RchiOBHm_Chr7g0218071

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017828
Physical Location & Seq
Forward (+)
36017101 .. 36020742
3642 bp
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UTR
Exon/CDS
Intron
PRQ19520

Sequence Viewer

Length: 894 bp
ATGGCTCACGCTCACACCGTTGTTTGCCCCGGTCATGAGCGTTCGATTATGGATCTTTGTTACAGCCCAGATCCTTGTGGTGGTTCTCTTCTGCTCAGTGCTAGCATGGATAGGCGGGCCATTCTGAGAGATGGAGAGTGCGGAGATTGGATTAGAACATTTGCTGAACAAACTTCTTCAGTATGGAATTGCTCTTTGAATATTACTGCAACTGCTTCAACGGCTGCAACTGCCTCTCACTGCAATGCCAAAATTTGGGATCTGCAAACAGGACGGTTGTTGCACATAATTCAAAGCCGCAATCTTGTTCAATCCTGTGCTTTCTCAAAGAATGGGCACAGTTTGCTAACAGGAGGACTCGGCTACACTATCCGTATATTTGATTTGGAACATCCACATGGAAAACCAGAAATCTTACGGGAGTCAAATAAACCTCAGCGAGGATCAGTTCTTACTGCTGCATGGCTCCACAACGATCAGGTCATATTGAGTACATGTAGTCTTCTACAGGGTGTCAGATTATGGGATCGAAGGAGTGGTAAGATACTCCGTACACTCGAGACTGAGTCAATCGTGAGAAGTTCAGAACTGAGTCAAGGTTATATCACCACTGCTGCTGGCTCTACGGTGACATTTTGGGATGAAACCGACTTTAGGTGCGTGAATCGCTACAAAATGCCATGCATGGTACAATCGGCTTCTTTGTATGGGAACAAGTTTATTGCAGGGGGAGATGATGGGCTTGTTCGTTTGTTTGATTTCCATACTGGTGAAGAGATTGAACAATCTAATATGGGTGACTTCGGAACCGTTTTATGTGTGCGTTTCTCGCCTGATGGAAAATCCTATGCCGCGGGATTTGAAGATGGCACTGTTATAATCAAGAAGTTGTAG

Protein Analysis

297

Amino Acids

32.7

Weight (kDa)

6.55

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 5 - 129 2.2e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 185 4.1e-18 WDR5 beta-propeller domain
EIF3I PF24805 9 - 235 1e-17 EIF3I
Beta-prop_THOC3 PF25174 12 - 130 4.2e-08 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 14 - 194 1e-05 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 25 - 225 2e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 52 - 213 1.7e-07 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 81 - 290 1.1e-10 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 101 - 293 7.2e-21 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 102 - 193 1.2e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 153 - 284 2e-09 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 167 - 294 1.1e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 201 - 294 3.9e-06 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 201 - 294 7e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017828)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0218071
rosa_laevigata RLG00000002412 RLG00000002416
rosa_multiflora Rmu_sc0000379.1_g000006 Rmu_sc0002029.1_g000003
rosa_roxburghii Rroxscaffold_3G00241420
rosa_rugosa Rorug07G0173500
rosa_samantha Rh7AG316000 Rh7BG306300 Rh7DG314200 Rh7DG314700
rosa_wichuraiana Rw7G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 878
AccB7I CCANNNNNTGG 1 cut(s) 255
AccII CGCG 1 cut(s) 854
AciI CCGC 5 cut(s) 115, 141, 298, 852, 854
AclWI GGATC 5 cut(s) 60, 65, 267, 451, 534
AcsI RAATTY 1 cut(s) 252
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 3 cut(s) 493, 553, 690
AfiI CCNNNNNNNGG 4 cut(s) 80, 255, 440, 654
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 6 cut(s) 199, 219, 293, 311, 782, 863
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 5 cut(s) 60, 65, 267, 451, 534
Ama87I CYCGRG 1 cut(s) 557
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 3 cut(s) 224, 458, 614
ApoI RAATTY 1 cut(s) 252
AspS9I GGNCC 1 cut(s) 117
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 4 cut(s) 598, 640, 782, 809
AsuNHI GCTAGC 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 494
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 3 cut(s) 211, 445, 601
BccI CCATC 4 cut(s) 125, 731, 830, 860
BceAI ACGGC 1 cut(s) 237
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 506
BisI GCNGC 5 cut(s) 225, 298, 459, 615, 852
BlsI GCNGC 5 cut(s) 226, 299, 460, 616, 853
Bme1390I CCNGG 1 cut(s) 30
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 1 cut(s) 117
BmiI GGNNCC 2 cut(s) 467, 808
BmrFI CCNGG 1 cut(s) 30
BmtI GCTAGC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 28, 852
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 255, 440, 654
Bse1I ACTGG 1 cut(s) 772
Bse3DI GCAATG 1 cut(s) 250
BseDI CCNNGG 2 cut(s) 28, 852
BseGI GGATG 2 cut(s) 391, 646
BseLI CCNNNNNNNGG 4 cut(s) 80, 255, 440, 654
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 5 cut(s) 109, 116, 449, 555, 581
BseNI ACTGG 1 cut(s) 772
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 3 cut(s) 211, 445, 601
Bsh1236I CGCG 1 cut(s) 854
BshFI GGCC 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 1 cut(s) 30
BslI CCNNNNNNNGG 4 cut(s) 80, 255, 440, 654
BsmAI GTCTC 1 cut(s) 554
BsnI GGCC 1 cut(s) 119
BsoBI CYCGRG 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 6 cut(s) 52, 70, 259, 443, 475, 526
BspACI CCGC 5 cut(s) 115, 141, 298, 852, 854
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 5 cut(s) 108, 117, 448, 556, 582
BspFNI CGCG 1 cut(s) 854
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 2 cut(s) 467, 808
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 5 cut(s) 60, 65, 267, 451, 534
BsrDI GCAATG 1 cut(s) 250
BsrI ACTGG 1 cut(s) 772
BssECI CCNNGG 2 cut(s) 28, 852
BssMI GATC 6 cut(s) 52, 70, 259, 443, 475, 526
Bst4CI ACNGT 6 cut(s) 19, 276, 341, 628, 811, 874
Bst6I CTCTTC 2 cut(s) 93, 768
BstAPI GCANNNNNTGC 1 cut(s) 343
BstC8I GCNNGC 3 cut(s) 103, 117, 619
BstDEI CTNAG 5 cut(s) 95, 125, 435, 564, 590
BstDSI CCRYGG 1 cut(s) 852
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 2 cut(s) 391, 646
BstFNI CGCG 1 cut(s) 854
BstKTI GATC 6 cut(s) 55, 73, 262, 446, 478, 529
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 6 cut(s) 52, 70, 259, 443, 475, 526
BstMWI GCNNNNNNNGC 5 cut(s) 221, 230, 343, 666, 829
BstNSI RCATGY 1 cut(s) 498
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 1 cut(s) 506
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 3 cut(s) 211, 445, 601
BstV2I GAAGAC 1 cut(s) 494
BstX2I RGATCY 3 cut(s) 52, 70, 259
BstYI RGATCY 3 cut(s) 52, 70, 259
BsuRI GGCC 1 cut(s) 119
BtgI CCRYGG 1 cut(s) 852
BtsCI GGATG 2 cut(s) 391, 646
BtsI GCAGTG 2 cut(s) 238, 609
BtsIMutI CAGTG 4 cut(s) 103, 238, 609, 870
Cac8I GCNNGC 3 cut(s) 103, 117, 619
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 117
Cfr42I CCGCGG 1 cut(s) 855
Csp6I GTAC 3 cut(s) 492, 552, 689
CviAII CATG 7 cut(s) 35, 106, 398, 462, 495, 681, 685
CviQI GTAC 3 cut(s) 492, 552, 689
DdeI CTNAG 5 cut(s) 95, 125, 435, 564, 590
DpnI GATC 6 cut(s) 54, 72, 261, 445, 477, 528
DpnII GATC 6 cut(s) 52, 70, 259, 443, 475, 526
Eam1104I CTCTTC 2 cut(s) 93, 768
EarI CTCTTC 2 cut(s) 93, 768
Eco57I CTGAAG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 557
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 686
FaeI CATG 7 cut(s) 38, 109, 401, 465, 498, 684, 688
FatI CATG 7 cut(s) 34, 105, 397, 461, 494, 680, 684
FauI CCCGC 2 cut(s) 108, 847
Fnu4HI GCNGC 5 cut(s) 225, 298, 459, 615, 852
FokI GGATG 2 cut(s) 378, 653
Fsp4HI GCNGC 5 cut(s) 225, 298, 459, 615, 852
FspBI CTAG 1 cut(s) 102
GluI GCNGC 5 cut(s) 225, 298, 459, 615, 852
HaeIII GGCC 1 cut(s) 119
HapII CCGG 1 cut(s) 30
Hin1II CATG 7 cut(s) 38, 109, 401, 465, 498, 684, 688
HinfI GANTC 5 cut(s) 357, 422, 566, 592, 664
HpaII CCGG 1 cut(s) 30
HphI GGTGA 4 cut(s) 598, 640, 782, 809
Hpy166II GTNNAC 1 cut(s) 554
Hpy188I TCNGA 4 cut(s) 126, 518, 586, 806
Hpy188III TCNNGA 4 cut(s) 35, 559, 574, 883
Hpy8I GTNNAC 1 cut(s) 554
HpyAV CCTTC 1 cut(s) 525
HpyCH4III ACNGT 6 cut(s) 19, 276, 341, 628, 811, 874
HpyCH4V TGCA 8 cut(s) 209, 227, 243, 265, 283, 461, 684, 725
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 230, 343, 666, 829
HpyF3I CTNAG 5 cut(s) 95, 125, 435, 564, 590
Hsp92II CATG 7 cut(s) 38, 109, 401, 465, 498, 684, 688
KspI CCGCGG 1 cut(s) 855
Kzo9I GATC 6 cut(s) 52, 70, 259, 443, 475, 526
LmnI GCTCC 1 cut(s) 471
Lsp1109I GCAGC 3 cut(s) 211, 445, 601
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 3 cut(s) 59, 628, 797
MalI GATC 6 cut(s) 54, 72, 261, 445, 477, 528
MboI GATC 6 cut(s) 52, 70, 259, 443, 475, 526
MboII GAAGA 5 cut(s) 80, 168, 494, 785, 875
MflI RGATCY 3 cut(s) 52, 70, 259
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 3 cut(s) 187, 252, 288
MlyI GAGTC 4 cut(s) 351, 431, 575, 601
MnlI CCTC 4 cut(s) 244, 347, 434, 444
Mph1103I ATGCAT 1 cut(s) 686
MslI CAYNNNNRTG 3 cut(s) 243, 396, 768
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 854
MwoI GCNNNNNNNGC 5 cut(s) 221, 230, 343, 666, 829
NciI CCSGG 1 cut(s) 30
NdeII GATC 6 cut(s) 52, 70, 259, 443, 475, 526
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 7 cut(s) 38, 109, 401, 465, 498, 684, 688
NlaIV GGNNCC 2 cut(s) 467, 808
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 2 cut(s) 628, 797
NsiI ATGCAT 1 cut(s) 686
NspI RCATGY 1 cut(s) 498
PaeR7I CTCGAG 1 cut(s) 557
PagI TCATGA 1 cut(s) 34
PciI ACATGT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PfeI GAWTC 1 cut(s) 664
PflFI GACNNNGTC 1 cut(s) 565
PflMI CCANNNNNTGG 1 cut(s) 255
PkrI GCNGC 5 cut(s) 226, 299, 460, 616, 853
PleI GAGTC 4 cut(s) 351, 430, 574, 600
PpsI GAGTC 4 cut(s) 351, 430, 574, 600
PscI ACATGT 1 cut(s) 494
PsiI TTATAA 1 cut(s) 878
PspN4I GGNNCC 2 cut(s) 467, 808
PspPI GGNCC 1 cut(s) 117
PsuI RGATCY 3 cut(s) 52, 70, 259
PsyI GACNNNGTC 1 cut(s) 565
RsaI GTAC 3 cut(s) 493, 553, 690
RsaNI GTAC 3 cut(s) 492, 552, 689
RseI CAYNNNNRTG 3 cut(s) 243, 396, 768
SacII CCGCGG 1 cut(s) 855
SatI GCNGC 5 cut(s) 225, 298, 459, 615, 852
Sau3AI GATC 6 cut(s) 52, 70, 259, 443, 475, 526
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 4 cut(s) 351, 431, 575, 601
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 339
SetI ASST 4 cut(s) 436, 483, 601, 659
SfcI CTRYAG 1 cut(s) 506
Sfr274I CTCGAG 1 cut(s) 557
Sfr303I CCGCGG 1 cut(s) 855
SgrBI CCGCGG 1 cut(s) 855
SlaI CTCGAG 1 cut(s) 557
SmiMI CAYNNNNRTG 3 cut(s) 243, 396, 768
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 3 cut(s) 187, 252, 288
SsiI CCGC 5 cut(s) 115, 141, 298, 852, 854
SspI AATATT 1 cut(s) 202
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 28
TaaI ACNGT 6 cut(s) 19, 276, 341, 628, 811, 874
TaqI TCGA 3 cut(s) 44, 529, 558
TasI AATT 3 cut(s) 187, 252, 288
TatI WGTACW 1 cut(s) 491
TauI GCSGC 2 cut(s) 300, 854
TfiI GAWTC 1 cut(s) 664
TscAI CASTG 4 cut(s) 103, 245, 616, 877
TseFI GTSAC 2 cut(s) 628, 797
TseI GCWGC 3 cut(s) 224, 458, 614
Tsp45I GTSAC 2 cut(s) 628, 797
TspDTI ATGAA 1 cut(s) 657
TspGWI ACGGA 2 cut(s) 362, 539
TspRI CASTG 4 cut(s) 103, 245, 616, 877
Tth111I GACNNNGTC 1 cut(s) 565
Van91I CCANNNNNTGG 1 cut(s) 255
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 1 cut(s) 252
XceI RCATGY 1 cut(s) 498
XhoI CTCGAG 1 cut(s) 557
XspI CTAG 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.