RchiOBHm_Chr7g0216491

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0018641
Physical Location & Seq
Reverse (-)
34029978 .. 34037389
7412 bp
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UTR
Exon/CDS
Intron
PRQ19371

Sequence Viewer

Length: 303 bp
ATGGAGTCACTACTGCTACATACTGCCTACTCGATGGAGAACACTGAACTTTGGTTAACACATTTACTAGACTTCGTAGTCCAGGTTGGACTGGCTTTCTATGTCTTTATATGGATAGCTAGCACTTACCATCTACTGTTAGTTATACCAGTGTTTGTTGCTGGAGTTATCAAGTATGGAGAGAGGACTTGGGTCCTGCAGTCTACGAGCAGCAAGAAGCTTGCTGACTCCCTGCTTCTACCTTCTTATCCTAGGAATAATTGTGCTAATTTTGAGGAGAAATATAGCACTATAAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

100

Amino Acids

11.46

Weight (kDa)

6.25

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 8 - 88 1.2e-18 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AccI GTMKAC 1 cut(s) 203
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 3 cut(s) 119, 220, 299
AluI AGCT 3 cut(s) 119, 220, 299
ApeKI GCWGC 1 cut(s) 210
AspA2I CCTAGG 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 193
AsuNHI GCTAGC 1 cut(s) 119
AvaII GGWCC 1 cut(s) 193
AvrII CCTAGG 1 cut(s) 251
BbvI GCAGC 1 cut(s) 222
BccI CCATC 2 cut(s) 28, 138
BciT130I CCWGG 1 cut(s) 83
BfaI CTAG 3 cut(s) 68, 120, 252
BfmI CTRYAG 1 cut(s) 197
BisI GCNGC 1 cut(s) 211
BlnI CCTAGG 1 cut(s) 251
BlsI GCNGC 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 83
BmtI GCTAGC 1 cut(s) 123
BoxI GACNNNNGTC 1 cut(s) 191
BpmI CTGGAG 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 96, 149
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 251
BseNI ACTGG 2 cut(s) 96, 149
BseRI GAGGAG 1 cut(s) 290
BseXI GCAGC 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 194
BspMAI CTGCAG 1 cut(s) 201
BspOI GCTAGC 1 cut(s) 123
BsrI ACTGG 2 cut(s) 96, 149
BssECI CCNNGG 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 138
BstC8I GCNNGC 2 cut(s) 121, 222
BstNI CCWGG 1 cut(s) 83
BstPAI GACNNNNGTC 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 42, 156
Cac8I GCNNGC 2 cut(s) 121, 222
Cfr13I GGNCC 1 cut(s) 193
CviJI RGCY 4 cut(s) 95, 119, 220, 299
CviKI_1 RGCY 4 cut(s) 95, 119, 220, 299
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 251
ErhI CCWWGG 1 cut(s) 251
FaiI YATR 8 cut(s) 21, 102, 110, 112, 146, 177, 285, 293
FblI GTMKAC 1 cut(s) 203
Fnu4HI GCNGC 1 cut(s) 211
Fsp4HI GCNGC 1 cut(s) 211
FspBI CTAG 3 cut(s) 68, 120, 252
GluI GCNGC 1 cut(s) 211
GsuI CTGGAG 1 cut(s) 183
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HindIII AAGCTT 2 cut(s) 218, 297
HinfI GANTC 2 cut(s) 5, 227
HpaI GTTAAC 1 cut(s) 57
Hpy166II GTNNAC 2 cut(s) 57, 204
Hpy8I GTNNAC 2 cut(s) 57, 204
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4V TGCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 57
LpnPI CCDG 7 cut(s) 68, 77, 95, 147, 162, 209, 245
Lsp1109I GCAGC 1 cut(s) 222
MaeI CTAG 3 cut(s) 68, 120, 252
MaeIII GTNAC 1 cut(s) 6
MluCI AATT 2 cut(s) 259, 268
MlyI GAGTC 2 cut(s) 14, 221
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 2 cut(s) 177, 268
MseI TTAA 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 83
MvaI CCWGG 1 cut(s) 83
NheI GCTAGC 1 cut(s) 119
NlaIV GGNNCC 1 cut(s) 194
NmuCI GTSAC 1 cut(s) 6
PkrI GCNGC 1 cut(s) 212
PleI GAGTC 2 cut(s) 13, 221
PpsI GAGTC 2 cut(s) 13, 221
PpuMI RGGWCCY 1 cut(s) 193
PshAI GACNNNNGTC 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 1 cut(s) 193
PspPPI RGGWCCY 1 cut(s) 193
PstI CTGCAG 1 cut(s) 201
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 2 cut(s) 14, 221
ScrFI CCNGG 1 cut(s) 83
SetI ASST 5 cut(s) 87, 121, 222, 244, 301
SfcI CTRYAG 1 cut(s) 197
SinI GGWCC 1 cut(s) 193
Sse9I AATT 2 cut(s) 259, 268
SspMI CTAG 3 cut(s) 68, 120, 252
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 1 cut(s) 251
TaaI ACNGT 1 cut(s) 138
TaqI TCGA 1 cut(s) 32
TasI AATT 2 cut(s) 259, 268
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 2 cut(s) 49, 156
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 1 cut(s) 210
Tsp45I GTSAC 1 cut(s) 6
TspRI CASTG 2 cut(s) 49, 156
VpaK11BI GGWCC 1 cut(s) 193
XmaJI CCTAGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 203
XspI CTAG 3 cut(s) 68, 120, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.