RLG00000030010

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018542
Physical Location & Seq
Forward (+)
53300118 .. 53300595
478 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030010

Sequence Viewer

Length: 366 bp
ATGGCAGACGCTAGTTGTCACAATTCTTATGCTGATGACCTCAAGAAACATGTGATGAAGCTCCTTAAGGTTCCCTCTGAGGTAGCCAAACTCCCTGACATGGCCGTTTACACCATTGTCAATGGGGACTACTTCGCTGCCCGCAAGACCACGGCTGCTATAAAGGCCTTTCAGCACATGATAGCCACTGGAGTCGCCCCCACCTCCTGCACCTACACTATAATCTTCACAGCTCTTGCATTACACCTCTCTGATGGCCATTTTGTTGGGTATGCCAAGAAGTACTTTTGTGGAAATCTTGGTTGCTCTATCCCACAGAAGCAATGGACTCGATGTAGATGTATGATGATTTTGAGAAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

122

Amino Acids

13.4

Weight (kDa)

9.32

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 32 - 80 4.4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018542)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr0c11g0499521 RchiOBHm_Chr7g0236001
rosa_laevigata RLG00000001107 RLG00000001113 RLG00000001931 RLG00000030010
rosa_multiflora Rmu_sc0000755.1_g000002
rosa_roxburghii Rroxscaffold_3G00225450
rosa_samantha Rh7DG437900 Rh7DG438300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 142
AcoI YGGCCR 2 cut(s) 102, 256
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 100
AflII CTTAAG 1 cut(s) 65
AflIII ACRYGT 1 cut(s) 49
AluBI AGCT 2 cut(s) 61, 233
AluI AGCT 2 cut(s) 61, 233
AoxI GGCC 3 cut(s) 102, 165, 256
ApeKI GCWGC 2 cut(s) 137, 155
BalI TGGCCA 1 cut(s) 258
BbvI GCAGC 2 cut(s) 124, 142
BccI CCATC 1 cut(s) 248
BceAI ACGGC 2 cut(s) 89, 168
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BfaI CTAG 1 cut(s) 12
BfrI CTTAAG 1 cut(s) 65
BisI GCNGC 2 cut(s) 138, 156
BlsI GCNGC 2 cut(s) 139, 157
BmcAI AGTACT 1 cut(s) 284
BmiI GGNNCC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 193
Bse3DI GCAATG 1 cut(s) 329
BseDI CCNNGG 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 193
BseXI GCAGC 2 cut(s) 124, 142
BsgI GTGCAG 1 cut(s) 193
BshFI GGCC 3 cut(s) 104, 167, 258
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 3 cut(s) 104, 167, 258
BspACI CCGC 1 cut(s) 142
BspANI GGCC 3 cut(s) 104, 167, 258
BspCNI CTCAG 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 72
BspTI CTTAAG 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 329
BsrI ACTGG 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 1 cut(s) 78
BstDSI CCRYGG 1 cut(s) 150
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 53
BstV1I GCAGC 2 cut(s) 124, 142
BstXI CCANNNNNNTGG 1 cut(s) 266
BsuRI GGCC 3 cut(s) 104, 167, 258
BtgI CCRYGG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 142
CseI GACGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 283
CviAII CATG 3 cut(s) 50, 100, 178
CviJI RGCY 8 cut(s) 61, 86, 104, 155, 167, 185, 233, 258
CviKI_1 RGCY 8 cut(s) 61, 86, 104, 155, 167, 185, 233, 258
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 78
EaeI YGGCCR 2 cut(s) 102, 256
Eco147I AGGCCT 1 cut(s) 167
FaeI CATG 3 cut(s) 53, 103, 181
FaiI YATR 9 cut(s) 30, 51, 101, 161, 179, 221, 273, 344, 364
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 140
FatI CATG 3 cut(s) 49, 99, 177
FauI CCCGC 1 cut(s) 149
Fnu4HI GCNGC 2 cut(s) 138, 156
Fsp4HI GCNGC 2 cut(s) 138, 156
FspBI CTAG 1 cut(s) 12
GluI GCNGC 2 cut(s) 138, 156
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 3 cut(s) 104, 167, 258
HgaI GACGC 1 cut(s) 17
Hin1II CATG 3 cut(s) 53, 103, 181
HinfI GANTC 2 cut(s) 192, 328
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 79, 253
Hpy188III TCNNGA 1 cut(s) 43
Hpy8I GTNNAC 1 cut(s) 109
HpyCH4V TGCA 2 cut(s) 210, 239
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 78
Hsp92II CATG 3 cut(s) 53, 103, 181
LmnI GCTCC 1 cut(s) 66
LpnPI CCDG 3 cut(s) 108, 174, 220
Lsp1109I GCAGC 2 cut(s) 124, 142
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 17
MboII GAAGA 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 1 cut(s) 22
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 2 cut(s) 201, 322
MnlI CCTC 5 cut(s) 50, 73, 85, 214, 257
Mox20I TGGCCA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 258
MseI TTAA 1 cut(s) 66
Msp20I TGGCCA 1 cut(s) 258
MspCI CTTAAG 1 cut(s) 65
MwoI GCNNNNNNNGC 1 cut(s) 164
NlaIII CATG 3 cut(s) 53, 103, 181
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 17
NspI RCATGY 1 cut(s) 53
PceI AGGCCT 1 cut(s) 167
PciI ACATGT 1 cut(s) 49
PkrI GCNGC 2 cut(s) 139, 157
PleI GAGTC 2 cut(s) 200, 322
PpsI GAGTC 2 cut(s) 200, 322
PscI ACATGT 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 72
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 2 cut(s) 138, 156
ScaI AGTACT 1 cut(s) 284
SchI GAGTC 2 cut(s) 201, 322
SetI ASST 8 cut(s) 42, 63, 72, 84, 206, 215, 235, 249
SmlI CTYRAG 2 cut(s) 41, 65
SmoI CTYRAG 2 cut(s) 41, 65
Sse9I AATT 1 cut(s) 22
SseBI AGGCCT 1 cut(s) 167
SsiI CCGC 1 cut(s) 142
SspMI CTAG 1 cut(s) 12
StuI AGGCCT 1 cut(s) 167
TaqI TCGA 1 cut(s) 331
TasI AATT 1 cut(s) 22
TatI WGTACW 1 cut(s) 282
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 2 cut(s) 137, 155
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 1 cut(s) 71
TspRI CASTG 1 cut(s) 193
Vha464I CTTAAG 1 cut(s) 65
XceI RCATGY 1 cut(s) 53
XcmI CCANNNNNNNNNTGG 1 cut(s) 321
XspI CTAG 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.