Rh2BG250800

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018641
Physical Location & Seq
Reverse (-)
25693541 .. 25693882
342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG250800.1

Sequence Viewer

Length: 342 bp
ATGGTAATCTATTGGCATTCTGGGCTCCCTTTCTTATTTTGCACCTTGGGTGGTCCAAACACCATAATTGCAATTGCTATGGCAGACAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGTTGTAGCTGCAATGTACGTCTTCGGTATGCAGTTCTCTAACCAAAACAAGCTACTGTGGCCAACACTCTTCCTGTTTGTTCCAGGACTTGTCAAGTACGGCGAGCGAACACGTGCTTTATATCTTTCCAGCTTGGAAAGTCTCAAGGAAACCATACTTGCAGTATCGGAACCTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

113

Amino Acids

12.78

Weight (kDa)

6.03

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 10 - 110 4.5e-27 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 98, 188
AcvI CACGTG 1 cut(s) 242
AfaI GTAC 4 cut(s) 146, 227, 323, 335
AflIII ACRYGT 1 cut(s) 239
AjnI CCWGG 3 cut(s) 126, 211, 301
AluBI AGCT 3 cut(s) 137, 181, 261
AluI AGCT 3 cut(s) 137, 181, 261
Alw26I GTCTC 2 cut(s) 122, 275
AoxI GGCC 2 cut(s) 98, 188
ApeKI GCWGC 1 cut(s) 137
AspS9I GGNCC 2 cut(s) 53, 305
AvaII GGWCC 2 cut(s) 53, 305
BalI TGGCCA 2 cut(s) 100, 190
BanII GRGCYC 1 cut(s) 27
BbrPI CACGTG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 142
BbvI GCAGC 1 cut(s) 124
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 3 cut(s) 128, 213, 303
BcoDI GTCTC 2 cut(s) 122, 275
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 3 cut(s) 128, 213, 303
Bme18I GGWCC 2 cut(s) 53, 305
BmgT120I GGNCC 2 cut(s) 53, 305
BmiI GGNNCC 2 cut(s) 26, 300
BmrFI CCNGG 3 cut(s) 128, 213, 303
BpiI GAAGAC 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 257
BsaAI YACGTR 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 45, 101
BsaWI WCCGGW 1 cut(s) 307
Bse3DI GCAATG 1 cut(s) 147
BseBI CCWGG 3 cut(s) 128, 213, 303
BseDI CCNNGG 2 cut(s) 45, 101
BseGI GGATG 1 cut(s) 334
BseMI GCAATG 1 cut(s) 147
BseXI GCAGC 1 cut(s) 124
BshFI GGCC 2 cut(s) 100, 190
BsiSI CCGG 1 cut(s) 308
BsmAI GTCTC 2 cut(s) 122, 275
BsmI GAATGC 1 cut(s) 16
BsnI GGCC 2 cut(s) 100, 190
Bso31I GGTCTC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 27
BspANI GGCC 2 cut(s) 100, 190
BspLI GGNNCC 2 cut(s) 26, 300
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 147
BssECI CCNNGG 2 cut(s) 45, 101
BssT1I CCWWGG 2 cut(s) 45, 101
Bst2UI CCWGG 3 cut(s) 128, 213, 303
Bst4CI ACNGT 1 cut(s) 186
Bst6I CTCTTC 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 89
BstBAI YACGTR 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 233
BstF5I GGATG 1 cut(s) 334
BstMAI GTCTC 2 cut(s) 122, 275
BstMWI GCNNNNNNNGC 3 cut(s) 22, 89, 187
BstNI CCWGG 3 cut(s) 128, 213, 303
BstSCI CCNGG 3 cut(s) 126, 211, 301
BstV1I GCAGC 1 cut(s) 124
BstV2I GAAGAC 1 cut(s) 142
BsuRI GGCC 2 cut(s) 100, 190
BtsCI GGATG 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 53, 305
Csp6I GTAC 4 cut(s) 145, 226, 322, 334
CviAII CATG 1 cut(s) 325
CviJI RGCY 6 cut(s) 25, 100, 137, 181, 190, 261
CviKI_1 RGCY 6 cut(s) 25, 100, 137, 181, 190, 261
CviQI GTAC 4 cut(s) 145, 226, 322, 334
EaeI YGGCCR 2 cut(s) 98, 188
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Eco130I CCWWGG 2 cut(s) 45, 101
Eco24I GRGCYC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 2 cut(s) 53, 305
Eco72I CACGTG 1 cut(s) 242
EcoRII CCWGG 3 cut(s) 126, 211, 301
EcoT14I CCWWGG 2 cut(s) 45, 101
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 2 cut(s) 45, 101
FaeI CATG 1 cut(s) 328
FaiI YATR 8 cut(s) 65, 80, 122, 158, 250, 284, 315, 326
FatI CATG 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 138
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 138
HaeIII GGCC 2 cut(s) 100, 190
HapII CCGG 1 cut(s) 308
Hin1II CATG 1 cut(s) 328
HpaII CCGG 1 cut(s) 308
Hpy188I TCNGA 1 cut(s) 298
HpyCH4III ACNGT 1 cut(s) 186
HpyCH4IV ACGT 2 cut(s) 147, 241
HpyCH4V TGCA 6 cut(s) 42, 71, 140, 160, 290, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 89, 187
HpySE526I ACGT 2 cut(s) 147, 241
Hsp92II CATG 1 cut(s) 328
LmnI GCTCC 1 cut(s) 30
Lsp1109I GCAGC 1 cut(s) 124
MaeII ACGT 2 cut(s) 147, 241
MboII GAAGA 2 cut(s) 142, 190
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 2 cut(s) 100, 190
MluCI AATT 2 cut(s) 66, 72
MluNI TGGCCA 2 cut(s) 100, 190
Mox20I TGGCCA 2 cut(s) 100, 190
MscI TGGCCA 2 cut(s) 100, 190
Msp20I TGGCCA 2 cut(s) 100, 190
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 3 cut(s) 128, 213, 303
MunI CAATTG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 16
MvaI CCWGG 3 cut(s) 128, 213, 303
MwoI GCNNNNNNNGC 3 cut(s) 22, 89, 187
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 2 cut(s) 26, 300
PctI GAATGC 1 cut(s) 16
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 1 cut(s) 139
PmaCI CACGTG 1 cut(s) 242
PmlI CACGTG 1 cut(s) 242
Ppu21I YACGTR 1 cut(s) 242
Psp6I CCWGG 3 cut(s) 126, 211, 301
PspCI CACGTG 1 cut(s) 242
PspGI CCWGG 3 cut(s) 126, 211, 301
PspN4I GGNNCC 2 cut(s) 26, 300
PspPI GGNCC 2 cut(s) 53, 305
RsaI GTAC 4 cut(s) 146, 227, 323, 335
RsaNI GTAC 4 cut(s) 145, 226, 322, 334
SatI GCNGC 1 cut(s) 138
Sau96I GGNCC 2 cut(s) 53, 305
ScaI AGTACT 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 128, 213, 303
SduI GDGCHC 1 cut(s) 27
SetI ASST 8 cut(s) 47, 132, 139, 150, 183, 244, 263, 304
SinI GGWCC 2 cut(s) 53, 305
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 2 cut(s) 66, 72
StyD4I CCNGG 3 cut(s) 126, 211, 301
StyI CCWWGG 2 cut(s) 45, 101
TaaI ACNGT 1 cut(s) 186
TaiI ACGT 2 cut(s) 150, 244
TasI AATT 2 cut(s) 66, 72
TatI WGTACW 2 cut(s) 321, 333
TseI GCWGC 1 cut(s) 137
VpaK11BI GGWCC 2 cut(s) 53, 305
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.