RchiOBHm_Chr6g0302501

domain in TBC and LysM domain containing proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013334
Physical Location & Seq
Reverse (-)
62590133 .. 62593298
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27172

Sequence Viewer

Length: 1614 bp
ATGGGAGCGTCATCTTCGTCAGAGCAGACGACGGTTCCGATTGAGCAACGAGAGGCCGAAACCCTAGCAGCCTCCACCGGAGCTCTTTCTACGCTTCAGAAGTGTTTCTCTAACCTCGCCGATCCTCACTCCGGTGCTATTCCCTACGACTCTCTTCAGCAATGCTTTCGGTTAAGTTACGAGAATCCGGTATGCGAAGGTCTTGCAATTCCGGTATCATTTCCGGGAGTGCTGGATCATGTTGGATGCTGCATAGCGGAGCTGTTCTTCGTGACGGAAAAAGGGGGAGTGAGTTGGATTGAGTTCGTTAGAGGTTACAATAGGTGTTGTGCAAGAAAGACTTTGTCCATGTCTGTCACTAGTTTGCTGAGAGTGTTTAGTGTGGCACTTAAGAAAGCCGGTTTGCCTTGTAATTTGGAATTTGAAACTGGTGATGATGATTGTAAGATTAGCGGGTGGCTTATGCCTGTGGATGTGCTTATGCTTCTTTGGATGTGTTGGGCCATGTCTTGGTATTCTAGGACTAGAGAATTTCCGAGAGAGAAAGGCAAGTTGTGTCTGCCGGATGTTAAGCATATTGTGTTGTCGGCTATTGAATCATGTGCCGAAGCTGAAAGTGGACTGAATGTTTGGGACTGTGAGCTTTCGGGTTTGGAAGTTCAACTTCCTGTGGGGAAGTTTCTTACTTGGGTTTTGAAGACAGTGTCTACTCTCCCTGGTTGCTTTGCCGAGTTTGTGTATGCAGTACTCAAACATTCTGCTTCTCAAGAGGCTGGACTGGAATGTTCGACTTCTTCAGCTGCAGTTACTTCGTCAACTATGGCATGCAGTTCTAATCTTTTGACCCCTGGAATAGCCTGGGCTGTTTCCCTCACACTAACAGGCCCTCTAAGTGAAGAGATCTCAAGGGCATGCTTCCCTTCAGATGCTAATGGAATAAATAATGACCTACTTTACAGGTCACATCTTCATGGGAGAGGGTTGAATAGATTCTGGTCTAATGTTCAAGGTTACCAAGGTCCACTGCTTGTTCTGATTTCTGCAACCTCAGAAGATGGTAGTGCCAATAAAAGAAATTGGATTGTAGGTGTTCTCACACATCAAGGTTTTGAGAATAGGGATCTCTTCTATGGAAGTTCAGGAAGTCTGTACGCTATAAGTCCAGTCTTTCATGTGTATCCACCTACTGGAAAGGAAAGGAACTTTGTGTATAGCCATCTCCATCCCACTGGCAAGGTGTATGAGGTGAAACCGAAGCCTGTTGGAATAGGATTTGGTGGAACTCTGGGAAATGAGAGAATTTATGTGGATGAAGATTTTTCTAAGGTTACCATTCGCCATCATGCAGCTGACAAAACTTATCAGTCTGGCTCCCTCTTTCCTGATCAGGGCTTTCTACCTGTTGAAGCTTTGATCACAGAAGTTGAAATTTGGGGACTTGGTGGAAGGGGCGCAAAGGATGTGCAGGATTCATATAAGAATAGGGAACAACTTTTCACTGAGCAAAGACGAAAGGTTGACTTGAAAACATTTGCAAACTGGGAGGATTCACCTGAAAAGATGATGATGGACATGGTCTCCAACCCCAATGCAGTTCGACGTGAAGACCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TLD

Protein Analysis

537

Amino Acids

59.19

Weight (kDa)

5.64

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TLD PF07534 303 - 481 2.4e-25 TLDc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013334)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39590
fragaria_vesca FvH4_2g38790 FvH4_2g38790
malus_domestica MD09G1232000.v1.1
prunus_persica Prupe.1G368700_v2.0.a1
pyrus_communis pycom09g15130
rosa_chinensis RchiOBHm_Chr6g0302501
rosa_laevigata RLG00000011167
rosa_multiflora Rmu_sc0011021.1_g000010 Rmu_sc0018485.1_g000004 Rmu_sc0042878.1_g000001
rosa_roxburghii Rroxscaffold_7G00165710
rosa_rugosa Rorug06G0314600
rosa_samantha Rh6AG427800 Rh6BG474900 Rh6CG439900 Rh6DG426900
rosa_wichuraiana Rw6G037000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 510, 1187
AccI GTMKAC 1 cut(s) 707
AciI CCGC 2 cut(s) 257, 453
AclWI GGATC 3 cut(s) 116, 243, 1128
AcsI RAATTY 4 cut(s) 419, 530, 1299, 1428
AcuI CTGAAG 4 cut(s) 80, 140, 780, 906
AfaI GTAC 2 cut(s) 747, 1151
AfiI CCNNNNNNNGG 4 cut(s) 131, 510, 1187, 1388
AflII CTTAAG 1 cut(s) 389
AgeI ACCGGT 1 cut(s) 1608
AgsI TTSAA 9 cut(s) 425, 596, 662, 697, 985, 1007, 1406, 1427, 1525
AhlI ACTAGT 1 cut(s) 359
AjiI CACGTC 1 cut(s) 1601
AjnI CCWGG 3 cut(s) 715, 847, 857
AleI CACNNNNGTG 1 cut(s) 132
AluBI AGCT 7 cut(s) 83, 262, 611, 643, 800, 1349, 1409
AluI AGCT 7 cut(s) 83, 262, 611, 643, 800, 1349, 1409
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 1 cut(s) 1582
AlwI GGATC 3 cut(s) 116, 243, 1128
AoxI GGCC 3 cut(s) 54, 501, 883
ApeKI GCWGC 4 cut(s) 68, 249, 800, 1346
ApoI RAATTY 4 cut(s) 419, 530, 1299, 1428
ArsI GACNNNNNNTTYG 2 cut(s) 612, 644
AsiGI ACCGGT 1 cut(s) 1608
Asp700I GAANNNNTTC 2 cut(s) 104, 989
AspLEI GCGC 1 cut(s) 1454
AspS9I GGNCC 3 cut(s) 501, 884, 1019
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 3 cut(s) 443, 1258, 1542
AvaII GGWCC 1 cut(s) 1019
BanII GRGCYC 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 704
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 4 cut(s) 80, 236, 787, 1358
BccI CCATC 5 cut(s) 1049, 1224, 1230, 1347, 1561
BcgI CGANNNNNNTGC 6 cut(s) 149, 183, 185, 219, 792, 826
BciT130I CCWGG 3 cut(s) 717, 849, 859
BciVI GTATCC 1 cut(s) 1188
BclI TGATCA 2 cut(s) 1384, 1413
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 1582
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 4 cut(s) 65, 360, 519, 525
BfmI CTRYAG 1 cut(s) 801
BfrI CTTAAG 1 cut(s) 389
BfuI GTATCC 1 cut(s) 1188
BglII AGATCT 1 cut(s) 900
BisI GCNGC 4 cut(s) 69, 250, 801, 1347
BlsI GCNGC 4 cut(s) 70, 251, 802, 1348
BmcAI AGTACT 1 cut(s) 747
Bme1390I CCNGG 4 cut(s) 225, 717, 849, 859
Bme18I GGWCC 1 cut(s) 1019
BmgBI CACGTC 1 cut(s) 1601
BmgT120I GGNCC 3 cut(s) 501, 884, 1019
BmiI GGNNCC 2 cut(s) 36, 1372
BmrFI CCNGG 4 cut(s) 225, 717, 849, 859
BmrI ACTGGG 1 cut(s) 1549
BmsI GCATC 2 cut(s) 236, 916
BmuI ACTGGG 1 cut(s) 1549
BpiI GAAGAC 1 cut(s) 704
BpuEI CTTGAG 2 cut(s) 750, 889
BpuMI CCSGG 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 1582
BsaJI CCNNGG 4 cut(s) 715, 847, 858, 1015
BsaWI WCCGGW 5 cut(s) 77, 131, 187, 211, 1608
BsaXI ACNNNNNCTCC 2 cut(s) 1562, 1592
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 510, 1187, 1388
Bse118I RCCGGY 2 cut(s) 398, 1608
Bse1I ACTGG 6 cut(s) 433, 783, 1163, 1192, 1234, 1544
Bse3DI GCAATG 1 cut(s) 167
BseBI CCWGG 3 cut(s) 717, 849, 859
BseDI CCNNGG 4 cut(s) 715, 847, 858, 1015
BseGI GGATG 7 cut(s) 251, 478, 498, 571, 1222, 1315, 1465
BseLI CCNNNNNNNGG 4 cut(s) 131, 510, 1187, 1388
BseMI GCAATG 1 cut(s) 167
BseMII CTCAG 3 cut(s) 359, 1062, 1491
BseNI ACTGG 6 cut(s) 433, 783, 1163, 1192, 1234, 1544
BseXI GCAGC 4 cut(s) 80, 236, 787, 1358
BsgI GTGCAG 1 cut(s) 1484
BshFI GGCC 3 cut(s) 56, 503, 885
BshTI ACCGGT 1 cut(s) 1608
BsiHKAI GWGCWC 1 cut(s) 85
BsiSI CCGG 8 cut(s) 78, 132, 188, 212, 224, 399, 563, 1609
BslFI GGGAC 2 cut(s) 647, 1449
BslI CCNNNNNNNGG 4 cut(s) 131, 510, 1187, 1388
BsmAI GTCTC 1 cut(s) 1582
BsmFI GGGAC 2 cut(s) 647, 1449
BsnI GGCC 3 cut(s) 56, 503, 885
Bso31I GGTCTC 1 cut(s) 1582
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 6 cut(s) 121, 235, 900, 1120, 1384, 1413
BspACI CCGC 2 cut(s) 257, 453
BspANI GGCC 3 cut(s) 56, 503, 885
BspCNI CTCAG 3 cut(s) 360, 1061, 1492
BspLI GGNNCC 2 cut(s) 36, 1372
BspMAI CTGCAG 1 cut(s) 805
BspPI GGATC 3 cut(s) 116, 243, 1128
BspTI CTTAAG 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 1582
BsrDI GCAATG 1 cut(s) 167
BsrFI RCCGGY 2 cut(s) 398, 1608
BsrI ACTGG 6 cut(s) 433, 783, 1163, 1192, 1234, 1544
BssAI RCCGGY 2 cut(s) 398, 1608
BssECI CCNNGG 4 cut(s) 715, 847, 858, 1015
BssMI GATC 6 cut(s) 121, 235, 900, 1120, 1384, 1413
BssT1I CCWWGG 1 cut(s) 1015
Bst2UI CCWGG 3 cut(s) 717, 849, 859
Bst4CI ACNGT 3 cut(s) 34, 638, 703
Bst6I CTCTTC 3 cut(s) 159, 891, 1130
BstAFI CTTAAG 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 826, 913
BstDEI CTNAG 5 cut(s) 368, 890, 1048, 1323, 1500
BstEII GGTNACC 2 cut(s) 1010, 1327
BstF5I GGATG 7 cut(s) 251, 478, 498, 571, 1222, 1315, 1465
BstHHI GCGC 1 cut(s) 1454
BstKTI GATC 6 cut(s) 124, 238, 903, 1123, 1387, 1416
BstMAI GTCTC 1 cut(s) 1582
BstMBI GATC 6 cut(s) 121, 235, 900, 1120, 1384, 1413
BstNI CCWGG 3 cut(s) 717, 849, 859
BstNSI RCATGY 2 cut(s) 828, 915
BstPI GGTNACC 2 cut(s) 1010, 1327
BstSCI CCNGG 4 cut(s) 223, 715, 847, 857
BstSFI CTRYAG 1 cut(s) 801
BstV1I GCAGC 4 cut(s) 80, 236, 787, 1358
BstV2I GAAGAC 1 cut(s) 704
BstX2I RGATCY 2 cut(s) 900, 1120
BstXI CCANNNNNNTGG 1 cut(s) 1229
BstYI RGATCY 2 cut(s) 900, 1120
BsuI GTATCC 1 cut(s) 1188
BsuRI GGCC 3 cut(s) 56, 503, 885
BtrI CACGTC 1 cut(s) 1601
BtsCI GGATG 7 cut(s) 251, 478, 498, 571, 1222, 1315, 1465
BtsI GCAGTG 1 cut(s) 1022
BtsIMutI CAGTG 4 cut(s) 708, 1022, 1227, 1497
Cac8I GCNNGC 2 cut(s) 826, 913
CfoI GCGC 1 cut(s) 1454
Cfr10I RCCGGY 2 cut(s) 398, 1608
Cfr13I GGNCC 3 cut(s) 501, 884, 1019
Csp6I GTAC 2 cut(s) 746, 1150
CspAI ACCGGT 1 cut(s) 1608
CviQI GTAC 2 cut(s) 746, 1150
DdeI CTNAG 5 cut(s) 368, 890, 1048, 1323, 1500
DpnI GATC 6 cut(s) 123, 237, 902, 1122, 1386, 1415
DpnII GATC 6 cut(s) 121, 235, 900, 1120, 1384, 1413
Eam1104I CTCTTC 3 cut(s) 159, 891, 1130
EarI CTCTTC 3 cut(s) 159, 891, 1130
Ecl136II GAGCTC 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 1015
Eco24I GRGCYC 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 1582
Eco47I GGWCC 1 cut(s) 1019
Eco53kI GAGCTC 1 cut(s) 83
Eco57I CTGAAG 4 cut(s) 80, 140, 780, 906
Eco91I GGTNACC 2 cut(s) 1010, 1327
EcoICRI GAGCTC 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 884
EcoO65I GGTNACC 2 cut(s) 1010, 1327
EcoRII CCWGG 3 cut(s) 715, 847, 857
EcoT14I CCWWGG 1 cut(s) 1015
EcoT38I GRGCYC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 1015
FalI AAGNNNNNCTT 6 cut(s) 325, 357, 648, 680, 1505, 1537
FaqI GGGAC 2 cut(s) 647, 1449
FauI CCCGC 1 cut(s) 446
FbaI TGATCA 2 cut(s) 1384, 1413
FblI GTMKAC 1 cut(s) 707
Fnu4HI GCNGC 4 cut(s) 69, 250, 801, 1347
FokI GGATG 7 cut(s) 258, 485, 505, 578, 1209, 1322, 1472
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 4 cut(s) 69, 250, 801, 1347
FspBI CTAG 4 cut(s) 65, 360, 519, 525
GlaI GCGC 1 cut(s) 1453
GluI GCNGC 4 cut(s) 69, 250, 801, 1347
HaeIII GGCC 3 cut(s) 56, 503, 885
HapII CCGG 8 cut(s) 78, 132, 188, 212, 224, 399, 563, 1609
HhaI GCGC 1 cut(s) 1454
Hin6I GCGC 1 cut(s) 1452
HinP1I GCGC 1 cut(s) 1452
HincII GTYRAC 2 cut(s) 816, 1519
HindII GTYRAC 2 cut(s) 816, 1519
HindIII AAGCTT 1 cut(s) 1407
HinfI GANTC 6 cut(s) 149, 184, 596, 990, 1469, 1547
HpaII CCGG 8 cut(s) 78, 132, 188, 212, 224, 399, 563, 1609
HphI GGTGA 3 cut(s) 443, 1258, 1542
Hpy166II GTNNAC 5 cut(s) 620, 708, 816, 1022, 1519
Hpy188I TCNGA 7 cut(s) 22, 39, 99, 537, 925, 1035, 1051
Hpy188III TCNNGA 4 cut(s) 271, 767, 1140, 1382
Hpy8I GTNNAC 5 cut(s) 620, 708, 816, 1022, 1519
Hpy99I CGWCG 2 cut(s) 34, 1602
HpyAV CCTTC 3 cut(s) 191, 930, 1440
HpyCH4III ACNGT 3 cut(s) 34, 638, 703
HpyCH4IV ACGT 1 cut(s) 1600
HpyF3I CTNAG 5 cut(s) 368, 890, 1048, 1323, 1500
HpySE526I ACGT 1 cut(s) 1600
HspAI GCGC 1 cut(s) 1452
Ksp22I TGATCA 2 cut(s) 1384, 1413
Kzo9I GATC 6 cut(s) 121, 235, 900, 1120, 1384, 1413
LmnI GCTCC 4 cut(s) 5, 80, 259, 1376
Lsp1109I GCAGC 4 cut(s) 80, 236, 787, 1358
LweI GCATC 2 cut(s) 236, 916
MaeI CTAG 4 cut(s) 65, 360, 519, 525
MaeII ACGT 1 cut(s) 1600
MaeIII GTNAC 8 cut(s) 176, 271, 314, 355, 805, 960, 1010, 1327
MalI GATC 6 cut(s) 123, 237, 902, 1122, 1386, 1415
MboI GATC 6 cut(s) 121, 235, 900, 1120, 1384, 1413
MflI RGATCY 2 cut(s) 900, 1120
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 7 cut(s) 207, 412, 419, 530, 1075, 1299, 1428
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 4 cut(s) 223, 275, 1243, 1605
MroXI GAANNNNTTC 2 cut(s) 104, 989
MseI TTAA 3 cut(s) 173, 390, 570
MslI CAYNNNNRTG 2 cut(s) 132, 969
MspA1I CMGCKG 2 cut(s) 800, 1349
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 8 cut(s) 78, 132, 188, 212, 224, 399, 563, 1609
MspR9I CCNGG 4 cut(s) 225, 717, 849, 859
MvaI CCWGG 3 cut(s) 717, 849, 859
NciI CCSGG 1 cut(s) 225
NdeII GATC 6 cut(s) 121, 235, 900, 1120, 1384, 1413
NlaIV GGNNCC 2 cut(s) 36, 1372
NmeAIII GCCGAG 1 cut(s) 754
NmuCI GTSAC 3 cut(s) 271, 355, 960
NspI RCATGY 2 cut(s) 828, 915
OliI CACNNNNGTG 1 cut(s) 132
PaeI GCATGC 2 cut(s) 828, 915
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PdmI GAANNNNTTC 2 cut(s) 104, 989
PfeI GAWTC 5 cut(s) 184, 596, 990, 1469, 1547
PflFI GACNNNGTC 3 cut(s) 343, 703, 1574
PflMI CCANNNNNTGG 2 cut(s) 510, 1187
PfoI TCCNGGA 1 cut(s) 223
PinAI ACCGGT 1 cut(s) 1608
PkrI GCNGC 4 cut(s) 70, 251, 802, 1348
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Psp124BI GAGCTC 1 cut(s) 85
Psp6I CCWGG 3 cut(s) 715, 847, 857
PspEI GGTNACC 2 cut(s) 1010, 1327
PspGI CCWGG 3 cut(s) 715, 847, 857
PspN4I GGNNCC 2 cut(s) 36, 1372
PspPI GGNCC 3 cut(s) 501, 884, 1019
PstI CTGCAG 1 cut(s) 805
PsuI RGATCY 2 cut(s) 900, 1120
PsyI GACNNNGTC 3 cut(s) 343, 703, 1574
PvuII CAGCTG 2 cut(s) 800, 1349
RsaI GTAC 2 cut(s) 747, 1151
RsaNI GTAC 2 cut(s) 746, 1150
RseI CAYNNNNRTG 2 cut(s) 132, 969
SacI GAGCTC 1 cut(s) 85
SaqAI TTAA 3 cut(s) 173, 390, 570
SatI GCNGC 4 cut(s) 69, 250, 801, 1347
Sau3AI GATC 6 cut(s) 121, 235, 900, 1120, 1384, 1413
Sau96I GGNCC 3 cut(s) 501, 884, 1019
ScaI AGTACT 1 cut(s) 747
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 4 cut(s) 225, 717, 849, 859
SduI GDGCHC 1 cut(s) 85
SfaNI GCATC 2 cut(s) 236, 916
SfcI CTRYAG 1 cut(s) 801
SinI GGWCC 1 cut(s) 1019
SmiMI CAYNNNNRTG 2 cut(s) 132, 969
SmlI CTYRAG 3 cut(s) 389, 765, 904
SmoI CTYRAG 3 cut(s) 389, 765, 904
SpeI ACTAGT 1 cut(s) 359
SphI GCATGC 2 cut(s) 828, 915
Sse9I AATT 7 cut(s) 207, 412, 419, 530, 1075, 1299, 1428
SsiI CCGC 2 cut(s) 257, 453
SspMI CTAG 4 cut(s) 65, 360, 519, 525
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 4 cut(s) 223, 715, 847, 857
StyI CCWWGG 1 cut(s) 1015
TaaI ACNGT 3 cut(s) 34, 638, 703
TaiI ACGT 1 cut(s) 1603
TaqI TCGA 2 cut(s) 788, 1597
TasI AATT 7 cut(s) 207, 412, 419, 530, 1075, 1299, 1428
TatI WGTACW 1 cut(s) 745
TfiI GAWTC 5 cut(s) 184, 596, 990, 1469, 1547
Tru1I TTAA 3 cut(s) 173, 390, 570
Tru9I TTAA 3 cut(s) 173, 390, 570
TscAI CASTG 4 cut(s) 708, 1029, 1234, 1504
TseFI GTSAC 3 cut(s) 271, 355, 960
TseI GCWGC 4 cut(s) 68, 249, 800, 1346
Tsp45I GTSAC 3 cut(s) 271, 355, 960
TspDTI ATGAA 4 cut(s) 959, 1160, 1326, 1461
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 4 cut(s) 708, 1029, 1234, 1504
Tth111I GACNNNGTC 3 cut(s) 343, 703, 1574
Van91I CCANNNNNTGG 2 cut(s) 510, 1187
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 1019
XapI RAATTY 4 cut(s) 419, 530, 1299, 1428
XceI RCATGY 2 cut(s) 828, 915
XmiI GTMKAC 1 cut(s) 707
XmnI GAANNNNTTC 2 cut(s) 104, 989
XspI CTAG 4 cut(s) 65, 360, 519, 525
ZrmI AGTACT 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.