RLG00000002416

serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017828
Physical Location & Seq
Reverse (-)
33740991 .. 33753071
12081 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002416

Sequence Viewer

Length: 831 bp
ATGGCTCAGGCTCACACCGTTGTTTGCCCCGGTCATGAGCGTTCGATTATGGATCTTTGTTACAGCCCAGATGCTTGTGGTGGTTCTCTTCTGCTCAGTGCTAGCATGGATAGGCGGGCCATTCTGAGAGATGGAGAGTGCGGAGATTCGATTAGAACATTTGCTGAACAAACTTCTTCAGTATGGAATTGCTCTTTGAATATTACTGCAACTGCTTCAACGGCTGCAACTGCCTCTCACTGCAATGCCAAAATTTGGGATCTGCAAACAGGGCGGTTGTTGCACATAATTCAAAGCCGGAATCTTGTTCGATCCTGTGTTTTCTCAAAGAATGGGCACAGTTTGCTAACAGGAGGACTCGGCTACACTATCCGTATATTTGATTTGGATCATCCACATGGAAAACCAGAAATCTTCCGGGAGTCAAATAAACCTCAGCGAGGATCAGTTCTTACTGCTGCATGGCTCCACAACGATCAGGTCATATTGAGTACATGTAGTCTTCTACAGGGTGTCAGATTATGGGATCGAAGGAGTGGTAAGATACTCCGTACACTTGAGACTGAGTCAATCGTGAGAAGTTCAGAACTGAGTCAAGTGAATCGCTACAAGATGCCATGCATGGTACAATCGGCTTCTTTGTATGGGAACAAGTTTATTGCAGGGGGAAATGATGGGCTTGTTCGTTTGTTTGATTTCCATACTGGTGAAGAGATTGCACAATCTAATATGGGTGACTTCGGAACCGTTTTATGTGTGCGTTTCTCGCCTGATGGAAAATCCTATGCCTCGGGATTTGCAGATGGCACTGTTATAATCAAGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
WD40

Protein Analysis

277

Amino Acids

30.22

Weight (kDa)

8.53

Isoelectric Point (pI)

53.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 5 - 129 1.3e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 191 1.2e-18 WDR5 beta-propeller domain
EIF3I PF24805 9 - 164 4.6e-14 EIF3I
WD40_Prp19 PF24814 10 - 129 1.7e-06 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 12 - 273 7.4e-22 THOC3 beta-propeller domain
WD40_Gbeta PF25391 81 - 271 4.6e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 106 - 273 8.1e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 166 - 273 2e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017828)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0218071
rosa_laevigata RLG00000002412 RLG00000002416
rosa_multiflora Rmu_sc0000379.1_g000006 Rmu_sc0002029.1_g000003
rosa_roxburghii Rroxscaffold_3G00241420
rosa_rugosa Rorug07G0173500
rosa_samantha Rh7AG316000 Rh7BG306300 Rh7DG314200 Rh7DG314700
rosa_wichuraiana Rw7G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 815
AccB7I CCANNNNNTGG 1 cut(s) 255
AciI CCGC 3 cut(s) 115, 141, 274
AclWI GGATC 6 cut(s) 60, 267, 306, 396, 451, 534
AcsI RAATTY 1 cut(s) 252
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 3 cut(s) 493, 553, 627
AfiI CCNNNNNNNGG 2 cut(s) 255, 440
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 3 cut(s) 199, 219, 293
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 6 cut(s) 60, 267, 306, 396, 451, 534
Ama87I CYCGRG 1 cut(s) 790
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 2 cut(s) 224, 458
ApoI RAATTY 1 cut(s) 252
AspS9I GGNCC 1 cut(s) 117
AsuC2I CCSGG 2 cut(s) 30, 419
AsuHPI GGTGA 2 cut(s) 719, 746
AsuNHI GCTAGC 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 790
BaeGI GKGCMC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 494
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 2 cut(s) 211, 445
BccI CCATC 4 cut(s) 125, 668, 767, 797
BceAI ACGGC 1 cut(s) 237
BcnI CCSGG 2 cut(s) 30, 419
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 506
BisI GCNGC 2 cut(s) 225, 459
BlsI GCNGC 2 cut(s) 226, 460
Bme1390I CCNGG 2 cut(s) 30, 419
BmeT110I CYCGRG 1 cut(s) 790
BmgT120I GGNCC 1 cut(s) 117
BmiI GGNNCC 2 cut(s) 467, 745
BmrFI CCNGG 2 cut(s) 30, 419
BmsI GCATC 2 cut(s) 61, 603
BmtI GCTAGC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 494
Bpu10I CCTNAGC 2 cut(s) 6, 435
BpuEI CTTGAG 1 cut(s) 578
BpuMI CCSGG 2 cut(s) 30, 419
BsaBI GATNNNNATC 1 cut(s) 387
BsaJI CCNNGG 2 cut(s) 28, 789
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 440
Bse1I ACTGG 1 cut(s) 709
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 387
BseDI CCNNGG 2 cut(s) 28, 789
BseGI GGATG 1 cut(s) 391
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 255, 440
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 6 cut(s) 20, 109, 116, 449, 555, 581
BseNI ACTGG 1 cut(s) 709
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 2 cut(s) 211, 445
BshFI GGCC 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 790
BsiSI CCGG 3 cut(s) 30, 298, 418
BslI CCNNNNNNNGG 2 cut(s) 255, 440
BsmAI GTCTC 1 cut(s) 554
BsnI GGCC 1 cut(s) 119
BsoBI CYCGRG 1 cut(s) 790
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 7 cut(s) 52, 259, 311, 388, 443, 475, 526
BspACI CCGC 3 cut(s) 115, 141, 274
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 6 cut(s) 19, 108, 117, 448, 556, 582
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 2 cut(s) 467, 745
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 6 cut(s) 60, 267, 306, 396, 451, 534
BsrDI GCAATG 1 cut(s) 250
BsrI ACTGG 1 cut(s) 709
BssECI CCNNGG 2 cut(s) 28, 789
BssMI GATC 7 cut(s) 52, 259, 311, 388, 443, 475, 526
Bst4CI ACNGT 4 cut(s) 19, 341, 748, 811
Bst6I CTCTTC 2 cut(s) 93, 705
BstAPI GCANNNNNTGC 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 103, 117
BstDEI CTNAG 6 cut(s) 6, 95, 125, 435, 564, 590
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 7 cut(s) 55, 262, 314, 391, 446, 478, 529
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 7 cut(s) 52, 259, 311, 388, 443, 475, 526
BstMWI GCNNNNNNNGC 6 cut(s) 221, 230, 271, 280, 343, 766
BstNSI RCATGY 1 cut(s) 498
BstSCI CCNGG 2 cut(s) 28, 417
BstSFI CTRYAG 1 cut(s) 506
BstSLI GKGCMC 1 cut(s) 339
BstV1I GCAGC 2 cut(s) 211, 445
BstV2I GAAGAC 1 cut(s) 494
BstX2I RGATCY 2 cut(s) 52, 259
BstYI RGATCY 2 cut(s) 52, 259
BsuRI GGCC 1 cut(s) 119
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 3 cut(s) 103, 238, 807
Cac8I GCNNGC 2 cut(s) 103, 117
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 117
Csp6I GTAC 3 cut(s) 492, 552, 626
CviAII CATG 7 cut(s) 35, 106, 398, 462, 495, 618, 622
CviQI GTAC 3 cut(s) 492, 552, 626
DdeI CTNAG 6 cut(s) 6, 95, 125, 435, 564, 590
DpnI GATC 7 cut(s) 54, 261, 313, 390, 445, 477, 528
DpnII GATC 7 cut(s) 52, 259, 311, 388, 443, 475, 526
Eam1104I CTCTTC 2 cut(s) 93, 705
EarI CTCTTC 2 cut(s) 93, 705
Eco57I CTGAAG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 790
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 623
FaeI CATG 7 cut(s) 38, 109, 401, 465, 498, 621, 625
FatI CATG 7 cut(s) 34, 105, 397, 461, 494, 617, 621
FauI CCCGC 1 cut(s) 108
Fnu4HI GCNGC 2 cut(s) 225, 459
FokI GGATG 1 cut(s) 378
Fsp4HI GCNGC 2 cut(s) 225, 459
FspBI CTAG 1 cut(s) 102
GluI GCNGC 2 cut(s) 225, 459
HaeIII GGCC 1 cut(s) 119
HapII CCGG 3 cut(s) 30, 298, 418
Hin1II CATG 7 cut(s) 38, 109, 401, 465, 498, 621, 625
HinfI GANTC 7 cut(s) 146, 301, 357, 422, 566, 592, 601
HpaII CCGG 3 cut(s) 30, 298, 418
HphI GGTGA 2 cut(s) 719, 746
Hpy166II GTNNAC 1 cut(s) 554
Hpy188I TCNGA 4 cut(s) 126, 518, 586, 743
Hpy188III TCNNGA 4 cut(s) 35, 574, 792, 820
Hpy8I GTNNAC 1 cut(s) 554
HpyAV CCTTC 1 cut(s) 525
HpyCH4III ACNGT 4 cut(s) 19, 341, 748, 811
HpyF10VI GCNNNNNNNGC 6 cut(s) 221, 230, 271, 280, 343, 766
HpyF3I CTNAG 6 cut(s) 6, 95, 125, 435, 564, 590
Hsp92II CATG 7 cut(s) 38, 109, 401, 465, 498, 621, 625
Kzo9I GATC 7 cut(s) 52, 259, 311, 388, 443, 475, 526
LmnI GCTCC 1 cut(s) 471
Lsp1109I GCAGC 2 cut(s) 211, 445
LweI GCATC 2 cut(s) 61, 603
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 2 cut(s) 59, 734
MalI GATC 7 cut(s) 54, 261, 313, 390, 445, 477, 528
MboI GATC 7 cut(s) 52, 259, 311, 388, 443, 475, 526
MboII GAAGA 5 cut(s) 80, 168, 406, 494, 722
MflI RGATCY 2 cut(s) 52, 259
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 3 cut(s) 187, 252, 288
MlyI GAGTC 4 cut(s) 351, 431, 575, 601
MnlI CCTC 5 cut(s) 244, 347, 434, 444, 799
Mph1103I ATGCAT 1 cut(s) 623
MslI CAYNNNNRTG 3 cut(s) 243, 396, 705
MspI CCGG 3 cut(s) 30, 298, 418
MspR9I CCNGG 2 cut(s) 30, 419
MwoI GCNNNNNNNGC 6 cut(s) 221, 230, 271, 280, 343, 766
NciI CCSGG 2 cut(s) 30, 419
NdeII GATC 7 cut(s) 52, 259, 311, 388, 443, 475, 526
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 7 cut(s) 38, 109, 401, 465, 498, 621, 625
NlaIV GGNNCC 2 cut(s) 467, 745
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 734
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 1 cut(s) 498
PagI TCATGA 1 cut(s) 34
PciI ACATGT 1 cut(s) 494
PfeI GAWTC 3 cut(s) 146, 301, 601
PflFI GACNNNGTC 1 cut(s) 565
PflMI CCANNNNNTGG 1 cut(s) 255
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 2 cut(s) 226, 460
PleI GAGTC 4 cut(s) 351, 430, 574, 600
PpsI GAGTC 4 cut(s) 351, 430, 574, 600
PscI ACATGT 1 cut(s) 494
PsiI TTATAA 1 cut(s) 815
PspN4I GGNNCC 2 cut(s) 467, 745
PspPI GGNCC 1 cut(s) 117
PsuI RGATCY 2 cut(s) 52, 259
PsyI GACNNNGTC 1 cut(s) 565
RsaI GTAC 3 cut(s) 493, 553, 627
RsaNI GTAC 3 cut(s) 492, 552, 626
RseI CAYNNNNRTG 3 cut(s) 243, 396, 705
SatI GCNGC 2 cut(s) 225, 459
Sau3AI GATC 7 cut(s) 52, 259, 311, 388, 443, 475, 526
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 4 cut(s) 351, 431, 575, 601
ScrFI CCNGG 2 cut(s) 30, 419
SduI GDGCHC 1 cut(s) 339
SetI ASST 2 cut(s) 436, 483
SfaNI GCATC 2 cut(s) 61, 603
SfcI CTRYAG 1 cut(s) 506
SmiMI CAYNNNNRTG 3 cut(s) 243, 396, 705
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 3 cut(s) 187, 252, 288
SsiI CCGC 3 cut(s) 115, 141, 274
SspI AATATT 1 cut(s) 202
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 2 cut(s) 28, 417
TaaI ACNGT 4 cut(s) 19, 341, 748, 811
TaqI TCGA 4 cut(s) 44, 149, 310, 529
TasI AATT 3 cut(s) 187, 252, 288
TatI WGTACW 1 cut(s) 491
TfiI GAWTC 3 cut(s) 146, 301, 601
TscAI CASTG 3 cut(s) 103, 245, 814
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 2 cut(s) 224, 458
Tsp45I GTSAC 1 cut(s) 734
TspGWI ACGGA 2 cut(s) 362, 539
TspRI CASTG 3 cut(s) 103, 245, 814
Tth111I GACNNNGTC 1 cut(s) 565
Van91I CCANNNNNTGG 1 cut(s) 255
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 1 cut(s) 252
XceI RCATGY 1 cut(s) 498
XspI CTAG 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.