YATR 6 cut(s) 41, 89, 200, 230, 256, 353
FatI CATG 3 cut(s) 198, 254, 351
FblI GTMKAC 2 cut(s) 129, 171
Fnu4HI GCNGC 4 cut(s) 93, 158, 188, 191
FokI GGATG 1 cut(s) 331
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 4 cut(s) 93, 158, 188, 191
GluI GCNGC 4 cut(s) 93, 158, 188, 191
HaeIII GGCC 2 cut(s) 38, 52
Hin1II CATG 3 cut(s) 202, 258, 355
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 2 cut(s) 107, 337
HphI GGTGA 3 cut(s) 52, 214, 292
Hpy166II GTNNAC 3 cut(s) 130, 172, 247
Hpy188I TCNGA 2 cut(s) 118, 211
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 3 cut(s) 130, 172, 247
Hpy99I CGWCG 3 cut(s) 107, 131, 215
HpyAV CCTTC 2 cut(s) 67, 260
HpyCH4IV ACGT 3 cut(s) 126, 174, 213
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 81, 304, 319
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 3 cut(s) 126, 174, 213
Hsp92II CATG 3 cut(s) 202, 258, 355
Kzo9I GATC 1 cut(s) 147
LmnI GCTCC 3 cut(s) 89, 212, 220
LpnPI CCDG 4 cut(s) 53, 124, 227, 347
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 1 cut(s) 309
MaeII ACGT 3 cut(s) 126, 174, 213
MaeIII GTNAC 1 cut(s) 280
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 2 cut(s) 19, 275
MhlI GDGCHC 3 cut(s) 209, 225, 309
MluCI AATT 1 cut(s) 231
MlyI GAGTC 1 cut(s) 331
MmeI TCCRAC 2 cut(s) 141, 234
MnlI CCTC 4 cut(s) 64, 124, 253, 283
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 4 cut(s) 25, 81, 304, 319
NcoI CCATGG 1 cut(s) 351
NdeII GATC 1 cut(s) 147
NlaIII CATG 3 cut(s) 202, 258, 355
NlaIV GGNNCC 1 cut(s) 349
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 280
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 171, 300
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 4 cut(s) 94, 159, 189, 192
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
Ppu21I YACGTR 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 349
PspPI GGNCC 1 cut(s) 51
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SacI GAGCTC 1 cut(s) 209
SalI GTCGAC 1 cut(s) 128
SatI GCNGC 4 cut(s) 93, 158, 188, 191
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 1 cut(s) 51
SchI GAGTC 1 cut(s) 331
SduI GDGCHC 3 cut(s) 209, 225, 309
SfaNI GCATC 1 cut(s) 309
SmlI CTYRAG 2 cut(s) 29, 323
SmoI CTYRAG 2 cut(s) 29, 323
Sse9I AATT 1 cut(s) 231
SsiI CCGC 4 cut(s) 93, 187, 190, 193
SstI GAGCTC 1 cut(s) 209
StyI CCWWGG 1 cut(s) 351
TaiI ACGT 3 cut(s) 129, 177, 216
TaqI TCGA 2 cut(s) 129, 303
TasI AATT 1 cut(s) 231
TauI GCSGC 3 cut(s) 95, 190, 193
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 1 cut(s) 157
Tsp45I GTSAC 1 cut(s) 280
TspRI CASTG 1 cut(s) 150
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XmiI GTMKAC 2 cut(s) 129, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.