RchiOBHm_Chr6g0257331

LAG1 longevity assurance homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010380
Physical Location & Seq
Reverse (-)
12615414 .. 12622388
6975 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23082

Sequence Viewer

Length: 870 bp
ATGGAATCGTTCTGGAGCCACAATGCCGCCCCAGACTCGTTGCATTTCCTCGTCGCCGTCTACTTCGCCGTTGGATTCTTCTTCGCTAGGCTCTTCCTTGATAGAGTCATCTTCACCAGGCTTGCCATCTGGTTGTTGGGTAAAGGTTCTGCTCCATTGAAAAAGATTGATATGGTCCAACGGGCAAAGATTGTAAAATGCTCAGAGTCAATGTGGAAGTTAACATACTATACAGCCGTTGAAGCTTGCATTCTCAGTATAAGCTACCACGAGCCATGGTTCACAGATACAGCAGAGTACTTCAAAGGCTGGCCAAACCAAGAGTTGGGGCTTCCCCTAAAGCTTGTCTATATGTGTCAATGTGGATTCTACACCTACAGCATTGTTGCTCTCCTTACATGGGAGACTCGAAGGAAGGATTTCTCAGTGATGATGTCTCATCATGTAATTACCGTCTTCCTGATTGGATGCTCATACGTTACAAGCTTTTTCCGGATTGGCTCAATCATTCTTGCCCTGCATGATGGGAGTGATGTATTCATGGAAGCTGCCAAGGTCTTCAAATATTCCGAGAAGGAGCTTGCAGCAAGTGTGTTCTTTGGATGTTTTGCTGTTTCATGGCTCATACTGCGGTTAATATACTTCCCCTTTTGGGTCATAAGCTCAACAAGCTATGACCTTCTTAAGCACTTGGATTTGTCAAAGTCCTCTCATATAACACTGTACTATGTGTTCAATACGTTGTTACTGACGCTGCTTGTGTTCCACATATACTGGTGGGTTATGATATACTCAATGATTAGGAGACAACTGAATAATAGAGGAAAAGTTGGAGAAGATATAAGATCAGATTCAGACGATGATGATTAG

Protein Analysis

289

Amino Acids

33.69

Weight (kDa)

7.64

Isoelectric Point (pI)

29.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 66 - 263 9.7e-49 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19260
fragaria_vesca FvH4_2g06020 FvH4_2g06020 FvH4_2g06020 FvH4_2g06020 FvH4_2g06020
malus_domestica MD00G1035400.v1.1 MD05G1081300.v1.1
prunus_persica Prupe.8G125600_v2.0.a1
pyrus_communis pycom05g07470 pycom10g07860
rosa_chinensis RchiOBHm_Chr6g0257331
rosa_laevigata RLG00000014688
rosa_multiflora Rmu_sc0000820.1_g000006
rosa_roxburghii Rroxscaffold_7G00208620
rosa_rugosa Rorug05G0573500 Rorug05G0573600
rosa_samantha Rh6AG090800 Rh6CG078600 Rh6DG074400
rosa_wichuraiana Rw6G007920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 325
AccI GTMKAC 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 492
AciI CCGC 2 cut(s) 27, 631
AcoI YGGCCR 1 cut(s) 311
AfaI GTAC 2 cut(s) 299, 725
AfiI CCNNNNNNNGG 3 cut(s) 325, 400, 652
AflII CTTAAG 1 cut(s) 683
AgsI TTSAA 5 cut(s) 160, 242, 304, 562, 736
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 8 cut(s) 245, 264, 343, 486, 548, 580, 663, 672
AluI AGCT 8 cut(s) 245, 264, 343, 486, 548, 580, 663, 672
Alw26I GTCTC 3 cut(s) 398, 441, 799
Aor13HI TCCGGA 1 cut(s) 492
AoxI GGCC 1 cut(s) 311
ApeKI GCWGC 3 cut(s) 548, 584, 754
Asp700I GAANNNNTTC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 313
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 2 cut(s) 448, 550
BbvI GCAGC 3 cut(s) 535, 596, 741
BccI CCATC 2 cut(s) 134, 518
BceAI ACGGC 3 cut(s) 41, 53, 221
BciT130I CCWGG 1 cut(s) 118
BcoDI GTCTC 3 cut(s) 398, 441, 799
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 1 cut(s) 376
BfrI CTTAAG 1 cut(s) 683
BisI GCNGC 4 cut(s) 27, 549, 585, 755
BlsI GCNGC 4 cut(s) 28, 550, 586, 756
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 17
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 1 cut(s) 458
BpiI GAAGAC 2 cut(s) 448, 550
BpmI CTGGAG 1 cut(s) 34
BsaJI CCNNGG 2 cut(s) 275, 552
BsaWI WCCGGW 1 cut(s) 492
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 400, 652
Bse1I ACTGG 1 cut(s) 779
BseAI TCCGGA 1 cut(s) 492
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 2 cut(s) 275, 552
BseGI GGATG 2 cut(s) 473, 608
BseLI CCNNNNNNNGG 3 cut(s) 325, 400, 652
BseMII CTCAG 3 cut(s) 216, 268, 438
BseNI ACTGG 1 cut(s) 779
BseXI GCAGC 3 cut(s) 535, 596, 741
BshFI GGCC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 325, 400, 652
BsmAI GTCTC 3 cut(s) 398, 441, 799
BsmI GAATGC 1 cut(s) 249
BsnI GGCC 1 cut(s) 313
Bsp13I TCCGGA 1 cut(s) 492
Bsp143I GATC 1 cut(s) 845
Bsp19I CCATGG 1 cut(s) 275
BspACI CCGC 2 cut(s) 27, 631
BspANI GGCC 1 cut(s) 313
BspCNI CTCAG 3 cut(s) 215, 267, 437
BspEI TCCGGA 1 cut(s) 492
BspLI GGNNCC 1 cut(s) 17
BspQI GCTCTTC 1 cut(s) 98
BspTI CTTAAG 1 cut(s) 683
BsrI ACTGG 1 cut(s) 779
BssECI CCNNGG 2 cut(s) 275, 552
BssMI GATC 1 cut(s) 845
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 2 cut(s) 275, 552
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 454, 723
Bst6I CTCTTC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 683
BstC8I GCNNGC 4 cut(s) 123, 247, 311, 582
BstDEI CTNAG 3 cut(s) 202, 254, 424
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 2 cut(s) 473, 608
BstKTI GATC 1 cut(s) 848
BstMAI GTCTC 3 cut(s) 398, 441, 799
BstMBI GATC 1 cut(s) 845
BstMWI GCNNNNNNNGC 3 cut(s) 242, 628, 669
BstNI CCWGG 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 376
BstV1I GCAGC 3 cut(s) 535, 596, 741
BstV2I GAAGAC 2 cut(s) 448, 550
BsuRI GGCC 1 cut(s) 313
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 2 cut(s) 473, 608
BtsIMutI CAGTG 2 cut(s) 432, 719
Cac8I GCNNGC 4 cut(s) 123, 247, 311, 582
Cfr13I GGNCC 1 cut(s) 175
CseI GACGC 1 cut(s) 760
Csp6I GTAC 2 cut(s) 298, 724
CviAII CATG 6 cut(s) 276, 399, 443, 521, 541, 618
CviQI GTAC 2 cut(s) 298, 724
DdeI CTNAG 3 cut(s) 202, 254, 424
DpnI GATC 1 cut(s) 847
DpnII GATC 1 cut(s) 845
EaeI YGGCCR 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 2 cut(s) 275, 552
Eco47I GGWCC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 116
EcoT14I CCWWGG 2 cut(s) 275, 552
ErhI CCWWGG 2 cut(s) 275, 552
FaeI CATG 6 cut(s) 279, 402, 446, 524, 544, 621
FatI CATG 6 cut(s) 275, 398, 442, 520, 540, 617
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 27, 549, 585, 755
FokI GGATG 2 cut(s) 480, 615
Fsp4HI GCNGC 4 cut(s) 27, 549, 585, 755
FspBI CTAG 1 cut(s) 87
GluI GCNGC 4 cut(s) 27, 549, 585, 755
GsuI CTGGAG 1 cut(s) 34
HaeIII GGCC 1 cut(s) 313
HapII CCGG 1 cut(s) 493
HgaI GACGC 1 cut(s) 760
Hin1II CATG 6 cut(s) 279, 402, 446, 524, 544, 621
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HindIII AAGCTT 3 cut(s) 243, 341, 484
HinfI GANTC 8 cut(s) 5, 35, 75, 105, 206, 366, 406, 851
HpaI GTTAAC 1 cut(s) 222
HpaII CCGG 1 cut(s) 493
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 3 cut(s) 61, 222, 282
Hpy188I TCNGA 4 cut(s) 205, 571, 850, 856
Hpy188III TCNNGA 3 cut(s) 13, 460, 493
Hpy8I GTNNAC 3 cut(s) 61, 222, 282
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 4 cut(s) 405, 409, 568, 689
HpyCH4III ACNGT 2 cut(s) 454, 723
HpyCH4IV ACGT 2 cut(s) 477, 740
HpyCH4V TGCA 4 cut(s) 43, 249, 520, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 628, 669
HpyF3I CTNAG 3 cut(s) 202, 254, 424
HpySE526I ACGT 2 cut(s) 477, 740
Hsp92II CATG 6 cut(s) 279, 402, 446, 524, 544, 621
Kpn2I TCCGGA 1 cut(s) 492
KspAI GTTAAC 1 cut(s) 222
Kzo9I GATC 1 cut(s) 845
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 15, 157, 577
LpnPI CCDG 9 cut(s) 45, 103, 115, 130, 295, 473, 506, 530, 760
Lsp1109I GCAGC 3 cut(s) 535, 596, 741
LweI GCATC 1 cut(s) 458
MaeI CTAG 1 cut(s) 87
MaeII ACGT 2 cut(s) 477, 740
MaeIII GTNAC 2 cut(s) 478, 744
MalI GATC 1 cut(s) 847
MboI GATC 1 cut(s) 845
MboII GAAGA 7 cut(s) 70, 73, 85, 103, 448, 550, 848
MlsI TGGCCA 1 cut(s) 313
MluCI AATT 1 cut(s) 447
MluNI TGGCCA 1 cut(s) 313
MlyI GAGTC 4 cut(s) 29, 114, 215, 400
MmeI TCCRAC 3 cut(s) 52, 202, 811
MnlI CCTC 3 cut(s) 59, 718, 815
Mox20I TGGCCA 1 cut(s) 313
MroI TCCGGA 1 cut(s) 492
MroXI GAANNNNTTC 1 cut(s) 419
MscI TGGCCA 1 cut(s) 313
MseI TTAA 3 cut(s) 221, 635, 684
Msp20I TGGCCA 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 683
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 1 cut(s) 118
Mva1269I GAATGC 1 cut(s) 249
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 3 cut(s) 242, 628, 669
NcoI CCATGG 1 cut(s) 275
NdeII GATC 1 cut(s) 845
NlaIII CATG 6 cut(s) 279, 402, 446, 524, 544, 621
NlaIV GGNNCC 1 cut(s) 17
PciSI GCTCTTC 1 cut(s) 98
PctI GAATGC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 4 cut(s) 5, 75, 366, 851
PflMI CCANNNNNTGG 1 cut(s) 325
PkrI GCNGC 4 cut(s) 28, 550, 586, 756
PleI GAGTC 4 cut(s) 29, 113, 214, 400
PpsI GAGTC 4 cut(s) 29, 113, 214, 400
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 17
PspPI GGNCC 1 cut(s) 175
PsrI GAACNNNNNNTAC 2 cut(s) 716, 748
RsaI GTAC 2 cut(s) 299, 725
RsaNI GTAC 2 cut(s) 298, 724
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 3 cut(s) 221, 635, 684
SatI GCNGC 4 cut(s) 27, 549, 585, 755
Sau3AI GATC 1 cut(s) 845
Sau96I GGNCC 1 cut(s) 175
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 4 cut(s) 29, 114, 215, 400
ScrFI CCNGG 1 cut(s) 118
SfaNI GCATC 1 cut(s) 458
SfcI CTRYAG 1 cut(s) 376
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 1 cut(s) 447
SsiI CCGC 2 cut(s) 27, 631
SspI AATATT 1 cut(s) 566
SspMI CTAG 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 2 cut(s) 275, 552
TaaI ACNGT 2 cut(s) 454, 723
TaiI ACGT 2 cut(s) 480, 743
TaqI TCGA 1 cut(s) 409
TasI AATT 1 cut(s) 447
TatI WGTACW 2 cut(s) 297, 723
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 4 cut(s) 5, 75, 366, 851
Tru1I TTAA 3 cut(s) 221, 635, 684
Tru9I TTAA 3 cut(s) 221, 635, 684
TscAI CASTG 2 cut(s) 432, 726
TseI GCWGC 3 cut(s) 548, 584, 754
TspDTI ATGAA 2 cut(s) 529, 606
TspRI CASTG 2 cut(s) 432, 726
Van91I CCANNNNNTGG 1 cut(s) 325
Vha464I CTTAAG 1 cut(s) 683
VpaK11BI GGWCC 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 87
ZrmI AGTACT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.