Rh7DG314700

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0017828
Physical Location & Seq
Forward (+)
36901590 .. 36905224
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG314700.1

Sequence Viewer

Length: 900 bp
ATGGCCATGGCTCAGGCTCGCACCGTTGTTTGCCCCGGTCATGAGCGTTCGATTATGGATCTTTGTTACAGCCCAGATCCTTGTGGTGGTTCTCTTCTGCTCAGTGCTAGCATGGATAGGCGGGCCATTCTGAGAGATGGAGAGTGCGGAGATTGGATTAGAACATTTGCTGAACAAACTTCTTCAGTATGGAATTGCTCTTTGAATATTACTGCAACAGCTTCAACGGCTGCAACTGCCTCTCACTGCAATGCCAAAATTTGGGATCTGCAAACAGGACGGTTGTTGCACATAATTCAAAGCCGGAATCTTGTTCAATCCTGTGCTTTCTCAAAGAATGGGCACAGTTTGCTAACAGGAGGACTCGGCTACACTATCCGTATATTTGATTTGGATCATCCACATGGAAAACCAGAAATCTTACGGGAGTCAAATAAACCTCAGCGAGGATCAGTTCTTACTGCTGCATGGCTCCACAACGATCAGGTCATATTGAGTACATGTAGTCTTCTACAGGGTGTCAGATTATGGGATCGAAGGAGTGGTAAGATACTCCGTACACTCGAGACTGAGTCAATCGTGAGAAGTTCAGAACTGAGTCAAGGTTATATCACCACTGCTGCTGGCTCTACGGTGACATTTTGGGATGAAACCGACTTTAGGTGCGTGAATCGCTACAAAATGCCATGCATGGTACAATCGGCTTCTTTGTATGGGAACAAGTTTATTGCAGGGGGAGATGATGGGCTTGTTCGTTTGTTTGATTTCCATACTGGTGAAGAGATTGAACAATCTAATATGGGTGACTTCGGAACCGTTTTATGTGTGCGTTTCTCGCCCGATGGAAAATCTTATGCTGCGGGATTTGATGATGGCACTGTTATAATCAAGAAGTTGTAG

Protein Analysis

299

Amino Acids

32.88

Weight (kDa)

6.7

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 7 - 131 1.9e-08 WDR3 first beta-propeller domain
EIF3I PF24805 9 - 237 7.3e-18 EIF3I
Beta-prop_WDR5 PF25175 11 - 191 1.2e-18 WDR5 beta-propeller domain
WD40_Prp19 PF24814 26 - 227 2.1e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 77 - 215 2.1e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 83 - 292 3.6e-11 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 103 - 295 1.1e-20 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 103 - 195 1.4e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 170 - 296 2.6e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 203 - 296 5.6e-06 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 203 - 296 1.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017828)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0218071
rosa_laevigata RLG00000002412 RLG00000002416
rosa_multiflora Rmu_sc0000379.1_g000006 Rmu_sc0002029.1_g000003
rosa_roxburghii Rroxscaffold_3G00241420
rosa_rugosa Rorug07G0173500
rosa_samantha Rh7AG316000 Rh7BG306300 Rh7DG314200 Rh7DG314700
rosa_wichuraiana Rw7G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
AccB7I CCANNNNNTGG 1 cut(s) 261
AciI CCGC 3 cut(s) 121, 147, 860
AclWI GGATC 6 cut(s) 66, 71, 273, 402, 457, 540
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 3 cut(s) 499, 559, 696
AfiI CCNNNNNNNGG 4 cut(s) 86, 261, 446, 660
AflIII ACRYGT 1 cut(s) 500
AgsI TTSAA 5 cut(s) 205, 225, 299, 317, 788
AluBI AGCT 1 cut(s) 221
AluI AGCT 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 6 cut(s) 66, 71, 273, 402, 457, 540
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 2 cut(s) 3, 123
ApeKI GCWGC 4 cut(s) 230, 464, 620, 857
ApoI RAATTY 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 123
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 4 cut(s) 604, 646, 788, 815
AsuNHI GCTAGC 1 cut(s) 107
AvaI CYCGRG 1 cut(s) 563
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 500
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 4 cut(s) 217, 451, 607, 844
BccI CCATC 4 cut(s) 131, 737, 836, 866
BceAI ACGGC 1 cut(s) 243
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 1 cut(s) 108
BfmI CTRYAG 1 cut(s) 512
BisI GCNGC 4 cut(s) 231, 465, 621, 858
BlsI GCNGC 4 cut(s) 232, 466, 622, 859
Bme1390I CCNGG 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 123
BmiI GGNNCC 2 cut(s) 473, 814
BmrFI CCNGG 1 cut(s) 36
BmtI GCTAGC 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 500
Bpu10I CCTNAGC 2 cut(s) 12, 441
BpuMI CCSGG 1 cut(s) 36
BsaBI GATNNNNATC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 6, 34
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 261, 446, 660
Bse1I ACTGG 1 cut(s) 778
Bse3DI GCAATG 1 cut(s) 256
Bse8I GATNNNNATC 1 cut(s) 393
BseDI CCNNGG 2 cut(s) 6, 34
BseGI GGATG 2 cut(s) 397, 652
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 4 cut(s) 86, 261, 446, 660
BseMI GCAATG 1 cut(s) 256
BseMII CTCAG 6 cut(s) 26, 115, 122, 455, 561, 587
BseNI ACTGG 1 cut(s) 778
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 4 cut(s) 217, 451, 607, 844
BshFI GGCC 2 cut(s) 5, 125
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 2 cut(s) 36, 304
BslI CCNNNNNNNGG 4 cut(s) 86, 261, 446, 660
BsmAI GTCTC 1 cut(s) 560
BsnI GGCC 2 cut(s) 5, 125
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 7 cut(s) 58, 76, 265, 394, 449, 481, 532
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 3 cut(s) 121, 147, 860
BspANI GGCC 2 cut(s) 5, 125
BspCNI CTCAG 6 cut(s) 25, 114, 123, 454, 562, 588
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 473, 814
BspOI GCTAGC 1 cut(s) 111
BspPI GGATC 6 cut(s) 66, 71, 273, 402, 457, 540
BsrDI GCAATG 1 cut(s) 256
BsrI ACTGG 1 cut(s) 778
BssECI CCNNGG 2 cut(s) 6, 34
BssMI GATC 7 cut(s) 58, 76, 265, 394, 449, 481, 532
BssT1I CCWWGG 1 cut(s) 6
Bst4CI ACNGT 6 cut(s) 25, 282, 347, 634, 817, 880
Bst6I CTCTTC 2 cut(s) 99, 774
BstAPI GCANNNNNTGC 1 cut(s) 349
BstC8I GCNNGC 4 cut(s) 19, 109, 123, 625
BstDEI CTNAG 6 cut(s) 12, 101, 131, 441, 570, 596
BstDSI CCRYGG 1 cut(s) 6
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 2 cut(s) 397, 652
BstKTI GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 7 cut(s) 58, 76, 265, 394, 449, 481, 532
BstMWI GCNNNNNNNGC 5 cut(s) 227, 236, 349, 672, 835
BstNSI RCATGY 1 cut(s) 504
BstSCI CCNGG 1 cut(s) 34
BstSFI CTRYAG 1 cut(s) 512
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 4 cut(s) 217, 451, 607, 844
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 3 cut(s) 58, 76, 265
BstYI RGATCY 3 cut(s) 58, 76, 265
BsuRI GGCC 2 cut(s) 5, 125
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 2 cut(s) 397, 652
BtsI GCAGTG 2 cut(s) 244, 615
BtsIMutI CAGTG 4 cut(s) 109, 244, 615, 876
Cac8I GCNNGC 4 cut(s) 19, 109, 123, 625
CciI TCATGA 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 123
Csp6I GTAC 3 cut(s) 498, 558, 695
CviAII CATG 8 cut(s) 7, 41, 112, 404, 468, 501, 687, 691
CviQI GTAC 3 cut(s) 498, 558, 695
DdeI CTNAG 6 cut(s) 12, 101, 131, 441, 570, 596
DpnI GATC 7 cut(s) 60, 78, 267, 396, 451, 483, 534
DpnII GATC 7 cut(s) 58, 76, 265, 394, 449, 481, 532
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 99, 774
EarI CTCTTC 2 cut(s) 99, 774
Eco130I CCWWGG 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 563
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 692
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 8 cut(s) 10, 44, 115, 407, 471, 504, 690, 694
FatI CATG 8 cut(s) 6, 40, 111, 403, 467, 500, 686, 690
FauI CCCGC 2 cut(s) 114, 853
Fnu4HI GCNGC 4 cut(s) 231, 465, 621, 858
FokI GGATG 2 cut(s) 384, 659
Fsp4HI GCNGC 4 cut(s) 231, 465, 621, 858
FspBI CTAG 1 cut(s) 108
GluI GCNGC 4 cut(s) 231, 465, 621, 858
HaeIII GGCC 2 cut(s) 5, 125
HapII CCGG 2 cut(s) 36, 304
Hin1II CATG 8 cut(s) 10, 44, 115, 407, 471, 504, 690, 694
HinfI GANTC 6 cut(s) 307, 363, 428, 572, 598, 670
HpaII CCGG 2 cut(s) 36, 304
HphI GGTGA 4 cut(s) 604, 646, 788, 815
Hpy166II GTNNAC 1 cut(s) 560
Hpy188I TCNGA 4 cut(s) 132, 524, 592, 812
Hpy188III TCNNGA 4 cut(s) 41, 565, 580, 889
Hpy8I GTNNAC 1 cut(s) 560
HpyAV CCTTC 1 cut(s) 531
HpyCH4III ACNGT 6 cut(s) 25, 282, 347, 634, 817, 880
HpyCH4V TGCA 8 cut(s) 215, 233, 249, 271, 289, 467, 690, 731
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 236, 349, 672, 835
HpyF3I CTNAG 6 cut(s) 12, 101, 131, 441, 570, 596
Hsp92II CATG 8 cut(s) 10, 44, 115, 407, 471, 504, 690, 694
Kzo9I GATC 7 cut(s) 58, 76, 265, 394, 449, 481, 532
LmnI GCTCC 1 cut(s) 477
Lsp1109I GCAGC 4 cut(s) 217, 451, 607, 844
MaeI CTAG 1 cut(s) 108
MaeIII GTNAC 3 cut(s) 65, 634, 803
MalI GATC 7 cut(s) 60, 78, 267, 396, 451, 483, 534
MboI GATC 7 cut(s) 58, 76, 265, 394, 449, 481, 532
MboII GAAGA 4 cut(s) 86, 174, 500, 791
MflI RGATCY 3 cut(s) 58, 76, 265
MhlI GDGCHC 1 cut(s) 345
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 193, 258, 294
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 357, 437, 581, 607
MnlI CCTC 4 cut(s) 250, 353, 440, 450
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 692
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 249, 402, 774
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 36, 304
MspR9I CCNGG 1 cut(s) 36
MwoI GCNNNNNNNGC 5 cut(s) 227, 236, 349, 672, 835
NciI CCSGG 1 cut(s) 36
NcoI CCATGG 1 cut(s) 6
NdeII GATC 7 cut(s) 58, 76, 265, 394, 449, 481, 532
NheI GCTAGC 1 cut(s) 107
NlaIII CATG 8 cut(s) 10, 44, 115, 407, 471, 504, 690, 694
NlaIV GGNNCC 2 cut(s) 473, 814
NmeAIII GCCGAG 1 cut(s) 345
NmuCI GTSAC 2 cut(s) 634, 803
NsiI ATGCAT 1 cut(s) 692
NspI RCATGY 1 cut(s) 504
PaeR7I CTCGAG 1 cut(s) 563
PagI TCATGA 1 cut(s) 40
PciI ACATGT 1 cut(s) 500
PfeI GAWTC 2 cut(s) 307, 670
PflFI GACNNNGTC 1 cut(s) 571
PflMI CCANNNNNTGG 1 cut(s) 261
PkrI GCNGC 4 cut(s) 232, 466, 622, 859
PleI GAGTC 4 cut(s) 357, 436, 580, 606
PpsI GAGTC 4 cut(s) 357, 436, 580, 606
PscI ACATGT 1 cut(s) 500
PsiI TTATAA 1 cut(s) 884
PspN4I GGNNCC 2 cut(s) 473, 814
PspPI GGNCC 1 cut(s) 123
PsuI RGATCY 3 cut(s) 58, 76, 265
PsyI GACNNNGTC 1 cut(s) 571
RsaI GTAC 3 cut(s) 499, 559, 696
RsaNI GTAC 3 cut(s) 498, 558, 695
RseI CAYNNNNRTG 3 cut(s) 249, 402, 774
SatI GCNGC 4 cut(s) 231, 465, 621, 858
Sau3AI GATC 7 cut(s) 58, 76, 265, 394, 449, 481, 532
Sau96I GGNCC 1 cut(s) 123
SchI GAGTC 4 cut(s) 357, 437, 581, 607
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 1 cut(s) 345
SetI ASST 5 cut(s) 223, 442, 489, 607, 665
SfcI CTRYAG 1 cut(s) 512
Sfr274I CTCGAG 1 cut(s) 563
SlaI CTCGAG 1 cut(s) 563
SmiMI CAYNNNNRTG 3 cut(s) 249, 402, 774
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 3 cut(s) 193, 258, 294
SsiI CCGC 3 cut(s) 121, 147, 860
SspI AATATT 1 cut(s) 208
SspMI CTAG 1 cut(s) 108
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 6 cut(s) 25, 282, 347, 634, 817, 880
TaqI TCGA 3 cut(s) 50, 535, 564
TasI AATT 3 cut(s) 193, 258, 294
TatI WGTACW 1 cut(s) 497
TfiI GAWTC 2 cut(s) 307, 670
TscAI CASTG 4 cut(s) 109, 251, 622, 883
TseFI GTSAC 2 cut(s) 634, 803
TseI GCWGC 4 cut(s) 230, 464, 620, 857
Tsp45I GTSAC 2 cut(s) 634, 803
TspDTI ATGAA 1 cut(s) 663
TspGWI ACGGA 2 cut(s) 368, 545
TspRI CASTG 4 cut(s) 109, 251, 622, 883
Tth111I GACNNNGTC 1 cut(s) 571
Van91I CCANNNNNTGG 1 cut(s) 261
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 1 cut(s) 258
XceI RCATGY 1 cut(s) 504
XhoI CTCGAG 1 cut(s) 563
XspI CTAG 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.