Rorug03G0106200

Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites and are then released

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004828
Physical Location & Seq
Forward (+)
8514010 .. 8517743
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0106200.1

Sequence Viewer

Length: 2052 bp
ATGGCAACAACAACAACAACAAAACTGAAACGCTCTTCTTGCCTTCAATCCATCATCAAGAGCCAAAAGGCCGTTTTGAACACCCACACCAACTTCTTCCATTTCCTCTCAGATGAACTCATCTTCATAATCCTGGACTTCCTCACCCCAAACCCAATTGACAGAAAGTCCTTCTCTCTAGTCTGCAAGTCCTTCTACTCCCTAGAATCCAAACACCGCCGCACCCTGACCCCTCTCCGCTCCGACCACCTCCCCACAATCCTGACCCGGTACCCATCTGTCACCCGCGTCGATCTCACGCTCTGCCCTCGGATCACAGACGCTTCCCTCATCACAATCTCAAATGCGTGCAAGTCCAACCTCCAAAGCATCGATCTTTCCCGGTCCAAGTACTTCTCCGGAGCCGGGCTACAGAGCTTGGCCCTGAATTGCAAGAATATGGTGGAGATTGACTTATCCAATGCGACGGAGCTCCGAGACTCGGCCGCTGCGGCTCTGGCCGAGGCCAAGAACTTGGAGAGGCTCTGGTTGGGAAGGTGCAAGATGATTACGGACATGGGTGTGGGGTGCATAGCGGTTGGTTGTAGGAAGTTGAAATTGATTAGCTTAAAATGGTGCCTCGGTGTTGGTGACTTGGGAATTGAGTTGCTTGCGCTCAAGTGTAAAGATCTTCAAAGTTTGGATCTGTCTTACCTGCCGATCACAGAAAAATGTTTACCGTCGATCTTCAAGTTACCGCATCTTGAGGATTTGGTTCTAGAAGGATGCTTTGGCATCGATGATGACAGCCTTGCAGCCCTCAAACATGGGTGCAAGTCGCTAAAGAAGCTTGACATATCAAGTTGTCAGAACATTAGTCATAATGGTTTGGCTTCCCTAACTAGTGGTGGAGTTCTAGAGCAACTCGTATTGTCCCATGGCTCTCCTGTGACTCTTGCTCTTGCTGATAGTTTGAAAAAGCTACCGATGTTGCAGTCAATCAAATTAGATGGTTGCTCAATTACTTATGCTGGACTGCAAGCGATTGGAAACTGGTGTGTGTCATTAAGGGAGCTGAGCTTAAGTAAATGTCCTGGGGTGACAGATGAGGGGCTCTCCTCCCTTTTGACGAAACACAAAGATCTGCGGAAGTTAGACATCACCTGTTGCCGAATGATAACTTATACTTCCATAGATCGTATTACATTATCATGCACCGCACTCACTTCTCTTAGGATGGAGTCATGCACCCTGGTTCCAAGAGAAGCATTCGTCCTCATTGGGCAGAGATGCCGTTTTCTTGAGGAGATTGACATAACAGATAATGAAGTTGATGATGAAGGTTTGAAGTCGATCTCCAGATGTTCAGAACTCTCCAGCTTAAAACTAGGAATTTGCCTAAACATAACTGACGAGGGAGTTGCTCAGGTTGGCATGTGCTGCTCGAAACTTGTAGAGCTCGATTTGTACAGGTGCACGGGAATTTCAGATTCAGGCATTTCAGCCATTGCTCGTGGTTGTCCTGGGCTCGAGATGATTAATATAGCTTACTGTAAAGACATTACTGATAGTTCACTAATCTCATTGTCGAAATGCTCAAGTTTGAACACAGTAGAAAGTCGAGGATGTCCTCTTATCACATCTTTGGGTCTAGCTGCAATTGCTGTGGGATGCAAGCAATTGGTCAAGCTGGACATAAAAAAGTGCATCAATATTGATGATGCTGGGATGATCCCGCTTGCTCACTTCTCTCAGAACCTTAGACAGATTAATTTGTCATATACTTCTGTTACGGATGTCGGGCTGTTGTCCCTAGCCAGCATCAGCTGCCTGCAGAGCTTAACCATCTTGCATTTGAAGGGCTTGACCGCTAGTGGGCTTGCAGCTGCCTTATTGGTATGCGGAGGGCTAACAAAAGTAAAGCTCCAGGCAACCTTCAAGTCATTGTTACCCCAAGCTCTTTTCGAACATTTAGAAGCACGCGGTTGTCTGTTTCTGTGGAGAGATAAATTTTTCCGGGCTGAACTCGACCCTCAGTGTTGGAAATTACAGTTGGAAGATAACATACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

683

Amino Acids

74.46

Weight (kDa)

8.13

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 101 - 203 2.8e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 220 - 299 1.3e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 302 - 413 6.7e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 360 - 496 3.1e-13 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 535 - 630 6.8e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 702
Acc65I GGTACC 1 cut(s) 270
AccB1I GGYRCC 2 cut(s) 270, 615
AccBSI CCGCTC 1 cut(s) 240
AccII CGCG 2 cut(s) 288, 1962
AccIII TCCGGA 1 cut(s) 398
AclWI GGATC 3 cut(s) 320, 690, 1705
AcoI YGGCCR 2 cut(s) 483, 498
AcsI RAATTY 3 cut(s) 1371, 1461, 1988
AfaI GTAC 3 cut(s) 272, 392, 1448
AfiI CCNNNNNNNGG 3 cut(s) 405, 481, 1854
AflII CTTAAG 1 cut(s) 1060
AhdI GACNNNNNGTC 1 cut(s) 166
AhlI ACTAGT 1 cut(s) 881
AjnI CCWGG 5 cut(s) 132, 1072, 1230, 1501, 1905
AjuI GAANNNNNNNTTGG 4 cut(s) 753, 785, 2015, 2047
Alw21I GWGCWC 3 cut(s) 474, 1440, 1457
Alw26I GTCTC 1 cut(s) 471
Alw44I GTGCAC 1 cut(s) 1453
AlwI GGATC 3 cut(s) 320, 690, 1705
Ama87I CYCGRG 1 cut(s) 1508
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 5 cut(s) 69, 420, 483, 498, 504
ApaLI GTGCAC 1 cut(s) 1453
ApeKI GCWGC 7 cut(s) 488, 794, 1419, 1634, 1806, 1862, 1865
ApoI RAATTY 3 cut(s) 1371, 1461, 1988
AseI ATTAAT 2 cut(s) 1518, 1749
Asp718I GGTACC 1 cut(s) 270
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 2 cut(s) 384, 421
AsuC2I CCSGG 4 cut(s) 268, 382, 406, 1997
AsuHPI GGTGA 5 cut(s) 136, 274, 641, 1090, 1132
AsuII TTCGAA 1 cut(s) 1944
AvaI CYCGRG 1 cut(s) 1508
AvaII GGWCC 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 1457
BanI GGYRCC 2 cut(s) 270, 615
BanII GRGCYC 4 cut(s) 474, 1095, 1440, 1509
BarI GAAGNNNNNNTAC 1 cut(s) 2028
BauI CACGAG 1 cut(s) 1491
Bbv12I GWGCWC 3 cut(s) 474, 1440, 1457
BbvI GCAGC 7 cut(s) 475, 806, 1406, 1621, 1793, 1852, 1874
BccI CCATC 5 cut(s) 59, 283, 983, 1210, 1832
BceAI ACGGC 2 cut(s) 56, 1257
BcgI CGANNNNNNTGC 2 cut(s) 1382, 1416
BciT130I CCWGG 5 cut(s) 134, 1074, 1232, 1503, 1907
BcnI CCSGG 4 cut(s) 268, 382, 406, 1997
BcoDI GTCTC 1 cut(s) 471
BcuI ACTAGT 1 cut(s) 881
BfaI CTAG 9 cut(s) 179, 203, 758, 882, 896, 1367, 1631, 1793, 1851
BfmI CTRYAG 2 cut(s) 410, 1811
BfrI CTTAAG 1 cut(s) 1060
BfuAI ACCTGC 1 cut(s) 702
BglI GCCNNNNNGGC 1 cut(s) 491
BglII AGATCT 2 cut(s) 667, 1120
BlpI GCTNAGC 1 cut(s) 1055
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 9 cut(s) 134, 268, 382, 406, 1074, 1232, 1503, 1907, 1997
Bme18I GGWCC 1 cut(s) 384
BmeRI GACNNNNNGTC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 1508
BmgT120I GGNCC 2 cut(s) 384, 421
BmiI GGNNCC 4 cut(s) 272, 403, 617, 1236
BmrFI CCNGG 9 cut(s) 134, 268, 382, 406, 1074, 1232, 1503, 1907, 1997
BmsI GCATC 9 cut(s) 378, 748, 755, 783, 1259, 1640, 1690, 1695, 1809
BplI GAGNNNNNCTC 2 cut(s) 1079, 1111
BpmI CTGGAG 3 cut(s) 1321, 1339, 1889
Bpu10I CCTNAGC 1 cut(s) 1404
Bpu1102I GCTNAGC 1 cut(s) 1055
Bpu14I TTCGAA 1 cut(s) 1944
BpuEI CTTGAG 4 cut(s) 641, 764, 1301, 1561
BpuMI CCSGG 4 cut(s) 268, 382, 406, 1997
Bsa29I ATCGAT 2 cut(s) 372, 777
BsaJI CCNNGG 7 cut(s) 308, 501, 619, 916, 1073, 1230, 1502
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 3 cut(s) 405, 481, 1854
Bse1I ACTGG 1 cut(s) 1037
Bse3DI GCAATG 1 cut(s) 1485
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 5 cut(s) 134, 1074, 1232, 1503, 1907
BseCI ATCGAT 2 cut(s) 372, 777
BseDI CCNNGG 7 cut(s) 308, 501, 619, 916, 1073, 1230, 1502
BseGI GGATG 6 cut(s) 770, 1221, 1610, 1655, 1713, 1780
BseLI CCNNNNNNNGG 3 cut(s) 405, 481, 1854
BseMI GCAATG 1 cut(s) 1485
BseMII CTCAG 5 cut(s) 123, 1046, 1418, 1745, 2027
BseNI ACTGG 1 cut(s) 1037
BseRI GAGGAG 2 cut(s) 1087, 1298
BseSI GKGCMC 1 cut(s) 1457
BseX3I CGGCCG 1 cut(s) 483
BseXI GCAGC 7 cut(s) 475, 806, 1406, 1621, 1793, 1852, 1874
BseYI CCCAGC 1 cut(s) 1703
Bsh1236I CGCG 2 cut(s) 288, 1962
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 5 cut(s) 71, 422, 485, 500, 506
BshNI GGYRCC 2 cut(s) 270, 615
BshVI ATCGAT 2 cut(s) 372, 777
BsiEI CGRYCG 1 cut(s) 486
BsiHKAI GWGCWC 3 cut(s) 474, 1440, 1457
BsiHKCI CYCGRG 1 cut(s) 1508
BsiSI CCGG 5 cut(s) 268, 382, 399, 405, 1996
BslFI GGGAC 2 cut(s) 898, 1774
BslI CCNNNNNNNGG 3 cut(s) 405, 481, 1854
BsmAI GTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 898, 1774
BsmI GAATGC 1 cut(s) 1247
BsnI GGCC 5 cut(s) 71, 422, 485, 500, 506
BsoBI CYCGRG 1 cut(s) 1508
Bsp119I TTCGAA 1 cut(s) 1944
Bsp1286I GDGCHC 5 cut(s) 474, 1095, 1440, 1457, 1509
Bsp13I TCCGGA 1 cut(s) 398
Bsp1407I TGTACA 1 cut(s) 1446
Bsp1720I GCTNAGC 1 cut(s) 1055
Bsp19I CCATGG 1 cut(s) 916
BspANI GGCC 5 cut(s) 71, 422, 485, 500, 506
BspCNI CTCAG 5 cut(s) 122, 1047, 1417, 1744, 2026
BspDI ATCGAT 2 cut(s) 372, 777
BspEI TCCGGA 1 cut(s) 398
BspFNI CGCG 2 cut(s) 288, 1962
BspLI GGNNCC 4 cut(s) 272, 403, 617, 1236
BspMAI CTGCAG 1 cut(s) 1815
BspMI ACCTGC 1 cut(s) 702
BspPI GGATC 3 cut(s) 320, 690, 1705
BspQI GCTCTTC 1 cut(s) 40
BspT104I TTCGAA 1 cut(s) 1944
BspT107I GGYRCC 2 cut(s) 270, 615
BspTI CTTAAG 1 cut(s) 1060
BsrBI CCGCTC 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 1485
BsrGI TGTACA 1 cut(s) 1446
BsrI ACTGG 1 cut(s) 1037
BssECI CCNNGG 7 cut(s) 308, 501, 619, 916, 1073, 1230, 1502
BssSI CACGAG 1 cut(s) 1491
BssT1I CCWWGG 1 cut(s) 916
Bst2BI CACGAG 1 cut(s) 1491
Bst2UI CCWGG 5 cut(s) 134, 1074, 1232, 1503, 1907
Bst4CI ACNGT 5 cut(s) 720, 1532, 1591, 2031, 2049
Bst6I CTCTTC 1 cut(s) 40
BstAFI CTTAAG 1 cut(s) 1060
BstAPI GCANNNNNTGC 2 cut(s) 1419, 1806
BstAUI TGTACA 1 cut(s) 1446
BstBI TTCGAA 1 cut(s) 1944
BstC8I GCNNGC 9 cut(s) 349, 651, 1020, 1655, 1719, 1798, 1811, 1860, 1960
BstDEI CTNAG 7 cut(s) 109, 1055, 1211, 1404, 1731, 1739, 2013
BstDSI CCRYGG 1 cut(s) 916
BstF5I GGATG 6 cut(s) 770, 1221, 1610, 1655, 1713, 1780
BstFNI CGCG 2 cut(s) 288, 1962
BstHHI GCGC 1 cut(s) 655
BstMAI GTCTC 1 cut(s) 471
BstMCI CGRYCG 1 cut(s) 486
BstMWI GCNNNNNNNGC 8 cut(s) 39, 491, 497, 826, 1419, 1640, 1806, 1815
BstNI CCWGG 5 cut(s) 134, 1074, 1232, 1503, 1907
BstNSI RCATGY 1 cut(s) 1417
BstSCI CCNGG 9 cut(s) 132, 266, 380, 404, 1072, 1230, 1501, 1905, 1995
BstSFI CTRYAG 2 cut(s) 410, 1811
BstSLI GKGCMC 1 cut(s) 1457
BstUI CGCG 2 cut(s) 288, 1962
BstV1I GCAGC 7 cut(s) 475, 806, 1406, 1621, 1793, 1852, 1874
BstX2I RGATCY 3 cut(s) 667, 682, 1120
BstXI CCANNNNNNTGG 1 cut(s) 514
BstYI RGATCY 3 cut(s) 667, 682, 1120
BstZI CGGCCG 1 cut(s) 483
Bsu15I ATCGAT 2 cut(s) 372, 777
BsuRI GGCC 5 cut(s) 71, 422, 485, 500, 506
BsuTUI ATCGAT 2 cut(s) 372, 777
BtgI CCRYGG 1 cut(s) 916
BtsCI GGATG 6 cut(s) 770, 1221, 1610, 1655, 1713, 1780
BtsIMutI CAGTG 1 cut(s) 2021
BveI ACCTGC 1 cut(s) 702
Cac8I GCNNGC 9 cut(s) 349, 651, 1020, 1655, 1719, 1798, 1811, 1860, 1960
CfoI GCGC 1 cut(s) 655
Cfr13I GGNCC 2 cut(s) 384, 421
ClaI ATCGAT 2 cut(s) 372, 777
CseI GACGC 2 cut(s) 277, 329
Csp6I GTAC 3 cut(s) 271, 391, 1447
CspCI CAANNNNNGTGG 2 cut(s) 1626, 1661
CviAII CATG 6 cut(s) 556, 806, 917, 1191, 1224, 1414
CviQI GTAC 3 cut(s) 271, 391, 1447
DdeI CTNAG 7 cut(s) 109, 1055, 1211, 1404, 1731, 1739, 2013
DriI GACNNNNNGTC 1 cut(s) 166
EaeI YGGCCR 2 cut(s) 483, 498
EagI CGGCCG 1 cut(s) 483
Eam1104I CTCTTC 1 cut(s) 40
Eam1105I GACNNNNNGTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 40
Ecl136II GAGCTC 2 cut(s) 472, 1438
EclXI CGGCCG 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 916
Eco24I GRGCYC 4 cut(s) 474, 1095, 1440, 1509
Eco47I GGWCC 1 cut(s) 384
Eco52I CGGCCG 1 cut(s) 483
Eco53kI GAGCTC 2 cut(s) 472, 1438
Eco88I CYCGRG 1 cut(s) 1508
EcoICRI GAGCTC 2 cut(s) 472, 1438
EcoRII CCWGG 5 cut(s) 132, 1072, 1230, 1501, 1905
EcoT14I CCWWGG 1 cut(s) 916
EcoT38I GRGCYC 4 cut(s) 474, 1095, 1440, 1509
ErhI CCWWGG 1 cut(s) 916
FaeI CATG 6 cut(s) 559, 809, 920, 1194, 1227, 1417
FaqI GGGAC 2 cut(s) 898, 1774
FatI CATG 6 cut(s) 555, 805, 916, 1190, 1223, 1413
FauI CCCGC 2 cut(s) 293, 1722
FokI GGATG 6 cut(s) 777, 1228, 1617, 1662, 1720, 1787
FriOI GRGCYC 4 cut(s) 474, 1095, 1440, 1509
FspBI CTAG 9 cut(s) 179, 203, 758, 882, 896, 1367, 1631, 1793, 1851
GlaI GCGC 1 cut(s) 654
GsaI CCCAGC 1 cut(s) 1707
GsuI CTGGAG 3 cut(s) 1321, 1339, 1889
HaeIII GGCC 5 cut(s) 71, 422, 485, 500, 506
HapII CCGG 5 cut(s) 268, 382, 399, 405, 1996
HgaI GACGC 2 cut(s) 277, 329
HhaI GCGC 1 cut(s) 655
Hin1II CATG 6 cut(s) 559, 809, 920, 1194, 1227, 1417
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 5 cut(s) 206, 479, 931, 1220, 1469
HpaII CCGG 5 cut(s) 268, 382, 399, 405, 1996
HphI GGTGA 5 cut(s) 136, 274, 641, 1090, 1132
Hpy166II GTNNAC 3 cut(s) 716, 1455, 1553
Hpy188I TCNGA 8 cut(s) 112, 244, 312, 476, 849, 1348, 1468, 1734
Hpy188III TCNNGA 9 cut(s) 58, 262, 399, 743, 758, 896, 1280, 1338, 1510
Hpy8I GTNNAC 3 cut(s) 716, 1455, 1553
Hpy99I CGWCG 3 cut(s) 293, 469, 724
HpyAV CCTTC 8 cut(s) 53, 181, 202, 528, 755, 1313, 1831, 1924
HpyCH4III ACNGT 5 cut(s) 720, 1532, 1591, 2031, 2049
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 491, 497, 826, 1419, 1640, 1806, 1815
HpyF3I CTNAG 7 cut(s) 109, 1055, 1211, 1404, 1731, 1739, 2013
Hsp92II CATG 6 cut(s) 559, 809, 920, 1194, 1227, 1417
HspAI GCGC 1 cut(s) 653
Kpn2I TCCGGA 1 cut(s) 398
KpnI GGTACC 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 6 cut(s) 245, 401, 469, 477, 1051, 1908
Lsp1109I GCAGC 7 cut(s) 475, 806, 1406, 1621, 1793, 1852, 1874
LweI GCATC 9 cut(s) 378, 748, 755, 783, 1259, 1640, 1690, 1695, 1809
MaeI CTAG 9 cut(s) 179, 203, 758, 882, 896, 1367, 1631, 1793, 1851
MaeIII GTNAC 7 cut(s) 280, 629, 732, 928, 1078, 1768, 1926
MbiI CCGCTC 1 cut(s) 240
MboII GAAGA 6 cut(s) 27, 88, 115, 662, 718, 2048
MfeI CAATTG 3 cut(s) 156, 1638, 1658
MflI RGATCY 3 cut(s) 667, 682, 1120
MhlI GDGCHC 5 cut(s) 474, 1095, 1440, 1457, 1509
MlyI GAGTC 3 cut(s) 473, 925, 1229
MmeI TCCRAC 4 cut(s) 267, 381, 2000, 2013
MroI TCCGGA 1 cut(s) 398
MseI TTAA 7 cut(s) 608, 1046, 1061, 1361, 1518, 1749, 1820
MslI CAYNNNNRTG 2 cut(s) 560, 1189
MspA1I CMGCKG 3 cut(s) 488, 1806, 1865
MspCI CTTAAG 1 cut(s) 1060
MspI CCGG 5 cut(s) 268, 382, 399, 405, 1996
MspR9I CCNGG 9 cut(s) 134, 268, 382, 406, 1074, 1232, 1503, 1907, 1997
MunI CAATTG 3 cut(s) 156, 1638, 1658
Mva1269I GAATGC 1 cut(s) 1247
MvaI CCWGG 5 cut(s) 134, 1074, 1232, 1503, 1907
MvnI CGCG 2 cut(s) 288, 1962
MwoI GCNNNNNNNGC 8 cut(s) 39, 491, 497, 826, 1419, 1640, 1806, 1815
NciI CCSGG 4 cut(s) 268, 382, 406, 1997
NcoI CCATGG 1 cut(s) 916
NlaIII CATG 6 cut(s) 559, 809, 920, 1194, 1227, 1417
NlaIV GGNNCC 4 cut(s) 272, 403, 617, 1236
NmeAIII GCCGAG 2 cut(s) 461, 526
NmuCI GTSAC 4 cut(s) 280, 629, 928, 1078
NspI RCATGY 1 cut(s) 1417
NspV TTCGAA 1 cut(s) 1944
PaeR7I CTCGAG 1 cut(s) 1508
PciSI GCTCTTC 1 cut(s) 40
PctI GAATGC 1 cut(s) 1247
PfeI GAWTC 2 cut(s) 206, 1469
PfoI TCCNGGA 1 cut(s) 132
PleI GAGTC 3 cut(s) 473, 925, 1228
PpsI GAGTC 3 cut(s) 473, 925, 1228
PshBI ATTAAT 2 cut(s) 1518, 1749
Psp124BI GAGCTC 2 cut(s) 474, 1440
Psp6I CCWGG 5 cut(s) 132, 1072, 1230, 1501, 1905
PspFI CCCAGC 1 cut(s) 1703
PspGI CCWGG 5 cut(s) 132, 1072, 1230, 1501, 1905
PspN4I GGNNCC 4 cut(s) 272, 403, 617, 1236
PspPI GGNCC 2 cut(s) 384, 421
PstI CTGCAG 1 cut(s) 1815
PsuI RGATCY 3 cut(s) 667, 682, 1120
PvuII CAGCTG 2 cut(s) 1806, 1865
RsaI GTAC 3 cut(s) 272, 392, 1448
RsaNI GTAC 3 cut(s) 271, 391, 1447
RseI CAYNNNNRTG 2 cut(s) 560, 1189
SacI GAGCTC 2 cut(s) 474, 1440
SapI GCTCTTC 1 cut(s) 40
SaqAI TTAA 7 cut(s) 608, 1046, 1061, 1361, 1518, 1749, 1820
Sau96I GGNCC 2 cut(s) 384, 421
ScaI AGTACT 1 cut(s) 392
SchI GAGTC 3 cut(s) 473, 925, 1229
ScrFI CCNGG 9 cut(s) 134, 268, 382, 406, 1074, 1232, 1503, 1907, 1997
SduI GDGCHC 5 cut(s) 474, 1095, 1440, 1457, 1509
SfaNI GCATC 9 cut(s) 378, 748, 755, 783, 1259, 1640, 1690, 1695, 1809
SfcI CTRYAG 2 cut(s) 410, 1811
Sfr274I CTCGAG 1 cut(s) 1508
SfuI TTCGAA 1 cut(s) 1944
SinI GGWCC 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 1508
SmiMI CAYNNNNRTG 2 cut(s) 560, 1189
SmlI CTYRAG 6 cut(s) 656, 743, 1060, 1280, 1508, 1576
SmoI CTYRAG 6 cut(s) 656, 743, 1060, 1280, 1508, 1576
SpeI ACTAGT 1 cut(s) 881
SspI AATATT 1 cut(s) 1693
SspMI CTAG 9 cut(s) 179, 203, 758, 882, 896, 1367, 1631, 1793, 1851
SstI GAGCTC 2 cut(s) 474, 1440
StyD4I CCNGG 9 cut(s) 132, 266, 380, 404, 1072, 1230, 1501, 1905, 1995
StyI CCWWGG 1 cut(s) 916
TaaI ACNGT 5 cut(s) 720, 1532, 1591, 2031, 2049
TatI WGTACW 2 cut(s) 390, 1446
TauI GCSGC 3 cut(s) 222, 488, 494
TfiI GAWTC 2 cut(s) 206, 1469
Tru1I TTAA 7 cut(s) 608, 1046, 1061, 1361, 1518, 1749, 1820
Tru9I TTAA 7 cut(s) 608, 1046, 1061, 1361, 1518, 1749, 1820
TscAI CASTG 1 cut(s) 2021
TseFI GTSAC 4 cut(s) 280, 629, 928, 1078
TseI GCWGC 7 cut(s) 488, 794, 1419, 1634, 1806, 1862, 1865
Tsp45I GTSAC 4 cut(s) 280, 629, 928, 1078
TspDTI ATGAA 4 cut(s) 115, 129, 1320, 1332
TspGWI ACGGA 3 cut(s) 482, 566, 1787
TspRI CASTG 1 cut(s) 2021
Vha464I CTTAAG 1 cut(s) 1060
VneI GTGCAC 1 cut(s) 1453
VpaK11BI GGWCC 1 cut(s) 384
VspI ATTAAT 2 cut(s) 1518, 1749
XapI RAATTY 3 cut(s) 1371, 1461, 1988
XbaI TCTAGA 2 cut(s) 757, 895
XceI RCATGY 1 cut(s) 1417
XhoI CTCGAG 1 cut(s) 1508
XspI CTAG 9 cut(s) 179, 203, 758, 882, 896, 1367, 1631, 1793, 1851
ZrmI AGTACT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.