Rmu_sc0032107.1_g000001

Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites and are then released

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032107.1
OG0004828
Physical Location & Seq
Forward (+)
1 .. 1606
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032107.1_g000001.1.cds

Sequence Viewer

Length: 611 bp
gagggaagtatgggtctaatgaaaagtgttgagaaattcaaaccccaagctggttgccgatttgctacttatgcatactggtgggtgagacaaacaattaggaaggccatatttcaacattccaggactatccgcttgccggagaatgtatacaccctccttggcaaggtattggaggcaaagaaatcatatattcaggaaggaaatcacaacccaagcagagaagaattagcaagacgagttggaatgtcggttgaaaaattgggaaaattgctatattctacaagaatgcctgtttcaatgcaacaaaccgtgtgggcagaccaagataccactttccaggaggtaactgctgacactgggattgagatcccagatgtaagtgtggcaaagcagctgatgaggcaacatgtgcgcaacctcctacacactcttgaaaaaagagagaggcagataatcaggctaagatatggtattgaagatggtaagcagagatcactatcagaggttggtgagatttttggactgtccaaggaacgggtacggcaactggagagccgagcatttctgaagctcaaggaatcacttggcagccaagggcttggggctta
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

203

Amino Acids

23.35

Weight (kDa)

9.87

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 416
AccI GTMKAC 1 cut(s) 150
AciI CCGC 1 cut(s) 133
AclWI GGATC 1 cut(s) 364
AcsI RAATTY 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 590
AfaI GTAC 1 cut(s) 543
AfiI CCNNNNNNNGG 4 cut(s) 50, 139, 166, 537
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 6 cut(s) 40, 116, 257, 300, 437, 479
AjnI CCWGG 2 cut(s) 122, 339
AluBI AGCT 3 cut(s) 50, 397, 574
AluI AGCT 3 cut(s) 50, 397, 574
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 1 cut(s) 364
AoxI GGCC 1 cut(s) 105
ApeKI GCWGC 2 cut(s) 394, 591
ApoI RAATTY 1 cut(s) 35
AspLEI GCGC 1 cut(s) 417
AsuHPI GGTGA 2 cut(s) 97, 524
BbvI GCAGC 2 cut(s) 406, 603
BccI CCATC 1 cut(s) 476
BceAI ACGGC 1 cut(s) 560
BciT130I CCWGG 2 cut(s) 124, 341
BcoDI GTCTC 1 cut(s) 82
BisI GCNGC 2 cut(s) 395, 592
BlsI GCNGC 2 cut(s) 396, 593
Bme1390I CCNGG 2 cut(s) 124, 341
BmrFI CCNGG 2 cut(s) 124, 341
BmrI ACTGGG 1 cut(s) 369
BmuI ACTGGG 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 560
BsaBI GATNNNNATC 2 cut(s) 368, 499
BsaJI CCNNGG 3 cut(s) 160, 531, 595
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 139, 166, 537
Bse1I ACTGG 3 cut(s) 83, 364, 555
Bse8I GATNNNNATC 2 cut(s) 368, 499
BseBI CCWGG 2 cut(s) 124, 341
BseDI CCNNGG 3 cut(s) 160, 531, 595
BseJI GATNNNNATC 2 cut(s) 368, 499
BseLI CCNNNNNNNGG 4 cut(s) 50, 139, 166, 537
BseNI ACTGG 3 cut(s) 83, 364, 555
BseXI GCAGC 2 cut(s) 406, 603
BshFI GGCC 1 cut(s) 107
BsiSI CCGG 1 cut(s) 140
BslI CCNNNNNNNGG 4 cut(s) 50, 139, 166, 537
BsmAI GTCTC 1 cut(s) 82
BsmI GAATGC 1 cut(s) 294
BsnI GGCC 1 cut(s) 107
Bsp143I GATC 2 cut(s) 369, 494
BspACI CCGC 1 cut(s) 133
BspANI GGCC 1 cut(s) 107
BspPI GGATC 1 cut(s) 364
BsrI ACTGG 3 cut(s) 83, 364, 555
BssECI CCNNGG 3 cut(s) 160, 531, 595
BssMI GATC 2 cut(s) 369, 494
BssNAI GTATAC 1 cut(s) 151
BssT1I CCWWGG 3 cut(s) 160, 531, 595
Bst1107I GTATAC 1 cut(s) 151
Bst2UI CCWGG 2 cut(s) 124, 341
Bst4CI ACNGT 2 cut(s) 313, 528
BstAPI GCANNNNNTGC 1 cut(s) 412
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 464
BstENI CCTNNNNNAGG 1 cut(s) 164
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 2 cut(s) 372, 497
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 2 cut(s) 369, 494
BstMWI GCNNNNNNNGC 3 cut(s) 71, 403, 412
BstNI CCWGG 2 cut(s) 124, 341
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 2 cut(s) 122, 339
BstV1I GCAGC 2 cut(s) 406, 603
BstX2I RGATCY 1 cut(s) 369
BstXI CCANNNNNNTGG 1 cut(s) 602
BstYI RGATCY 1 cut(s) 369
BstZ17I GTATAC 1 cut(s) 151
BsuRI GGCC 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 137
CfoI GCGC 1 cut(s) 417
Csp6I GTAC 1 cut(s) 542
CspCI CAANNNNNGTGG 2 cut(s) 296, 331
CviAII CATG 1 cut(s) 410
CviJI RGCY 9 cut(s) 50, 107, 397, 463, 558, 574, 594, 601, 608
CviKI_1 RGCY 9 cut(s) 50, 107, 397, 463, 558, 574, 594, 601, 608
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 1 cut(s) 464
DpnI GATC 2 cut(s) 371, 496
DpnII GATC 2 cut(s) 369, 494
Eco130I CCWWGG 3 cut(s) 160, 531, 595
Eco57I CTGAAG 1 cut(s) 590
EcoNI CCTNNNNNAGG 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 122, 339
EcoT14I CCWWGG 3 cut(s) 160, 531, 595
EcoT22I ATGCAT 1 cut(s) 76
ErhI CCWWGG 3 cut(s) 160, 531, 595
FaeI CATG 1 cut(s) 413
FatI CATG 1 cut(s) 409
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 395, 592
Fsp4HI GCNGC 2 cut(s) 395, 592
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 395, 592
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 1 cut(s) 107
HapII CCGG 1 cut(s) 140
HhaI GCGC 1 cut(s) 417
Hin1II CATG 1 cut(s) 413
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HinfI GANTC 1 cut(s) 581
HpaII CCGG 1 cut(s) 140
HphI GGTGA 2 cut(s) 97, 524
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 2 cut(s) 505, 570
Hpy188III TCNNGA 2 cut(s) 197, 434
Hpy8I GTNNAC 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 97, 194
HpyCH4III ACNGT 2 cut(s) 313, 528
HpyCH4V TGCA 2 cut(s) 74, 304
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 403, 412
HpyF3I CTNAG 1 cut(s) 464
Hsp92II CATG 1 cut(s) 413
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 2 cut(s) 369, 494
Lsp1109I GCAGC 2 cut(s) 406, 603
MaeIII GTNAC 1 cut(s) 346
MalI GATC 2 cut(s) 371, 496
MboI GATC 2 cut(s) 369, 494
MboII GAAGA 2 cut(s) 236, 491
MflI RGATCY 1 cut(s) 369
MluCI AATT 5 cut(s) 35, 96, 227, 260, 269
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 7 cut(s) 167, 169, 337, 396, 431, 441, 499
Mph1103I ATGCAT 1 cut(s) 76
MslI CAYNNNNRTG 1 cut(s) 79
MspA1I CMGCKG 1 cut(s) 397
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 2 cut(s) 124, 341
Mva1269I GAATGC 1 cut(s) 294
MvaI CCWGG 2 cut(s) 124, 341
MwoI GCNNNNNNNGC 3 cut(s) 71, 403, 412
NdeII GATC 2 cut(s) 369, 494
NlaIII CATG 1 cut(s) 413
NmeAIII GCCGAG 1 cut(s) 584
NsbI TGCGCA 1 cut(s) 416
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 1 cut(s) 413
PciI ACATGT 1 cut(s) 409
PctI GAATGC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 581
PfoI TCCNGGA 2 cut(s) 122, 339
PkrI GCNGC 2 cut(s) 396, 593
PscI ACATGT 1 cut(s) 409
Psp6I CCWGG 2 cut(s) 122, 339
PspGI CCWGG 2 cut(s) 122, 339
PsuI RGATCY 1 cut(s) 369
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
RseI CAYNNNNRTG 1 cut(s) 79
SatI GCNGC 2 cut(s) 395, 592
Sau3AI GATC 2 cut(s) 369, 494
ScrFI CCNGG 2 cut(s) 124, 341
SetI ASST 7 cut(s) 52, 171, 348, 399, 423, 510, 576
SmiMI CAYNNNNRTG 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 575
SmoI CTYRAG 1 cut(s) 575
Sse9I AATT 5 cut(s) 35, 96, 227, 260, 269
SsiI CCGC 1 cut(s) 133
StyD4I CCNGG 2 cut(s) 122, 339
StyI CCWWGG 3 cut(s) 160, 531, 595
TaaI ACNGT 2 cut(s) 313, 528
TasI AATT 5 cut(s) 35, 96, 227, 260, 269
TfiI GAWTC 1 cut(s) 581
TscAI CASTG 1 cut(s) 364
TseI GCWGC 2 cut(s) 394, 591
TspDTI ATGAA 1 cut(s) 35
TspRI CASTG 1 cut(s) 364
XagI CCTNNNNNAGG 1 cut(s) 164
XapI RAATTY 1 cut(s) 35
XceI RCATGY 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 150
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.