RchiOBHm_Chr5g0049831

Serrate RNA effector

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010205
Physical Location & Seq
Reverse (-)
47806878 .. 47814976
8099 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32746

Sequence Viewer

Length: 2271 bp
ATGGCCGAGATCATGAACATGCCCGTCGACTCCTTGGACCGCCGTCGCGACCGCAAAGACAAGTCTTCCGAAGAGCCTCCTCAATCCTCGCCTCCGCCGCAACAACAACCACCACCACAGCAACAGCCGCCGCCGCCTCCTCCTCCCTCGCGAAGACGCGACCGCGACGAAAAGGAGAGAGGAGATAGGGACCTCGACCGGCAGCCTCGCCGTCCGGACTACTACGACCGAGGAAATCGCTCCCCTCCGCCTCCGAGGGAGAGAGATAGGGACAGGGATTACAAGCGGCGGGGAAGCCTTAGTCCACCGCCTCCGTATAGGGACCGCAGGCACTCGCCGCCTCGGAGGTCGCCGCCGCCGCCGCCCTTTAAGCGGACGAGGAGGGAAGATGGAGGGTACGACGGCCGGAGAGGGAGTCCCAGAGGTGCCTTTGGACCTGGAGGTGATAGGAGGTCTGGATATGATTATGCAAGTGGATATGAGCGGGAGGTGGGGGCTAGACCTGCTTATGGTGATGACAGGCCTCATGGTCGTTATGCTGGACGCTCGTCTGGTGGCTATGGCCCTTCTGACTGGGATTCAGGACGTGTTGGTTTTGGTGATGCTTCCAGCACTGGGACCACTCAAAGAGAAGGGCTGATGTCATACAAACAATTCATCCAAGAGTTGGAAGATGATATTTTGCCAGCTGAGGCTGAGCGCAGATATCAAGAATACAAGGCAGAGTATATTTCTACTCAGAAGCGAGCTTATTTTAATACTCATAAAGAAGAGGAATGGCTAAAAGACAAATATCATCCCACAAACCTACTCACTGTAATTGAAAGGAGAAATGAAATAGCACGGAAAACAGCAAAGGATTTCCTGCTTGAACTGCAAAGTGGATCCTTGGACTTAGGCCCAGGTGTCAATCCCTCAGCTCCAAATAAATCTGGACATACTAGTGAACCAAATTCTGATGATGAAGCTGATGCGGATGGCAAGAGAAGACGGCATGGCAGGGAACCAAATAAAGAAAATGATCTTATTTCTACTGCTCCTAAGGCTCACCCAGTCAGTTCTGATCCTAGACGTATACAAGTTGACATTGAACAAGCCCAAGCCCTTGTCCGCAAACTTGACTCGGAAAAAGGAATTGTGGAGAACATTTTATGTGGACCTGATAATGACAAAATGAACAGGGAAAAATCTCATGGTGGTTCCACTGGCCCTGTTGTTATCGTACGGGGTTTGACATCTGTCAAGGGCCTTGAGGGTGTTGAGCTACTGGACACCCTTATTACTTATCTTTGGCGCATACATGGTGTAGATTATTATGGGATGATTGAGTCAAACGACGCTAAAGGTCTCAGGCATGTGAGAGTAGAGGTAAAGGGTTCCGATATAGGTAGTAATGGAGCTGAGTGGGAAAAGAAATTAGACTTGCATTGGCAAGAGAGATTGCATAGTCAAGATCCCTTGGAAATAATGACTGCCAAGGAAAAGATAGAAGCTGCTGCTGTTCAAGGCCTAGATCCCTTTGTGCGGAAGATTAGGGATGAAAAGTATGGCTGGAAGTATGGTTGTGGTGCCAAGGGATGCACTAAGCTCTTTCATGCTGCTGAATTTGTACACAAACATCTCAAGTTAAAACACCCTGAGCTACTGGTGGATGCGACTTTGAAAATGCGCGAGGATTTATATTTCCAGAACTACATGAATGATGCAGAGGCGCCAGGTGGAACTCCTGTTATGCAGCAATCGATACTGAGGGACAAGCCACAGAGACGTAGGCCGGGTATGGAGAATAGATTGAAAGATGAGCGTGGTAATCGGAGAGAACGCGAAAACCGAGCTAATGGTGGTGAGAGGTATGATAGGTCTGAGAATATGCAAGGTGAATTCCAGTCCAACAATGATGGTCAAGATGGAGGAAATCATGATGAACCAATGTATGATAATTTTGGTGGACAAGGGATGCATGCTGGTCCTCCTTTCCCATCAGATATACCCCCACCACCTGTATTGATGCCTGTGCCTGGTGCTGGTCCTCTCGGGCCTTTTGTTCCTGCTCCACCTGAAGTTGCAATGCAGATGTTGCGAGAGCAAGGTGGTCCTCCACCCTTTGAAGGCAGTGGTAGAAATATGTGGCCTGGACCCCAAATGAGTGGGCCAGCCCCAATTCTTGCTTTGTCTCCAGCTTTTCGACAGGATCCTCGACGTTTAAGAAGTTATCAAGATCTAGATGCACCGGAGGATGAAGTCACAGTGATAGACTACAGGAGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000381 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0005846 GO:0006139 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009507 GO:0009536 GO:0009889 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010445 GO:0010467 GO:0010468 GO:0010556 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031053 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034518 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048024 GO:0048367 GO:0048509 GO:0048519 GO:0048731 GO:0048856 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1903311 GO:1903506 GO:2000011 GO:2000026 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARS2 DUF3546

Protein Analysis

756

Amino Acids

85.41

Weight (kDa)

7.33

Isoelectric Point (pI)

72.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SERRATE_Ars2_N PF12066 217 - 298 3.3e-21 SERRATE/Ars2, N-terminal domain
ARS2 PF04959 454 - 661 3.6e-41 Arsenite-resistance protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 511
AccB1I GGYRCC 3 cut(s) 425, 1568, 1711
AccB7I CCANNNNNTGG 1 cut(s) 667
AccBSI CCGCTC 1 cut(s) 484
AccI GTMKAC 2 cut(s) 27, 1075
AccII CGCG 6 cut(s) 48, 151, 159, 165, 1671, 1824
AccIII TCCGGA 1 cut(s) 214
AclWI GGATC 7 cut(s) 879, 892, 1058, 1448, 1508, 2186, 2199
AcoI YGGCCR 2 cut(s) 3, 403
AcsI RAATTY 3 cut(s) 952, 1604, 1880
AcuI CTGAAG 1 cut(s) 2079
AcyI GRCGYC 1 cut(s) 1712
AfaI GTAC 3 cut(s) 398, 1224, 1611
AfiI CCNNNNNNNGG 8 cut(s) 372, 509, 615, 667, 1524, 2018, 2024, 2108
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 7 cut(s) 824, 872, 1091, 1505, 1663, 1795, 2108
AhlI ACTAGT 1 cut(s) 941
AjiI CACGTC 1 cut(s) 587
AjnI CCWGG 5 cut(s) 436, 901, 1714, 2017, 2131
Alw26I GTCTC 3 cut(s) 1352, 1759, 2178
AlwI GGATC 7 cut(s) 879, 892, 1058, 1448, 1508, 2186, 2199
Ama87I CYCGRG 1 cut(s) 2033
Aor13HI TCCGGA 1 cut(s) 214
ApeKI GCWGC 5 cut(s) 202, 1493, 1496, 1598, 1735
ApoI RAATTY 3 cut(s) 952, 1604, 1880
AspLEI GCGC 4 cut(s) 702, 1296, 1671, 1714
AsuC2I CCSGG 1 cut(s) 1776
AsuHPI GGTGA 6 cut(s) 455, 524, 611, 1040, 1856, 1889
AvaI CYCGRG 1 cut(s) 2033
AxyI CCTNAGG 1 cut(s) 1041
BamHI GGATCC 2 cut(s) 884, 2191
BanI GGYRCC 3 cut(s) 425, 1568, 1711
BbsI GAAGAC 3 cut(s) 57, 160, 994
BbvCI CCTCAGC 2 cut(s) 690, 916
BbvI GCAGC 5 cut(s) 214, 1480, 1483, 1585, 1747
BccI CCATC 5 cut(s) 383, 971, 1892, 1901, 1987
BceAI ACGGC 4 cut(s) 27, 195, 418, 1007
BciT130I CCWGG 5 cut(s) 438, 903, 1716, 2019, 2133
BcnI CCSGG 1 cut(s) 1776
BcoDI GTCTC 3 cut(s) 1352, 1759, 2178
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 5 cut(s) 498, 942, 1068, 1511, 2222
BfmI CTRYAG 1 cut(s) 2257
BfoI RGCGCY 1 cut(s) 1715
BfuAI ACCTGC 1 cut(s) 511
BglII AGATCT 1 cut(s) 2218
BlpI GCTNAGC 1 cut(s) 696
Bme1390I CCNGG 6 cut(s) 438, 903, 1716, 1776, 2019, 2133
BmeT110I CYCGRG 1 cut(s) 2033
BmgBI CACGTC 1 cut(s) 587
BmrFI CCNGG 6 cut(s) 438, 903, 1716, 1776, 2019, 2133
BmrI ACTGGG 3 cut(s) 583, 624, 1046
BmsI GCATC 8 cut(s) 592, 961, 1568, 1642, 1693, 1947, 1998, 2215
BmuI ACTGGG 3 cut(s) 583, 624, 1046
BoxI GACNNNNGTC 3 cut(s) 42, 547, 1238
BpiI GAAGAC 3 cut(s) 57, 160, 994
BpmI CTGGAG 2 cut(s) 459, 2160
Bpu10I CCTNAGC 3 cut(s) 690, 916, 1638
Bpu1102I GCTNAGC 1 cut(s) 696
BpuEI CTTGAG 2 cut(s) 1271, 1607
BpuMI CCSGG 1 cut(s) 1776
Bsa29I ATCGAT 1 cut(s) 1742
BsaHI GRCGYC 1 cut(s) 1712
BsaI GGTCTC 1 cut(s) 1352
BsaJI CCNNGG 9 cut(s) 33, 229, 254, 341, 888, 901, 1458, 1476, 1572
BsaWI WCCGGW 2 cut(s) 214, 2230
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bsc4I CCNNNNNNNGG 8 cut(s) 372, 509, 615, 667, 1524, 2018, 2024, 2108
Bse118I RCCGGY 1 cut(s) 198
Bse1I ACTGG 7 cut(s) 578, 619, 1052, 1210, 1272, 1650, 1885
Bse21I CCTNAGG 1 cut(s) 1041
Bse3DI GCAATG 1 cut(s) 2073
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 5 cut(s) 438, 903, 1716, 2019, 2133
BseCI ATCGAT 1 cut(s) 1742
BseDI CCNNGG 9 cut(s) 33, 229, 254, 341, 888, 901, 1458, 1476, 1572
BseGI GGATG 9 cut(s) 657, 796, 982, 1326, 1543, 1583, 1657, 1962, 2242
BseLI CCNNNNNNNGG 8 cut(s) 372, 509, 615, 667, 1524, 2018, 2024, 2108
BseMI GCAATG 1 cut(s) 2073
BseMII CTCAG 9 cut(s) 681, 687, 752, 930, 1363, 1392, 1629, 1739, 1854
BseNI ACTGG 7 cut(s) 578, 619, 1052, 1210, 1272, 1650, 1885
BseRI GAGGAG 5 cut(s) 69, 129, 132, 195, 394
BseX3I CGGCCG 1 cut(s) 403
BseXI GCAGC 5 cut(s) 214, 1480, 1483, 1585, 1747
Bsh1236I CGCG 6 cut(s) 48, 151, 159, 165, 1671, 1824
Bsh1285I CGRYCG 5 cut(s) 52, 163, 199, 229, 406
BshNI GGYRCC 3 cut(s) 425, 1568, 1711
BshVI ATCGAT 1 cut(s) 1742
BsiEI CGRYCG 5 cut(s) 52, 163, 199, 229, 406
BsiHKCI CYCGRG 1 cut(s) 2033
BsiSI CCGG 5 cut(s) 199, 215, 406, 1775, 2231
BsiWI CGTACG 1 cut(s) 1222
BslFI GGGAC 6 cut(s) 203, 284, 335, 402, 631, 1766
BslI CCNNNNNNNGG 8 cut(s) 372, 509, 615, 667, 1524, 2018, 2024, 2108
BsmAI GTCTC 3 cut(s) 1352, 1759, 2178
BsmBI CGTCTC 1 cut(s) 1759
BsmFI GGGAC 6 cut(s) 203, 284, 335, 402, 631, 1766
Bso31I GGTCTC 1 cut(s) 1352
BsoBI CYCGRG 1 cut(s) 2033
Bsp13I TCCGGA 1 cut(s) 214
Bsp1407I TGTACA 1 cut(s) 1609
Bsp143I GATC 8 cut(s) 9, 884, 1021, 1063, 1453, 1513, 2191, 2218
Bsp1720I GCTNAGC 1 cut(s) 696
Bsp68I TCGCGA 2 cut(s) 48, 151
BspCNI CTCAG 9 cut(s) 682, 688, 751, 929, 1362, 1393, 1630, 1740, 1855
BspDI ATCGAT 1 cut(s) 1742
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 6 cut(s) 48, 151, 159, 165, 1671, 1824
BspHI TCATGA 2 cut(s) 12, 1918
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 7 cut(s) 879, 892, 1058, 1448, 1508, 2186, 2199
BspQI GCTCTTC 1 cut(s) 66
BspT107I GGYRCC 3 cut(s) 425, 1568, 1711
BspTNI GGTCTC 1 cut(s) 1352
BsrBI CCGCTC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 2073
BsrFI RCCGGY 1 cut(s) 198
BsrGI TGTACA 1 cut(s) 1609
BsrI ACTGG 7 cut(s) 578, 619, 1052, 1210, 1272, 1650, 1885
BssAI RCCGGY 1 cut(s) 198
BssECI CCNNGG 9 cut(s) 33, 229, 254, 341, 888, 901, 1458, 1476, 1572
BssMI GATC 8 cut(s) 9, 884, 1021, 1063, 1453, 1513, 2191, 2218
BssNAI GTATAC 1 cut(s) 1076
BssNI GRCGYC 1 cut(s) 1712
BssT1I CCWWGG 5 cut(s) 33, 888, 1458, 1476, 1572
Bst1107I GTATAC 1 cut(s) 1076
Bst2UI CCWGG 5 cut(s) 438, 903, 1716, 2019, 2133
Bst4CI ACNGT 2 cut(s) 817, 2248
Bst6I CTCTTC 2 cut(s) 66, 765
BstACI GRCGYC 1 cut(s) 1712
BstAPI GCANNNNNTGC 1 cut(s) 2077
BstAUI TGTACA 1 cut(s) 1609
BstC8I GCNNGC 5 cut(s) 329, 687, 747, 1962, 2154
BstF5I GGATG 9 cut(s) 657, 796, 982, 1326, 1543, 1583, 1657, 1962, 2242
BstFNI CGCG 6 cut(s) 48, 151, 159, 165, 1671, 1824
BstH2I RGCGCY 1 cut(s) 1715
BstHHI GCGC 4 cut(s) 702, 1296, 1671, 1714
BstKTI GATC 8 cut(s) 12, 887, 1024, 1066, 1456, 1516, 2194, 2221
BstMAI GTCTC 3 cut(s) 1352, 1759, 2178
BstMBI GATC 8 cut(s) 9, 884, 1021, 1063, 1453, 1513, 2191, 2218
BstMCI CGRYCG 5 cut(s) 52, 163, 199, 229, 406
BstNI CCWGG 5 cut(s) 438, 903, 1716, 2019, 2133
BstNSI RCATGY 3 cut(s) 22, 1358, 1964
BstPAI GACNNNNGTC 3 cut(s) 42, 547, 1238
BstSCI CCNGG 6 cut(s) 436, 901, 1714, 1774, 2017, 2131
BstSFI CTRYAG 1 cut(s) 2257
BstUI CGCG 6 cut(s) 48, 151, 159, 165, 1671, 1824
BstV1I GCAGC 5 cut(s) 214, 1480, 1483, 1585, 1747
BstV2I GAAGAC 3 cut(s) 57, 160, 994
BstX2I RGATCY 5 cut(s) 884, 1453, 1513, 2191, 2218
BstXI CCANNNNNNTGG 1 cut(s) 2147
BstYI RGATCY 5 cut(s) 884, 1453, 1513, 2191, 2218
BstZ17I GTATAC 1 cut(s) 1076
BstZI CGGCCG 1 cut(s) 403
Bsu15I ATCGAT 1 cut(s) 1742
Bsu36I CCTNAGG 1 cut(s) 1041
BsuTUI ATCGAT 1 cut(s) 1742
BtrI CACGTC 1 cut(s) 587
BtsCI GGATG 9 cut(s) 657, 796, 982, 1326, 1543, 1583, 1657, 1962, 2242
BtsI GCAGTG 1 cut(s) 2119
BtsIMutI CAGTG 5 cut(s) 612, 813, 1203, 2119, 2253
BtuMI TCGCGA 2 cut(s) 48, 151
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 5 cut(s) 329, 687, 747, 1962, 2154
CciI TCATGA 2 cut(s) 12, 1918
CfoI GCGC 4 cut(s) 702, 1296, 1671, 1714
Cfr10I RCCGGY 1 cut(s) 198
ClaI ATCGAT 1 cut(s) 1742
CseI GACGC 3 cut(s) 165, 552, 1346
Csp6I GTAC 3 cut(s) 397, 1223, 1610
CspCI CAANNNNNGTGG 2 cut(s) 1986, 2021
CviQI GTAC 3 cut(s) 397, 1223, 1610
DinI GGCGCC 1 cut(s) 1713
DpnI GATC 8 cut(s) 11, 886, 1023, 1065, 1455, 1515, 2193, 2220
DpnII GATC 8 cut(s) 9, 884, 1021, 1063, 1453, 1513, 2191, 2218
EaeI YGGCCR 2 cut(s) 3, 403
EagI CGGCCG 1 cut(s) 403
Eam1104I CTCTTC 2 cut(s) 66, 765
EarI CTCTTC 2 cut(s) 66, 765
EciI GGCGGA 2 cut(s) 84, 237
EclXI CGGCCG 1 cut(s) 403
Eco130I CCWWGG 5 cut(s) 33, 888, 1458, 1476, 1572
Eco147I AGGCCT 2 cut(s) 523, 1509
Eco31I GGTCTC 1 cut(s) 1352
Eco32I GATATC 1 cut(s) 707
Eco52I CGGCCG 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 2079
Eco81I CCTNAGG 1 cut(s) 1041
Eco88I CYCGRG 1 cut(s) 2033
EcoO109I RGGNCCY 2 cut(s) 190, 1246
EcoRI GAATTC 1 cut(s) 1880
EcoRII CCWGG 5 cut(s) 436, 901, 1714, 2017, 2131
EcoRV GATATC 1 cut(s) 707
EcoT14I CCWWGG 5 cut(s) 33, 888, 1458, 1476, 1572
EcoT22I ATGCAT 1 cut(s) 1962
EgeI GGCGCC 1 cut(s) 1713
EheI GGCGCC 1 cut(s) 1713
ErhI CCWWGG 5 cut(s) 33, 888, 1458, 1476, 1572
Esp3I CGTCTC 1 cut(s) 1759
FaqI GGGAC 6 cut(s) 203, 284, 335, 402, 631, 1766
FauI CCCGC 2 cut(s) 282, 477
FblI GTMKAC 2 cut(s) 27, 1075
FokI GGATG 9 cut(s) 644, 783, 989, 1333, 1550, 1590, 1664, 1969, 2249
FspBI CTAG 5 cut(s) 498, 942, 1068, 1511, 2222
GlaI GCGC 4 cut(s) 701, 1295, 1670, 1713
GsuI CTGGAG 2 cut(s) 459, 2160
HaeII RGCGCY 1 cut(s) 1715
HapII CCGG 5 cut(s) 199, 215, 406, 1775, 2231
HgaI GACGC 3 cut(s) 165, 552, 1346
HhaI GCGC 4 cut(s) 702, 1296, 1671, 1714
Hin1I GRCGYC 1 cut(s) 1712
Hin6I GCGC 4 cut(s) 700, 1294, 1669, 1712
HinP1I GCGC 4 cut(s) 700, 1294, 1669, 1712
HincII GTYRAC 2 cut(s) 28, 1084
HindII GTYRAC 2 cut(s) 28, 1084
HinfI GANTC 5 cut(s) 29, 415, 578, 1121, 1328
HpaII CCGG 5 cut(s) 199, 215, 406, 1775, 2231
HphI GGTGA 6 cut(s) 455, 524, 611, 1040, 1856, 1889
Hpy166II GTNNAC 8 cut(s) 28, 305, 947, 1076, 1084, 1157, 1612, 1949
Hpy8I GTNNAC 8 cut(s) 28, 305, 947, 1076, 1084, 1157, 1612, 1949
Hpy99I CGWCG 6 cut(s) 29, 48, 170, 404, 1340, 2202
HpyAV CCTTC 3 cut(s) 576, 626, 2102
HpyCH4III ACNGT 2 cut(s) 817, 2248
HpyCH4IV ACGT 4 cut(s) 586, 1072, 1768, 2200
HpySE526I ACGT 4 cut(s) 586, 1072, 1768, 2200
Hsp92I GRCGYC 1 cut(s) 1712
HspAI GCGC 4 cut(s) 700, 1294, 1669, 1712
KasI GGCGCC 1 cut(s) 1711
Kpn2I TCCGGA 1 cut(s) 214
Kzo9I GATC 8 cut(s) 9, 884, 1021, 1063, 1453, 1513, 2191, 2218
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 6 cut(s) 245, 925, 1042, 1397, 2056, 2262
Lsp1109I GCAGC 5 cut(s) 214, 1480, 1483, 1585, 1747
LweI GCATC 8 cut(s) 592, 961, 1568, 1642, 1693, 1947, 1998, 2215
MaeI CTAG 5 cut(s) 498, 942, 1068, 1511, 2222
MaeII ACGT 4 cut(s) 586, 1072, 1768, 2200
MaeIII GTNAC 1 cut(s) 2242
MalI GATC 8 cut(s) 11, 886, 1023, 1065, 1455, 1515, 2193, 2220
MbiI CCGCTC 1 cut(s) 484
MboI GATC 8 cut(s) 9, 884, 1021, 1063, 1453, 1513, 2191, 2218
MboII GAAGA 8 cut(s) 57, 83, 165, 398, 683, 782, 999, 1540
MflI RGATCY 5 cut(s) 884, 1453, 1513, 2191, 2218
MluCI AATT 9 cut(s) 653, 819, 952, 1134, 1415, 1604, 1880, 1939, 2160
Mly113I GGCGCC 1 cut(s) 1712
MlyI GAGTC 4 cut(s) 23, 424, 1115, 1337
MmeI TCCRAC 2 cut(s) 648, 1914
Mph1103I ATGCAT 1 cut(s) 1962
MroI TCCGGA 1 cut(s) 214
MseI TTAA 4 cut(s) 369, 756, 1628, 2204
MslI CAYNNNNRTG 2 cut(s) 17, 942
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 5 cut(s) 199, 215, 406, 1775, 2231
MspR9I CCNGG 6 cut(s) 438, 903, 1716, 1776, 2019, 2133
MvaI CCWGG 5 cut(s) 438, 903, 1716, 2019, 2133
MvnI CGCG 6 cut(s) 48, 151, 159, 165, 1671, 1824
NarI GGCGCC 1 cut(s) 1712
NciI CCSGG 1 cut(s) 1776
NdeII GATC 8 cut(s) 9, 884, 1021, 1063, 1453, 1513, 2191, 2218
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 2242
NruI TCGCGA 2 cut(s) 48, 151
NsiI ATGCAT 1 cut(s) 1962
NspI RCATGY 3 cut(s) 22, 1358, 1964
PaeI GCATGC 1 cut(s) 1964
PagI TCATGA 2 cut(s) 12, 1918
PceI AGGCCT 2 cut(s) 523, 1509
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 1828
PfeI GAWTC 1 cut(s) 578
Pfl23II CGTACG 1 cut(s) 1222
PflMI CCANNNNNTGG 1 cut(s) 667
PleI GAGTC 4 cut(s) 23, 423, 1115, 1336
PluTI GGCGCC 1 cut(s) 1715
PpsI GAGTC 4 cut(s) 23, 423, 1115, 1336
PpuMI RGGWCCY 1 cut(s) 190
PshAI GACNNNNGTC 3 cut(s) 42, 547, 1238
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 5 cut(s) 436, 901, 1714, 2017, 2131
PspGI CCWGG 5 cut(s) 436, 901, 1714, 2017, 2131
PspLI CGTACG 1 cut(s) 1222
PspPPI RGGWCCY 1 cut(s) 190
PsuI RGATCY 5 cut(s) 884, 1453, 1513, 2191, 2218
PvuII CAGCTG 1 cut(s) 689
RruI TCGCGA 2 cut(s) 48, 151
RsaI GTAC 3 cut(s) 398, 1224, 1611
RsaNI GTAC 3 cut(s) 397, 1223, 1610
RseI CAYNNNNRTG 2 cut(s) 17, 942
SalI GTCGAC 1 cut(s) 26
SapI GCTCTTC 1 cut(s) 66
SaqAI TTAA 4 cut(s) 369, 756, 1628, 2204
Sau3AI GATC 8 cut(s) 9, 884, 1021, 1063, 1453, 1513, 2191, 2218
SchI GAGTC 4 cut(s) 23, 424, 1115, 1337
ScrFI CCNGG 6 cut(s) 438, 903, 1716, 1776, 2019, 2133
SfaNI GCATC 8 cut(s) 592, 961, 1568, 1642, 1693, 1947, 1998, 2215
SfcI CTRYAG 1 cut(s) 2257
SfoI GGCGCC 1 cut(s) 1713
SmiMI CAYNNNNRTG 2 cut(s) 17, 942
SmlI CTYRAG 2 cut(s) 1250, 1622
SmoI CTYRAG 2 cut(s) 1250, 1622
SpeI ACTAGT 1 cut(s) 941
SphI GCATGC 1 cut(s) 1964
Sse9I AATT 9 cut(s) 653, 819, 952, 1134, 1415, 1604, 1880, 1939, 2160
SseBI AGGCCT 2 cut(s) 523, 1509
SspDI GGCGCC 1 cut(s) 1711
SspMI CTAG 5 cut(s) 498, 942, 1068, 1511, 2222
StuI AGGCCT 2 cut(s) 523, 1509
StyD4I CCNGG 6 cut(s) 436, 901, 1714, 1774, 2017, 2131
StyI CCWWGG 5 cut(s) 33, 888, 1458, 1476, 1572
TaaI ACNGT 2 cut(s) 817, 2248
TaiI ACGT 4 cut(s) 589, 1075, 1771, 2203
TaqI TCGA 5 cut(s) 27, 195, 1742, 2185, 2197
TaqII GACCGA 1 cut(s) 243
TasI AATT 9 cut(s) 653, 819, 952, 1134, 1415, 1604, 1880, 1939, 2160
TatI WGTACW 1 cut(s) 1609
TfiI GAWTC 1 cut(s) 578
Tru1I TTAA 4 cut(s) 369, 756, 1628, 2204
Tru9I TTAA 4 cut(s) 369, 756, 1628, 2204
TscAI CASTG 5 cut(s) 619, 820, 1210, 2119, 2253
TseFI GTSAC 1 cut(s) 2242
TseI GCWGC 5 cut(s) 202, 1493, 1496, 1598, 1735
Tsp45I GTSAC 1 cut(s) 2242
TspGWI ACGGA 2 cut(s) 303, 859
TspRI CASTG 5 cut(s) 619, 820, 1210, 2119, 2253
Van91I CCANNNNNTGG 1 cut(s) 667
XapI RAATTY 3 cut(s) 952, 1604, 1880
XbaI TCTAGA 1 cut(s) 2221
XceI RCATGY 3 cut(s) 22, 1358, 1964
XmiI GTMKAC 2 cut(s) 27, 1075
XspI CTAG 5 cut(s) 498, 942, 1068, 1511, 2222
Zsp2I ATGCAT 1 cut(s) 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.