Rh4DG398500

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018599
Physical Location & Seq
Reverse (-)
61467848 .. 61468806
959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG398500.1

Sequence Viewer

Length: 882 bp
ATGCCATTTCAAGTCGACACTTATGACATAGACAATGTGAGGGTATTTCTAGATCGCACCGGTGCCGAATTCATTGGAAGTAAGTGGGAAGGCCGGCCTGACATGACTATTGTTGGCATCACTGTGTTGGCATACGGAACGAGGATGTGCCCAACTTGGCGGCAAGTGCCGATGAATTGGACTAGTGGTCCCTTTGCTAGGGAGGTTTTAACCATAATCAAGTCTCTACAAGACAAGCTCGTTAAAATTGGGAAAGCAGATATGGTGACAACATCTGTGGGATCATCAGTTTTGGGGGCTTCTTATCCGCCTTCTACCGGTGAGTTTGCAGCAAATAACCTCAATGACATGAAAGACGTTCTCCGGCTTCTATCAGCATACGGAGCACCGCTGATGATCAATGTCTATCCATACTATGCATATGCTGCCGACCGAAGCCACGTTTCCTTGGACTACGCTCTCTTTAATGCAACCGCCCCTGTGGTTCATGATGGGGAATTGGCCTACCAGAACCTTTTCGATGCTATGGTGGATTCATTCTACTGGGCAATGGAGAAAGCAGAGTACCGAAATGTGAGTATCGTGATGTCTGAGACTGGCTGGCCTCGCACTGGCACTATCCCAGAAGTTGCAGAAACATATAGCCGGAACTTGGTGAAACGGATGAAGTCAGGAGTGGGGACCCCCAAAAGACCTAACGATACTTTTGGAGCGTTTATATATGCGTACCACGATGGGCCACATGATTCTTTTGGAGTTTTCGATCCAAACCACACCGACCGGCCTTTTTATGCATTTCCAAGGCGGGAGGCCCCTGTATTACCGTTGTGTTATAATAAAGTACTTTTGCAATCTTACGTAAGATTGCAAAAAGATAAATAA

Protein Analysis

293

Amino Acids

33.0

Weight (kDa)

6.53

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 88 - 258 4e-39 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018599)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0442261
rosa_laevigata RLG00000006021
rosa_multiflora Rmu_sc0000124.1_g000058
rosa_roxburghii Rroxscaffold_5G00383320
rosa_rugosa Rorug04G0340100
rosa_samantha Rh4AG391700 Rh4AG393200 Rh4CG420400 Rh4DG398500 Rh4DG400200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
AccB1I GGYRCC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 15
AciI CCGC 5 cut(s) 160, 308, 389, 474, 805
AclWI GGATC 2 cut(s) 289, 758
AcsI RAATTY 1 cut(s) 68
AfaI GTAC 3 cut(s) 566, 728, 843
AfiI CCNNNNNNNGG 4 cut(s) 198, 317, 611, 652
AgeI ACCGGT 2 cut(s) 59, 317
AgsI TTSAA 1 cut(s) 11
AhdI GACNNNNNGTC 1 cut(s) 186
AhlI ACTAGT 1 cut(s) 182
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 228, 587
AlwI GGATC 2 cut(s) 289, 758
AoxI GGCC 7 cut(s) 91, 95, 501, 602, 737, 782, 810
ApeKI GCWGC 2 cut(s) 329, 425
ApoI RAATTY 1 cut(s) 68
AsiGI ACCGGT 2 cut(s) 59, 317
Asp700I GAANNNNTTC 1 cut(s) 515
AspS9I GGNCC 4 cut(s) 188, 681, 737, 811
AsuHPI GGTGA 3 cut(s) 277, 332, 667
AvaII GGWCC 2 cut(s) 188, 681
BaeGI GKGCMC 1 cut(s) 152
BanI GGYRCC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 341, 412
BccI CCATC 2 cut(s) 485, 728
BclI TGATCA 1 cut(s) 396
BcoDI GTCTC 2 cut(s) 228, 587
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 3 cut(s) 50, 183, 198
BisI GCNGC 3 cut(s) 161, 330, 426
BlsI GCNGC 3 cut(s) 162, 331, 427
BmcAI AGTACT 1 cut(s) 843
Bme18I GGWCC 2 cut(s) 188, 681
BmeRI GACNNNNNGTC 1 cut(s) 186
BmgT120I GGNCC 4 cut(s) 188, 681, 737, 811
BmiI GGNNCC 5 cut(s) 64, 190, 682, 683, 813
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 2 cut(s) 126, 511
BmuI ACTGGG 1 cut(s) 553
BsaAI YACGTR 1 cut(s) 859
BsaJI CCNNGG 2 cut(s) 447, 800
BsaWI WCCGGW 2 cut(s) 59, 317
Bsc4I CCNNNNNNNGG 4 cut(s) 198, 317, 611, 652
Bse118I RCCGGY 4 cut(s) 59, 93, 317, 780
Bse1I ACTGG 3 cut(s) 548, 601, 616
Bse3DI GCAATG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 447, 800
BseGI GGATG 2 cut(s) 150, 669
BseLI CCNNNNNNNGG 4 cut(s) 198, 317, 611, 652
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 3 cut(s) 548, 601, 616
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 2 cut(s) 341, 412
Bsh1285I CGRYCG 2 cut(s) 433, 781
BshFI GGCC 7 cut(s) 93, 97, 503, 604, 739, 784, 812
BshNI GGYRCC 1 cut(s) 62
BshTI ACCGGT 2 cut(s) 59, 317
BsiEI CGRYCG 2 cut(s) 433, 781
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 6 cut(s) 60, 94, 318, 364, 646, 781
BslFI GGGAC 2 cut(s) 174, 694
BslI CCNNNNNNNGG 4 cut(s) 198, 317, 611, 652
BsmAI GTCTC 2 cut(s) 228, 587
BsmFI GGGAC 2 cut(s) 174, 694
BsnI GGCC 7 cut(s) 93, 97, 503, 604, 739, 784, 812
Bsp1286I GDGCHC 2 cut(s) 152, 388
Bsp143I GATC 4 cut(s) 52, 281, 396, 763
BspACI CCGC 5 cut(s) 160, 308, 389, 474, 805
BspANI GGCC 7 cut(s) 93, 97, 503, 604, 739, 784, 812
BspCNI CTCAG 1 cut(s) 583
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 5 cut(s) 64, 190, 682, 683, 813
BspPI GGATC 2 cut(s) 289, 758
BspT107I GGYRCC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 555
BsrFI RCCGGY 4 cut(s) 59, 93, 317, 780
BsrI ACTGG 3 cut(s) 548, 601, 616
BssAI RCCGGY 4 cut(s) 59, 93, 317, 780
BssECI CCNNGG 2 cut(s) 447, 800
BssMI GATC 4 cut(s) 52, 281, 396, 763
BssT1I CCWWGG 2 cut(s) 447, 800
Bst4CI ACNGT 2 cut(s) 124, 825
BstAPI GCANNNNNTGC 1 cut(s) 425
BstBAI YACGTR 1 cut(s) 859
BstC8I GCNNGC 2 cut(s) 95, 602
BstDEI CTNAG 1 cut(s) 591
BstENI CCTNNNNNAGG 1 cut(s) 196
BstF5I GGATG 2 cut(s) 150, 669
BstKTI GATC 4 cut(s) 55, 284, 399, 766
BstMAI GTCTC 2 cut(s) 228, 587
BstMBI GATC 4 cut(s) 52, 281, 396, 763
BstMCI CGRYCG 2 cut(s) 433, 781
BstMWI GCNNNNNNNGC 4 cut(s) 166, 383, 425, 606
BstSLI GKGCMC 1 cut(s) 152
BstSNI TACGTA 1 cut(s) 859
BstV1I GCAGC 2 cut(s) 341, 412
BsuRI GGCC 7 cut(s) 93, 97, 503, 604, 739, 784, 812
BtsCI GGATG 2 cut(s) 150, 669
BtsIMutI CAGTG 2 cut(s) 120, 609
Cac8I GCNNGC 2 cut(s) 95, 602
CciI TCATGA 1 cut(s) 487
Cfr10I RCCGGY 4 cut(s) 59, 93, 317, 780
Cfr13I GGNCC 4 cut(s) 188, 681, 737, 811
Csp6I GTAC 3 cut(s) 565, 727, 842
CspAI ACCGGT 2 cut(s) 59, 317
CspCI CAANNNNNGTGG 2 cut(s) 258, 293
CviAII CATG 4 cut(s) 103, 349, 488, 743
CviQI GTAC 3 cut(s) 565, 727, 842
DdeI CTNAG 1 cut(s) 591
DpnI GATC 4 cut(s) 54, 283, 398, 765
DpnII GATC 4 cut(s) 52, 281, 396, 763
DriI GACNNNNNGTC 1 cut(s) 186
Eam1105I GACNNNNNGTC 1 cut(s) 186
EciI GGCGGA 1 cut(s) 297
Eco105I TACGTA 1 cut(s) 859
Eco130I CCWWGG 2 cut(s) 447, 800
Eco47I GGWCC 2 cut(s) 188, 681
EcoNI CCTNNNNNAGG 1 cut(s) 196
EcoO109I RGGNCCY 2 cut(s) 681, 811
EcoRI GAATTC 1 cut(s) 68
EcoT14I CCWWGG 2 cut(s) 447, 800
EcoT22I ATGCAT 2 cut(s) 421, 796
ErhI CCWWGG 2 cut(s) 447, 800
FaeI CATG 4 cut(s) 106, 352, 491, 746
FaqI GGGAC 2 cut(s) 174, 694
FatI CATG 4 cut(s) 102, 348, 487, 742
FauI CCCGC 1 cut(s) 798
FauNDI CATATG 1 cut(s) 421
FbaI TGATCA 1 cut(s) 396
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 3 cut(s) 161, 330, 426
FokI GGATG 2 cut(s) 157, 676
FseI GGCCGGCC 1 cut(s) 97
Fsp4HI GCNGC 3 cut(s) 161, 330, 426
FspBI CTAG 3 cut(s) 50, 183, 198
GluI GCNGC 3 cut(s) 161, 330, 426
HaeIII GGCC 7 cut(s) 93, 97, 503, 604, 739, 784, 812
HapII CCGG 6 cut(s) 60, 94, 318, 364, 646, 781
Hin1II CATG 4 cut(s) 106, 352, 491, 746
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 2 cut(s) 533, 746
HpaII CCGG 6 cut(s) 60, 94, 318, 364, 646, 781
HphI GGTGA 3 cut(s) 277, 332, 667
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 1 cut(s) 592
Hpy188III TCNNGA 4 cut(s) 50, 488, 583, 672
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 2 cut(s) 83, 321
HpyCH4III ACNGT 2 cut(s) 124, 825
HpyCH4IV ACGT 3 cut(s) 357, 441, 858
HpyCH4V TGCA 7 cut(s) 329, 419, 470, 632, 794, 850, 868
HpyF10VI GCNNNNNNNGC 4 cut(s) 166, 383, 425, 606
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 3 cut(s) 357, 441, 858
Hsp92II CATG 4 cut(s) 106, 352, 491, 746
KflI GGGWCCC 1 cut(s) 681
KroI GCCGGC 1 cut(s) 93
KroNI GCCGGC 1 cut(s) 95
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 4 cut(s) 52, 281, 396, 763
LmnI GCTCC 2 cut(s) 383, 710
Lsp1109I GCAGC 2 cut(s) 341, 412
LweI GCATC 2 cut(s) 126, 511
MaeI CTAG 3 cut(s) 50, 183, 198
MaeII ACGT 3 cut(s) 357, 441, 858
MaeIII GTNAC 1 cut(s) 265
MalI GATC 4 cut(s) 54, 283, 398, 765
MboI GATC 4 cut(s) 52, 281, 396, 763
MhlI GDGCHC 2 cut(s) 152, 388
MluCI AATT 4 cut(s) 68, 175, 246, 497
MnlI CCTC 6 cut(s) 33, 135, 196, 350, 615, 802
Mph1103I ATGCAT 2 cut(s) 421, 796
MroNI GCCGGC 1 cut(s) 93
MroXI GAANNNNTTC 1 cut(s) 515
MseI TTAA 3 cut(s) 209, 243, 465
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 6 cut(s) 60, 94, 318, 364, 646, 781
MwoI GCNNNNNNNGC 4 cut(s) 166, 383, 425, 606
NaeI GCCGGC 1 cut(s) 95
NdeI CATATG 1 cut(s) 421
NdeII GATC 4 cut(s) 52, 281, 396, 763
NgoMIV GCCGGC 1 cut(s) 93
NlaIII CATG 4 cut(s) 106, 352, 491, 746
NlaIV GGNNCC 5 cut(s) 64, 190, 682, 683, 813
NmuCI GTSAC 1 cut(s) 265
NsiI ATGCAT 2 cut(s) 421, 796
PagI TCATGA 1 cut(s) 487
PdiI GCCGGC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 515
PfeI GAWTC 2 cut(s) 533, 746
PinAI ACCGGT 2 cut(s) 59, 317
PkrI GCNGC 3 cut(s) 162, 331, 427
Ppu21I YACGTR 1 cut(s) 859
PpuMI RGGWCCY 1 cut(s) 681
PsiI TTATAA 1 cut(s) 834
Psp5II RGGWCCY 1 cut(s) 681
PspN4I GGNNCC 5 cut(s) 64, 190, 682, 683, 813
PspPI GGNCC 4 cut(s) 188, 681, 737, 811
PspPPI RGGWCCY 1 cut(s) 681
RigI GGCCGGCC 1 cut(s) 97
RsaI GTAC 3 cut(s) 566, 728, 843
RsaNI GTAC 3 cut(s) 565, 727, 842
RseI CAYNNNNRTG 1 cut(s) 122
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 3 cut(s) 209, 243, 465
SatI GCNGC 3 cut(s) 161, 330, 426
Sau3AI GATC 4 cut(s) 52, 281, 396, 763
Sau96I GGNCC 4 cut(s) 188, 681, 737, 811
ScaI AGTACT 1 cut(s) 843
SduI GDGCHC 2 cut(s) 152, 388
SetI ASST 8 cut(s) 207, 240, 342, 360, 444, 516, 697, 861
SfaNI GCATC 2 cut(s) 126, 511
SgrAI CRCCGGYG 1 cut(s) 59
SinI GGWCC 2 cut(s) 188, 681
SmiMI CAYNNNNRTG 1 cut(s) 122
SnaBI TACGTA 1 cut(s) 859
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 4 cut(s) 68, 175, 246, 497
SsiI CCGC 5 cut(s) 160, 308, 389, 474, 805
SspMI CTAG 3 cut(s) 50, 183, 198
StyI CCWWGG 2 cut(s) 447, 800
TaaI ACNGT 2 cut(s) 124, 825
TaiI ACGT 3 cut(s) 360, 444, 861
TaqI TCGA 3 cut(s) 15, 519, 762
TaqII GACCGA 1 cut(s) 447
TasI AATT 4 cut(s) 68, 175, 246, 497
TatI WGTACW 1 cut(s) 841
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 2 cut(s) 533, 746
Tru1I TTAA 3 cut(s) 209, 243, 465
Tru9I TTAA 3 cut(s) 209, 243, 465
TscAI CASTG 2 cut(s) 127, 616
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 329, 425
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 6 cut(s) 61, 188, 365, 476, 525, 680
TspGWI ACGGA 3 cut(s) 150, 396, 676
TspRI CASTG 2 cut(s) 127, 616
VpaK11BI GGWCC 2 cut(s) 188, 681
XagI CCTNNNNNAGG 1 cut(s) 196
XapI RAATTY 1 cut(s) 68
XbaI TCTAGA 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 15
XmnI GAANNNNTTC 1 cut(s) 515
XspI CTAG 3 cut(s) 50, 183, 198
ZrmI AGTACT 1 cut(s) 843
Zsp2I ATGCAT 2 cut(s) 421, 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.