RLG00000002412

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017828
Physical Location & Seq
Reverse (-)
33710688 .. 33714394
3707 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002412

Sequence Viewer

Length: 906 bp
ATGGCCATGGCTCAGGCTCAGGCTCACACCGTTGTTTGCCCCGGTCATGAGCGTTCGATTATGGATCTTTGTTACAGCCCAGATGCTTGTGGTGGTTCTCTTCTGCTCAGTGCTAGCATGGATAGGCGGGCCATTCTGAGAGATGGAGAGTGCGGAGATTGGATTAGAACATTTGCTGAACAAACTTCTTCAGTATGGAATTGCTCTTTGAATATTACTGCAACTGCTTCAACGGCTGCAACTGCCTCTCACTGCAATGCCAAAATTTGGGATCTGCAAACAGGACGGTTGTTGCACATAATTCAAAGCCGGAATCTTGTTCAATCCTGTGCTTTCTCAAAAAATGGGCACAGTTTGCTAACAGGAGGACTCGGCTACACTATCCGTATATTTGATTTGGATCATCCACATGGAAAACCAGAAATCTTCCGGGAGTCAAATAAACCTCAGCGAGGATCAGTTCTTACTGCTGCATGGCTCCACAACGATCAGGTCATATTGAGTACATGTAGTCTTCTACAGGGTGTCAGATTATGGGATCGAAGGAGTGGTAAGATACTCCGTACACTCGAGACTGAGTCAATCGTGAGAAGTTCAGAACTGAGTCAAGGTTATATCACCACTGCTGCTGGCTCTACGGTGACATTTTGGGATGAAACCAACTTTAGGTGCGTGAATCGCTACAAAATGCCATGCATGGTACAATCGGCTTCTTTGTATGGGAACAAGTTTATTGCGGGGGGAAATGATGGGCTTGTTCGTTTGTTTGATTTTCATACTGGTGAAGAGATTGAACAATCTAATATGGGTGACTTCGGAACCGTTTTATGTGTGCGTTTCTCGCCCGATGGAAAATCCTATGCCGCGGGATTTGAAGATGGCACTGTTATAATCAAGAAGTTGTAG

Protein Analysis

302

Amino Acids

33.08

Weight (kDa)

7.08

Isoelectric Point (pI)

51.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 7 - 133 1.2e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 9 - 191 1.1e-18 WDR5 beta-propeller domain
WD40_Prp19 PF24814 12 - 133 3.7e-07 Prp19 WD40 domain
EIF3I PF24805 13 - 239 1.2e-17 EIF3I
Beta-prop_THOC3 PF25174 16 - 134 4.7e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 79 - 217 1.8e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 85 - 294 1.1e-09 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 105 - 297 5.8e-21 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 105 - 198 2.2e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 157 - 291 6.1e-10 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 170 - 298 5.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 205 - 298 2.3e-06 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 205 - 298 8e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017828)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0218071
rosa_laevigata RLG00000002412 RLG00000002416
rosa_multiflora Rmu_sc0000379.1_g000006 Rmu_sc0002029.1_g000003
rosa_roxburghii Rroxscaffold_3G00241420
rosa_rugosa Rorug07G0173500
rosa_samantha Rh7AG316000 Rh7BG306300 Rh7DG314200 Rh7DG314700
rosa_wichuraiana Rw7G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 890
AccB7I CCANNNNNTGG 1 cut(s) 267
AccII CGCG 1 cut(s) 866
AciI CCGC 5 cut(s) 127, 153, 737, 864, 866
AclWI GGATC 5 cut(s) 72, 279, 408, 463, 546
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 264
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 3 cut(s) 505, 565, 702
AfiI CCNNNNNNNGG 3 cut(s) 267, 452, 666
AflIII ACRYGT 1 cut(s) 506
AgsI TTSAA 6 cut(s) 211, 231, 305, 323, 794, 875
Alw26I GTCTC 1 cut(s) 566
AlwI GGATC 5 cut(s) 72, 279, 408, 463, 546
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 2 cut(s) 3, 129
ApeKI GCWGC 3 cut(s) 236, 470, 626
ApoI RAATTY 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 129
AsuC2I CCSGG 2 cut(s) 42, 431
AsuHPI GGTGA 4 cut(s) 610, 652, 794, 821
AsuNHI GCTAGC 1 cut(s) 113
AvaI CYCGRG 1 cut(s) 569
BaeGI GKGCMC 1 cut(s) 351
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 506
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 3 cut(s) 223, 457, 613
BccI CCATC 4 cut(s) 137, 743, 842, 872
BceAI ACGGC 1 cut(s) 249
BcnI CCSGG 2 cut(s) 42, 431
BcoDI GTCTC 1 cut(s) 566
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 518
BisI GCNGC 4 cut(s) 237, 471, 627, 864
BlsI GCNGC 4 cut(s) 238, 472, 628, 865
Bme1390I CCNGG 2 cut(s) 42, 431
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 2 cut(s) 479, 820
BmrFI CCNGG 2 cut(s) 42, 431
BmsI GCATC 1 cut(s) 73
BmtI GCTAGC 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 506
Bpu10I CCTNAGC 3 cut(s) 12, 18, 447
BpuMI CCSGG 2 cut(s) 42, 431
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 3 cut(s) 6, 40, 864
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 452, 666
Bse1I ACTGG 1 cut(s) 784
Bse3DI GCAATG 1 cut(s) 262
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 3 cut(s) 6, 40, 864
BseGI GGATG 2 cut(s) 403, 658
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 3 cut(s) 267, 452, 666
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 7 cut(s) 26, 32, 121, 128, 461, 567, 593
BseNI ACTGG 1 cut(s) 784
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 3 cut(s) 223, 457, 613
Bsh1236I CGCG 1 cut(s) 866
BshFI GGCC 2 cut(s) 5, 131
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 3 cut(s) 42, 310, 430
BslI CCNNNNNNNGG 3 cut(s) 267, 452, 666
BsmAI GTCTC 1 cut(s) 566
BsnI GGCC 2 cut(s) 5, 131
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 6 cut(s) 64, 271, 400, 455, 487, 538
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 5 cut(s) 127, 153, 737, 864, 866
BspANI GGCC 2 cut(s) 5, 131
BspCNI CTCAG 7 cut(s) 25, 31, 120, 129, 460, 568, 594
BspFNI CGCG 1 cut(s) 866
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 479, 820
BspOI GCTAGC 1 cut(s) 117
BspPI GGATC 5 cut(s) 72, 279, 408, 463, 546
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 1 cut(s) 784
BssECI CCNNGG 3 cut(s) 6, 40, 864
BssMI GATC 6 cut(s) 64, 271, 400, 455, 487, 538
BssT1I CCWWGG 1 cut(s) 6
Bst4CI ACNGT 6 cut(s) 31, 288, 353, 640, 823, 886
Bst6I CTCTTC 2 cut(s) 105, 780
BstAPI GCANNNNNTGC 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 115, 129, 631
BstDEI CTNAG 7 cut(s) 12, 18, 107, 137, 447, 576, 602
BstDSI CCRYGG 2 cut(s) 6, 864
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 2 cut(s) 403, 658
BstFNI CGCG 1 cut(s) 866
BstKTI GATC 6 cut(s) 67, 274, 403, 458, 490, 541
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 6 cut(s) 64, 271, 400, 455, 487, 538
BstMWI GCNNNNNNNGC 5 cut(s) 233, 242, 355, 678, 841
BstNSI RCATGY 1 cut(s) 510
BstSCI CCNGG 2 cut(s) 40, 429
BstSFI CTRYAG 1 cut(s) 518
BstSLI GKGCMC 1 cut(s) 351
BstUI CGCG 1 cut(s) 866
BstV1I GCAGC 3 cut(s) 223, 457, 613
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 2 cut(s) 64, 271
BstYI RGATCY 2 cut(s) 64, 271
BsuRI GGCC 2 cut(s) 5, 131
BtgI CCRYGG 2 cut(s) 6, 864
BtsCI GGATG 2 cut(s) 403, 658
BtsI GCAGTG 2 cut(s) 250, 621
BtsIMutI CAGTG 4 cut(s) 115, 250, 621, 882
Cac8I GCNNGC 3 cut(s) 115, 129, 631
CciI TCATGA 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 129
Cfr42I CCGCGG 1 cut(s) 867
Csp6I GTAC 3 cut(s) 504, 564, 701
CviAII CATG 8 cut(s) 7, 47, 118, 410, 474, 507, 693, 697
CviQI GTAC 3 cut(s) 504, 564, 701
DdeI CTNAG 7 cut(s) 12, 18, 107, 137, 447, 576, 602
DpnI GATC 6 cut(s) 66, 273, 402, 457, 489, 540
DpnII GATC 6 cut(s) 64, 271, 400, 455, 487, 538
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 105, 780
EarI CTCTTC 2 cut(s) 105, 780
Eco130I CCWWGG 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 174
Eco88I CYCGRG 1 cut(s) 569
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoT14I CCWWGG 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 698
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 8 cut(s) 10, 50, 121, 413, 477, 510, 696, 700
FatI CATG 8 cut(s) 6, 46, 117, 409, 473, 506, 692, 696
FauI CCCGC 3 cut(s) 120, 730, 859
Fnu4HI GCNGC 4 cut(s) 237, 471, 627, 864
FokI GGATG 2 cut(s) 390, 665
Fsp4HI GCNGC 4 cut(s) 237, 471, 627, 864
FspBI CTAG 1 cut(s) 114
GluI GCNGC 4 cut(s) 237, 471, 627, 864
HaeIII GGCC 2 cut(s) 5, 131
HapII CCGG 3 cut(s) 42, 310, 430
Hin1II CATG 8 cut(s) 10, 50, 121, 413, 477, 510, 696, 700
HinfI GANTC 6 cut(s) 313, 369, 434, 578, 604, 676
HpaII CCGG 3 cut(s) 42, 310, 430
HphI GGTGA 4 cut(s) 610, 652, 794, 821
Hpy166II GTNNAC 1 cut(s) 566
Hpy188I TCNGA 4 cut(s) 138, 530, 598, 818
Hpy188III TCNNGA 4 cut(s) 47, 571, 586, 895
Hpy8I GTNNAC 1 cut(s) 566
HpyAV CCTTC 1 cut(s) 537
HpyCH4III ACNGT 6 cut(s) 31, 288, 353, 640, 823, 886
HpyCH4V TGCA 7 cut(s) 221, 239, 255, 277, 295, 473, 696
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 242, 355, 678, 841
HpyF3I CTNAG 7 cut(s) 12, 18, 107, 137, 447, 576, 602
Hsp92II CATG 8 cut(s) 10, 50, 121, 413, 477, 510, 696, 700
KspI CCGCGG 1 cut(s) 867
Kzo9I GATC 6 cut(s) 64, 271, 400, 455, 487, 538
LmnI GCTCC 1 cut(s) 483
Lsp1109I GCAGC 3 cut(s) 223, 457, 613
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 114
MaeIII GTNAC 3 cut(s) 71, 640, 809
MalI GATC 6 cut(s) 66, 273, 402, 457, 489, 540
MboI GATC 6 cut(s) 64, 271, 400, 455, 487, 538
MboII GAAGA 6 cut(s) 92, 180, 418, 506, 797, 887
MflI RGATCY 2 cut(s) 64, 271
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 199, 264, 300
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 363, 443, 587, 613
MnlI CCTC 4 cut(s) 256, 359, 446, 456
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 698
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 255, 408, 780
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 866
MspI CCGG 3 cut(s) 42, 310, 430
MspR9I CCNGG 2 cut(s) 42, 431
MvnI CGCG 1 cut(s) 866
MwoI GCNNNNNNNGC 5 cut(s) 233, 242, 355, 678, 841
NciI CCSGG 2 cut(s) 42, 431
NcoI CCATGG 1 cut(s) 6
NdeII GATC 6 cut(s) 64, 271, 400, 455, 487, 538
NheI GCTAGC 1 cut(s) 113
NlaIII CATG 8 cut(s) 10, 50, 121, 413, 477, 510, 696, 700
NlaIV GGNNCC 2 cut(s) 479, 820
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 2 cut(s) 640, 809
NsiI ATGCAT 1 cut(s) 698
NspI RCATGY 1 cut(s) 510
PaeR7I CTCGAG 1 cut(s) 569
PagI TCATGA 1 cut(s) 46
PciI ACATGT 1 cut(s) 506
PfeI GAWTC 2 cut(s) 313, 676
PflFI GACNNNGTC 1 cut(s) 577
PflMI CCANNNNNTGG 1 cut(s) 267
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 4 cut(s) 238, 472, 628, 865
PleI GAGTC 4 cut(s) 363, 442, 586, 612
PpsI GAGTC 4 cut(s) 363, 442, 586, 612
PscI ACATGT 1 cut(s) 506
PsiI TTATAA 1 cut(s) 890
PspN4I GGNNCC 2 cut(s) 479, 820
PspPI GGNCC 1 cut(s) 129
PsuI RGATCY 2 cut(s) 64, 271
PsyI GACNNNGTC 1 cut(s) 577
RsaI GTAC 3 cut(s) 505, 565, 702
RsaNI GTAC 3 cut(s) 504, 564, 701
RseI CAYNNNNRTG 3 cut(s) 255, 408, 780
SacII CCGCGG 1 cut(s) 867
SatI GCNGC 4 cut(s) 237, 471, 627, 864
Sau3AI GATC 6 cut(s) 64, 271, 400, 455, 487, 538
Sau96I GGNCC 1 cut(s) 129
SchI GAGTC 4 cut(s) 363, 443, 587, 613
ScrFI CCNGG 2 cut(s) 42, 431
SduI GDGCHC 1 cut(s) 351
SetI ASST 4 cut(s) 448, 495, 613, 671
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 518
Sfr274I CTCGAG 1 cut(s) 569
Sfr303I CCGCGG 1 cut(s) 867
SgrBI CCGCGG 1 cut(s) 867
SlaI CTCGAG 1 cut(s) 569
SmiMI CAYNNNNRTG 3 cut(s) 255, 408, 780
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 3 cut(s) 199, 264, 300
SsiI CCGC 5 cut(s) 127, 153, 737, 864, 866
SspI AATATT 1 cut(s) 214
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 40, 429
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 6 cut(s) 31, 288, 353, 640, 823, 886
TaqI TCGA 3 cut(s) 56, 541, 570
TasI AATT 3 cut(s) 199, 264, 300
TatI WGTACW 1 cut(s) 503
TauI GCSGC 1 cut(s) 866
TfiI GAWTC 2 cut(s) 313, 676
TscAI CASTG 4 cut(s) 115, 257, 628, 889
TseFI GTSAC 2 cut(s) 640, 809
TseI GCWGC 3 cut(s) 236, 470, 626
Tsp45I GTSAC 2 cut(s) 640, 809
TspDTI ATGAA 2 cut(s) 669, 764
TspGWI ACGGA 2 cut(s) 374, 551
TspRI CASTG 4 cut(s) 115, 257, 628, 889
Tth111I GACNNNGTC 1 cut(s) 577
Van91I CCANNNNNTGG 1 cut(s) 267
XagI CCTNNNNNAGG 1 cut(s) 450
XapI RAATTY 1 cut(s) 264
XceI RCATGY 1 cut(s) 510
XhoI CTCGAG 1 cut(s) 569
XspI CTAG 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.