RLG00000001113

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018542
Physical Location & Seq
Reverse (-)
10475845 .. 10476326
482 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001113

Sequence Viewer

Length: 366 bp
ATGGCCGTCTACACCACTGTCAATGGGGACTACTTCGCTGCCCACAAGACCAAGGCTGCTCTTAAGGCATTTCAGCACATGATAGCCACTGGAGTCGCCCCCACCTCCTGCACCTACACTATAATCTTCACAGCTCTAGCGTTACACCTCTCTGATGTCCATTTTGTTGGTCTTCTGCAAGTGGAGCATCCACCCCAACTTCCTCTCCAAAGAGTCAAGGAAGATGTACACGTTTTTGCTGGAGGATGGCAATGTGGAGCTAGCCAAGGAGCATACTTCCCATGGTCCTATGATGGAAGGTTTAAAGGGCAACACAGCTATAAAAAGAAGGGTCCTGAACATTGTATTTCCAAGTTGAAGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

122

Amino Acids

13.35

Weight (kDa)

9.2

Isoelectric Point (pI)

23.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 10 - 47 7e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018542)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr0c11g0499521 RchiOBHm_Chr7g0236001
rosa_laevigata RLG00000001107 RLG00000001113 RLG00000001931 RLG00000030010
rosa_multiflora Rmu_sc0000755.1_g000002
rosa_roxburghii Rroxscaffold_3G00225450
rosa_samantha Rh7DG437900 Rh7DG438300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 228
AflII CTTAAG 1 cut(s) 62
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 1 cut(s) 358
AluBI AGCT 3 cut(s) 134, 260, 318
AluI AGCT 3 cut(s) 134, 260, 318
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 38, 56
AspS9I GGNCC 2 cut(s) 285, 332
AsuNHI GCTAGC 1 cut(s) 260
AvaII GGWCC 2 cut(s) 285, 332
BbsI GAAGAC 1 cut(s) 164
BbvI GCAGC 2 cut(s) 25, 43
BccI CCATC 2 cut(s) 240, 287
BfaI CTAG 2 cut(s) 137, 261
BfrI CTTAAG 1 cut(s) 62
BisI GCNGC 2 cut(s) 39, 57
BlsI GCNGC 2 cut(s) 40, 58
Bme18I GGWCC 2 cut(s) 285, 332
BmgT120I GGNCC 2 cut(s) 285, 332
BmiI GGNNCC 1 cut(s) 333
BmsI GCATC 1 cut(s) 196
BmtI GCTAGC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 164
BpmI CTGGAG 2 cut(s) 111, 261
BsaJI CCNNGG 3 cut(s) 51, 265, 281
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bse1I ACTGG 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 257
BseDI CCNNGG 3 cut(s) 51, 265, 281
BseGI GGATG 2 cut(s) 187, 251
BseMI GCAATG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 94
BseXI GCAGC 2 cut(s) 25, 43
BsgI GTGCAG 1 cut(s) 94
BshFI GGCC 1 cut(s) 5
BslFI GGGAC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 41
BsnI GGCC 1 cut(s) 5
Bsp1407I TGTACA 1 cut(s) 226
Bsp19I CCATGG 1 cut(s) 281
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 1 cut(s) 333
BspOI GCTAGC 1 cut(s) 264
BspTI CTTAAG 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 226
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 3 cut(s) 51, 265, 281
BssT1I CCWWGG 3 cut(s) 51, 265, 281
Bst4CI ACNGT 1 cut(s) 19
BstAFI CTTAAG 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 226
BstC8I GCNNGC 1 cut(s) 262
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 2 cut(s) 187, 251
BstMWI GCNNNNNNNGC 2 cut(s) 65, 184
BstV1I GCAGC 2 cut(s) 25, 43
BstV2I GAAGAC 1 cut(s) 164
BstXI CCANNNNNNTGG 1 cut(s) 167
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 281
BtsCI GGATG 2 cut(s) 187, 251
BtsIMutI CAGTG 2 cut(s) 15, 87
Cac8I GCNNGC 1 cut(s) 262
Cfr13I GGNCC 2 cut(s) 285, 332
Csp6I GTAC 1 cut(s) 227
CviAII CATG 2 cut(s) 79, 282
CviJI RGCY 8 cut(s) 5, 56, 86, 134, 260, 264, 318, 363
CviKI_1 RGCY 8 cut(s) 5, 56, 86, 134, 260, 264, 318, 363
CviQI GTAC 1 cut(s) 227
DraI TTTAAA 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 3 cut(s) 51, 265, 281
Eco47I GGWCC 2 cut(s) 285, 332
EcoO109I RGGNCCY 1 cut(s) 332
EcoT14I CCWWGG 3 cut(s) 51, 265, 281
ErhI CCWWGG 3 cut(s) 51, 265, 281
FaeI CATG 2 cut(s) 82, 285
FaiI YATR 6 cut(s) 80, 122, 274, 283, 291, 321
FaqI GGGAC 1 cut(s) 41
FatI CATG 2 cut(s) 78, 281
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 2 cut(s) 39, 57
FokI GGATG 2 cut(s) 174, 258
Fsp4HI GCNGC 2 cut(s) 39, 57
FspBI CTAG 2 cut(s) 137, 261
GluI GCNGC 2 cut(s) 39, 57
GsuI CTGGAG 2 cut(s) 111, 261
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 2 cut(s) 82, 285
HinfI GANTC 2 cut(s) 93, 213
Hpy166II GTNNAC 2 cut(s) 10, 229
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 1 cut(s) 335
Hpy8I GTNNAC 2 cut(s) 10, 229
HpyAV CCTTC 3 cut(s) 291, 322, 352
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 2 cut(s) 111, 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 184
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 2 cut(s) 82, 285
LmnI GCTCC 3 cut(s) 184, 257, 269
LpnPI CCDG 4 cut(s) 75, 121, 225, 348
Lsp1109I GCAGC 2 cut(s) 25, 43
LweI GCATC 1 cut(s) 196
MaeI CTAG 2 cut(s) 137, 261
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 141
MboII GAAGA 3 cut(s) 118, 164, 233
MlyI GAGTC 2 cut(s) 102, 222
MnlI CCTC 4 cut(s) 115, 158, 213, 236
MseI TTAA 2 cut(s) 63, 303
MspCI CTTAAG 1 cut(s) 62
MwoI GCNNNNNNNGC 2 cut(s) 65, 184
NcoI CCATGG 1 cut(s) 281
NheI GCTAGC 1 cut(s) 260
NlaIII CATG 2 cut(s) 82, 285
NlaIV GGNNCC 1 cut(s) 333
PkrI GCNGC 2 cut(s) 40, 58
PleI GAGTC 2 cut(s) 101, 221
PpsI GAGTC 2 cut(s) 101, 221
PpuMI RGGWCCY 1 cut(s) 332
Psp5II RGGWCCY 1 cut(s) 332
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 2 cut(s) 285, 332
PspPPI RGGWCCY 1 cut(s) 332
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 63, 303
SatI GCNGC 2 cut(s) 39, 57
Sau96I GGNCC 2 cut(s) 285, 332
SchI GAGTC 2 cut(s) 102, 222
SetI ASST 8 cut(s) 107, 116, 136, 150, 234, 262, 302, 320
SfaNI GCATC 1 cut(s) 196
SinI GGWCC 2 cut(s) 285, 332
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SspMI CTAG 2 cut(s) 137, 261
StyI CCWWGG 3 cut(s) 51, 265, 281
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 234
TatI WGTACW 1 cut(s) 226
Tru1I TTAA 2 cut(s) 63, 303
Tru9I TTAA 2 cut(s) 63, 303
TscAI CASTG 2 cut(s) 22, 94
TseI GCWGC 2 cut(s) 38, 56
TspRI CASTG 2 cut(s) 22, 94
Vha464I CTTAAG 1 cut(s) 62
VpaK11BI GGWCC 2 cut(s) 285, 332
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 2 cut(s) 137, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.