Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites and are then released
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G36990 |
| fragaria_vesca | FvH4_6g16180 FvH4_6g16180 FvH4_6g16180 FvH4_6g16180 FvH4_6g16180 FvH4_6g16180 |
| malus_domestica | MD12G1115000.v1.1 |
| prunus_persica | Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 |
| pyrus_communis | pycom04g09010 pycom12g11420 |
| rosa_chinensis | RchiOBHm_Chr3g0470031 |
| rosa_laevigata | RLG00000024302 |
| rosa_multiflora | Rmu_co8112822.1_g000001 Rmu_co8234937.1_g000001 Rmu_sc0017440.1_g000004 Rmu_sc0032107.1_g000001 |
| rosa_roxburghii | Rroxscaffold_6G00411270 |
| rosa_rugosa | Rorug03G0106100 Rorug03G0106100 Rorug03G0106200 |
| rosa_samantha | Rh3AG155500 Rh3BG179600 Rh3CG170200 Rh3DG195500 |
| rosa_wichuraiana | Rw3G014640 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 158 |
| Acc16I | TGCGCA | 1 cut(s) | 1454 |
| AccI | GTMKAC | 2 cut(s) | 838, 1188 |
| AccII | CGCG | 1 cut(s) | 201 |
| AciI | CCGC | 3 cut(s) | 604, 827, 1171 |
| AclWI | GGATC | 2 cut(s) | 716, 1402 |
| AcoI | YGGCCR | 1 cut(s) | 856 |
| AcsI | RAATTY | 3 cut(s) | 376, 628, 1073 |
| AcuI | CTGAAG | 6 cut(s) | 18, 81, 162, 906, 1007, 1628 |
| AfaI | GTAC | 3 cut(s) | 687, 937, 1581 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 60, 805, 1088, 1177, 1204, 1575 |
| AflII | CTTAAG | 1 cut(s) | 617 |
| AflIII | ACRYGT | 1 cut(s) | 1447 |
| AjnI | CCWGG | 2 cut(s) | 1160, 1377 |
| AluBI | AGCT | 8 cut(s) | 125, 503, 510, 527, 890, 1088, 1435, 1612 |
| AluI | AGCT | 8 cut(s) | 125, 503, 510, 527, 890, 1088, 1435, 1612 |
| Alw21I | GWGCWC | 1 cut(s) | 987 |
| Alw26I | GTCTC | 4 cut(s) | 653, 751, 776, 1120 |
| Alw44I | GTGCAC | 1 cut(s) | 983 |
| AlwI | GGATC | 2 cut(s) | 716, 1402 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 128 |
| Ama87I | CYCGRG | 1 cut(s) | 798 |
| AoxI | GGCC | 5 cut(s) | 252, 477, 856, 1018, 1143 |
| ApaLI | GTGCAC | 1 cut(s) | 983 |
| ApeKI | GCWGC | 7 cut(s) | 122, 196, 491, 527, 942, 1432, 1629 |
| ApoI | RAATTY | 3 cut(s) | 376, 628, 1073 |
| AspLEI | GCGC | 3 cut(s) | 201, 609, 1455 |
| AspS9I | GGNCC | 1 cut(s) | 746 |
| AsuHPI | GGTGA | 4 cut(s) | 383, 841, 1135, 1562 |
| AsuII | TTCGAA | 1 cut(s) | 398 |
| AvaI | CYCGRG | 1 cut(s) | 798 |
| AvaII | GGWCC | 1 cut(s) | 746 |
| BaeGI | GKGCMC | 1 cut(s) | 987 |
| Bbv12I | GWGCWC | 1 cut(s) | 987 |
| BbvCI | CCTCAGC | 1 cut(s) | 291 |
| BbvI | GCAGC | 7 cut(s) | 134, 208, 503, 514, 954, 1444, 1641 |
| BccI | CCATC | 3 cut(s) | 52, 253, 1514 |
| BceAI | ACGGC | 3 cut(s) | 843, 930, 1598 |
| BciT130I | CCWGG | 2 cut(s) | 1162, 1379 |
| BcoDI | GTCTC | 4 cut(s) | 653, 751, 776, 1120 |
| BfaI | CTAG | 3 cut(s) | 596, 663, 699 |
| BfmI | CTRYAG | 1 cut(s) | 1034 |
| BfrI | CTTAAG | 1 cut(s) | 617 |
| BisI | GCNGC | 8 cut(s) | 123, 197, 492, 528, 605, 943, 1433, 1630 |
| BlsI | GCNGC | 8 cut(s) | 124, 198, 493, 529, 606, 944, 1434, 1631 |
| Bme1390I | CCNGG | 2 cut(s) | 1162, 1379 |
| Bme18I | GGWCC | 1 cut(s) | 746 |
| BmeT110I | CYCGRG | 1 cut(s) | 798 |
| BmgT120I | GGNCC | 1 cut(s) | 746 |
| BmiI | GGNNCC | 1 cut(s) | 747 |
| BmrFI | CCNGG | 2 cut(s) | 1162, 1379 |
| BmrI | ACTGGG | 1 cut(s) | 1407 |
| BmsI | GCATC | 4 cut(s) | 73, 96, 258, 436 |
| BmuI | ACTGGG | 1 cut(s) | 1407 |
| BpmI | CTGGAG | 1 cut(s) | 1610 |
| Bpu10I | CCTNAGC | 1 cut(s) | 291 |
| Bpu14I | TTCGAA | 1 cut(s) | 398 |
| BpuEI | CTTGAG | 1 cut(s) | 1598 |
| BsaAI | YACGTR | 1 cut(s) | 974 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1406, 1537 |
| BsaJI | CCNNGG | 3 cut(s) | 1198, 1569, 1633 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 60, 805, 1088, 1177, 1204, 1575 |
| Bse1I | ACTGG | 4 cut(s) | 343, 1121, 1402, 1593 |
| Bse3DI | GCAATG | 2 cut(s) | 411, 987 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1406, 1537 |
| BseBI | CCWGG | 2 cut(s) | 1162, 1379 |
| BseDI | CCNNGG | 3 cut(s) | 1198, 1569, 1633 |
| BseGI | GGATG | 4 cut(s) | 64, 273, 380, 814 |
| BseJI | GATNNNNATC | 2 cut(s) | 1406, 1537 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 60, 805, 1088, 1177, 1204, 1575 |
| BseMI | GCAATG | 2 cut(s) | 411, 987 |
| BseMII | CTCAG | 4 cut(s) | 73, 263, 282, 777 |
| BseNI | ACTGG | 4 cut(s) | 343, 1121, 1402, 1593 |
| BseRI | GAGGAG | 1 cut(s) | 66 |
| BseSI | GKGCMC | 1 cut(s) | 987 |
| BseXI | GCAGC | 7 cut(s) | 134, 208, 503, 514, 954, 1444, 1641 |
| BsgI | GTGCAG | 1 cut(s) | 114 |
| Bsh1236I | CGCG | 1 cut(s) | 201 |
| Bsh1285I | CGRYCG | 1 cut(s) | 522 |
| BshFI | GGCC | 5 cut(s) | 254, 479, 858, 1020, 1145 |
| BsiEI | CGRYCG | 1 cut(s) | 522 |
| BsiHKAI | GWGCWC | 1 cut(s) | 987 |
| BsiHKCI | CYCGRG | 1 cut(s) | 798 |
| BsiSI | CCGG | 1 cut(s) | 1178 |
| BslFI | GGGAC | 2 cut(s) | 439, 759 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 60, 805, 1088, 1177, 1204, 1575 |
| BsmAI | GTCTC | 4 cut(s) | 653, 751, 776, 1120 |
| BsmFI | GGGAC | 2 cut(s) | 439, 759 |
| BsmI | GAATGC | 1 cut(s) | 1332 |
| BsnI | GGCC | 5 cut(s) | 254, 479, 858, 1020, 1145 |
| BsoBI | CYCGRG | 1 cut(s) | 798 |
| Bsp119I | TTCGAA | 1 cut(s) | 398 |
| Bsp1286I | GDGCHC | 1 cut(s) | 987 |
| Bsp143I | GATC | 5 cut(s) | 262, 519, 721, 1407, 1532 |
| BspACI | CCGC | 3 cut(s) | 604, 827, 1171 |
| BspANI | GGCC | 5 cut(s) | 254, 479, 858, 1020, 1145 |
| BspCNI | CTCAG | 4 cut(s) | 72, 262, 283, 778 |
| BspFNI | CGCG | 1 cut(s) | 201 |
| BspHI | TCATGA | 2 cut(s) | 112, 655 |
| BspLI | GGNNCC | 1 cut(s) | 747 |
| BspMAI | CTGCAG | 1 cut(s) | 1038 |
| BspPI | GGATC | 2 cut(s) | 716, 1402 |
| BspT104I | TTCGAA | 1 cut(s) | 398 |
| BspTI | CTTAAG | 1 cut(s) | 617 |
| BsrDI | GCAATG | 2 cut(s) | 411, 987 |
| BsrI | ACTGG | 4 cut(s) | 343, 1121, 1402, 1593 |
| BssECI | CCNNGG | 3 cut(s) | 1198, 1569, 1633 |
| BssMI | GATC | 5 cut(s) | 262, 519, 721, 1407, 1532 |
| BssNAI | GTATAC | 1 cut(s) | 1189 |
| BssT1I | CCWWGG | 3 cut(s) | 1198, 1569, 1633 |
| Bst1107I | GTATAC | 1 cut(s) | 1189 |
| Bst2UI | CCWGG | 2 cut(s) | 1162, 1379 |
| Bst4CI | ACNGT | 3 cut(s) | 577, 1351, 1566 |
| Bst6I | CTCTTC | 5 cut(s) | 75, 231, 561, 774, 827 |
| BstAFI | CTTAAG | 1 cut(s) | 617 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 128, 1450 |
| BstBAI | YACGTR | 1 cut(s) | 974 |
| BstBI | TTCGAA | 1 cut(s) | 398 |
| BstC8I | GCNNGC | 3 cut(s) | 120, 611, 1175 |
| BstDEI | CTNAG | 5 cut(s) | 59, 249, 291, 786, 1502 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1202 |
| BstF5I | GGATG | 4 cut(s) | 64, 273, 380, 814 |
| BstFNI | CGCG | 1 cut(s) | 201 |
| BstHHI | GCGC | 3 cut(s) | 201, 609, 1455 |
| BstKTI | GATC | 5 cut(s) | 265, 522, 724, 1410, 1535 |
| BstMAI | GTCTC | 4 cut(s) | 653, 751, 776, 1120 |
| BstMBI | GATC | 5 cut(s) | 262, 519, 721, 1407, 1532 |
| BstMCI | CGRYCG | 1 cut(s) | 522 |
| BstNI | CCWGG | 2 cut(s) | 1162, 1379 |
| BstNSI | RCATGY | 2 cut(s) | 353, 1451 |
| BstSCI | CCNGG | 2 cut(s) | 1160, 1377 |
| BstSFI | CTRYAG | 1 cut(s) | 1034 |
| BstSLI | GKGCMC | 1 cut(s) | 987 |
| BstSNI | TACGTA | 1 cut(s) | 974 |
| BstUI | CGCG | 1 cut(s) | 201 |
| BstV1I | GCAGC | 7 cut(s) | 134, 208, 503, 514, 954, 1444, 1641 |
| BstX2I | RGATCY | 1 cut(s) | 1407 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 391, 1640 |
| BstYI | RGATCY | 1 cut(s) | 1407 |
| BstZ17I | GTATAC | 1 cut(s) | 1189 |
| BsuRI | GGCC | 5 cut(s) | 254, 479, 858, 1020, 1145 |
| BtgZI | GCGATG | 1 cut(s) | 216 |
| BtsCI | GGATG | 4 cut(s) | 64, 273, 380, 814 |
| BtsI | GCAGTG | 1 cut(s) | 285 |
| BtsIMutI | CAGTG | 2 cut(s) | 285, 1395 |
| Cac8I | GCNNGC | 3 cut(s) | 120, 611, 1175 |
| CaiI | CAGNNNCTG | 1 cut(s) | 128 |
| CciI | TCATGA | 2 cut(s) | 112, 655 |
| CfoI | GCGC | 3 cut(s) | 201, 609, 1455 |
| Cfr13I | GGNCC | 1 cut(s) | 746 |
| Csp6I | GTAC | 3 cut(s) | 686, 936, 1580 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1334, 1369 |
| CviAII | CATG | 5 cut(s) | 113, 350, 441, 656, 1448 |
| CviQI | GTAC | 3 cut(s) | 686, 936, 1580 |
| DdeI | CTNAG | 5 cut(s) | 59, 249, 291, 786, 1502 |
| DpnI | GATC | 5 cut(s) | 264, 521, 723, 1409, 1534 |
| DpnII | GATC | 5 cut(s) | 262, 519, 721, 1407, 1532 |
| EaeI | YGGCCR | 1 cut(s) | 856 |
| Eam1104I | CTCTTC | 5 cut(s) | 75, 231, 561, 774, 827 |
| EarI | CTCTTC | 5 cut(s) | 75, 231, 561, 774, 827 |
| Eco105I | TACGTA | 1 cut(s) | 974 |
| Eco130I | CCWWGG | 3 cut(s) | 1198, 1569, 1633 |
| Eco47I | GGWCC | 1 cut(s) | 746 |
| Eco57I | CTGAAG | 6 cut(s) | 18, 81, 162, 906, 1007, 1628 |
| Eco88I | CYCGRG | 1 cut(s) | 798 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1202 |
| EcoO109I | RGGNCCY | 1 cut(s) | 746 |
| EcoRII | CCWGG | 2 cut(s) | 1160, 1377 |
| EcoT14I | CCWWGG | 3 cut(s) | 1198, 1569, 1633 |
| EcoT22I | ATGCAT | 2 cut(s) | 451, 1114 |
| ErhI | CCWWGG | 3 cut(s) | 1198, 1569, 1633 |
| FaeI | CATG | 5 cut(s) | 116, 353, 444, 659, 1451 |
| FaqI | GGGAC | 2 cut(s) | 439, 759 |
| FatI | CATG | 5 cut(s) | 112, 349, 440, 655, 1447 |
| FauNDI | CATATG | 1 cut(s) | 426 |
| FblI | GTMKAC | 2 cut(s) | 838, 1188 |
| Fnu4HI | GCNGC | 8 cut(s) | 123, 197, 492, 528, 605, 943, 1433, 1630 |
| FokI | GGATG | 4 cut(s) | 51, 280, 367, 821 |
| Fsp4HI | GCNGC | 8 cut(s) | 123, 197, 492, 528, 605, 943, 1433, 1630 |
| FspBI | CTAG | 3 cut(s) | 596, 663, 699 |
| FspI | TGCGCA | 1 cut(s) | 1454 |
| GlaI | GCGC | 3 cut(s) | 200, 608, 1454 |
| GluI | GCNGC | 8 cut(s) | 123, 197, 492, 528, 605, 943, 1433, 1630 |
| GsuI | CTGGAG | 1 cut(s) | 1610 |
| HaeIII | GGCC | 5 cut(s) | 254, 479, 858, 1020, 1145 |
| HapII | CCGG | 1 cut(s) | 1178 |
| HhaI | GCGC | 3 cut(s) | 201, 609, 1455 |
| Hin1II | CATG | 5 cut(s) | 116, 353, 444, 659, 1451 |
| Hin6I | GCGC | 3 cut(s) | 199, 607, 1453 |
| HinP1I | GCGC | 3 cut(s) | 199, 607, 1453 |
| HincII | GTYRAC | 1 cut(s) | 585 |
| HindII | GTYRAC | 1 cut(s) | 585 |
| HinfI | GANTC | 2 cut(s) | 539, 1619 |
| HpaII | CCGG | 1 cut(s) | 1178 |
| HphI | GGTGA | 4 cut(s) | 383, 841, 1135, 1562 |
| Hpy166II | GTNNAC | 5 cut(s) | 585, 839, 863, 985, 1189 |
| Hpy188I | TCNGA | 6 cut(s) | 267, 524, 682, 909, 1543, 1608 |
| Hpy188III | TCNNGA | 8 cut(s) | 103, 113, 596, 656, 806, 1025, 1235, 1472 |
| Hpy8I | GTNNAC | 5 cut(s) | 585, 839, 863, 985, 1189 |
| HpyAV | CCTTC | 6 cut(s) | 6, 90, 96, 1031, 1135, 1232 |
| HpyCH4III | ACNGT | 3 cut(s) | 577, 1351, 1566 |
| HpyCH4IV | ACGT | 1 cut(s) | 973 |
| HpyF3I | CTNAG | 5 cut(s) | 59, 249, 291, 786, 1502 |
| HpySE526I | ACGT | 1 cut(s) | 973 |
| Hsp92II | CATG | 5 cut(s) | 116, 353, 444, 659, 1451 |
| HspAI | GCGC | 3 cut(s) | 199, 607, 1453 |
| Kzo9I | GATC | 5 cut(s) | 262, 519, 721, 1407, 1532 |
| LmnI | GCTCC | 4 cut(s) | 130, 515, 524, 1657 |
| Lsp1109I | GCAGC | 7 cut(s) | 134, 208, 503, 514, 954, 1444, 1641 |
| LweI | GCATC | 4 cut(s) | 73, 96, 258, 436 |
| MaeI | CTAG | 3 cut(s) | 596, 663, 699 |
| MaeII | ACGT | 1 cut(s) | 973 |
| MaeIII | GTNAC | 2 cut(s) | 701, 1384 |
| MalI | GATC | 5 cut(s) | 264, 521, 723, 1409, 1534 |
| MboI | GATC | 5 cut(s) | 262, 519, 721, 1407, 1532 |
| MflI | RGATCY | 1 cut(s) | 1407 |
| MhlI | GDGCHC | 1 cut(s) | 987 |
| MluCI | AATT | 8 cut(s) | 376, 628, 651, 1073, 1134, 1265, 1298, 1307 |
| MlyI | GAGTC | 1 cut(s) | 548 |
| MmeI | TCCRAC | 2 cut(s) | 537, 1261 |
| Mph1103I | ATGCAT | 2 cut(s) | 451, 1114 |
| MseI | TTAA | 8 cut(s) | 231, 435, 534, 618, 636, 710, 774, 1666 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1117 |
| MspA1I | CMGCKG | 1 cut(s) | 1435 |
| MspCI | CTTAAG | 1 cut(s) | 617 |
| MspI | CCGG | 1 cut(s) | 1178 |
| MspR9I | CCNGG | 2 cut(s) | 1162, 1379 |
| Mva1269I | GAATGC | 1 cut(s) | 1332 |
| MvaI | CCWGG | 2 cut(s) | 1162, 1379 |
| MvnI | CGCG | 1 cut(s) | 201 |
| NdeI | CATATG | 1 cut(s) | 426 |
| NdeII | GATC | 5 cut(s) | 262, 519, 721, 1407, 1532 |
| NlaIII | CATG | 5 cut(s) | 116, 353, 444, 659, 1451 |
| NlaIV | GGNNCC | 1 cut(s) | 747 |
| NmeAIII | GCCGAG | 1 cut(s) | 1622 |
| NmuCI | GTSAC | 1 cut(s) | 701 |
| NsbI | TGCGCA | 1 cut(s) | 1454 |
| NsiI | ATGCAT | 2 cut(s) | 451, 1114 |
| NspI | RCATGY | 2 cut(s) | 353, 1451 |
| NspV | TTCGAA | 1 cut(s) | 398 |
| PagI | TCATGA | 2 cut(s) | 112, 655 |
| PciI | ACATGT | 1 cut(s) | 1447 |
| PctI | GAATGC | 1 cut(s) | 1332 |
| PfeI | GAWTC | 1 cut(s) | 1619 |
| PfoI | TCCNGGA | 2 cut(s) | 1160, 1377 |
| PkrI | GCNGC | 8 cut(s) | 124, 198, 493, 529, 606, 944, 1434, 1631 |
| Ple19I | CGATCG | 1 cut(s) | 522 |
| PleI | GAGTC | 1 cut(s) | 547 |
| PpsI | GAGTC | 1 cut(s) | 547 |
| Ppu21I | YACGTR | 1 cut(s) | 974 |
| PpuMI | RGGWCCY | 1 cut(s) | 746 |
| PscI | ACATGT | 1 cut(s) | 1447 |
| PsiI | TTATAA | 1 cut(s) | 158 |
| Psp5II | RGGWCCY | 1 cut(s) | 746 |
| Psp6I | CCWGG | 2 cut(s) | 1160, 1377 |
| PspGI | CCWGG | 2 cut(s) | 1160, 1377 |
| PspN4I | GGNNCC | 1 cut(s) | 747 |
| PspPI | GGNCC | 1 cut(s) | 746 |
| PspPPI | RGGWCCY | 1 cut(s) | 746 |
| PstI | CTGCAG | 1 cut(s) | 1038 |
| PstNI | CAGNNNCTG | 1 cut(s) | 128 |
| PsuI | RGATCY | 1 cut(s) | 1407 |
| PvuI | CGATCG | 1 cut(s) | 522 |
| PvuII | CAGCTG | 1 cut(s) | 1435 |
| RsaI | GTAC | 3 cut(s) | 687, 937, 1581 |
| RsaNI | GTAC | 3 cut(s) | 686, 936, 1580 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1117 |
| SaqAI | TTAA | 8 cut(s) | 231, 435, 534, 618, 636, 710, 774, 1666 |
| SatI | GCNGC | 8 cut(s) | 123, 197, 492, 528, 605, 943, 1433, 1630 |
| Sau3AI | GATC | 5 cut(s) | 262, 519, 721, 1407, 1532 |
| Sau96I | GGNCC | 1 cut(s) | 746 |
| SchI | GAGTC | 1 cut(s) | 548 |
| ScrFI | CCNGG | 2 cut(s) | 1162, 1379 |
| SduI | GDGCHC | 1 cut(s) | 987 |
| SfaNI | GCATC | 4 cut(s) | 73, 96, 258, 436 |
| SfcI | CTRYAG | 1 cut(s) | 1034 |
| SfuI | TTCGAA | 1 cut(s) | 398 |
| SinI | GGWCC | 1 cut(s) | 746 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1117 |
| SmlI | CTYRAG | 2 cut(s) | 617, 1613 |
| SmoI | CTYRAG | 2 cut(s) | 617, 1613 |
| SnaBI | TACGTA | 1 cut(s) | 974 |
| Sse9I | AATT | 8 cut(s) | 376, 628, 651, 1073, 1134, 1265, 1298, 1307 |
| SsiI | CCGC | 3 cut(s) | 604, 827, 1171 |
| SspMI | CTAG | 3 cut(s) | 596, 663, 699 |
| StyD4I | CCNGG | 2 cut(s) | 1160, 1377 |
| StyI | CCWWGG | 3 cut(s) | 1198, 1569, 1633 |
| TaaI | ACNGT | 3 cut(s) | 577, 1351, 1566 |
| TaiI | ACGT | 1 cut(s) | 976 |
| TaqI | TCGA | 4 cut(s) | 163, 398, 518, 816 |
| TasI | AATT | 8 cut(s) | 376, 628, 651, 1073, 1134, 1265, 1298, 1307 |
| TauI | GCSGC | 1 cut(s) | 607 |
| TfiI | GAWTC | 1 cut(s) | 1619 |
| Tru1I | TTAA | 8 cut(s) | 231, 435, 534, 618, 636, 710, 774, 1666 |
| Tru9I | TTAA | 8 cut(s) | 231, 435, 534, 618, 636, 710, 774, 1666 |
| TscAI | CASTG | 2 cut(s) | 292, 1402 |
| TseFI | GTSAC | 1 cut(s) | 701 |
| TseI | GCWGC | 7 cut(s) | 122, 196, 491, 527, 942, 1432, 1629 |
| Tsp45I | GTSAC | 1 cut(s) | 701 |
| TspDTI | ATGAA | 5 cut(s) | 18, 101, 369, 644, 1073 |
| TspGWI | ACGGA | 2 cut(s) | 703, 720 |
| TspRI | CASTG | 2 cut(s) | 292, 1402 |
| Vha464I | CTTAAG | 1 cut(s) | 617 |
| VneI | GTGCAC | 1 cut(s) | 983 |
| VpaK11BI | GGWCC | 1 cut(s) | 746 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1202 |
| XapI | RAATTY | 3 cut(s) | 376, 628, 1073 |
| XbaI | TCTAGA | 1 cut(s) | 595 |
| XceI | RCATGY | 2 cut(s) | 353, 1451 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 973 |
| XmiI | GTMKAC | 2 cut(s) | 838, 1188 |
| XspI | CTAG | 3 cut(s) | 596, 663, 699 |
| Zsp2I | ATGCAT | 2 cut(s) | 451, 1114 |