RchiOBHm_Chr6g0261481

Cold-regulated 413 plasma membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005630
Physical Location & Seq
Forward (+)
16351673 .. 16352344
672 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23451

Sequence Viewer

Length: 366 bp
ATGCTCACAGGGCTGTTAATCCCATATATATATATATTCTGTAGCCGTCCTTCACTAATTTTCTCTATCTTCAGGGGAGAAATTGGAAGCTGGATTGCCTTCGTTGCGGTTATCTTGCGATTCTTTTTCCCTAAACGATTCCCAGAATGGGCTGAATTGCCGGCTGCGCTGATTCTTCTGATGATTGTGGCTCCGAGTTTAATTGCAACCACTGTGAGGGATGACTGGATCGGAGTTGCAATATGTCTCGTCATTGCAGATTACTTGCTGCAAGAACACATCCGGGCATCCGGTGGATTCAGAAACGCTTTCACACAACCCCATGGCGTATCAAATACTGTTGGCATAATCTGTCTGTTCATCTAA

Protein Analysis

121

Amino Acids

13.55

Weight (kDa)

7.76

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WCOR413 PF05562 1 - 119 2.7e-54 Cold acclimation protein WCOR413
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 107
AclWI GGATC 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 148, 216
AluBI AGCT 1 cut(s) 90
AluI AGCT 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 164, 268
AspLEI GCGC 1 cut(s) 169
AsuC2I CCSGG 1 cut(s) 284
BarI GAAGNNNNNNTAC 2 cut(s) 34, 66
BbvI GCAGC 2 cut(s) 151, 255
BceAI ACGGC 1 cut(s) 30
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 40
BisI GCNGC 2 cut(s) 165, 269
BlsI GCNGC 2 cut(s) 166, 270
Bme1390I CCNGG 1 cut(s) 284
BmiI GGNNCC 1 cut(s) 192
BmrFI CCNGG 1 cut(s) 284
BmsI GCATC 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 284
BsaJI CCNNGG 1 cut(s) 322
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 216
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 230
Bse3DI GCAATG 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 3 cut(s) 226, 279, 287
BseLI CCNNNNNNNGG 2 cut(s) 148, 216
BseMI GCAATG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 230
BseXI GCAGC 2 cut(s) 151, 255
BsiSI CCGG 3 cut(s) 161, 283, 291
BslI CCNNNNNNNGG 2 cut(s) 148, 216
BsmAI GTCTC 1 cut(s) 251
Bsp143I GATC 1 cut(s) 228
Bsp19I CCATGG 1 cut(s) 322
BspACI CCGC 1 cut(s) 107
BspLI GGNNCC 1 cut(s) 192
BspPI GGATC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 252
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 1 cut(s) 230
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 214, 340
BstC8I GCNNGC 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 322
BstF5I GGATG 3 cut(s) 226, 279, 287
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 1 cut(s) 231
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 1 cut(s) 228
BstMWI GCNNNNNNNGC 3 cut(s) 10, 104, 166
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 151, 255
BtgI CCRYGG 1 cut(s) 322
BtsCI GGATG 3 cut(s) 226, 279, 287
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 162
CfoI GCGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 160
CviAII CATG 1 cut(s) 323
CviJI RGCY 6 cut(s) 13, 45, 90, 152, 164, 191
CviKI_1 RGCY 6 cut(s) 13, 45, 90, 152, 164, 191
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 1 cut(s) 326
FaiI YATR 9 cut(s) 25, 27, 29, 31, 33, 35, 244, 324, 347
FatI CATG 1 cut(s) 322
Fnu4HI GCNGC 2 cut(s) 165, 269
FokI GGATG 3 cut(s) 233, 266, 274
Fsp4HI GCNGC 2 cut(s) 165, 269
GlaI GCGC 1 cut(s) 168
GluI GCNGC 2 cut(s) 165, 269
HapII CCGG 3 cut(s) 161, 283, 291
HhaI GCGC 1 cut(s) 169
Hin1II CATG 1 cut(s) 326
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 4 cut(s) 120, 138, 172, 297
HpaII CCGG 3 cut(s) 161, 283, 291
Hpy188I TCNGA 4 cut(s) 180, 195, 233, 302
HpyAV CCTTC 2 cut(s) 60, 109
HpyCH4III ACNGT 2 cut(s) 214, 340
HpyCH4V TGCA 4 cut(s) 206, 239, 257, 271
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 104, 166
Hsp92II CATG 1 cut(s) 326
HspAI GCGC 1 cut(s) 167
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
Kzo9I GATC 1 cut(s) 228
LmnI GCTCC 1 cut(s) 196
LpnPI CCDG 7 cut(s) 58, 76, 156, 174, 211, 296, 304
Lsp1109I GCAGC 2 cut(s) 151, 255
LweI GCATC 1 cut(s) 296
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 2 cut(s) 61, 167
MluCI AATT 4 cut(s) 57, 81, 155, 201
MnlI CCTC 1 cut(s) 210
MroNI GCCGGC 1 cut(s) 160
MseI TTAA 2 cut(s) 17, 200
MspI CCGG 3 cut(s) 161, 283, 291
MspR9I CCNGG 1 cut(s) 284
MwoI GCNNNNNNNGC 3 cut(s) 10, 104, 166
NaeI GCCGGC 1 cut(s) 162
NciI CCSGG 1 cut(s) 284
NcoI CCATGG 1 cut(s) 322
NdeII GATC 1 cut(s) 228
NgoMIV GCCGGC 1 cut(s) 160
NlaIII CATG 1 cut(s) 326
NlaIV GGNNCC 1 cut(s) 192
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 4 cut(s) 120, 138, 172, 297
PkrI GCNGC 2 cut(s) 166, 270
PspN4I GGNNCC 1 cut(s) 192
SaqAI TTAA 2 cut(s) 17, 200
SatI GCNGC 2 cut(s) 165, 269
Sau3AI GATC 1 cut(s) 228
ScrFI CCNGG 1 cut(s) 284
SetI ASST 1 cut(s) 92
SfaNI GCATC 1 cut(s) 296
SfcI CTRYAG 1 cut(s) 40
Sse9I AATT 4 cut(s) 57, 81, 155, 201
SsiI CCGC 1 cut(s) 107
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 2 cut(s) 214, 340
TasI AATT 4 cut(s) 57, 81, 155, 201
TfiI GAWTC 4 cut(s) 120, 138, 172, 297
Tru1I TTAA 2 cut(s) 17, 200
Tru9I TTAA 2 cut(s) 17, 200
TscAI CASTG 1 cut(s) 217
TseI GCWGC 2 cut(s) 164, 268
TspDTI ATGAA 1 cut(s) 349
TspRI CASTG 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.