RchiOBHm_Chr4g0393221

NHL repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0018604
Physical Location & Seq
Forward (+)
8726906 .. 8732114
5209 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36585

Sequence Viewer

Length: 411 bp
ATGAGGGAGATGAGTTGGTTGAAATTAACGGTAGTCAATGATGGAGATATGTATCTATGGAGAGAGCTAGGTGTGAATTCATGGCTAATATTTGCTGTTGTTGGGCCCAATGGAAGACTTCTTGCTCAACATTTGGAGAAAGATAATGATCCCCGTTTGTTTACATCTCCATTAAAGTTTCCTGGAAAGCTAGCAGTTGATGTCGAAAACAATAGGCTCTTCATTTCAGACAGTAATCATATCCGCATACTACTCAACTCTCCATGGGATGTCTGCTTTCATCTAGTCAATGAGAAAGTTTATATTGCCATGGCTGGCCAACATCAAATTTGGCAACTTGATACAGCTGATGGGATTACTAGAGCATTTAGTGGTGATGGTTATGAAAGAAATCTGAATGGATCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 NHL Thioredoxin_8

Protein Analysis

136

Amino Acids

15.6

Weight (kDa)

6.05

Isoelectric Point (pI)

22.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 244
AclWI GGATC 2 cut(s) 143, 409
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 2 cut(s) 76, 327
AgsI TTSAA 1 cut(s) 22
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 3 cut(s) 67, 190, 347
AluI AGCT 3 cut(s) 67, 190, 347
AlwI GGATC 2 cut(s) 143, 409
AoxI GGCC 2 cut(s) 104, 316
ApaI GGGCCC 1 cut(s) 108
ApoI RAATTY 2 cut(s) 76, 327
AspS9I GGNCC 2 cut(s) 104, 105
AsuHPI GGTGA 1 cut(s) 386
AsuNHI GCTAGC 1 cut(s) 190
BaeGI GKGCMC 1 cut(s) 108
BalI TGGCCA 1 cut(s) 318
BanII GRGCYC 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 121
BccI CCATC 3 cut(s) 35, 344, 371
BciT130I CCWGG 1 cut(s) 183
BfaI CTAG 4 cut(s) 68, 191, 284, 360
Bme1390I CCNGG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 104, 105
BmiI GGNNCC 1 cut(s) 106
BmrFI CCNGG 1 cut(s) 183
BmtI GCTAGC 1 cut(s) 194
BpiI GAAGAC 1 cut(s) 121
BsaBI GATNNNNATC 2 cut(s) 51, 147
BsaJI CCNNGG 2 cut(s) 263, 309
Bse8I GATNNNNATC 2 cut(s) 51, 147
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 2 cut(s) 263, 309
BseGI GGATG 1 cut(s) 274
BseJI GATNNNNATC 2 cut(s) 51, 147
BseSI GKGCMC 1 cut(s) 108
BshFI GGCC 2 cut(s) 106, 318
BsnI GGCC 2 cut(s) 106, 318
Bsp120I GGGCCC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 148, 401
Bsp19I CCATGG 2 cut(s) 263, 309
BspACI CCGC 1 cut(s) 244
BspANI GGCC 2 cut(s) 106, 318
BspLI GGNNCC 1 cut(s) 106
BspOI GCTAGC 1 cut(s) 194
BspPI GGATC 2 cut(s) 143, 409
BspQI GCTCTTC 1 cut(s) 224
BssECI CCNNGG 2 cut(s) 263, 309
BssMI GATC 2 cut(s) 148, 401
BssT1I CCWWGG 2 cut(s) 263, 309
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 31, 233
Bst6I CTCTTC 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 192, 316
BstDSI CCRYGG 2 cut(s) 263, 309
BstF5I GGATG 1 cut(s) 274
BstKTI GATC 2 cut(s) 151, 404
BstMBI GATC 2 cut(s) 148, 401
BstNI CCWGG 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 121
BsuRI GGCC 2 cut(s) 106, 318
BtgI CCRYGG 2 cut(s) 263, 309
BtsCI GGATG 1 cut(s) 274
Cac8I GCNNGC 2 cut(s) 192, 316
Cfr13I GGNCC 2 cut(s) 104, 105
CviAII CATG 3 cut(s) 81, 264, 310
CviJI RGCY 8 cut(s) 67, 85, 106, 190, 217, 314, 318, 347
CviKI_1 RGCY 8 cut(s) 67, 85, 106, 190, 217, 314, 318, 347
DpnI GATC 2 cut(s) 150, 403
DpnII GATC 2 cut(s) 148, 401
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Eco130I CCWWGG 2 cut(s) 263, 309
Eco24I GRGCYC 1 cut(s) 108
EcoRI GAATTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 181
EcoT14I CCWWGG 2 cut(s) 263, 309
EcoT38I GRGCYC 1 cut(s) 108
ErhI CCWWGG 2 cut(s) 263, 309
FaeI CATG 3 cut(s) 84, 267, 313
FaiI YATR 9 cut(s) 50, 58, 82, 240, 248, 265, 303, 311, 384
FatI CATG 3 cut(s) 80, 263, 309
FokI GGATG 1 cut(s) 281
FriOI GRGCYC 1 cut(s) 108
FspBI CTAG 4 cut(s) 68, 191, 284, 360
HaeIII GGCC 2 cut(s) 106, 318
Hin1II CATG 3 cut(s) 84, 267, 313
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 2 cut(s) 229, 396
Hpy8I GTNNAC 1 cut(s) 162
HpyCH4III ACNGT 2 cut(s) 31, 233
Hsp92II CATG 3 cut(s) 84, 267, 313
Kzo9I GATC 2 cut(s) 148, 401
LguI GCTCTTC 1 cut(s) 224
LpnPI CCDG 3 cut(s) 168, 195, 300
MaeI CTAG 4 cut(s) 68, 191, 284, 360
MalI GATC 2 cut(s) 150, 403
MboI GATC 2 cut(s) 148, 401
MboII GAAGA 2 cut(s) 126, 211
MhlI GDGCHC 1 cut(s) 108
MlsI TGGCCA 1 cut(s) 318
MluCI AATT 3 cut(s) 23, 76, 327
MluNI TGGCCA 1 cut(s) 318
Mox20I TGGCCA 1 cut(s) 318
MscI TGGCCA 1 cut(s) 318
MseI TTAA 2 cut(s) 26, 173
Msp20I TGGCCA 1 cut(s) 318
MspA1I CMGCKG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 183
NcoI CCATGG 2 cut(s) 263, 309
NdeII GATC 2 cut(s) 148, 401
NheI GCTAGC 1 cut(s) 190
NlaIII CATG 3 cut(s) 84, 267, 313
NlaIV GGNNCC 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 224
PfoI TCCNGGA 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 106
PspOMI GGGCCC 1 cut(s) 104
PspPI GGNCC 2 cut(s) 104, 105
PvuII CAGCTG 1 cut(s) 347
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 26, 173
Sau3AI GATC 2 cut(s) 148, 401
Sau96I GGNCC 2 cut(s) 104, 105
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 1 cut(s) 108
SetI ASST 5 cut(s) 69, 73, 192, 349, 410
Sse9I AATT 3 cut(s) 23, 76, 327
SsiI CCGC 1 cut(s) 244
SspI AATATT 1 cut(s) 90
SspMI CTAG 4 cut(s) 68, 191, 284, 360
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 2 cut(s) 263, 309
TaaI ACNGT 2 cut(s) 31, 233
TaqI TCGA 1 cut(s) 204
TasI AATT 3 cut(s) 23, 76, 327
Tru1I TTAA 2 cut(s) 26, 173
Tru9I TTAA 2 cut(s) 26, 173
TspDTI ATGAA 4 cut(s) 69, 211, 269, 399
XapI RAATTY 2 cut(s) 76, 327
XspI CTAG 4 cut(s) 68, 191, 284, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.