RchiOBHm_Chr4g0442261

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0018599
Physical Location & Seq
Reverse (-)
63914229 .. 63914933
705 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41003

Sequence Viewer

Length: 705 bp
ATGATTCCAGGTCCCTTTGCTAGGGAGGTTTTAACCATAATCAAGTCTCTACAAGACAAGCTCGTTAAAATTGGGAAAGCAGATATGGTGACAACATCTGTGGGATCATCAGTTTTGGGGGCTTCTTATCCGCCTTCTACCGGTGAGTTTGCAGCAAATAACCTCAATGACATGAAAGACGTTCTCCGGCTTCTATCAGCATACGGAGCACCGCTGATGATCAATGTCTATCCATACTATGCATATGCTGCCGACCGAAGCCACGTTTCCTTGGACTACGCTCTCTTTAATGCAACCGCCCCTGTGGTTCATGATGGGGAATTGGCCTACCAGAACCTTTTCGATGCTATGGTGGATTCATTCTACTGGGCAATGGAGAAAGCAGAGTACCGAAATGTGAGTATCGTGATGTCTGAGACTGGCTGGCCTCGCACTGGCACTATCCCAGAAGTTGCAGAAACATATAGCCGGAACTTGGTGAAACGGATGAAGTCAGGAGTGGGGACCCCCAAAAGACCTAACGATACTTTTGGAGCGTTTATATATGCGTACCACGATGGGCCACATGATTCTTTTGGAGTTTTCGATCCAAACCACACCGACCGGCCTTTTTATGCATTTCCAAGGCGGGAGGCCCCTGTATTACCGTTGTGTTATAATAAAGTACTTTTGCAATCTTACGTAAGATTGCAAAAAGATAAATAA

Protein Analysis

234

Amino Acids

26.18

Weight (kDa)

6.97

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 29 - 199 2.1e-39 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018599)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0442261
rosa_laevigata RLG00000006021
rosa_multiflora Rmu_sc0000124.1_g000058
rosa_roxburghii Rroxscaffold_5G00383320
rosa_rugosa Rorug04G0340100
rosa_samantha Rh4AG391700 Rh4AG393200 Rh4CG420400 Rh4DG398500 Rh4DG400200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AciI CCGC 4 cut(s) 131, 212, 297, 628
AclWI GGATC 2 cut(s) 112, 581
AfaI GTAC 3 cut(s) 389, 551, 666
AfiI CCNNNNNNNGG 4 cut(s) 21, 140, 434, 475
AgeI ACCGGT 1 cut(s) 140
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 1 cut(s) 61
AluI AGCT 1 cut(s) 61
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 2 cut(s) 51, 410
AlwI GGATC 2 cut(s) 112, 581
AoxI GGCC 5 cut(s) 324, 425, 560, 605, 633
ApeKI GCWGC 2 cut(s) 152, 248
AsiGI ACCGGT 1 cut(s) 140
Asp700I GAANNNNTTC 1 cut(s) 338
AspS9I GGNCC 4 cut(s) 11, 504, 560, 634
AsuHPI GGTGA 3 cut(s) 100, 155, 490
AvaII GGWCC 2 cut(s) 11, 504
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 164, 235
BccI CCATC 2 cut(s) 308, 551
BciT130I CCWGG 1 cut(s) 9
BclI TGATCA 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 51, 410
BfaI CTAG 1 cut(s) 21
BisI GCNGC 2 cut(s) 153, 249
BlsI GCNGC 2 cut(s) 154, 250
BmcAI AGTACT 1 cut(s) 666
Bme1390I CCNGG 1 cut(s) 9
Bme18I GGWCC 2 cut(s) 11, 504
BmgT120I GGNCC 4 cut(s) 11, 504, 560, 634
BmiI GGNNCC 4 cut(s) 13, 505, 506, 636
BmrFI CCNGG 1 cut(s) 9
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 1 cut(s) 334
BmuI ACTGGG 1 cut(s) 376
BsaAI YACGTR 1 cut(s) 682
BsaJI CCNNGG 2 cut(s) 270, 623
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 140, 434, 475
Bse118I RCCGGY 2 cut(s) 140, 603
Bse1I ACTGG 3 cut(s) 371, 424, 439
Bse3DI GCAATG 1 cut(s) 378
BseBI CCWGG 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 270, 623
BseGI GGATG 1 cut(s) 492
BseLI CCNNNNNNNGG 4 cut(s) 21, 140, 434, 475
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 3 cut(s) 371, 424, 439
BseXI GCAGC 2 cut(s) 164, 235
Bsh1285I CGRYCG 2 cut(s) 256, 604
BshFI GGCC 5 cut(s) 326, 427, 562, 607, 635
BshTI ACCGGT 1 cut(s) 140
BsiEI CGRYCG 2 cut(s) 256, 604
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 4 cut(s) 141, 187, 469, 604
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 4 cut(s) 21, 140, 434, 475
BsmAI GTCTC 2 cut(s) 51, 410
BsmFI GGGAC 1 cut(s) 517
BsnI GGCC 5 cut(s) 326, 427, 562, 607, 635
Bsp1286I GDGCHC 1 cut(s) 211
Bsp143I GATC 3 cut(s) 104, 219, 586
BspACI CCGC 4 cut(s) 131, 212, 297, 628
BspANI GGCC 5 cut(s) 326, 427, 562, 607, 635
BspCNI CTCAG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 4 cut(s) 13, 505, 506, 636
BspPI GGATC 2 cut(s) 112, 581
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 2 cut(s) 140, 603
BsrI ACTGG 3 cut(s) 371, 424, 439
BssAI RCCGGY 2 cut(s) 140, 603
BssECI CCNNGG 2 cut(s) 270, 623
BssMI GATC 3 cut(s) 104, 219, 586
BssT1I CCWWGG 2 cut(s) 270, 623
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 648
BstAPI GCANNNNNTGC 1 cut(s) 248
BstBAI YACGTR 1 cut(s) 682
BstC8I GCNNGC 1 cut(s) 425
BstDEI CTNAG 1 cut(s) 414
BstENI CCTNNNNNAGG 1 cut(s) 19
BstF5I GGATG 1 cut(s) 492
BstKTI GATC 3 cut(s) 107, 222, 589
BstMAI GTCTC 2 cut(s) 51, 410
BstMBI GATC 3 cut(s) 104, 219, 586
BstMCI CGRYCG 2 cut(s) 256, 604
BstMWI GCNNNNNNNGC 3 cut(s) 206, 248, 429
BstNI CCWGG 1 cut(s) 9
BstSCI CCNGG 1 cut(s) 7
BstSNI TACGTA 1 cut(s) 682
BstV1I GCAGC 2 cut(s) 164, 235
BsuRI GGCC 5 cut(s) 326, 427, 562, 607, 635
BtsCI GGATG 1 cut(s) 492
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 425
CciI TCATGA 1 cut(s) 310
Cfr10I RCCGGY 2 cut(s) 140, 603
Cfr13I GGNCC 4 cut(s) 11, 504, 560, 634
Csp6I GTAC 3 cut(s) 388, 550, 665
CspAI ACCGGT 1 cut(s) 140
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviAII CATG 3 cut(s) 172, 311, 566
CviQI GTAC 3 cut(s) 388, 550, 665
DdeI CTNAG 1 cut(s) 414
DpnI GATC 3 cut(s) 106, 221, 588
DpnII GATC 3 cut(s) 104, 219, 586
EciI GGCGGA 1 cut(s) 120
Eco105I TACGTA 1 cut(s) 682
Eco130I CCWWGG 2 cut(s) 270, 623
Eco47I GGWCC 2 cut(s) 11, 504
EcoNI CCTNNNNNAGG 1 cut(s) 19
EcoO109I RGGNCCY 3 cut(s) 11, 504, 634
EcoRII CCWGG 1 cut(s) 7
EcoT14I CCWWGG 2 cut(s) 270, 623
EcoT22I ATGCAT 2 cut(s) 244, 619
ErhI CCWWGG 2 cut(s) 270, 623
FaeI CATG 3 cut(s) 175, 314, 569
FaqI GGGAC 1 cut(s) 517
FatI CATG 3 cut(s) 171, 310, 565
FauI CCCGC 1 cut(s) 621
FauNDI CATATG 1 cut(s) 244
FbaI TGATCA 1 cut(s) 219
Fnu4HI GCNGC 2 cut(s) 153, 249
FokI GGATG 1 cut(s) 499
Fsp4HI GCNGC 2 cut(s) 153, 249
FspBI CTAG 1 cut(s) 21
GluI GCNGC 2 cut(s) 153, 249
HaeIII GGCC 5 cut(s) 326, 427, 562, 607, 635
HapII CCGG 4 cut(s) 141, 187, 469, 604
Hin1II CATG 3 cut(s) 175, 314, 569
HinfI GANTC 3 cut(s) 4, 356, 569
HpaII CCGG 4 cut(s) 141, 187, 469, 604
HphI GGTGA 3 cut(s) 100, 155, 490
Hpy188I TCNGA 1 cut(s) 415
Hpy188III TCNNGA 3 cut(s) 311, 406, 495
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 1 cut(s) 648
HpyCH4IV ACGT 3 cut(s) 180, 264, 681
HpyCH4V TGCA 7 cut(s) 152, 242, 293, 455, 617, 673, 691
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 248, 429
HpyF3I CTNAG 1 cut(s) 414
HpySE526I ACGT 3 cut(s) 180, 264, 681
Hsp92II CATG 3 cut(s) 175, 314, 569
KflI GGGWCCC 1 cut(s) 504
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 3 cut(s) 104, 219, 586
LmnI GCTCC 2 cut(s) 206, 533
Lsp1109I GCAGC 2 cut(s) 164, 235
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 21
MaeII ACGT 3 cut(s) 180, 264, 681
MaeIII GTNAC 1 cut(s) 88
MalI GATC 3 cut(s) 106, 221, 588
MboI GATC 3 cut(s) 104, 219, 586
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 2 cut(s) 69, 320
MnlI CCTC 4 cut(s) 19, 173, 438, 625
Mph1103I ATGCAT 2 cut(s) 244, 619
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 3 cut(s) 32, 66, 288
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 4 cut(s) 141, 187, 469, 604
MspR9I CCNGG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 3 cut(s) 206, 248, 429
NdeI CATATG 1 cut(s) 244
NdeII GATC 3 cut(s) 104, 219, 586
NlaIII CATG 3 cut(s) 175, 314, 569
NlaIV GGNNCC 4 cut(s) 13, 505, 506, 636
NmuCI GTSAC 1 cut(s) 88
NsiI ATGCAT 2 cut(s) 244, 619
PagI TCATGA 1 cut(s) 310
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 3 cut(s) 4, 356, 569
PinAI ACCGGT 1 cut(s) 140
PkrI GCNGC 2 cut(s) 154, 250
Ppu21I YACGTR 1 cut(s) 682
PpuMI RGGWCCY 2 cut(s) 11, 504
PsiI TTATAA 1 cut(s) 657
Psp5II RGGWCCY 2 cut(s) 11, 504
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 4 cut(s) 13, 505, 506, 636
PspPI GGNCC 4 cut(s) 11, 504, 560, 634
PspPPI RGGWCCY 2 cut(s) 11, 504
RsaI GTAC 3 cut(s) 389, 551, 666
RsaNI GTAC 3 cut(s) 388, 550, 665
SaqAI TTAA 3 cut(s) 32, 66, 288
SatI GCNGC 2 cut(s) 153, 249
Sau3AI GATC 3 cut(s) 104, 219, 586
Sau96I GGNCC 4 cut(s) 11, 504, 560, 634
ScaI AGTACT 1 cut(s) 666
ScrFI CCNGG 1 cut(s) 9
SduI GDGCHC 1 cut(s) 211
SetI ASST 9 cut(s) 13, 30, 63, 165, 183, 267, 339, 520, 684
SfaNI GCATC 1 cut(s) 334
SinI GGWCC 2 cut(s) 11, 504
SnaBI TACGTA 1 cut(s) 682
Sse9I AATT 2 cut(s) 69, 320
SsiI CCGC 4 cut(s) 131, 212, 297, 628
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 7
StyI CCWWGG 2 cut(s) 270, 623
TaaI ACNGT 1 cut(s) 648
TaiI ACGT 3 cut(s) 183, 267, 684
TaqI TCGA 2 cut(s) 342, 585
TaqII GACCGA 1 cut(s) 270
TasI AATT 2 cut(s) 69, 320
TatI WGTACW 1 cut(s) 664
TfiI GAWTC 3 cut(s) 4, 356, 569
Tru1I TTAA 3 cut(s) 32, 66, 288
Tru9I TTAA 3 cut(s) 32, 66, 288
TscAI CASTG 1 cut(s) 439
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 2 cut(s) 152, 248
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 4 cut(s) 188, 299, 348, 503
TspGWI ACGGA 2 cut(s) 219, 499
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 2 cut(s) 11, 504
XagI CCTNNNNNAGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 1 cut(s) 21
ZrmI AGTACT 1 cut(s) 666
Zsp2I ATGCAT 2 cut(s) 244, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.