RLG00000001790

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018005
Physical Location & Seq
Forward (+)
20804334 .. 20805732
1399 bp
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UTR
Exon/CDS
Intron
RLM00000001790

Sequence Viewer

Length: 366 bp
ATGCCCGACAACCTCTACTACAGCGACAACGAAACCTTGATCTCGATCTCCTATTCCGATAAGGCTGAGAGATTGTTGGCTAAAGCAGCGGGTATGGATCGCAGCGGGTTATGTTTCCGGAAGCGTTTCTGGAGAGGGTATCTACGTGGCTCCAATTTCAGGGTTGCGATTGGGAACAGAACTGCCAAGGGGAAAAAAGAGTTCCGAAAGTATCATGCCGCAGCCATTAATGTGCCTGGCTTATGTCCAGCAACTAGAAACAAGCCGTTTAAAACTCGTGCAACTGGAGCTGGAACTGGACAGGACTGGAAAACAGGTAGCCTATGGAGGCAGTTCATCCTTAATTTGGTGGGGGGACTGGGCTAG

Protein Analysis

122

Amino Acids

13.52

Weight (kDa)

10.41

Isoelectric Point (pI)

26.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018005)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g25810 FvH4_3g25810 FvH4_3g25810
pyrus_communis pycom11g14250
rosa_laevigata RLG00000001790 RLG00000005908 RLG00000029485
rosa_roxburghii Rroxscaffold_3G00241990
rosa_samantha Rh1CG027800 Rh5DG332500
rosa_wichuraiana Rw6G020870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 117
AciI CCGC 3 cut(s) 89, 105, 219
AclWI GGATC 1 cut(s) 105
AfiI CCNNNNNNNGG 2 cut(s) 159, 346
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
AlwI GGATC 1 cut(s) 105
Aor13HI TCCGGA 1 cut(s) 117
ApeKI GCWGC 3 cut(s) 86, 102, 221
AseI ATTAAT 1 cut(s) 228
Asp700I GAANNNNTTC 1 cut(s) 125
BauI CACGAG 1 cut(s) 276
BbvI GCAGC 3 cut(s) 98, 114, 233
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 1 cut(s) 237
BfaI CTAG 2 cut(s) 255, 364
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 4 cut(s) 87, 103, 219, 222
BlsI GCNGC 4 cut(s) 88, 104, 220, 223
Bme1390I CCNGG 1 cut(s) 237
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 237
BpmI CTGGAG 2 cut(s) 151, 306
BsaAI YACGTR 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 117
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 346
Bse1I ACTGG 4 cut(s) 289, 301, 311, 363
Bse8I GATNNNNATC 1 cut(s) 44
BseAI TCCGGA 1 cut(s) 117
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 336
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 2 cut(s) 159, 346
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 4 cut(s) 289, 301, 311, 363
BseXI GCAGC 3 cut(s) 98, 114, 233
BsiSI CCGG 1 cut(s) 118
BslI CCNNNNNNNGG 2 cut(s) 159, 346
Bsp13I TCCGGA 1 cut(s) 117
Bsp143I GATC 3 cut(s) 39, 45, 97
BspACI CCGC 3 cut(s) 89, 105, 219
BspCNI CTCAG 1 cut(s) 58
BspEI TCCGGA 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 151
BspPI GGATC 1 cut(s) 105
BsrI ACTGG 4 cut(s) 289, 301, 311, 363
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 3 cut(s) 39, 45, 97
BssSI CACGAG 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 186
Bst2BI CACGAG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 336
BstKTI GATC 3 cut(s) 42, 48, 100
BstMBI GATC 3 cut(s) 39, 45, 97
BstMWI GCNNNNNNNGC 2 cut(s) 86, 287
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 235
BstSFI CTRYAG 1 cut(s) 19
BstV1I GCAGC 3 cut(s) 98, 114, 233
BtsCI GGATG 1 cut(s) 336
CviAII CATG 1 cut(s) 215
CviJI RGCY 9 cut(s) 65, 80, 150, 224, 240, 265, 290, 321, 363
CviKI_1 RGCY 9 cut(s) 65, 80, 150, 224, 240, 265, 290, 321, 363
DdeI CTNAG 1 cut(s) 66
DpnI GATC 3 cut(s) 41, 47, 99
DpnII GATC 3 cut(s) 39, 45, 97
DraI TTTAAA 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 1 cut(s) 218
FaiI YATR 5 cut(s) 95, 112, 216, 244, 325
FatI CATG 1 cut(s) 214
FauI CCCGC 2 cut(s) 82, 98
Fnu4HI GCNGC 4 cut(s) 87, 103, 219, 222
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 4 cut(s) 87, 103, 219, 222
FspBI CTAG 2 cut(s) 255, 364
GluI GCNGC 4 cut(s) 87, 103, 219, 222
GsuI CTGGAG 2 cut(s) 151, 306
HapII CCGG 1 cut(s) 118
Hin1II CATG 1 cut(s) 218
HpaII CCGG 1 cut(s) 118
Hpy188I TCNGA 2 cut(s) 58, 206
Hpy188III TCNNGA 3 cut(s) 43, 118, 130
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 287
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 1 cut(s) 218
Kpn2I TCCGGA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 39, 45, 97
LmnI GCTCC 2 cut(s) 155, 287
Lsp1109I GCAGC 3 cut(s) 98, 114, 233
MaeI CTAG 2 cut(s) 255, 364
MaeII ACGT 1 cut(s) 145
MalI GATC 3 cut(s) 41, 47, 99
MboI GATC 3 cut(s) 39, 45, 97
MluCI AATT 2 cut(s) 154, 343
MnlI CCTC 3 cut(s) 23, 128, 321
MroI TCCGGA 1 cut(s) 117
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 3 cut(s) 228, 270, 342
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 2 cut(s) 89, 105
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 1 cut(s) 237
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 86, 287
NdeII GATC 3 cut(s) 39, 45, 97
NlaIII CATG 1 cut(s) 218
NlaIV GGNNCC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 125
PkrI GCNGC 4 cut(s) 88, 104, 220, 223
Ppu21I YACGTR 1 cut(s) 146
PshBI ATTAAT 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 3 cut(s) 228, 270, 342
SatI GCNGC 4 cut(s) 87, 103, 219, 222
Sau3AI GATC 3 cut(s) 39, 45, 97
ScrFI CCNGG 1 cut(s) 237
SetI ASST 5 cut(s) 15, 38, 148, 292, 319
SfcI CTRYAG 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 2 cut(s) 154, 343
SsiI CCGC 3 cut(s) 89, 105, 219
SspMI CTAG 2 cut(s) 255, 364
StyD4I CCNGG 1 cut(s) 235
StyI CCWWGG 1 cut(s) 186
TaiI ACGT 1 cut(s) 148
TaqI TCGA 1 cut(s) 44
TasI AATT 2 cut(s) 154, 343
TauI GCSGC 1 cut(s) 221
Tru1I TTAA 3 cut(s) 228, 270, 342
Tru9I TTAA 3 cut(s) 228, 270, 342
TseI GCWGC 3 cut(s) 86, 102, 221
TspDTI ATGAA 1 cut(s) 325
VspI ATTAAT 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 2 cut(s) 255, 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.