Rh3CG351500

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0018641
Physical Location & Seq
Reverse (-)
42395018 .. 42418660
23643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG351500.1

Sequence Viewer

Length: 744 bp
ATGATCATGGCTTCTTGGGCTCTAGTTCTTCTGGTGCACCTCGGTGGTCCATATACCATTACTGCCTACTTGATGGAGGACAATGAACTTTGGTTAAGGCACTTACTAGGCTTAGTAGTCCAGGATGGAGTGGCTTTTTATATCTTGATATGGACAGCTAGCACTTACCATCTATTGTTAGTTATACTAGTGTGTGTTGCTGGAATTATCAAGTATGGAAAGAGGACTTGGGTCCTGTGGTCTGTGAGCAGTGAGAAGCTTGCTGACTCCTTGCTTCCACCTCCTTATCCTGGGAATGATTATGCTAATTTTGCAGAGAAATATATCGCTGTTCTTGAAATGGTTGTTAGATTAGTGAAGGTGAAGGATGATATGTTGCTGCTGAATCCGGAATGGCAAACCCAACATCAATTATATGGTAAATTCGGGGACCTGACTGACCACCACCACCACCACCTGACTTTGTCCTCCTCCGCCTCCAACGGCAACATTGACTGCGTCCTCTTCCAAGACCTCGTCGGGATCGTCACTCTCGTCCAGTCCCTCATCGATCCGAAAGCCAGCAGTTCATTTGCGCGGCGCGGTTCAGTCACCTACAGCAAGATTCCTTATCAAAGAAAGAAGCCAGAAGGTGTGCAACACGTTAAAAAGAAGGGCTGGGCATTGGTCGAGAGATTACCAAAAGATGGAAGCTTCAAAATTGGCCTTGTGGTTTTCTTTGTGACTCACCCCCAAAATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

247

Amino Acids

27.88

Weight (kDa)

8.72

Isoelectric Point (pI)

25.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 2 - 125 1e-30 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 686
AccII CGCG 2 cut(s) 577, 582
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 3 cut(s) 474, 577, 582
AclWI GGATC 2 cut(s) 530, 545
AcsI RAATTY 1 cut(s) 422
AfiI CCNNNNNNNGG 2 cut(s) 290, 686
AflIII ACRYGT 1 cut(s) 640
AgsI TTSAA 2 cut(s) 338, 697
AhlI ACTAGT 1 cut(s) 187
AjnI CCWGG 2 cut(s) 120, 289
AjuI GAANNNNNNNTTGG 2 cut(s) 211, 243
AleI CACNNNNGTG 1 cut(s) 42
AluBI AGCT 3 cut(s) 158, 259, 693
AluI AGCT 3 cut(s) 158, 259, 693
Alw21I GWGCWC 1 cut(s) 39
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 2 cut(s) 530, 545
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 1 cut(s) 703
ApaLI GTGCAC 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 379
ApoI RAATTY 1 cut(s) 422
AspLEI GCGC 2 cut(s) 577, 582
AspS9I GGNCC 3 cut(s) 47, 232, 430
AsuHPI GGTGA 3 cut(s) 373, 583, 719
AsuNHI GCTAGC 1 cut(s) 158
AvaII GGWCC 3 cut(s) 47, 232, 430
BaeGI GKGCMC 1 cut(s) 39
BanII GRGCYC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 366
BccI CCATC 4 cut(s) 67, 119, 177, 680
BceAI ACGGC 1 cut(s) 499
BciT130I CCWGG 2 cut(s) 122, 291
BclI TGATCA 1 cut(s) 3
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 4 cut(s) 23, 107, 159, 188
BfmI CTRYAG 1 cut(s) 595
BisI GCNGC 2 cut(s) 380, 578
BlsI GCNGC 2 cut(s) 381, 579
Bme1390I CCNGG 2 cut(s) 122, 291
Bme18I GGWCC 3 cut(s) 47, 232, 430
BmgT120I GGNCC 3 cut(s) 47, 232, 430
BmiI GGNNCC 2 cut(s) 233, 431
BmrFI CCNGG 2 cut(s) 122, 291
BmtI GCTAGC 1 cut(s) 162
BoxI GACNNNNGTC 1 cut(s) 230
Bsa29I ATCGAT 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 40, 290
BsaWI WCCGGW 1 cut(s) 388
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 686
Bse1I ACTGG 1 cut(s) 538
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 2 cut(s) 122, 291
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 2 cut(s) 40, 290
BseGI GGATG 2 cut(s) 130, 373
BseLI CCNNNNNNNGG 2 cut(s) 290, 686
BseNI ACTGG 1 cut(s) 538
BseRI GAGGAG 1 cut(s) 460
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 1 cut(s) 366
BseYI CCCAGC 1 cut(s) 657
Bsh1236I CGCG 2 cut(s) 577, 582
BshFI GGCC 1 cut(s) 705
BshVI ATCGAT 1 cut(s) 549
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 1 cut(s) 389
BslFI GGGAC 2 cut(s) 443, 526
BslI CCNNNNNNNGG 2 cut(s) 290, 686
BsmFI GGGAC 2 cut(s) 443, 526
BsnI GGCC 1 cut(s) 705
Bsp1286I GDGCHC 2 cut(s) 22, 39
Bsp13I TCCGGA 1 cut(s) 388
Bsp143I GATC 3 cut(s) 3, 522, 550
BspACI CCGC 3 cut(s) 474, 577, 582
BspANI GGCC 1 cut(s) 705
BspDI ATCGAT 1 cut(s) 549
BspEI TCCGGA 1 cut(s) 388
BspFNI CGCG 2 cut(s) 577, 582
BspLI GGNNCC 2 cut(s) 233, 431
BspOI GCTAGC 1 cut(s) 162
BspPI GGATC 2 cut(s) 530, 545
BsrI ACTGG 1 cut(s) 538
BssECI CCNNGG 2 cut(s) 40, 290
BssMI GATC 3 cut(s) 3, 522, 550
Bst2UI CCWGG 2 cut(s) 122, 291
Bst6I CTCTTC 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 160, 261, 562
BstDEI CTNAG 1 cut(s) 112
BstF5I GGATG 2 cut(s) 130, 373
BstFNI CGCG 2 cut(s) 577, 582
BstHHI GCGC 2 cut(s) 577, 582
BstKTI GATC 3 cut(s) 6, 525, 553
BstMBI GATC 3 cut(s) 3, 522, 550
BstMWI GCNNNNNNNGC 2 cut(s) 17, 311
BstNI CCWGG 2 cut(s) 122, 291
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 120, 289
BstSFI CTRYAG 1 cut(s) 595
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 2 cut(s) 577, 582
BstV1I GCAGC 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 1 cut(s) 705
BsuTUI ATCGAT 1 cut(s) 549
BtsCI GGATG 2 cut(s) 130, 373
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 3 cut(s) 160, 261, 562
CfoI GCGC 2 cut(s) 577, 582
Cfr13I GGNCC 3 cut(s) 47, 232, 430
ClaI ATCGAT 1 cut(s) 549
CseI GACGC 1 cut(s) 487
CviAII CATG 1 cut(s) 7
DdeI CTNAG 1 cut(s) 112
DpnI GATC 3 cut(s) 5, 524, 552
DpnII GATC 3 cut(s) 3, 522, 550
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
EciI GGCGGA 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 22
Eco47I GGWCC 3 cut(s) 47, 232, 430
EcoO109I RGGNCCY 2 cut(s) 232, 430
EcoRII CCWGG 2 cut(s) 120, 289
EcoT38I GRGCYC 1 cut(s) 22
FaeI CATG 1 cut(s) 10
FaqI GGGAC 2 cut(s) 443, 526
FatI CATG 1 cut(s) 6
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 380, 578
FokI GGATG 2 cut(s) 137, 380
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 2 cut(s) 380, 578
FspBI CTAG 4 cut(s) 23, 107, 159, 188
GlaI GCGC 2 cut(s) 576, 581
GluI GCNGC 2 cut(s) 380, 578
GsaI CCCAGC 1 cut(s) 661
HaeIII GGCC 1 cut(s) 705
HapII CCGG 1 cut(s) 389
HgaI GACGC 1 cut(s) 487
HhaI GCGC 2 cut(s) 577, 582
Hin1II CATG 1 cut(s) 10
Hin6I GCGC 2 cut(s) 575, 580
HinP1I GCGC 2 cut(s) 575, 580
HindIII AAGCTT 2 cut(s) 257, 691
HinfI GANTC 4 cut(s) 266, 385, 604, 724
HpaII CCGG 1 cut(s) 389
HphI GGTGA 3 cut(s) 373, 583, 719
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 555
Hpy188III TCNNGA 5 cut(s) 145, 335, 389, 520, 670
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 521
HpyAV CCTTC 4 cut(s) 352, 358, 623, 646
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 3 cut(s) 37, 314, 637
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 311
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 1 cut(s) 10
HspAI GCGC 2 cut(s) 575, 580
Kpn2I TCCGGA 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 522, 550
Lsp1109I GCAGC 1 cut(s) 366
MaeI CTAG 4 cut(s) 23, 107, 159, 188
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 526, 589, 721
MalI GATC 3 cut(s) 5, 524, 552
MboI GATC 3 cut(s) 3, 522, 550
MboII GAAGA 2 cut(s) 20, 496
MhlI GDGCHC 2 cut(s) 22, 39
MluCI AATT 5 cut(s) 204, 307, 410, 422, 699
MlyI GAGTC 2 cut(s) 260, 718
MmeI TCCRAC 1 cut(s) 504
MroI TCCGGA 1 cut(s) 388
MseI TTAA 2 cut(s) 95, 645
MslI CAYNNNNRTG 1 cut(s) 42
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 2 cut(s) 122, 291
MteI GCGCNGCGC 1 cut(s) 578
MvaI CCWGG 2 cut(s) 122, 291
MvnI CGCG 2 cut(s) 577, 582
MwoI GCNNNNNNNGC 2 cut(s) 17, 311
NdeII GATC 3 cut(s) 3, 522, 550
NheI GCTAGC 1 cut(s) 158
NlaIII CATG 1 cut(s) 10
NlaIV GGNNCC 2 cut(s) 233, 431
NmuCI GTSAC 3 cut(s) 526, 589, 721
OliI CACNNNNGTG 1 cut(s) 42
PcsI WCGNNNNNNNCGW 2 cut(s) 522, 531
PfeI GAWTC 2 cut(s) 385, 604
PflFI GACNNNGTC 3 cut(s) 463, 497, 515
PflMI CCANNNNNTGG 1 cut(s) 686
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 2 cut(s) 381, 579
PleI GAGTC 2 cut(s) 260, 718
PpsI GAGTC 2 cut(s) 260, 718
PpuMI RGGWCCY 2 cut(s) 232, 430
PshAI GACNNNNGTC 1 cut(s) 230
Psp5II RGGWCCY 2 cut(s) 232, 430
Psp6I CCWGG 2 cut(s) 120, 289
PspFI CCCAGC 1 cut(s) 657
PspGI CCWGG 2 cut(s) 120, 289
PspN4I GGNNCC 2 cut(s) 233, 431
PspPI GGNCC 3 cut(s) 47, 232, 430
PspPPI RGGWCCY 2 cut(s) 232, 430
PsyI GACNNNGTC 3 cut(s) 463, 497, 515
RseI CAYNNNNRTG 1 cut(s) 42
SaqAI TTAA 2 cut(s) 95, 645
SatI GCNGC 2 cut(s) 380, 578
Sau3AI GATC 3 cut(s) 3, 522, 550
Sau96I GGNCC 3 cut(s) 47, 232, 430
SchI GAGTC 2 cut(s) 260, 718
ScrFI CCNGG 2 cut(s) 122, 291
SduI GDGCHC 2 cut(s) 22, 39
SfcI CTRYAG 1 cut(s) 595
SinI GGWCC 3 cut(s) 47, 232, 430
SmiMI CAYNNNNRTG 1 cut(s) 42
SpeI ACTAGT 1 cut(s) 187
Sse9I AATT 5 cut(s) 204, 307, 410, 422, 699
SsiI CCGC 3 cut(s) 474, 577, 582
SspMI CTAG 4 cut(s) 23, 107, 159, 188
StyD4I CCNGG 2 cut(s) 120, 289
TaiI ACGT 1 cut(s) 645
TaqI TCGA 2 cut(s) 549, 669
TasI AATT 5 cut(s) 204, 307, 410, 422, 699
TauI GCSGC 1 cut(s) 580
TfiI GAWTC 2 cut(s) 385, 604
Tru1I TTAA 2 cut(s) 95, 645
Tru9I TTAA 2 cut(s) 95, 645
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 3 cut(s) 526, 589, 721
TseI GCWGC 1 cut(s) 379
Tsp45I GTSAC 3 cut(s) 526, 589, 721
TspDTI ATGAA 2 cut(s) 99, 558
TspRI CASTG 1 cut(s) 256
Tth111I GACNNNGTC 3 cut(s) 463, 497, 515
Van91I CCANNNNNTGG 1 cut(s) 686
VneI GTGCAC 1 cut(s) 35
VpaK11BI GGWCC 3 cut(s) 47, 232, 430
XapI RAATTY 1 cut(s) 422
XspI CTAG 4 cut(s) 23, 107, 159, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.