Rroxscaffold_3G00241420

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017828
Physical Location & Seq
Reverse (-)
33706760 .. 33711644
4885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00241420.1

Sequence Viewer

Length: 903 bp
ATGGCCGCCATGGCTCAGGCTCGCACCGTTGTTTGCCCCGGTCATGAGCGTTCGATTATGGATCTTTGTTACAGCCCAGATCCTTGTGGTGGTTCTCTTCTGCTCAGTGCTGGCGTGGATAGGCGGGCCATTCTGCGAGATGGAGAGTGCGGAGATTGGATTAGAACATTTGCTGAACAAACTTCTTCAGTATGGAATTGCTCTTTGAATATTACTGCAACTGCTTCAACGGCTGCAACTGCCTCTCACTGCAATGCCAAAATTTGGGATCTGCAAACAGGACGCTTGTTGCACATAATTCAAAGCCGGAATCTTGTTCCATCCTGTGCTTTCTCAAAGAATGGGCACAGTTTGCTAACAGGAGGACTCGGCTACACTATCCGTATATTTGATTTGGATCATCCACATGGAAAACCAGAAATCTTACGGGAGACAAATAAACCTCAGCGAGGATCAGTTCTTACTGCTGCATGGCTCCACAACGATCAGGTCATATTGAGTACATGTAGTCTTCTACAGGGTGTCAGATTATGGGATCGAAGGAGTGGTAAGATACTCCGTACACTCGAGACTGAGTCAATCGTGAGAAGTTCAGAACTGAGTCAAGGTTATATCACCACTGCTGCTGGCTCTACGGTGACATTTTGGGATGAAACCGACTTTAGGTGCGTGAATCGCTACAAAATGCCATGCATGGTACAATCGGCTTCTTTGTATGGGAACAAGTTTGTTGCAGGGGGAAATGATGGGCTTGTTCGTTTGTTTGATTTCCATACTGGTGAAGAGATTGAACAATCTAATATGGGTGACTTCGGAACCGTTTTATGTGTGCGTTTCTCGCCCGATGGAAAATCCTATGCCGCGGGATTTGAAGATGGCACTGTTATAATCAAGAAGTTGTAG

Protein Analysis

300

Amino Acids

32.87

Weight (kDa)

7.05

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 7 - 132 2.6e-07 WDR3 first beta-propeller domain
EIF3I PF24805 10 - 238 9.5e-17 EIF3I
Beta-prop_WDR5 PF25175 12 - 190 5.6e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 26 - 228 5.5e-12 Prp19 WD40 domain
WD40_Gbeta PF25391 84 - 293 8.6e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 87 - 228 9e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 104 - 195 1.7e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 105 - 296 1.2e-20 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 156 - 291 5.7e-10 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 169 - 297 6.2e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 203 - 297 4.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 204 - 297 3e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017828)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0218071
rosa_laevigata RLG00000002412 RLG00000002416
rosa_multiflora Rmu_sc0000379.1_g000006 Rmu_sc0002029.1_g000003
rosa_roxburghii Rroxscaffold_3G00241420
rosa_rugosa Rorug07G0173500
rosa_samantha Rh7AG316000 Rh7BG306300 Rh7DG314200 Rh7DG314700
rosa_wichuraiana Rw7G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 887
AccB7I CCANNNNNTGG 1 cut(s) 264
AccII CGCG 1 cut(s) 863
AciI CCGC 5 cut(s) 6, 124, 150, 861, 863
AclWI GGATC 6 cut(s) 69, 74, 276, 405, 460, 543
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 261
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 3 cut(s) 502, 562, 699
AfiI CCNNNNNNNGG 4 cut(s) 89, 264, 449, 663
AflIII ACRYGT 1 cut(s) 503
AgsI TTSAA 5 cut(s) 208, 228, 302, 791, 872
Alw26I GTCTC 2 cut(s) 425, 563
AlwI GGATC 6 cut(s) 69, 74, 276, 405, 460, 543
Ama87I CYCGRG 1 cut(s) 566
AoxI GGCC 2 cut(s) 3, 126
ApeKI GCWGC 3 cut(s) 233, 467, 623
ApoI RAATTY 1 cut(s) 261
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 4 cut(s) 607, 649, 791, 818
AvaI CYCGRG 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 503
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 3 cut(s) 220, 454, 610
BccI CCATC 5 cut(s) 134, 328, 740, 839, 869
BceAI ACGGC 1 cut(s) 246
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 2 cut(s) 425, 563
BfmI CTRYAG 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 5 cut(s) 6, 234, 468, 624, 861
BlsI GCNGC 5 cut(s) 7, 235, 469, 625, 862
Bme1390I CCNGG 1 cut(s) 39
BmeT110I CYCGRG 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 2 cut(s) 476, 817
BmrFI CCNGG 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 503
Bpu10I CCTNAGC 2 cut(s) 15, 444
BpuMI CCSGG 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 396
BsaJI CCNNGG 3 cut(s) 9, 37, 861
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 264, 449, 663
Bse1I ACTGG 1 cut(s) 781
Bse3DI GCAATG 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 396
BseDI CCNNGG 3 cut(s) 9, 37, 861
BseGI GGATG 3 cut(s) 320, 400, 655
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 4 cut(s) 89, 264, 449, 663
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 5 cut(s) 29, 118, 458, 564, 590
BseNI ACTGG 1 cut(s) 781
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 3 cut(s) 220, 454, 610
Bsh1236I CGCG 1 cut(s) 863
BshFI GGCC 2 cut(s) 5, 128
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 2 cut(s) 39, 307
BslI CCNNNNNNNGG 4 cut(s) 89, 264, 449, 663
BsmAI GTCTC 2 cut(s) 425, 563
BsnI GGCC 2 cut(s) 5, 128
BsoBI CYCGRG 1 cut(s) 566
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 5 cut(s) 6, 124, 150, 861, 863
BspANI GGCC 2 cut(s) 5, 128
BspCNI CTCAG 5 cut(s) 28, 117, 457, 565, 591
BspFNI CGCG 1 cut(s) 863
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 476, 817
BspPI GGATC 6 cut(s) 69, 74, 276, 405, 460, 543
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 1 cut(s) 781
BssECI CCNNGG 3 cut(s) 9, 37, 861
BssMI GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
BssT1I CCWWGG 1 cut(s) 9
Bst4CI ACNGT 5 cut(s) 28, 350, 637, 820, 883
Bst6I CTCTTC 2 cut(s) 102, 777
BstAPI GCANNNNNTGC 1 cut(s) 352
BstC8I GCNNGC 4 cut(s) 22, 112, 126, 628
BstDEI CTNAG 5 cut(s) 15, 104, 444, 573, 599
BstDSI CCRYGG 2 cut(s) 9, 861
BstENI CCTNNNNNAGG 1 cut(s) 447
BstF5I GGATG 3 cut(s) 320, 400, 655
BstFNI CGCG 1 cut(s) 863
BstKTI GATC 7 cut(s) 64, 82, 271, 400, 455, 487, 538
BstMAI GTCTC 2 cut(s) 425, 563
BstMBI GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
BstMWI GCNNNNNNNGC 6 cut(s) 11, 230, 239, 352, 675, 838
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 1 cut(s) 37
BstSFI CTRYAG 1 cut(s) 515
BstSLI GKGCMC 1 cut(s) 348
BstUI CGCG 1 cut(s) 863
BstV1I GCAGC 3 cut(s) 220, 454, 610
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 3 cut(s) 61, 79, 268
BstYI RGATCY 3 cut(s) 61, 79, 268
BsuRI GGCC 2 cut(s) 5, 128
BtgI CCRYGG 2 cut(s) 9, 861
BtsCI GGATG 3 cut(s) 320, 400, 655
BtsI GCAGTG 2 cut(s) 247, 618
BtsIMutI CAGTG 4 cut(s) 112, 247, 618, 879
Cac8I GCNNGC 4 cut(s) 22, 112, 126, 628
CciI TCATGA 1 cut(s) 43
Cfr13I GGNCC 1 cut(s) 126
Cfr42I CCGCGG 1 cut(s) 864
CseI GACGC 1 cut(s) 291
Csp6I GTAC 3 cut(s) 501, 561, 698
CviAII CATG 7 cut(s) 10, 44, 407, 471, 504, 690, 694
CviQI GTAC 3 cut(s) 501, 561, 698
DdeI CTNAG 5 cut(s) 15, 104, 444, 573, 599
DpnI GATC 7 cut(s) 63, 81, 270, 399, 454, 486, 537
DpnII GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 102, 777
EarI CTCTTC 2 cut(s) 102, 777
Eco130I CCWWGG 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 566
EcoNI CCTNNNNNAGG 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 695
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 7 cut(s) 13, 47, 410, 474, 507, 693, 697
FatI CATG 7 cut(s) 9, 43, 406, 470, 503, 689, 693
FauI CCCGC 2 cut(s) 117, 856
Fnu4HI GCNGC 5 cut(s) 6, 234, 468, 624, 861
FokI GGATG 3 cut(s) 307, 387, 662
Fsp4HI GCNGC 5 cut(s) 6, 234, 468, 624, 861
GluI GCNGC 5 cut(s) 6, 234, 468, 624, 861
HaeIII GGCC 2 cut(s) 5, 128
HapII CCGG 2 cut(s) 39, 307
HgaI GACGC 1 cut(s) 291
Hin1II CATG 7 cut(s) 13, 47, 410, 474, 507, 693, 697
HinfI GANTC 5 cut(s) 310, 366, 575, 601, 673
HpaII CCGG 2 cut(s) 39, 307
HphI GGTGA 4 cut(s) 607, 649, 791, 818
Hpy166II GTNNAC 1 cut(s) 563
Hpy188I TCNGA 3 cut(s) 527, 595, 815
Hpy188III TCNNGA 4 cut(s) 44, 568, 583, 892
Hpy8I GTNNAC 1 cut(s) 563
HpyAV CCTTC 1 cut(s) 534
HpyCH4III ACNGT 5 cut(s) 28, 350, 637, 820, 883
HpyCH4V TGCA 8 cut(s) 218, 236, 252, 274, 292, 470, 693, 734
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 230, 239, 352, 675, 838
HpyF3I CTNAG 5 cut(s) 15, 104, 444, 573, 599
Hsp92II CATG 7 cut(s) 13, 47, 410, 474, 507, 693, 697
KspI CCGCGG 1 cut(s) 864
Kzo9I GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 3 cut(s) 220, 454, 610
MaeIII GTNAC 3 cut(s) 68, 637, 806
MalI GATC 7 cut(s) 63, 81, 270, 399, 454, 486, 537
MboI GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
MboII GAAGA 5 cut(s) 89, 177, 503, 794, 884
MflI RGATCY 3 cut(s) 61, 79, 268
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 3 cut(s) 196, 261, 297
MlyI GAGTC 3 cut(s) 360, 584, 610
MnlI CCTC 4 cut(s) 253, 356, 443, 453
Mph1103I ATGCAT 1 cut(s) 695
MslI CAYNNNNRTG 3 cut(s) 252, 405, 777
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 2 cut(s) 39, 307
MspR9I CCNGG 1 cut(s) 39
MvnI CGCG 1 cut(s) 863
MwoI GCNNNNNNNGC 6 cut(s) 11, 230, 239, 352, 675, 838
NciI CCSGG 1 cut(s) 39
NcoI CCATGG 1 cut(s) 9
NdeII GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
NlaIII CATG 7 cut(s) 13, 47, 410, 474, 507, 693, 697
NlaIV GGNNCC 2 cut(s) 476, 817
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 2 cut(s) 637, 806
NsiI ATGCAT 1 cut(s) 695
NspI RCATGY 1 cut(s) 507
PaeR7I CTCGAG 1 cut(s) 566
PagI TCATGA 1 cut(s) 43
PciI ACATGT 1 cut(s) 503
PfeI GAWTC 2 cut(s) 310, 673
PflFI GACNNNGTC 1 cut(s) 574
PflMI CCANNNNNTGG 1 cut(s) 264
PkrI GCNGC 5 cut(s) 7, 235, 469, 625, 862
PleI GAGTC 3 cut(s) 360, 583, 609
PpsI GAGTC 3 cut(s) 360, 583, 609
PscI ACATGT 1 cut(s) 503
PsiI TTATAA 1 cut(s) 887
PspN4I GGNNCC 2 cut(s) 476, 817
PspPI GGNCC 1 cut(s) 126
PsuI RGATCY 3 cut(s) 61, 79, 268
PsyI GACNNNGTC 1 cut(s) 574
RsaI GTAC 3 cut(s) 502, 562, 699
RsaNI GTAC 3 cut(s) 501, 561, 698
RseI CAYNNNNRTG 3 cut(s) 252, 405, 777
SacII CCGCGG 1 cut(s) 864
SatI GCNGC 5 cut(s) 6, 234, 468, 624, 861
Sau3AI GATC 7 cut(s) 61, 79, 268, 397, 452, 484, 535
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 3 cut(s) 360, 584, 610
ScrFI CCNGG 1 cut(s) 39
SduI GDGCHC 1 cut(s) 348
SetI ASST 4 cut(s) 445, 492, 610, 668
SfcI CTRYAG 1 cut(s) 515
Sfr274I CTCGAG 1 cut(s) 566
Sfr303I CCGCGG 1 cut(s) 864
SgrBI CCGCGG 1 cut(s) 864
SlaI CTCGAG 1 cut(s) 566
SmiMI CAYNNNNRTG 3 cut(s) 252, 405, 777
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 3 cut(s) 196, 261, 297
SsiI CCGC 5 cut(s) 6, 124, 150, 861, 863
SspI AATATT 1 cut(s) 211
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 5 cut(s) 28, 350, 637, 820, 883
TaqI TCGA 3 cut(s) 53, 538, 567
TasI AATT 3 cut(s) 196, 261, 297
TatI WGTACW 1 cut(s) 500
TauI GCSGC 2 cut(s) 8, 863
TfiI GAWTC 2 cut(s) 310, 673
TscAI CASTG 4 cut(s) 112, 254, 625, 886
TseFI GTSAC 2 cut(s) 637, 806
TseI GCWGC 3 cut(s) 233, 467, 623
Tsp45I GTSAC 2 cut(s) 637, 806
TspDTI ATGAA 1 cut(s) 666
TspGWI ACGGA 2 cut(s) 371, 548
TspRI CASTG 4 cut(s) 112, 254, 625, 886
Tth111I GACNNNGTC 1 cut(s) 574
Van91I CCANNNNNTGG 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 447
XapI RAATTY 1 cut(s) 261
XceI RCATGY 1 cut(s) 507
XhoI CTCGAG 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.