Rorug07G0173500

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0017828
Physical Location & Seq
Forward (+)
14222402 .. 14223756
1355 bp
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UTR
Exon/CDS
Intron
Rorug07G0173500.1

Sequence Viewer

Length: 675 bp
ATGTCGCATAATACCCTAGACTCTAGCTTAGAGTATCCGAGATGTGAAGGAAATATTGAAAAAGCTGTGAAGCTCTTGTTAGGAGTCGCAATCGTACTTTTGTTTGGAAAACCGGATGTGGAAATGGGAGGACTTTTGGCACATGAGATGATGCATGCATGCATGGTACGTTGTACCTTCAAGGATGTAGGCCATGGAAATGGGAAAGAAGCGTTGAAGAAGGTATTTGCCAAAGGTTTTAACTCTTCGCACAAAACCGATGAGCAAGTTCAATATACCAGAAAGTTGAAAGAGATCTTGTCACAGAGGACTGAATGGCGGGAAGACGAAGTTTATGGTCAAGGATTTAGAACTGCTATGCGTGCCATTGAAACATTTGGGTTCAAAACTACGCTGGATCATGCCGTTAAAGATGGAACTCTGCTGGTGGTTCCACCGGTAGTGATGAGGAATAAAACCACCGCTGAGTCAATGAGGAAGAAGACCACTTCAGCTCCAATAACTGAGTTGTTATGGAAGGCGACTAGCAGCACAACTAAATCATTATGGAAGGCCACTGCTGCAACAATTACCAACTTATTATGGAAGGTCACTATCACCTGCGAAACACTGCGATTTGTTACTATATATGGAAGGCCACCGCCACCACCACTACCATCAGGGACAAAGCCATAA

Protein Analysis

224

Amino Acids

24.97

Weight (kDa)

9.49

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 82 - 135 1.2e-07 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017828)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0218071
rosa_laevigata RLG00000002412 RLG00000002416
rosa_multiflora Rmu_sc0000379.1_g000006 Rmu_sc0002029.1_g000003
rosa_roxburghii Rroxscaffold_3G00241420
rosa_rugosa Rorug07G0173500
rosa_samantha Rh7AG316000 Rh7BG306300 Rh7DG314200 Rh7DG314700
rosa_wichuraiana Rw7G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 608
Acc36I ACCTGC 1 cut(s) 608
AciI CCGC 3 cut(s) 319, 462, 641
AclWI GGATC 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 474
AfaI GTAC 3 cut(s) 96, 168, 175
AgeI ACCGGT 1 cut(s) 436
AgsI TTSAA 7 cut(s) 59, 181, 217, 272, 289, 371, 385
AluBI AGCT 4 cut(s) 27, 65, 73, 494
AluI AGCT 4 cut(s) 27, 65, 73, 494
AlwI GGATC 1 cut(s) 405
AoxI GGCC 3 cut(s) 190, 552, 635
ApeKI GCWGC 2 cut(s) 528, 560
AsiGI ACCGGT 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 589
BbsI GAAGAC 2 cut(s) 330, 488
BbvI GCAGC 2 cut(s) 540, 547
BccI CCATC 2 cut(s) 407, 664
BceAI ACGGC 1 cut(s) 389
BciVI GTATCC 1 cut(s) 45
BfaI CTAG 3 cut(s) 17, 24, 525
BfuAI ACCTGC 1 cut(s) 608
BfuI GTATCC 1 cut(s) 45
BglII AGATCT 1 cut(s) 294
BisI GCNGC 2 cut(s) 529, 561
BlsI GCNGC 2 cut(s) 530, 562
BmiI GGNNCC 1 cut(s) 432
BmsI GCATC 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 330, 488
BsaJI CCNNGG 1 cut(s) 193
BsaWI WCCGGW 2 cut(s) 112, 436
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bse118I RCCGGY 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 2 cut(s) 121, 190
BseMII CTCAG 2 cut(s) 456, 495
BseXI GCAGC 2 cut(s) 540, 547
BshFI GGCC 3 cut(s) 192, 554, 637
BshTI ACCGGT 1 cut(s) 436
BsiSI CCGG 2 cut(s) 113, 437
BsnI GGCC 3 cut(s) 192, 554, 637
Bsp143I GATC 2 cut(s) 294, 397
Bsp19I CCATGG 1 cut(s) 193
BspACI CCGC 3 cut(s) 319, 462, 641
BspANI GGCC 3 cut(s) 192, 554, 637
BspCNI CTCAG 2 cut(s) 457, 496
BspLI GGNNCC 1 cut(s) 432
BspMI ACCTGC 1 cut(s) 608
BspPI GGATC 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 436
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 2 cut(s) 294, 397
BssT1I CCWWGG 1 cut(s) 193
Bst6I CTCTTC 1 cut(s) 250
BstC8I GCNNGC 3 cut(s) 156, 160, 363
BstDEI CTNAG 3 cut(s) 28, 465, 504
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 2 cut(s) 121, 190
BstKTI GATC 2 cut(s) 297, 400
BstMBI GATC 2 cut(s) 294, 397
BstMWI GCNNNNNNNGC 2 cut(s) 362, 560
BstNSI RCATGY 2 cut(s) 158, 162
BstV1I GCAGC 2 cut(s) 540, 547
BstV2I GAAGAC 2 cut(s) 330, 488
BstX2I RGATCY 1 cut(s) 294
BstXI CCANNNNNNTGG 1 cut(s) 200
BstYI RGATCY 1 cut(s) 294
BsuI GTATCC 1 cut(s) 45
BsuRI GGCC 3 cut(s) 192, 554, 637
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 2 cut(s) 121, 190
BtsI GCAGTG 2 cut(s) 555, 608
BtsIMutI CAGTG 2 cut(s) 555, 608
BveI ACCTGC 1 cut(s) 608
Cac8I GCNNGC 3 cut(s) 156, 160, 363
Cfr10I RCCGGY 1 cut(s) 436
Csp6I GTAC 3 cut(s) 95, 167, 174
CspAI ACCGGT 1 cut(s) 436
CviAII CATG 6 cut(s) 143, 155, 159, 163, 194, 401
CviJI RGCY 8 cut(s) 27, 65, 73, 192, 494, 554, 637, 670
CviKI_1 RGCY 8 cut(s) 27, 65, 73, 192, 494, 554, 637, 670
CviQI GTAC 3 cut(s) 95, 167, 174
DdeI CTNAG 3 cut(s) 28, 465, 504
DpnI GATC 2 cut(s) 296, 399
DpnII GATC 2 cut(s) 294, 397
Eam1104I CTCTTC 1 cut(s) 250
EarI CTCTTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 193
EcoT22I ATGCAT 3 cut(s) 156, 160, 164
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 6 cut(s) 146, 158, 162, 166, 197, 404
FatI CATG 6 cut(s) 142, 154, 158, 162, 193, 400
FauI CCCGC 1 cut(s) 312
Fnu4HI GCNGC 2 cut(s) 529, 561
FokI GGATG 2 cut(s) 128, 197
Fsp4HI GCNGC 2 cut(s) 529, 561
FspBI CTAG 3 cut(s) 17, 24, 525
GluI GCNGC 2 cut(s) 529, 561
HaeIII GGCC 3 cut(s) 192, 554, 637
HapII CCGG 2 cut(s) 113, 437
Hin1II CATG 6 cut(s) 146, 158, 162, 166, 197, 404
HinfI GANTC 3 cut(s) 20, 84, 467
HpaII CCGG 2 cut(s) 113, 437
HphI GGTGA 1 cut(s) 589
Hpy188I TCNGA 1 cut(s) 39
HpyAV CCTTC 7 cut(s) 41, 187, 214, 511, 544, 580, 627
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 4 cut(s) 154, 158, 162, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 560
HpyF3I CTNAG 3 cut(s) 28, 465, 504
HpySE526I ACGT 1 cut(s) 169
Hsp92II CATG 6 cut(s) 146, 158, 162, 166, 197, 404
Kzo9I GATC 2 cut(s) 294, 397
LmnI GCTCC 1 cut(s) 499
LpnPI CCDG 7 cut(s) 126, 292, 380, 410, 450, 613, 645
Lsp1109I GCAGC 2 cut(s) 540, 547
LweI GCATC 1 cut(s) 141
MaeI CTAG 3 cut(s) 17, 24, 525
MaeII ACGT 1 cut(s) 169
MaeIII GTNAC 3 cut(s) 300, 589, 619
MalI GATC 2 cut(s) 296, 399
MboI GATC 2 cut(s) 294, 397
MboII GAAGA 5 cut(s) 229, 237, 335, 490, 493
MflI RGATCY 1 cut(s) 294
MluCI AATT 1 cut(s) 567
MlyI GAGTC 3 cut(s) 14, 93, 476
MnlI CCTC 4 cut(s) 122, 300, 441, 468
Mph1103I ATGCAT 3 cut(s) 156, 160, 164
MseI TTAA 2 cut(s) 240, 408
MslI CAYNNNNRTG 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 2 cut(s) 113, 437
MwoI GCNNNNNNNGC 2 cut(s) 362, 560
NcoI CCATGG 1 cut(s) 193
NdeII GATC 2 cut(s) 294, 397
NlaIII CATG 6 cut(s) 146, 158, 162, 166, 197, 404
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 2 cut(s) 300, 589
NsiI ATGCAT 3 cut(s) 156, 160, 164
NspI RCATGY 2 cut(s) 158, 162
PaeI GCATGC 2 cut(s) 158, 162
PaqCI CACCTGC 1 cut(s) 608
PinAI ACCGGT 1 cut(s) 436
PkrI GCNGC 2 cut(s) 530, 562
PleI GAGTC 3 cut(s) 14, 92, 475
PpsI GAGTC 3 cut(s) 14, 92, 475
PspN4I GGNNCC 1 cut(s) 432
PsuI RGATCY 1 cut(s) 294
RsaI GTAC 3 cut(s) 96, 168, 175
RsaNI GTAC 3 cut(s) 95, 167, 174
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 2 cut(s) 240, 408
SatI GCNGC 2 cut(s) 529, 561
Sau3AI GATC 2 cut(s) 294, 397
SchI GAGTC 3 cut(s) 14, 93, 476
SfaNI GCATC 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 198
SphI GCATGC 2 cut(s) 158, 162
Sse9I AATT 1 cut(s) 567
SsiI CCGC 3 cut(s) 319, 462, 641
SspI AATATT 1 cut(s) 55
SspMI CTAG 3 cut(s) 17, 24, 525
StyI CCWWGG 1 cut(s) 193
TaiI ACGT 1 cut(s) 172
TasI AATT 1 cut(s) 567
Tru1I TTAA 2 cut(s) 240, 408
Tru9I TTAA 2 cut(s) 240, 408
TscAI CASTG 2 cut(s) 562, 615
TseFI GTSAC 2 cut(s) 300, 589
TseI GCWGC 2 cut(s) 528, 560
Tsp45I GTSAC 2 cut(s) 300, 589
TspRI CASTG 2 cut(s) 562, 615
XceI RCATGY 2 cut(s) 158, 162
XspI CTAG 3 cut(s) 17, 24, 525
Zsp2I ATGCAT 3 cut(s) 156, 160, 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.