RLG00000001554

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015208
Physical Location & Seq
Reverse (-)
17400777 .. 17402265
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001554

Sequence Viewer

Length: 786 bp
ATGGCTTTCGCTGCTGCTCCTCAGGCCTCACACACTTTAAGCTTACTTGGTAGCCCAAAATCAAGAAGAGCAGCAACAACAACCAGAAACTCGAGCCGCCATGTGAATTATGTTGGTACTTTTCATGTGCATGCATCAACCTCATCGTCACAAGCTGAGGGTCCAAGCAAGCAAGAAAGACCCCCACCGGCGCCGGGTATCGATACAAGAATACACTGGGACAACGAAGATGAAGGCTGGATTGGAGGAAGCAGCAGCAAGACGAATGACAAAACACAACAAGTGGAGCAAGAGCAGCACAGGGATGTCTGGGGAAACAAGTTTGGTGATTTGCTCAATGACTCGGCTGATTCGCACTACCAGTTTTTAGGAGTATCAGCGGAAGCTGATCTGGAAGAAATCAAAGCTGCTTATAGAAGACTATCCAAAGAATACCATCCAGACACCACTTCTCTTCCACTAAAGACTGCATCAGACAAGTTTGTGAGGCTAAGAGAAATTTATGATGTGTTGAGTAATGAAGAGAGCCGCAGATTTTACAACTGGACACTTGCTCAAGAGGCTGCAAGCCGCCAGGCTGAAATGATGAGAATGAGGTTGGAGGATCCTTATGTGAAAGAAGTGGAAACGTATGAACCTATCCCGGACATGGTGGATCGTCTTGGTGGAAGGAACATGGCCCTCGGCGATCAAGCAGTGACAGCCCTTACCATTGATGTTTTCATCATCCTCTTTGCTATTTGTTGTATTGTATATGTGATCGTATTCAAGGAGCCATATTACTAG

Protein Analysis

262

Amino Acids

29.48

Weight (kDa)

5.53

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 119 - 181 5.2e-19 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09350
fragaria_vesca FvH4_5g32300
malus_domestica MD15G1410800.v1.1
prunus_persica Prupe.1G551900_v2.0.a1
pyrus_communis pycom15g36560
rosa_chinensis RchiOBHm_Chr7g0228561
rosa_laevigata RLG00000001554
rosa_multiflora Rmu_sc0006021.1_g000007 Rmu_sc0011334.1_g000002
rosa_roxburghii Rroxscaffold_3G00231070
rosa_rugosa Rorug07G0250500.1
rosa_samantha Rh7AG402600 Rh7BG383500 Rh7CG422000 Rh7DG398400
rosa_wichuraiana Rw7G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 190
AciI CCGC 4 cut(s) 97, 380, 529, 571
AclWI GGATC 3 cut(s) 599, 612, 663
AcsI RAATTY 1 cut(s) 498
AcyI GRCGYC 1 cut(s) 191
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 2 cut(s) 194, 649
AgsI TTSAA 1 cut(s) 769
AjnI CCWGG 1 cut(s) 573
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
AluBI AGCT 4 cut(s) 42, 155, 386, 407
AluI AGCT 4 cut(s) 42, 155, 386, 407
AlwI GGATC 3 cut(s) 599, 612, 663
Ama87I CYCGRG 1 cut(s) 91
AoxI GGCC 2 cut(s) 24, 678
ApeKI GCWGC 8 cut(s) 11, 14, 71, 252, 255, 295, 407, 563
ApoI RAATTY 1 cut(s) 498
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 161, 679
AsuC2I CCSGG 2 cut(s) 195, 644
AsuHPI GGTGA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 1 cut(s) 161
AxyI CCTNAGG 1 cut(s) 21
BamHI GGATCC 1 cut(s) 604
BanI GGYRCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 424
BbvCI CCTCAGC 1 cut(s) 156
BbvI GCAGC 6 cut(s) 83, 264, 267, 307, 394, 550
BccI CCATC 1 cut(s) 444
BciT130I CCWGG 1 cut(s) 575
BcnI CCSGG 2 cut(s) 195, 644
BfaI CTAG 1 cut(s) 784
BfoI RGCGCY 1 cut(s) 194
Bme1390I CCNGG 3 cut(s) 195, 575, 644
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 161, 679
BmiI GGNNCC 4 cut(s) 162, 192, 606, 774
BmrFI CCNGG 3 cut(s) 195, 575, 644
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 2 cut(s) 143, 479
BmuI ACTGGG 1 cut(s) 226
BpiI GAAGAC 1 cut(s) 424
Bpu10I CCTNAGC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 540
BpuMI CCSGG 2 cut(s) 195, 644
Bsa29I ATCGAT 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 682
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 649
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 3 cut(s) 221, 361, 548
Bse21I CCTNAGG 1 cut(s) 21
BseBI CCWGG 1 cut(s) 575
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 1 cut(s) 682
BseGI GGATG 3 cut(s) 310, 436, 726
BseLI CCNNNNNNNGG 2 cut(s) 194, 649
BseMII CTCAG 2 cut(s) 35, 147
BseNI ACTGG 3 cut(s) 221, 361, 548
BseRI GAGGAG 1 cut(s) 9
BseXI GCAGC 6 cut(s) 83, 264, 267, 307, 394, 550
BshFI GGCC 2 cut(s) 26, 680
BshNI GGYRCC 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 3 cut(s) 188, 194, 644
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 194, 649
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 2 cut(s) 26, 680
BsoBI CYCGRG 1 cut(s) 91
Bsp143I GATC 5 cut(s) 388, 604, 655, 688, 759
BspACI CCGC 4 cut(s) 97, 380, 529, 571
BspANI GGCC 2 cut(s) 26, 680
BspCNI CTCAG 2 cut(s) 34, 148
BspDI ATCGAT 1 cut(s) 201
BspLI GGNNCC 4 cut(s) 162, 192, 606, 774
BspPI GGATC 3 cut(s) 599, 612, 663
BspQI GCTCTTC 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 3 cut(s) 221, 361, 548
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 682
BssMI GATC 5 cut(s) 388, 604, 655, 688, 759
BssNI GRCGYC 1 cut(s) 191
Bst2UI CCWGG 1 cut(s) 575
Bst6I CTCTTC 3 cut(s) 61, 459, 516
BstACI GRCGYC 1 cut(s) 191
BstC8I GCNNGC 3 cut(s) 132, 170, 568
BstDEI CTNAG 3 cut(s) 21, 156, 491
BstF5I GGATG 3 cut(s) 310, 436, 726
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 5 cut(s) 391, 607, 658, 691, 762
BstMBI GATC 5 cut(s) 388, 604, 655, 688, 759
BstMWI GCNNNNNNNGC 5 cut(s) 11, 23, 295, 560, 701
BstNI CCWGG 1 cut(s) 575
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 3 cut(s) 193, 573, 642
BstV1I GCAGC 6 cut(s) 83, 264, 267, 307, 394, 550
BstV2I GAAGAC 1 cut(s) 424
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
Bsu15I ATCGAT 1 cut(s) 201
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 2 cut(s) 26, 680
BsuTUI ATCGAT 1 cut(s) 201
BtsCI GGATG 3 cut(s) 310, 436, 726
BtsI GCAGTG 1 cut(s) 702
BtsIMutI CAGTG 2 cut(s) 214, 702
Cac8I GCNNGC 3 cut(s) 132, 170, 568
CfoI GCGC 1 cut(s) 193
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 161, 679
ClaI ATCGAT 1 cut(s) 201
Csp6I GTAC 1 cut(s) 117
CviAII CATG 5 cut(s) 101, 125, 131, 649, 676
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 3 cut(s) 21, 156, 491
DinI GGCGCC 1 cut(s) 192
DpnI GATC 5 cut(s) 390, 606, 657, 690, 761
DpnII GATC 5 cut(s) 388, 604, 655, 688, 759
Eam1104I CTCTTC 3 cut(s) 61, 459, 516
EarI CTCTTC 3 cut(s) 61, 459, 516
Eco147I AGGCCT 1 cut(s) 26
Eco47I GGWCC 1 cut(s) 161
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 573
EcoT22I ATGCAT 1 cut(s) 136
EgeI GGCGCC 1 cut(s) 192
EheI GGCGCC 1 cut(s) 192
FaeI CATG 5 cut(s) 104, 128, 134, 652, 679
FaqI GGGAC 1 cut(s) 233
FatI CATG 5 cut(s) 100, 124, 130, 648, 675
FokI GGATG 3 cut(s) 317, 423, 713
FspBI CTAG 1 cut(s) 784
GlaI GCGC 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 2 cut(s) 26, 680
HapII CCGG 3 cut(s) 188, 194, 644
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 5 cut(s) 104, 128, 134, 652, 679
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 2 cut(s) 341, 350
HpaII CCGG 3 cut(s) 188, 194, 644
HphI GGTGA 1 cut(s) 338
Hpy188I TCNGA 1 cut(s) 475
Hpy188III TCNNGA 4 cut(s) 63, 392, 440, 557
HpyAV CCTTC 2 cut(s) 227, 663
HpyCH4IV ACGT 1 cut(s) 629
HpyCH4V TGCA 4 cut(s) 130, 134, 470, 566
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 23, 295, 560, 701
HpyF3I CTNAG 3 cut(s) 21, 156, 491
HpySE526I ACGT 1 cut(s) 629
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 5 cut(s) 104, 128, 134, 652, 679
HspAI GCGC 1 cut(s) 191
KasI GGCGCC 1 cut(s) 190
Kzo9I GATC 5 cut(s) 388, 604, 655, 688, 759
LguI GCTCTTC 1 cut(s) 61
LmnI GCTCC 3 cut(s) 22, 286, 772
Lsp1109I GCAGC 6 cut(s) 83, 264, 267, 307, 394, 550
LweI GCATC 2 cut(s) 143, 479
MaeI CTAG 1 cut(s) 784
MaeII ACGT 1 cut(s) 629
MaeIII GTNAC 2 cut(s) 147, 697
MalI GATC 5 cut(s) 390, 606, 657, 690, 761
MboI GATC 5 cut(s) 388, 604, 655, 688, 759
MboII GAAGA 6 cut(s) 78, 239, 407, 429, 446, 533
MflI RGATCY 1 cut(s) 604
MluCI AATT 2 cut(s) 106, 498
Mly113I GGCGCC 1 cut(s) 191
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 1 cut(s) 579
Mph1103I ATGCAT 1 cut(s) 136
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 2 cut(s) 129, 303
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 3 cut(s) 188, 194, 644
MspR9I CCNGG 3 cut(s) 195, 575, 644
MvaI CCWGG 1 cut(s) 575
MwoI GCNNNNNNNGC 5 cut(s) 11, 23, 295, 560, 701
NarI GGCGCC 1 cut(s) 191
NciI CCSGG 2 cut(s) 195, 644
NdeII GATC 5 cut(s) 388, 604, 655, 688, 759
NlaIII CATG 5 cut(s) 104, 128, 134, 652, 679
NlaIV GGNNCC 4 cut(s) 162, 192, 606, 774
NmeAIII GCCGAG 2 cut(s) 323, 663
NmuCI GTSAC 2 cut(s) 147, 697
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 1 cut(s) 134
PaeI GCATGC 1 cut(s) 134
PaeR7I CTCGAG 1 cut(s) 91
PceI AGGCCT 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 350
PfoI TCCNGGA 1 cut(s) 642
PleI GAGTC 1 cut(s) 335
PluTI GGCGCC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PspN4I GGNNCC 4 cut(s) 162, 192, 606, 774
PspPI GGNCC 2 cut(s) 161, 679
PspXI VCTCGAGB 1 cut(s) 91
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
RseI CAYNNNNRTG 2 cut(s) 129, 303
SapI GCTCTTC 1 cut(s) 61
SaqAI TTAA 1 cut(s) 38
Sau3AI GATC 5 cut(s) 388, 604, 655, 688, 759
Sau96I GGNCC 2 cut(s) 161, 679
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 195, 575, 644
SetI ASST 8 cut(s) 44, 143, 157, 388, 409, 599, 632, 640
SfaNI GCATC 2 cut(s) 143, 479
SfoI GGCGCC 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 91
SgrAI CRCCGGYG 1 cut(s) 187
SinI GGWCC 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 91
SmiMI CAYNNNNRTG 2 cut(s) 129, 303
SmlI CTYRAG 2 cut(s) 91, 555
SmoI CTYRAG 2 cut(s) 91, 555
SphI GCATGC 1 cut(s) 134
Sse9I AATT 2 cut(s) 106, 498
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 4 cut(s) 97, 380, 529, 571
SspDI GGCGCC 1 cut(s) 190
SspMI CTAG 1 cut(s) 784
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 3 cut(s) 193, 573, 642
TaiI ACGT 1 cut(s) 632
TaqI TCGA 2 cut(s) 92, 201
TasI AATT 2 cut(s) 106, 498
TauI GCSGC 3 cut(s) 99, 531, 573
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 2 cut(s) 221, 702
TseFI GTSAC 2 cut(s) 147, 697
TseI GCWGC 8 cut(s) 11, 14, 71, 252, 255, 295, 407, 563
Tsp45I GTSAC 2 cut(s) 147, 697
TspDTI ATGAA 5 cut(s) 113, 246, 534, 648, 712
TspRI CASTG 2 cut(s) 221, 702
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 1 cut(s) 498
XceI RCATGY 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 91
XspI CTAG 1 cut(s) 784
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.