Rh7CG422000

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0015208
Physical Location & Seq
Forward (+)
54568657 .. 54569887
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG422000.1

Sequence Viewer

Length: 786 bp
ATGGCTTTCGCAGCTGCTCCTCAGGCCTCACACACTTTGAGCTTACTTGGTAGCCCAAAATCAAGAAGAGCAGCAACAACAACCAGAAACTCGAGCCGCCATGTGAATTATGTTGGTACTTTTCATGTGCATGCATCAACCTCATCGTCACAAGCTGAGGGTCCAAGCAAGCGAGAAAGACCCCCACCGGCGCCGGGTATAGATACAAGAATACACTGGGAGAACGAAGATGAAGGCTGGATTGGAGGAAGCAGCAGCAAGACGAATGGCAAAACACAACAAGTGGAGCAAGAGCAGCACAAGGATGTCTGGGGAAACAAGTTTGCTGATTTGCTCGATGACTCGGCTGATTCGCACTACCAGTTTTTAGGAGTATCAGCTGAAGCTGATATAGAAGAAATCAAAGCTGCTTATAGAAGACTGTCCAAAGAGTACCATCCAGACACCACTTCTCTTCCACTAAAGACTGCGTCCGACAAGTTTGTTAGGCTAAGAGAAATTTATGATGTGTTGAGTAACGAAGAGAGCCGCAGATTTTACAATTGGACACTTGCTCAAGAGGCTGCAAGCCGCCAGGCTGAAATGATGAGAATGAGGTTGGAGGATCCTTATGTGAAAGAAGTGGAGACGTACGAACCTATCCCGGACATGGTGGATCGTCTTGGTGGAAGGAACATGGCCCTTGGCGATCAAGCAGTGACAGCCCTTACCATTGATGTTTTCATCATCATCTTTGCTATTTGTTGTATTGTATATGTGATCGTATTCAAGGAGCCATATTACTAG

Protein Analysis

261

Amino Acids

29.45

Weight (kDa)

5.66

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 119 - 181 7.2e-19 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09350
fragaria_vesca FvH4_5g32300
malus_domestica MD15G1410800.v1.1
prunus_persica Prupe.1G551900_v2.0.a1
pyrus_communis pycom15g36560
rosa_chinensis RchiOBHm_Chr7g0228561
rosa_laevigata RLG00000001554
rosa_multiflora Rmu_sc0006021.1_g000007 Rmu_sc0011334.1_g000002
rosa_roxburghii Rroxscaffold_3G00231070
rosa_rugosa Rorug07G0250500.1
rosa_samantha Rh7AG402600 Rh7BG383500 Rh7CG422000 Rh7DG398400
rosa_wichuraiana Rw7G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 190
AciI CCGC 3 cut(s) 97, 529, 571
AclWI GGATC 3 cut(s) 599, 612, 663
AcsI RAATTY 1 cut(s) 498
AcuI CTGAAG 1 cut(s) 402
AcyI GRCGYC 1 cut(s) 191
AfaI GTAC 3 cut(s) 118, 434, 632
AfiI CCNNNNNNNGG 2 cut(s) 194, 649
AgsI TTSAA 1 cut(s) 769
AjnI CCWGG 1 cut(s) 573
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
AluBI AGCT 6 cut(s) 14, 42, 155, 380, 386, 407
AluI AGCT 6 cut(s) 14, 42, 155, 380, 386, 407
Alw26I GTCTC 1 cut(s) 620
AlwI GGATC 3 cut(s) 599, 612, 663
Ama87I CYCGRG 1 cut(s) 91
AoxI GGCC 2 cut(s) 24, 678
ApeKI GCWGC 8 cut(s) 11, 14, 71, 252, 255, 295, 407, 563
ApoI RAATTY 1 cut(s) 498
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 161, 679
AsuC2I CCSGG 2 cut(s) 195, 644
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 1 cut(s) 161
AxyI CCTNAGG 1 cut(s) 21
BamHI GGATCC 1 cut(s) 604
BanI GGYRCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 424
BbvCI CCTCAGC 1 cut(s) 156
BbvI GCAGC 7 cut(s) 23, 83, 264, 267, 307, 394, 550
BccI CCATC 1 cut(s) 444
BciT130I CCWGG 1 cut(s) 575
BcnI CCSGG 2 cut(s) 195, 644
BcoDI GTCTC 1 cut(s) 620
BfaI CTAG 1 cut(s) 784
BfoI RGCGCY 1 cut(s) 194
Bme1390I CCNGG 3 cut(s) 195, 575, 644
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 161, 679
BmiI GGNNCC 4 cut(s) 162, 192, 606, 774
BmrFI CCNGG 3 cut(s) 195, 575, 644
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 1 cut(s) 143
BmuI ACTGGG 1 cut(s) 226
BpiI GAAGAC 1 cut(s) 424
Bpu10I CCTNAGC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 540
BpuMI CCSGG 2 cut(s) 195, 644
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 682
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 649
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 2 cut(s) 221, 361
Bse21I CCTNAGG 1 cut(s) 21
BseBI CCWGG 1 cut(s) 575
BseDI CCNNGG 1 cut(s) 682
BseGI GGATG 2 cut(s) 310, 436
BseLI CCNNNNNNNGG 2 cut(s) 194, 649
BseMII CTCAG 2 cut(s) 35, 147
BseNI ACTGG 2 cut(s) 221, 361
BseRI GAGGAG 1 cut(s) 9
BseXI GCAGC 7 cut(s) 23, 83, 264, 267, 307, 394, 550
BshFI GGCC 2 cut(s) 26, 680
BshNI GGYRCC 1 cut(s) 190
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 3 cut(s) 188, 194, 644
BsiWI CGTACG 1 cut(s) 630
BslI CCNNNNNNNGG 2 cut(s) 194, 649
BsmAI GTCTC 1 cut(s) 620
BsmBI CGTCTC 1 cut(s) 620
BsnI GGCC 2 cut(s) 26, 680
BsoBI CYCGRG 1 cut(s) 91
Bsp143I GATC 4 cut(s) 604, 655, 688, 759
BspACI CCGC 3 cut(s) 97, 529, 571
BspANI GGCC 2 cut(s) 26, 680
BspCNI CTCAG 2 cut(s) 34, 148
BspLI GGNNCC 4 cut(s) 162, 192, 606, 774
BspPI GGATC 3 cut(s) 599, 612, 663
BspQI GCTCTTC 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 2 cut(s) 221, 361
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 682
BssMI GATC 4 cut(s) 604, 655, 688, 759
BssNI GRCGYC 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 682
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 1 cut(s) 423
Bst6I CTCTTC 3 cut(s) 61, 459, 516
BstACI GRCGYC 1 cut(s) 191
BstC8I GCNNGC 3 cut(s) 132, 170, 568
BstDEI CTNAG 3 cut(s) 21, 156, 491
BstF5I GGATG 2 cut(s) 310, 436
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 4 cut(s) 607, 658, 691, 762
BstMAI GTCTC 1 cut(s) 620
BstMBI GATC 4 cut(s) 604, 655, 688, 759
BstMWI GCNNNNNNNGC 5 cut(s) 11, 23, 295, 560, 701
BstNI CCWGG 1 cut(s) 575
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 3 cut(s) 193, 573, 642
BstV1I GCAGC 7 cut(s) 23, 83, 264, 267, 307, 394, 550
BstV2I GAAGAC 1 cut(s) 424
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 2 cut(s) 26, 680
BtsCI GGATG 2 cut(s) 310, 436
BtsI GCAGTG 1 cut(s) 702
BtsIMutI CAGTG 2 cut(s) 214, 702
Cac8I GCNNGC 3 cut(s) 132, 170, 568
CfoI GCGC 1 cut(s) 193
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 161, 679
CseI GACGC 1 cut(s) 459
Csp6I GTAC 3 cut(s) 117, 433, 631
CviAII CATG 5 cut(s) 101, 125, 131, 649, 676
CviQI GTAC 3 cut(s) 117, 433, 631
DdeI CTNAG 3 cut(s) 21, 156, 491
DinI GGCGCC 1 cut(s) 192
DpnI GATC 4 cut(s) 606, 657, 690, 761
DpnII GATC 4 cut(s) 604, 655, 688, 759
Eam1104I CTCTTC 3 cut(s) 61, 459, 516
EarI CTCTTC 3 cut(s) 61, 459, 516
Eco130I CCWWGG 1 cut(s) 682
Eco147I AGGCCT 1 cut(s) 26
Eco47I GGWCC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 402
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 573
EcoT14I CCWWGG 1 cut(s) 682
EcoT22I ATGCAT 1 cut(s) 136
EgeI GGCGCC 1 cut(s) 192
EheI GGCGCC 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 682
Esp3I CGTCTC 1 cut(s) 620
FaeI CATG 5 cut(s) 104, 128, 134, 652, 679
FatI CATG 5 cut(s) 100, 124, 130, 648, 675
FokI GGATG 2 cut(s) 317, 423
FspBI CTAG 1 cut(s) 784
GlaI GCGC 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 2 cut(s) 26, 680
HapII CCGG 3 cut(s) 188, 194, 644
HgaI GACGC 1 cut(s) 459
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 5 cut(s) 104, 128, 134, 652, 679
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HinfI GANTC 2 cut(s) 341, 350
HpaII CCGG 3 cut(s) 188, 194, 644
Hpy188I TCNGA 1 cut(s) 475
Hpy188III TCNNGA 3 cut(s) 63, 440, 557
HpyAV CCTTC 2 cut(s) 227, 663
HpyCH4III ACNGT 1 cut(s) 423
HpyCH4IV ACGT 1 cut(s) 629
HpyCH4V TGCA 3 cut(s) 130, 134, 566
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 23, 295, 560, 701
HpyF3I CTNAG 3 cut(s) 21, 156, 491
HpySE526I ACGT 1 cut(s) 629
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 5 cut(s) 104, 128, 134, 652, 679
HspAI GCGC 1 cut(s) 191
KasI GGCGCC 1 cut(s) 190
Kzo9I GATC 4 cut(s) 604, 655, 688, 759
LguI GCTCTTC 1 cut(s) 61
LmnI GCTCC 3 cut(s) 22, 286, 772
Lsp1109I GCAGC 7 cut(s) 23, 83, 264, 267, 307, 394, 550
LweI GCATC 1 cut(s) 143
MaeI CTAG 1 cut(s) 784
MaeII ACGT 1 cut(s) 629
MaeIII GTNAC 3 cut(s) 147, 515, 697
MalI GATC 4 cut(s) 606, 657, 690, 761
MboI GATC 4 cut(s) 604, 655, 688, 759
MboII GAAGA 6 cut(s) 78, 239, 407, 429, 446, 533
MfeI CAATTG 1 cut(s) 541
MflI RGATCY 1 cut(s) 604
MluCI AATT 3 cut(s) 106, 498, 541
Mly113I GGCGCC 1 cut(s) 191
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 2 cut(s) 498, 579
MnlI CCTC 8 cut(s) 30, 37, 151, 151, 239, 553, 588, 595
Mph1103I ATGCAT 1 cut(s) 136
MslI CAYNNNNRTG 2 cut(s) 129, 303
MspA1I CMGCKG 2 cut(s) 14, 380
MspI CCGG 3 cut(s) 188, 194, 644
MspR9I CCNGG 3 cut(s) 195, 575, 644
MunI CAATTG 1 cut(s) 541
MvaI CCWGG 1 cut(s) 575
MwoI GCNNNNNNNGC 5 cut(s) 11, 23, 295, 560, 701
NarI GGCGCC 1 cut(s) 191
NciI CCSGG 2 cut(s) 195, 644
NdeII GATC 4 cut(s) 604, 655, 688, 759
NlaIII CATG 5 cut(s) 104, 128, 134, 652, 679
NlaIV GGNNCC 4 cut(s) 162, 192, 606, 774
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 2 cut(s) 147, 697
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 1 cut(s) 134
PaeI GCATGC 1 cut(s) 134
PaeR7I CTCGAG 1 cut(s) 91
PceI AGGCCT 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 350
Pfl23II CGTACG 1 cut(s) 630
PflFI GACNNNGTC 1 cut(s) 469
PfoI TCCNGGA 1 cut(s) 642
PleI GAGTC 1 cut(s) 335
PluTI GGCGCC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PspLI CGTACG 1 cut(s) 630
PspN4I GGNNCC 4 cut(s) 162, 192, 606, 774
PspPI GGNCC 2 cut(s) 161, 679
PspXI VCTCGAGB 1 cut(s) 91
PsuI RGATCY 1 cut(s) 604
PsyI GACNNNGTC 1 cut(s) 469
PvuII CAGCTG 2 cut(s) 14, 380
RsaI GTAC 3 cut(s) 118, 434, 632
RsaNI GTAC 3 cut(s) 117, 433, 631
RseI CAYNNNNRTG 2 cut(s) 129, 303
SapI GCTCTTC 1 cut(s) 61
Sau3AI GATC 4 cut(s) 604, 655, 688, 759
Sau96I GGNCC 2 cut(s) 161, 679
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 195, 575, 644
SfaNI GCATC 1 cut(s) 143
SfoI GGCGCC 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 91
SgrAI CRCCGGYG 1 cut(s) 187
SinI GGWCC 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 91
SmiMI CAYNNNNRTG 2 cut(s) 129, 303
SmlI CTYRAG 2 cut(s) 91, 555
SmoI CTYRAG 2 cut(s) 91, 555
SphI GCATGC 1 cut(s) 134
Sse9I AATT 3 cut(s) 106, 498, 541
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 3 cut(s) 97, 529, 571
SspDI GGCGCC 1 cut(s) 190
SspMI CTAG 1 cut(s) 784
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 3 cut(s) 193, 573, 642
StyI CCWWGG 1 cut(s) 682
TaaI ACNGT 1 cut(s) 423
TaiI ACGT 1 cut(s) 632
TaqI TCGA 2 cut(s) 92, 336
TasI AATT 3 cut(s) 106, 498, 541
TauI GCSGC 3 cut(s) 99, 531, 573
TfiI GAWTC 1 cut(s) 350
TscAI CASTG 2 cut(s) 221, 702
TseFI GTSAC 2 cut(s) 147, 697
TseI GCWGC 8 cut(s) 11, 14, 71, 252, 255, 295, 407, 563
Tsp45I GTSAC 2 cut(s) 147, 697
TspDTI ATGAA 3 cut(s) 113, 246, 712
TspRI CASTG 2 cut(s) 221, 702
Tth111I GACNNNGTC 1 cut(s) 469
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 1 cut(s) 498
XceI RCATGY 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 91
XspI CTAG 1 cut(s) 784
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.