Rh3BG354600

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0018641
Physical Location & Seq
Reverse (-)
42029485 .. 42029820
336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG354600.1

Sequence Viewer

Length: 336 bp
ATGGCTTCTTGGGCTCTAGTTCTTCTGGTGCACCTCAGTGGTCCATACACCATTACTGCCTACTTGATGGAGGACAATGAACTTTGGTTAAGGCACTTACTAGGCTTAGTAGTCCAGGATGGAGTGGCTTTTTATATCTTGATATGGACAGCTAGCACTTACCATCTATTGTTAGTTATACCAGTGTGTGTTGCTGGAATTATCAAGTATGGAAAGAGGACTTGGGTCATGTGGTCTGTGAGCAGTGAGAAGCTTGCTGACTCCTTGCTTCCACCTCCTTATCCTGGGAATGATTATGCTAATTTTGCAGAGAAATATACCGCTGTAAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

111

Amino Acids

12.47

Weight (kDa)

6.24

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 1 - 103 1.9e-29 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 321
AfiI CCNNNNNNNGG 1 cut(s) 284
AjnI CCWGG 2 cut(s) 114, 283
AjuI GAANNNNNNNTTGG 2 cut(s) 205, 237
AleI CACNNNNGTG 1 cut(s) 36
AluBI AGCT 3 cut(s) 152, 253, 332
AluI AGCT 3 cut(s) 152, 253, 332
Alw21I GWGCWC 1 cut(s) 33
Alw44I GTGCAC 1 cut(s) 29
ApaLI GTGCAC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 41
AsuNHI GCTAGC 1 cut(s) 152
AvaII GGWCC 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 16
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 3 cut(s) 61, 113, 171
BciT130I CCWGG 2 cut(s) 116, 285
BfaI CTAG 3 cut(s) 17, 101, 153
Bme1390I CCNGG 2 cut(s) 116, 285
Bme18I GGWCC 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 41
BmrFI CCNGG 2 cut(s) 116, 285
BmtI GCTAGC 1 cut(s) 156
BoxI GACNNNNGTC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 182
BseBI CCWGG 2 cut(s) 116, 285
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 49
BseNI ACTGG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 16, 33
BspACI CCGC 1 cut(s) 321
BspCNI CTCAG 1 cut(s) 48
BspOI GCTAGC 1 cut(s) 156
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 284
Bst2UI CCWGG 2 cut(s) 116, 285
BstC8I GCNNGC 2 cut(s) 154, 255
BstDEI CTNAG 2 cut(s) 35, 106
BstF5I GGATG 1 cut(s) 124
BstMWI GCNNNNNNNGC 3 cut(s) 11, 305, 329
BstNI CCWGG 2 cut(s) 116, 285
BstPAI GACNNNNGTC 1 cut(s) 224
BstSCI CCNGG 2 cut(s) 114, 283
BstSLI GKGCMC 1 cut(s) 33
BtsCI GGATG 1 cut(s) 124
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 3 cut(s) 43, 189, 250
Cac8I GCNNGC 2 cut(s) 154, 255
Cfr13I GGNCC 1 cut(s) 41
CviAII CATG 1 cut(s) 229
CviJI RGCY 7 cut(s) 5, 14, 105, 128, 152, 253, 332
CviKI_1 RGCY 7 cut(s) 5, 14, 105, 128, 152, 253, 332
DdeI CTNAG 2 cut(s) 35, 106
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 41
EcoRII CCWGG 2 cut(s) 114, 283
EcoT38I GRGCYC 1 cut(s) 16
FaeI CATG 1 cut(s) 232
FaiI YATR 8 cut(s) 46, 135, 145, 179, 210, 230, 297, 318
FatI CATG 1 cut(s) 228
FokI GGATG 1 cut(s) 131
FriOI GRGCYC 1 cut(s) 16
FspBI CTAG 3 cut(s) 17, 101, 153
Hin1II CATG 1 cut(s) 232
HindIII AAGCTT 2 cut(s) 251, 330
HinfI GANTC 1 cut(s) 260
Hpy166II GTNNAC 1 cut(s) 31
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 1 cut(s) 31
HpyCH4V TGCA 2 cut(s) 31, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 305, 329
HpyF3I CTNAG 2 cut(s) 35, 106
Hsp92II CATG 1 cut(s) 232
LpnPI CCDG 7 cut(s) 11, 101, 128, 180, 195, 270, 297
MaeI CTAG 3 cut(s) 17, 101, 153
MboII GAAGA 1 cut(s) 14
MhlI GDGCHC 2 cut(s) 16, 33
MluCI AATT 2 cut(s) 198, 301
MlyI GAGTC 1 cut(s) 254
MnlI CCTC 4 cut(s) 44, 64, 210, 285
MseI TTAA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 323
MspR9I CCNGG 2 cut(s) 116, 285
MvaI CCWGG 2 cut(s) 116, 285
MwoI GCNNNNNNNGC 3 cut(s) 11, 305, 329
NheI GCTAGC 1 cut(s) 152
NlaIII CATG 1 cut(s) 232
OliI CACNNNNGTG 1 cut(s) 36
PfoI TCCNGGA 1 cut(s) 114
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PshAI GACNNNNGTC 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 114, 283
PspGI CCWGG 2 cut(s) 114, 283
PspPI GGNCC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 1 cut(s) 89
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 116, 285
SduI GDGCHC 2 cut(s) 16, 33
SetI ASST 5 cut(s) 36, 154, 255, 277, 334
SinI GGWCC 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 36
Sse9I AATT 2 cut(s) 198, 301
SsiI CCGC 1 cut(s) 321
SspMI CTAG 3 cut(s) 17, 101, 153
StyD4I CCNGG 2 cut(s) 114, 283
TasI AATT 2 cut(s) 198, 301
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TscAI CASTG 3 cut(s) 43, 189, 250
TspDTI ATGAA 1 cut(s) 93
TspRI CASTG 3 cut(s) 43, 189, 250
VneI GTGCAC 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 41
XspI CTAG 3 cut(s) 17, 101, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.