Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0004828

Physical Location & Seq

Genomic Coordinates Reverse (-)

34285360 .. 34290510

Gene Structure 5151 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024302

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGAGAGAAAGGGCCCTTTTTATTGCGAGAGTGAGATCTGGGTGGGGGATAAGAGACCCTCGGGGTCTCACTGTTTCCGCGCTGCGGGTCCCGCTCTGGGCCCAACCCGGAAAATTTGAAAGATTTCTTATACAAGAGCAGCGCGATGAGTTCAAGCCTCTATTGCACATATTCAAGGAAGAGAGACCATCTCAGGCCACAGTAGATCGGATGCAGATAGAGAATACCACTGCTGAGGAAGATTTCAACATTGATGACTTTTACAAGTTGGTCAAAGACTTTCGCCAGTTGCATGTTTTACCCTTACAAACAGGTGAAAATTCATCCACCACTTGGGCTTCGAAGTCTAATACCATTGCTAATGAGCATATGGCTGTTGAGCCATGTGATGTTTTGTCCCTAGCTAAAAATGCTTTGTCGGCCTCAATAGAAGCAGCCGCTTTAGCTGAAAGCTCCAACTCGATCGGAGCTGGTTTTAATGAGTCTATTTCTATGGGTTTGGAGTGGGAAGAGGAAACTGTAAGGTCAACCAGACTTCTAGAGAGGCGGCGCAAGCGACTTAAGAAAAGGAATTTGCTTAACTCAAAAGTTCCAATTCATGAGACTAGCAGTTCTAGAAGTTCAGATGTACGGAGAAAATCTAGTGAAGGATTAAATCCTAATGATCCCCTGCGATTATTCTTGTGGGTACCTGAAACGAGACAACTTTTGACACTTAAAGAAGAGAATGAGTTGATTGCCCGAGTTCAGGATGTATTCAAGTTAGAAGCGGTGAAGAGTAGACTTCAATCTCAATATGGCCGTGAACCAACTTTGGTTGAATGGGCTGAAGCTGTTGGTATTAGTTGTAGGATGTTGCAATCACAACTTCGCTCTGGTACCAGCAGCCGTGAAAAGTTAATATATGCTAACTTACGTATGGTGGTGCACATTGCTAAACAATATCAAGGGCGAGGTCTTGGCCTTCAGGATTTACTGCAGGAGGGAAGTATGGGTCTAATGAAAAGTGTTGAGAAATTCAAACCCCAAGCTGGTTGCCGATTTGCTACTTATGCATACTGGTGGGTGAGACAAACAATTAGGAAGGCCATATTTCAACATTCCAGGACTATCCGCTTGCCGGAGAATGTATACACCCTCCTTGGCAAGGTATTGGAGGCAAAGAAATCATATATTCAGGAAGGAAATCACAACCCAAGCAGAGAAGAATTAGCAAGACGAGTTGGAATGTCGGTTGAAAAATTGGGAAAATTGCTATATTCTACGAGAATGCCTGTTTCAATGCAACAAACCGTGTGGGCAGACCAAGATACCACTTTCCAGGAGGTAACTGCTGACACTGGGATTGAGATCCCAGATGTGAGTGTGGCAAAGCAGCTGATGAGGCAACATGTGCGCAACCTCCTACACACTCTTGAAAAAAGAGAGAGGCAGATAATCAGGCTAAGATATGGTATTGAAGATGGTAAGCAGAGATCACTATCAGAGATTGGTGAGATTTTTGGATTGTCCAAGGAACGGGTACGGCAACTGGAGAGCCGAGCATTTCTGAAGCTCAAGGAATCACTTGGCAGCCAAGGGCTTGGGGCTTATGCTCCTTTGCTCGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

537

Amino Acids

61.41

Weight (kDa)

9.65

Isoelectric Point (pI)

51.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sigma70_r2	PF04542	301 - 370	3.5e-18	Sigma-70 region 2
Sigma70_r3	PF04539	380 - 452	9.9e-17	Sigma-70 region 3
Sigma70_r4	PF04545	468 - 521	6.8e-17	Sigma-70, region 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36990
fragaria_vesca	FvH4_6g16180 FvH4_6g16180 FvH4_6g16180 FvH4_6g16180 FvH4_6g16180 FvH4_6g16180
malus_domestica	MD12G1115000.v1.1
prunus_persica	Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1 Prupe.6G234100_v2.0.a1
pyrus_communis	pycom04g09010 pycom12g11420
rosa_chinensis	RchiOBHm_Chr3g0470031
rosa_laevigata	RLG00000024302
rosa_multiflora	Rmu_co8112822.1_g000001 Rmu_co8234937.1_g000001 Rmu_sc0017440.1_g000004 Rmu_sc0032107.1_g000001
rosa_roxburghii	Rroxscaffold_6G00411270
rosa_rugosa	Rorug03G0106100 Rorug03G0106100 Rorug03G0106200
rosa_samantha	Rh3AG155500 Rh3BG179600 Rh3CG170200 Rh3DG195500
rosa_wichuraiana	Rw3G014640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1397
Acc65I	GGTACC	2 cut(s)	688, 878
AccB1I	GGYRCC	2 cut(s)	688, 878
AccB7I	CCANNNNNTGG	1 cut(s)	333
AccBSI	CCGCTC	1 cut(s)	94
AccI	GTMKAC	2 cut(s)	781, 1131
AccII	CGCG	2 cut(s)	80, 144
AciI	CCGC	7 cut(s)	78, 85, 92, 438, 547, 770, 1114
AclWI	GGATC	2 cut(s)	659, 1345
AcoI	YGGCCR	1 cut(s)	799
AcsI	RAATTY	4 cut(s)	113, 319, 571, 1016
AcuI	CTGAAG	3 cut(s)	849, 950, 1571
AfaI	GTAC	4 cut(s)	630, 690, 880, 1524
AfiI	CCNNNNNNNGG	8 cut(s)	84, 97, 333, 748, 1031, 1120, 1147, 1518
AflII	CTTAAG	1 cut(s)	560
AflIII	ACRYGT	1 cut(s)	1390
AjnI	CCWGG	2 cut(s)	1103, 1320
AluBI	AGCT	8 cut(s)	404, 446, 453, 470, 833, 1031, 1378, 1555
AluI	AGCT	8 cut(s)	404, 446, 453, 470, 833, 1031, 1378, 1555
Alw21I	GWGCWC	1 cut(s)	930
Alw26I	GTCTC	6 cut(s)	48, 71, 178, 596, 694, 1063
Alw44I	GTGCAC	1 cut(s)	926
AlwI	GGATC	2 cut(s)	659, 1345
Ama87I	CYCGRG	2 cut(s)	60, 741
AoxI	GGCC	7 cut(s)	12, 99, 195, 420, 799, 961, 1086
ApaI	GGGCCC	2 cut(s)	16, 103
ApaLI	GTGCAC	1 cut(s)	926
ApeKI	GCWGC	6 cut(s)	82, 139, 434, 885, 1375, 1572
ApoI	RAATTY	4 cut(s)	113, 319, 571, 1016
Asp700I	GAANNNNTTC	1 cut(s)	123
Asp718I	GGTACC	2 cut(s)	688, 878
AspLEI	GCGC	4 cut(s)	82, 144, 552, 1398
AspS9I	GGNCC	5 cut(s)	12, 13, 88, 99, 100
AsuC2I	CCSGG	1 cut(s)	108
AsuHPI	GGTGA	4 cut(s)	326, 784, 1078, 1505
AsuII	TTCGAA	1 cut(s)	341
AvaI	CYCGRG	2 cut(s)	60, 741
AvaII	GGWCC	1 cut(s)	88
BaeGI	GKGCMC	3 cut(s)	16, 103, 930
BanI	GGYRCC	2 cut(s)	688, 878
BanII	GRGCYC	2 cut(s)	16, 103
Bbv12I	GWGCWC	1 cut(s)	930
BbvCI	CCTCAGC	1 cut(s)	234
BbvI	GCAGC	6 cut(s)	69, 151, 446, 897, 1387, 1584
BccI	CCATC	2 cut(s)	196, 1457
BceAI	ACGGC	3 cut(s)	786, 873, 1541
BciT130I	CCWGG	2 cut(s)	1105, 1322
BcnI	CCSGG	1 cut(s)	108
BcoDI	GTCTC	6 cut(s)	48, 71, 178, 596, 694, 1063
BfaI	CTAG	5 cut(s)	401, 539, 606, 615, 642
BfmI	CTRYAG	1 cut(s)	977
BfrI	CTTAAG	1 cut(s)	560
BglII	AGATCT	1 cut(s)	35
BisI	GCNGC	8 cut(s)	83, 140, 435, 438, 548, 886, 1376, 1573
BlsI	GCNGC	8 cut(s)	84, 141, 436, 439, 549, 887, 1377, 1574
Bme1390I	CCNGG	3 cut(s)	108, 1105, 1322
Bme18I	GGWCC	1 cut(s)	88
BmeT110I	CYCGRG	2 cut(s)	60, 741
BmgT120I	GGNCC	5 cut(s)	12, 13, 88, 99, 100
BmiI	GGNNCC	6 cut(s)	14, 89, 90, 101, 690, 880
BmrFI	CCNGG	3 cut(s)	108, 1105, 1322
BmrI	ACTGGG	1 cut(s)	1350
BmsI	GCATC	1 cut(s)	201
BmuI	ACTGGG	1 cut(s)	1350
BplI	GAGNNNNNCTC	2 cut(s)	175, 207
BpmI	CTGGAG	1 cut(s)	1553
Bpu10I	CCTNAGC	1 cut(s)	234
Bpu14I	TTCGAA	1 cut(s)	341
BpuEI	CTTGAG	1 cut(s)	1541
BpuMI	CCSGG	1 cut(s)	108
BsaAI	YACGTR	1 cut(s)	917
BsaBI	GATNNNNATC	2 cut(s)	1349, 1480
BsaI	GGTCTC	3 cut(s)	48, 71, 178
BsaJI	CCNNGG	4 cut(s)	59, 1141, 1512, 1576
Bsc4I	CCNNNNNNNGG	8 cut(s)	84, 97, 333, 748, 1031, 1120, 1147, 1518
Bse1I	ACTGG	4 cut(s)	286, 1064, 1345, 1536
Bse3DI	GCAATG	2 cut(s)	354, 930
Bse8I	GATNNNNATC	2 cut(s)	1349, 1480
BseBI	CCWGG	2 cut(s)	1105, 1322
BseDI	CCNNGG	4 cut(s)	59, 1141, 1512, 1576
BseGI	GGATG	4 cut(s)	216, 323, 757, 858
BseJI	GATNNNNATC	2 cut(s)	1349, 1480
BseLI	CCNNNNNNNGG	8 cut(s)	84, 97, 333, 748, 1031, 1120, 1147, 1518
BseMI	GCAATG	2 cut(s)	354, 930
BseMII	CTCAG	2 cut(s)	206, 225
BseNI	ACTGG	4 cut(s)	286, 1064, 1345, 1536
BseSI	GKGCMC	3 cut(s)	16, 103, 930
BseXI	GCAGC	6 cut(s)	69, 151, 446, 897, 1387, 1584
Bsh1236I	CGCG	2 cut(s)	80, 144
Bsh1285I	CGRYCG	1 cut(s)	465
BshFI	GGCC	7 cut(s)	14, 101, 197, 422, 801, 963, 1088
BshNI	GGYRCC	2 cut(s)	688, 878
BsiEI	CGRYCG	1 cut(s)	465
BsiHKAI	GWGCWC	1 cut(s)	930
BsiHKCI	CYCGRG	2 cut(s)	60, 741
BsiSI	CCGG	2 cut(s)	108, 1121
BslFI	GGGAC	2 cut(s)	74, 382
BslI	CCNNNNNNNGG	8 cut(s)	84, 97, 333, 748, 1031, 1120, 1147, 1518
BsmAI	GTCTC	6 cut(s)	48, 71, 178, 596, 694, 1063
BsmFI	GGGAC	2 cut(s)	74, 382
BsmI	GAATGC	1 cut(s)	1275
BsnI	GGCC	7 cut(s)	14, 101, 197, 422, 801, 963, 1088
Bso31I	GGTCTC	3 cut(s)	48, 71, 178
BsoBI	CYCGRG	2 cut(s)	60, 741
Bsp119I	TTCGAA	1 cut(s)	341
Bsp120I	GGGCCC	2 cut(s)	12, 99
Bsp1286I	GDGCHC	3 cut(s)	16, 103, 930
Bsp143I	GATC	6 cut(s)	35, 205, 462, 664, 1350, 1475
BspACI	CCGC	7 cut(s)	78, 85, 92, 438, 547, 770, 1114
BspANI	GGCC	7 cut(s)	14, 101, 197, 422, 801, 963, 1088
BspCNI	CTCAG	2 cut(s)	205, 226
BspFNI	CGCG	2 cut(s)	80, 144
BspHI	TCATGA	1 cut(s)	598
BspLI	GGNNCC	6 cut(s)	14, 89, 90, 101, 690, 880
BspMAI	CTGCAG	1 cut(s)	981
BspPI	GGATC	2 cut(s)	659, 1345
BspT104I	TTCGAA	1 cut(s)	341
BspT107I	GGYRCC	2 cut(s)	688, 878
BspTI	CTTAAG	1 cut(s)	560
BspTNI	GGTCTC	3 cut(s)	48, 71, 178
BsrBI	CCGCTC	1 cut(s)	94
BsrDI	GCAATG	2 cut(s)	354, 930
BsrI	ACTGG	4 cut(s)	286, 1064, 1345, 1536
BssECI	CCNNGG	4 cut(s)	59, 1141, 1512, 1576
BssMI	GATC	6 cut(s)	35, 205, 462, 664, 1350, 1475
BssNAI	GTATAC	1 cut(s)	1132
BssT1I	CCWWGG	3 cut(s)	1141, 1512, 1576
Bst1107I	GTATAC	1 cut(s)	1132
Bst2UI	CCWGG	2 cut(s)	1105, 1322
Bst4CI	ACNGT	4 cut(s)	73, 202, 520, 1294
Bst6I	CTCTTC	4 cut(s)	174, 504, 717, 770
BstAFI	CTTAAG	1 cut(s)	560
BstAPI	GCANNNNNTGC	1 cut(s)	1393
BstBAI	YACGTR	1 cut(s)	917
BstBI	TTCGAA	1 cut(s)	341
BstC8I	GCNNGC	2 cut(s)	554, 1118
BstDEI	CTNAG	3 cut(s)	192, 234, 1445
BstENI	CCTNNNNNAGG	1 cut(s)	1145
BstF5I	GGATG	4 cut(s)	216, 323, 757, 858
BstFNI	CGCG	2 cut(s)	80, 144
BstHHI	GCGC	4 cut(s)	82, 144, 552, 1398
BstKTI	GATC	6 cut(s)	38, 208, 465, 667, 1353, 1478
BstMAI	GTCTC	6 cut(s)	48, 71, 178, 596, 694, 1063
BstMBI	GATC	6 cut(s)	35, 205, 462, 664, 1350, 1475
BstMCI	CGRYCG	1 cut(s)	465
BstMWI	GCNNNNNNNGC	9 cut(s)	91, 163, 410, 419, 443, 553, 1052, 1384, 1393
BstNI	CCWGG	2 cut(s)	1105, 1322
BstNSI	RCATGY	2 cut(s)	296, 1394
BstSCI	CCNGG	3 cut(s)	106, 1103, 1320
BstSFI	CTRYAG	1 cut(s)	977
BstSLI	GKGCMC	3 cut(s)	16, 103, 930
BstSNI	TACGTA	1 cut(s)	917
BstUI	CGCG	2 cut(s)	80, 144
BstV1I	GCAGC	6 cut(s)	69, 151, 446, 897, 1387, 1584
BstX2I	RGATCY	2 cut(s)	35, 1350
BstXI	CCANNNNNNTGG	1 cut(s)	1583
BstYI	RGATCY	2 cut(s)	35, 1350
BstZ17I	GTATAC	1 cut(s)	1132
BsuRI	GGCC	7 cut(s)	14, 101, 197, 422, 801, 963, 1088
BtgZI	GCGATG	1 cut(s)	159
BtsCI	GGATG	4 cut(s)	216, 323, 757, 858
BtsI	GCAGTG	1 cut(s)	228
BtsIMutI	CAGTG	3 cut(s)	69, 228, 1338
Cac8I	GCNNGC	2 cut(s)	554, 1118
CciI	TCATGA	1 cut(s)	598
CfoI	GCGC	4 cut(s)	82, 144, 552, 1398
Cfr13I	GGNCC	5 cut(s)	12, 13, 88, 99, 100
Csp6I	GTAC	4 cut(s)	629, 689, 879, 1523
CspCI	CAANNNNNGTGG	2 cut(s)	1277, 1312
CviAII	CATG	4 cut(s)	293, 384, 599, 1391
CviQI	GTAC	4 cut(s)	629, 689, 879, 1523
DdeI	CTNAG	3 cut(s)	192, 234, 1445
DpnI	GATC	6 cut(s)	37, 207, 464, 666, 1352, 1477
DpnII	GATC	6 cut(s)	35, 205, 462, 664, 1350, 1475
EaeI	YGGCCR	1 cut(s)	799
Eam1104I	CTCTTC	4 cut(s)	174, 504, 717, 770
EarI	CTCTTC	4 cut(s)	174, 504, 717, 770
Eco105I	TACGTA	1 cut(s)	917
Eco130I	CCWWGG	3 cut(s)	1141, 1512, 1576
Eco24I	GRGCYC	2 cut(s)	16, 103
Eco31I	GGTCTC	3 cut(s)	48, 71, 178
Eco47I	GGWCC	1 cut(s)	88
Eco57I	CTGAAG	3 cut(s)	849, 950, 1571
Eco88I	CYCGRG	2 cut(s)	60, 741
EcoNI	CCTNNNNNAGG	1 cut(s)	1145
EcoO109I	RGGNCCY	3 cut(s)	12, 13, 88
EcoRII	CCWGG	2 cut(s)	1103, 1320
EcoT14I	CCWWGG	3 cut(s)	1141, 1512, 1576
EcoT22I	ATGCAT	1 cut(s)	1057
EcoT38I	GRGCYC	2 cut(s)	16, 103
ErhI	CCWWGG	3 cut(s)	1141, 1512, 1576
FaeI	CATG	4 cut(s)	296, 387, 602, 1394
FaqI	GGGAC	2 cut(s)	74, 382
FatI	CATG	4 cut(s)	292, 383, 598, 1390
FauI	CCCGC	2 cut(s)	78, 99
FauNDI	CATATG	1 cut(s)	369
FblI	GTMKAC	2 cut(s)	781, 1131
Fnu4HI	GCNGC	8 cut(s)	83, 140, 435, 438, 548, 886, 1376, 1573
FokI	GGATG	4 cut(s)	223, 310, 764, 865
FriOI	GRGCYC	2 cut(s)	16, 103
Fsp4HI	GCNGC	8 cut(s)	83, 140, 435, 438, 548, 886, 1376, 1573
FspBI	CTAG	5 cut(s)	401, 539, 606, 615, 642
FspI	TGCGCA	1 cut(s)	1397
GlaI	GCGC	4 cut(s)	81, 143, 551, 1397
GluI	GCNGC	8 cut(s)	83, 140, 435, 438, 548, 886, 1376, 1573
GsuI	CTGGAG	1 cut(s)	1553
HaeIII	GGCC	7 cut(s)	14, 101, 197, 422, 801, 963, 1088
HapII	CCGG	2 cut(s)	108, 1121
HhaI	GCGC	4 cut(s)	82, 144, 552, 1398
Hin1II	CATG	4 cut(s)	296, 387, 602, 1394
Hin6I	GCGC	4 cut(s)	80, 142, 550, 1396
HinP1I	GCGC	4 cut(s)	80, 142, 550, 1396
HincII	GTYRAC	1 cut(s)	528
HindII	GTYRAC	1 cut(s)	528
HinfI	GANTC	2 cut(s)	482, 1562
HpaII	CCGG	2 cut(s)	108, 1121
HphI	GGTGA	4 cut(s)	326, 784, 1078, 1505
Hpy166II	GTNNAC	5 cut(s)	528, 782, 806, 928, 1132
Hpy188I	TCNGA	5 cut(s)	210, 467, 625, 1486, 1551
Hpy188III	TCNNGA	7 cut(s)	539, 599, 615, 749, 968, 1178, 1415
Hpy8I	GTNNAC	5 cut(s)	528, 782, 806, 928, 1132
HpyAV	CCTTC	4 cut(s)	641, 974, 1078, 1175
HpyCH4III	ACNGT	4 cut(s)	73, 202, 520, 1294
HpyCH4IV	ACGT	1 cut(s)	916
HpyCH4V	TGCA	8 cut(s)	166, 214, 292, 859, 928, 979, 1055, 1285
HpyF10VI	GCNNNNNNNGC	9 cut(s)	91, 163, 410, 419, 443, 553, 1052, 1384, 1393
HpyF3I	CTNAG	3 cut(s)	192, 234, 1445
HpySE526I	ACGT	1 cut(s)	916
Hsp92II	CATG	4 cut(s)	296, 387, 602, 1394
HspAI	GCGC	4 cut(s)	80, 142, 550, 1396
KflI	GGGWCCC	1 cut(s)	88
KpnI	GGTACC	2 cut(s)	692, 882
Kzo9I	GATC	6 cut(s)	35, 205, 462, 664, 1350, 1475
LmnI	GCTCC	3 cut(s)	458, 467, 1600
Lsp1109I	GCAGC	6 cut(s)	69, 151, 446, 897, 1387, 1584
LweI	GCATC	1 cut(s)	201
MaeI	CTAG	5 cut(s)	401, 539, 606, 615, 642
MaeII	ACGT	1 cut(s)	916
MaeIII	GTNAC	1 cut(s)	1327
MalI	GATC	6 cut(s)	37, 207, 464, 666, 1352, 1477
MbiI	CCGCTC	1 cut(s)	94
MboI	GATC	6 cut(s)	35, 205, 462, 664, 1350, 1475
MboII	GAAGA	7 cut(s)	191, 251, 521, 734, 787, 1217, 1472
MflI	RGATCY	2 cut(s)	35, 1350
MhlI	GDGCHC	3 cut(s)	16, 103, 930
MluCI	AATT	9 cut(s)	113, 319, 571, 594, 1016, 1077, 1208, 1241, 1250
MlyI	GAGTC	1 cut(s)	491
MmeI	TCCRAC	2 cut(s)	480, 1204
Mph1103I	ATGCAT	1 cut(s)	1057
MroXI	GAANNNNTTC	1 cut(s)	123
MseI	TTAA	7 cut(s)	477, 561, 579, 653, 717, 899, 1609
MslI	CAYNNNNRTG	1 cut(s)	1060
MspA1I	CMGCKG	1 cut(s)	1378
MspCI	CTTAAG	1 cut(s)	560
MspI	CCGG	2 cut(s)	108, 1121
MspR9I	CCNGG	3 cut(s)	108, 1105, 1322
Mva1269I	GAATGC	1 cut(s)	1275
MvaI	CCWGG	2 cut(s)	1105, 1322
MvnI	CGCG	2 cut(s)	80, 144
MwoI	GCNNNNNNNGC	9 cut(s)	91, 163, 410, 419, 443, 553, 1052, 1384, 1393
NciI	CCSGG	1 cut(s)	108
NdeI	CATATG	1 cut(s)	369
NdeII	GATC	6 cut(s)	35, 205, 462, 664, 1350, 1475
NlaIII	CATG	4 cut(s)	296, 387, 602, 1394
NlaIV	GGNNCC	6 cut(s)	14, 89, 90, 101, 690, 880
NmeAIII	GCCGAG	1 cut(s)	1565
NsbI	TGCGCA	1 cut(s)	1397
NsiI	ATGCAT	1 cut(s)	1057
NspI	RCATGY	2 cut(s)	296, 1394
NspV	TTCGAA	1 cut(s)	341
PagI	TCATGA	1 cut(s)	598
PciI	ACATGT	1 cut(s)	1390
PctI	GAATGC	1 cut(s)	1275
PdmI	GAANNNNTTC	1 cut(s)	123
PfeI	GAWTC	1 cut(s)	1562
PflMI	CCANNNNNTGG	1 cut(s)	333
PfoI	TCCNGGA	2 cut(s)	1103, 1320
PkrI	GCNGC	8 cut(s)	84, 141, 436, 439, 549, 887, 1377, 1574
Ple19I	CGATCG	1 cut(s)	465
PleI	GAGTC	1 cut(s)	490
PpsI	GAGTC	1 cut(s)	490
Ppu21I	YACGTR	1 cut(s)	917
PpuMI	RGGWCCY	1 cut(s)	88
PscI	ACATGT	1 cut(s)	1390
Psp5II	RGGWCCY	1 cut(s)	88
Psp6I	CCWGG	2 cut(s)	1103, 1320
PspGI	CCWGG	2 cut(s)	1103, 1320
PspN4I	GGNNCC	6 cut(s)	14, 89, 90, 101, 690, 880
PspOMI	GGGCCC	2 cut(s)	12, 99
PspPI	GGNCC	5 cut(s)	12, 13, 88, 99, 100
PspPPI	RGGWCCY	1 cut(s)	88
PstI	CTGCAG	1 cut(s)	981
PsuI	RGATCY	2 cut(s)	35, 1350
PvuI	CGATCG	1 cut(s)	465
PvuII	CAGCTG	1 cut(s)	1378
RsaI	GTAC	4 cut(s)	630, 690, 880, 1524
RsaNI	GTAC	4 cut(s)	629, 689, 879, 1523
RseI	CAYNNNNRTG	1 cut(s)	1060
SaqAI	TTAA	7 cut(s)	477, 561, 579, 653, 717, 899, 1609
SatI	GCNGC	8 cut(s)	83, 140, 435, 438, 548, 886, 1376, 1573
Sau3AI	GATC	6 cut(s)	35, 205, 462, 664, 1350, 1475
Sau96I	GGNCC	5 cut(s)	12, 13, 88, 99, 100
SchI	GAGTC	1 cut(s)	491
ScrFI	CCNGG	3 cut(s)	108, 1105, 1322
SduI	GDGCHC	3 cut(s)	16, 103, 930
SfaNI	GCATC	1 cut(s)	201
SfcI	CTRYAG	1 cut(s)	977
SfuI	TTCGAA	1 cut(s)	341
SinI	GGWCC	1 cut(s)	88
SmiMI	CAYNNNNRTG	1 cut(s)	1060
SmlI	CTYRAG	2 cut(s)	560, 1556
SmoI	CTYRAG	2 cut(s)	560, 1556
SnaBI	TACGTA	1 cut(s)	917
Sse9I	AATT	9 cut(s)	113, 319, 571, 594, 1016, 1077, 1208, 1241, 1250
SsiI	CCGC	7 cut(s)	78, 85, 92, 438, 547, 770, 1114
SspMI	CTAG	5 cut(s)	401, 539, 606, 615, 642
StyD4I	CCNGG	3 cut(s)	106, 1103, 1320
StyI	CCWWGG	3 cut(s)	1141, 1512, 1576
TaaI	ACNGT	4 cut(s)	73, 202, 520, 1294
TaiI	ACGT	1 cut(s)	919
TaqI	TCGA	2 cut(s)	341, 461
TasI	AATT	9 cut(s)	113, 319, 571, 594, 1016, 1077, 1208, 1241, 1250
TauI	GCSGC	2 cut(s)	440, 550
TfiI	GAWTC	1 cut(s)	1562
Tru1I	TTAA	7 cut(s)	477, 561, 579, 653, 717, 899, 1609
Tru9I	TTAA	7 cut(s)	477, 561, 579, 653, 717, 899, 1609
TscAI	CASTG	3 cut(s)	76, 235, 1345
TseI	GCWGC	6 cut(s)	82, 139, 434, 885, 1375, 1572
TspDTI	ATGAA	3 cut(s)	312, 587, 1016
TspGWI	ACGGA	1 cut(s)	646
TspRI	CASTG	3 cut(s)	76, 235, 1345
Van91I	CCANNNNNTGG	1 cut(s)	333
Vha464I	CTTAAG	1 cut(s)	560
VneI	GTGCAC	1 cut(s)	926
VpaK11BI	GGWCC	1 cut(s)	88
XagI	CCTNNNNNAGG	1 cut(s)	1145
XapI	RAATTY	4 cut(s)	113, 319, 571, 1016
XbaI	TCTAGA	2 cut(s)	538, 614
XceI	RCATGY	2 cut(s)	296, 1394
XmiI	GTMKAC	2 cut(s)	781, 1131
XmnI	GAANNNNTTC	1 cut(s)	123
XspI	CTAG	5 cut(s)	401, 539, 606, 615, 642
Zsp2I	ATGCAT	1 cut(s)	1057

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.