RchiOBHm_Chr5g0042131

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0019019
Physical Location & Seq
Reverse (-)
36952814 .. 36961486
8673 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32054

Sequence Viewer

Length: 603 bp
ATGTCTTCTCTGAATCTTCTGGCTCTATCTACAAACAATTTGAATGGTAGTGTTCCAGACAATATGTGCCAACATCTTCTGAACCATCAAGGTTTGTATTTGTCTAAAAGCAATTTTGACGGCCAACTTCCATCCAAATGGTCGCGGTGCAAACATCTTCTTGTATTATCGATGGGGTATAACAATTTCAGCGGGAGCACACCCAAAGATATTTGGAACTTAACTCAGATAAAGACGATTTACCTTGGCTCCAACTATTTGACAGGTACCATACCAAATGAGATTGGTCATCTTCCATGTTTAGAGGAATTTGTCCTGGGTGGTAATAATCTCAGTGGCCTCATCCCAACCTCAATCTTCAATATGTCCACAATATCAACAATATCACTTGACAGCAACCAGTTCTCAGACTCCAGGGAGAAATTCCAACAGGCGGGCCTTTCAAAAACTTCTCTGGTGAATCATTTCTTTCTAACCATTTGTGGTGCATCCCTATTTCAAGTTTCAGCATGTAGAAGGAAAACTGGTATATCTATCTTCAAGTATGTTATTCCGGGGATCTTGTCAGCACTGCTTCTAGTGACCTCCATATTGATCTTATAG

Protein Analysis

200

Amino Acids

21.92

Weight (kDa)

8.85

Isoelectric Point (pI)

25.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 45 - 132 5.3e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 266
AccB1I GGYRCC 1 cut(s) 266
AccII CGCG 1 cut(s) 145
AciI CCGC 3 cut(s) 145, 192, 434
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 2 cut(s) 308, 422
AfaI GTAC 1 cut(s) 268
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 5 cut(s) 43, 361, 444, 500, 541
AjnI CCWGG 2 cut(s) 315, 413
Alw21I GWGCWC 1 cut(s) 200
AlwI GGATC 1 cut(s) 566
AoxI GGCC 3 cut(s) 121, 337, 436
ApoI RAATTY 2 cut(s) 308, 422
Asp700I GAANNNNTTC 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 436
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 1 cut(s) 469
BanI GGYRCC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 200
BccI CCATC 3 cut(s) 93, 139, 166
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 2 cut(s) 317, 415
BcnI CCSGG 1 cut(s) 555
BfaI CTAG 1 cut(s) 578
Bme1390I CCNGG 3 cut(s) 317, 415, 555
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 2 cut(s) 250, 268
BmrFI CCNGG 3 cut(s) 317, 415, 555
BmsI GCATC 1 cut(s) 497
BpmI CTGGAG 1 cut(s) 397
BpuMI CCSGG 1 cut(s) 555
Bsa29I ATCGAT 1 cut(s) 170
BsaJI CCNNGG 4 cut(s) 244, 316, 414, 554
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 2 cut(s) 400, 529
BseBI CCWGG 2 cut(s) 317, 415
BseCI ATCGAT 1 cut(s) 170
BseDI CCNNGG 4 cut(s) 244, 316, 414, 554
BseGI GGATG 3 cut(s) 131, 342, 488
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMII CTCAG 3 cut(s) 239, 346, 420
BseNI ACTGG 2 cut(s) 400, 529
Bsh1236I CGCG 1 cut(s) 145
BshFI GGCC 3 cut(s) 123, 339, 438
BshNI GGYRCC 1 cut(s) 266
BshVI ATCGAT 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 200
BsiSI CCGG 1 cut(s) 554
BslI CCNNNNNNNGG 1 cut(s) 433
BsnI GGCC 3 cut(s) 123, 339, 438
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 2 cut(s) 558, 594
BspACI CCGC 3 cut(s) 145, 192, 434
BspANI GGCC 3 cut(s) 123, 339, 438
BspCNI CTCAG 3 cut(s) 238, 345, 419
BspDI ATCGAT 1 cut(s) 170
BspFNI CGCG 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 250, 268
BspPI GGATC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 266
BsrI ACTGG 2 cut(s) 400, 529
BssECI CCNNGG 4 cut(s) 244, 316, 414, 554
BssMI GATC 2 cut(s) 558, 594
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 2 cut(s) 317, 415
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 3 cut(s) 225, 332, 406
BstF5I GGATG 3 cut(s) 131, 342, 488
BstFNI CGCG 1 cut(s) 145
BstKTI GATC 2 cut(s) 561, 597
BstMBI GATC 2 cut(s) 558, 594
BstNI CCWGG 2 cut(s) 317, 415
BstNSI RCATGY 1 cut(s) 513
BstSCI CCNGG 3 cut(s) 315, 413, 553
BstUI CGCG 1 cut(s) 145
BstX2I RGATCY 1 cut(s) 558
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 1 cut(s) 558
Bsu15I ATCGAT 1 cut(s) 170
BsuRI GGCC 3 cut(s) 123, 339, 438
BsuTUI ATCGAT 1 cut(s) 170
BtsCI GGATG 3 cut(s) 131, 342, 488
BtsI GCAGTG 1 cut(s) 569
BtsIMutI CAGTG 2 cut(s) 340, 569
Cac8I GCNNGC 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 436
ClaI ATCGAT 1 cut(s) 170
Csp6I GTAC 1 cut(s) 267
CviAII CATG 2 cut(s) 297, 510
CviJI RGCY 5 cut(s) 23, 123, 249, 339, 438
CviKI_1 RGCY 5 cut(s) 23, 123, 249, 339, 438
CviQI GTAC 1 cut(s) 267
DdeI CTNAG 3 cut(s) 225, 332, 406
DpnI GATC 2 cut(s) 560, 596
DpnII GATC 2 cut(s) 558, 594
EaeI YGGCCR 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 315, 413
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 2 cut(s) 300, 513
FatI CATG 2 cut(s) 296, 509
FauI CCCGC 2 cut(s) 185, 427
FokI GGATG 3 cut(s) 118, 329, 475
FspBI CTAG 1 cut(s) 578
GsuI CTGGAG 1 cut(s) 397
HaeIII GGCC 3 cut(s) 123, 339, 438
HapII CCGG 1 cut(s) 554
Hin1II CATG 2 cut(s) 300, 513
HinfI GANTC 3 cut(s) 13, 410, 460
HpaII CCGG 1 cut(s) 554
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 4 cut(s) 12, 81, 228, 409
Hpy188III TCNNGA 1 cut(s) 56
Hpy8I GTNNAC 1 cut(s) 369
HpyAV CCTTC 1 cut(s) 510
HpyCH4V TGCA 2 cut(s) 150, 488
HpyF3I CTNAG 3 cut(s) 225, 332, 406
Hsp92II CATG 2 cut(s) 300, 513
KpnI GGTACC 1 cut(s) 270
Kzo9I GATC 2 cut(s) 558, 594
LmnI GCTCC 2 cut(s) 195, 254
LweI GCATC 1 cut(s) 497
MaeI CTAG 1 cut(s) 578
MaeIII GTNAC 1 cut(s) 580
MalI GATC 2 cut(s) 560, 596
MboI GATC 2 cut(s) 558, 594
MboII GAAGA 6 cut(s) 8, 68, 149, 284, 349, 529
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 5 cut(s) 37, 112, 184, 308, 422
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 2 cut(s) 276, 451
MnlI CCTC 4 cut(s) 298, 350, 361, 595
MroXI GAANNNNTTC 1 cut(s) 464
MseI TTAA 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 136
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 1 cut(s) 554
MspR9I CCNGG 3 cut(s) 317, 415, 555
MvaI CCWGG 2 cut(s) 317, 415
MvnI CGCG 1 cut(s) 145
NciI CCSGG 1 cut(s) 555
NdeII GATC 2 cut(s) 558, 594
NlaIII CATG 2 cut(s) 300, 513
NlaIV GGNNCC 2 cut(s) 250, 268
NmuCI GTSAC 1 cut(s) 580
NspI RCATGY 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 13, 460
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Psp6I CCWGG 2 cut(s) 315, 413
PspGI CCWGG 2 cut(s) 315, 413
PspN4I GGNNCC 2 cut(s) 250, 268
PspPI GGNCC 1 cut(s) 436
PsuI RGATCY 1 cut(s) 558
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
RseI CAYNNNNRTG 1 cut(s) 136
SaqAI TTAA 1 cut(s) 221
Sau3AI GATC 2 cut(s) 558, 594
Sau96I GGNCC 1 cut(s) 436
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 3 cut(s) 317, 415, 555
SduI GDGCHC 1 cut(s) 200
SetI ASST 5 cut(s) 94, 246, 268, 353, 587
SfaNI GCATC 1 cut(s) 497
SmiMI CAYNNNNRTG 1 cut(s) 136
Sse9I AATT 5 cut(s) 37, 112, 184, 308, 422
SsiI CCGC 3 cut(s) 145, 192, 434
SspMI CTAG 1 cut(s) 578
StyD4I CCNGG 3 cut(s) 315, 413, 553
StyI CCWWGG 1 cut(s) 244
TaqI TCGA 1 cut(s) 170
TasI AATT 5 cut(s) 37, 112, 184, 308, 422
TfiI GAWTC 2 cut(s) 13, 460
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TscAI CASTG 2 cut(s) 340, 576
TseFI GTSAC 1 cut(s) 580
Tsp45I GTSAC 1 cut(s) 580
TspRI CASTG 2 cut(s) 340, 576
XapI RAATTY 2 cut(s) 308, 422
XceI RCATGY 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.