RLG00000020228

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016537
Physical Location & Seq
Reverse (-)
62670978 .. 62671361
384 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020228

Sequence Viewer

Length: 384 bp
ATGGCGAAGACAGGGAAGCTGACGAAGCTCAAGTCGGCCATAAAGAGATGGCCCTCCTTCACCAAACTGGCTCGCACCACAAGCTCCATAGCCGCCGCAAACGACGATTCGTCCTCTGACCAATACGGCAACGACAGGGCAGTGATCTCCAAGCAGCTTCACGCCGTCTACGTGGGGAAGTCGCGGAGGCGGTACATGGTGAGCTCCGACGTGGTGGAGCACCCCATAATTCAAGAGCTGGTGGACAAGTCGTGTGAGGAAGAAGAAGGGGTTGTGGTGGTGACTTGCGAGGTCGTGCTTTTCGAGCACTTGCTGTGGATGCTTGAGAACGCCGAGACTCAGTTGGGTTCCATGGACGAGCTGGTTGAGTTCTACACTTGCTGA

Protein Analysis

128

Amino Acids

14.29

Weight (kDa)

5.37

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 10 - 108 2.3e-20 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72430
fragaria_vesca FvH4_6g35410
prunus_persica Prupe.3G035000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0146941
rosa_laevigata RLG00000020228
rosa_multiflora Rmu_sc0003317.1_g000007 Rmu_sc0003317.1_g000008
rosa_roxburghii Rroxscaffold_2G00099840
rosa_rugosa Rorug02G0397600
rosa_samantha Rh2AG454100 Rh2BG466800 Rh2CG441200 Rh2DG475700
rosa_wichuraiana Rw2G037080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 168
AccII CGCG 1 cut(s) 184
AciI CCGC 4 cut(s) 93, 96, 184, 190
AcoI YGGCCR 1 cut(s) 36
AfaI GTAC 1 cut(s) 194
AgsI TTSAA 1 cut(s) 233
AhdI GACNNNNNGTC 1 cut(s) 109
AjiI CACGTC 1 cut(s) 211
AluBI AGCT 7 cut(s) 19, 28, 84, 157, 204, 238, 361
AluI AGCT 7 cut(s) 19, 28, 84, 157, 204, 238, 361
Alw21I GWGCWC 3 cut(s) 206, 222, 309
Alw26I GTCTC 1 cut(s) 329
AoxI GGCC 2 cut(s) 36, 50
ApeKI GCWGC 1 cut(s) 154
ArsI GACNNNNNNTTYG 2 cut(s) 17, 49
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 3 cut(s) 52, 211, 292
BanII GRGCYC 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 3 cut(s) 206, 222, 309
BbvI GCAGC 1 cut(s) 166
BccI CCATC 1 cut(s) 42
BceAI ACGGC 2 cut(s) 142, 149
BcoDI GTCTC 1 cut(s) 329
BisI GCNGC 3 cut(s) 93, 96, 155
BlsI GCNGC 3 cut(s) 94, 97, 156
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 349
BmsI GCATC 1 cut(s) 309
BpiI GAAGAC 1 cut(s) 14
BpuEI CTTGAG 2 cut(s) 14, 344
BsaAI YACGTR 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 351
Bse1I ACTGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 324
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 72
BseXI GCAGC 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 184
BshFI GGCC 2 cut(s) 38, 52
BsiHKAI GWGCWC 3 cut(s) 206, 222, 309
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 2 cut(s) 38, 52
Bsp1286I GDGCHC 3 cut(s) 206, 222, 309
Bsp143I GATC 1 cut(s) 144
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 4 cut(s) 93, 96, 184, 190
BspANI GGCC 2 cut(s) 38, 52
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 349
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 172
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 184
BstKTI GATC 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 1 cut(s) 144
BstMWI GCNNNNNNNGC 4 cut(s) 25, 81, 304, 319
BstUI CGCG 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 166
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 2 cut(s) 38, 52
BtgI CCRYGG 1 cut(s) 351
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 1 cut(s) 324
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 193
CviAII CATG 2 cut(s) 196, 352
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 339
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
DriI GACNNNNNGTC 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 204
EcoICRI GAGCTC 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 2 cut(s) 199, 355
FaiI YATR 5 cut(s) 41, 89, 197, 227, 353
FatI CATG 2 cut(s) 195, 351
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 93, 96, 155
FokI GGATG 1 cut(s) 331
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 3 cut(s) 93, 96, 155
GluI GCNGC 3 cut(s) 93, 96, 155
HaeIII GGCC 2 cut(s) 38, 52
Hin1II CATG 2 cut(s) 199, 355
HinfI GANTC 2 cut(s) 107, 337
HphI GGTGA 3 cut(s) 52, 211, 292
Hpy166II GTNNAC 2 cut(s) 169, 244
Hpy188I TCNGA 2 cut(s) 118, 208
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 2 cut(s) 169, 244
Hpy99I CGWCG 2 cut(s) 107, 212
HpyAV CCTTC 2 cut(s) 67, 260
HpyCH4IV ACGT 2 cut(s) 171, 210
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 81, 304, 319
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 2 cut(s) 171, 210
Hsp92II CATG 2 cut(s) 199, 355
Kzo9I GATC 1 cut(s) 144
LmnI GCTCC 3 cut(s) 89, 209, 217
LpnPI CCDG 4 cut(s) 53, 121, 224, 347
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 1 cut(s) 309
MaeII ACGT 2 cut(s) 171, 210
MaeIII GTNAC 1 cut(s) 280
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MboII GAAGA 3 cut(s) 19, 272, 275
MhlI GDGCHC 3 cut(s) 206, 222, 309
MluCI AATT 1 cut(s) 228
MlyI GAGTC 1 cut(s) 331
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 5 cut(s) 64, 124, 180, 250, 283
MvnI CGCG 1 cut(s) 184
MwoI GCNNNNNNNGC 4 cut(s) 25, 81, 304, 319
NcoI CCATGG 1 cut(s) 351
NdeII GATC 1 cut(s) 144
NlaIII CATG 2 cut(s) 199, 355
NlaIV GGNNCC 1 cut(s) 349
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 280
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 300
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 94, 97, 156
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
Ppu21I YACGTR 1 cut(s) 172
Psp124BI GAGCTC 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 349
PspPI GGNCC 1 cut(s) 51
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SacI GAGCTC 1 cut(s) 206
SatI GCNGC 3 cut(s) 93, 96, 155
Sau3AI GATC 1 cut(s) 144
Sau96I GGNCC 1 cut(s) 51
SchI GAGTC 1 cut(s) 331
SduI GDGCHC 3 cut(s) 206, 222, 309
SfaNI GCATC 1 cut(s) 309
SmlI CTYRAG 2 cut(s) 29, 323
SmoI CTYRAG 2 cut(s) 29, 323
Sse9I AATT 1 cut(s) 228
SsiI CCGC 4 cut(s) 93, 96, 184, 190
SstI GAGCTC 1 cut(s) 206
StyI CCWWGG 1 cut(s) 351
TaiI ACGT 2 cut(s) 174, 213
TaqI TCGA 1 cut(s) 303
TasI AATT 1 cut(s) 228
TauI GCSGC 2 cut(s) 95, 98
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 1 cut(s) 147
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 280
TspRI CASTG 1 cut(s) 147
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.