FvH4_3g25810

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007736
Physical Location & Seq
Reverse (-)
18652015 .. 18657489
5475 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25810.t1

Sequence Viewer

Length: 828 bp
ATGGTGGTGAAGTGCGAGCGAGAAGAGACCAAAACATCAAACTACAAAAACATGGCTGTGGCACCAGTCAAGGAGACTCCAGTCTTCGCCGAAGTTGACATGGGCGACGACTCATCCTCTCCCACCGTCCGCGCCACCATCGTCCAAGCCTCCACCATCTTCTACGACACTCCCGCCACTCTAGATAAGGCTGACAGATTGCTGGCGGAAGCAGCTGGGTATGGATCGCAGCTGGTTGTGTTTCCTGAAGCATTTGTGGGAGGGTATCCTCGTGGCTCCAATTTCGGGGTTGCCATTGGGAACAGAACCGCTAAGGGGAAAGAAGAGTTCCGAAAGTATCATGCCGCTGCCATTGATGTACCTGGTCCTGAAGTTGACCGACTGGCATCAATGGCTGGAAAATATAAGGTCATCCTGGTAATGGGTGTGATAGAGAAAGAAGGGTATACACTATATTGTACTGTTCTGTTTTTTGATTCTCAAGGTTGCTACCTGGGAAAGCACCGAAAAATAATGCCAACAGCATTGGAGCGAATCATTTGGGGATTTGGAGATGGATCCACAATTCGTGTATTTGATACTCCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAATAAAATGCCACTTTTAAGGACAGCAATGTATGCTAAAGGCATTGAAATATATTGTGCTCCCACAGCTGATTCTAGGGATCTATGGCAAGCATCAATGACCCATATTGCTTTAGAAGCGTCATTATATCACCGTCAGGGACTATACTTGCTGGGCCAAATTATGACGGGGAGGCACTCCTCACAGCAGATCTTGATCTTGGAGATATAG

Protein Analysis

276

Amino Acids

30.27

Weight (kDa)

6.96

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 44 - 249 2.3e-47 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 61
AccB7I CCANNNNNTGG 1 cut(s) 612
AccI GTMKAC 1 cut(s) 446
AccII CGCG 1 cut(s) 132
AciI CCGC 5 cut(s) 130, 174, 206, 309, 345
AclWI GGATC 4 cut(s) 232, 552, 565, 705
AcuI CTGAAG 2 cut(s) 267, 390
AfaI GTAC 2 cut(s) 360, 460
AfiI CCNNNNNNNGG 4 cut(s) 285, 315, 421, 612
AgsI TTSAA 1 cut(s) 665
AjnI CCWGG 3 cut(s) 361, 414, 492
AluBI AGCT 3 cut(s) 215, 232, 686
AluI AGCT 3 cut(s) 215, 232, 686
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 2 cut(s) 20, 68
AlwI GGATC 4 cut(s) 232, 552, 565, 705
AoxI GGCC 1 cut(s) 772
ApeKI GCWGC 4 cut(s) 212, 229, 347, 602
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 2 cut(s) 365, 772
AsuHPI GGTGA 2 cut(s) 19, 740
AvaII GGWCC 1 cut(s) 365
BamHI GGATCC 1 cut(s) 557
BanI GGYRCC 1 cut(s) 61
BauI CACGAG 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 679
BbvI GCAGC 4 cut(s) 224, 241, 334, 589
BccI CCATC 3 cut(s) 146, 164, 548
BciT130I CCWGG 3 cut(s) 363, 416, 494
BciVI GTATCC 1 cut(s) 276
BcoDI GTCTC 2 cut(s) 20, 68
BfaI CTAG 2 cut(s) 182, 693
BfuI GTATCC 1 cut(s) 276
BglII AGATCT 1 cut(s) 807
BisI GCNGC 5 cut(s) 213, 230, 345, 348, 603
BlsI GCNGC 5 cut(s) 214, 231, 346, 349, 604
Bme1390I CCNGG 3 cut(s) 363, 416, 494
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 365, 772
BmiI GGNNCC 3 cut(s) 63, 277, 559
BmrFI CCNGG 3 cut(s) 363, 416, 494
BmsI GCATC 2 cut(s) 395, 719
BoxI GACNNNNGTC 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 76
BpmI CTGGAG 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 465
BsaBI GATNNNNATC 1 cut(s) 812
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 493
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 315, 421, 612
Bse1I ACTGG 3 cut(s) 65, 80, 387
Bse3DI GCAATG 1 cut(s) 651
Bse8I GATNNNNATC 1 cut(s) 812
BseBI CCWGG 3 cut(s) 363, 416, 494
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 2 cut(s) 113, 411
BseJI GATNNNNATC 1 cut(s) 812
BseLI CCNNNNNNNGG 4 cut(s) 285, 315, 421, 612
BseMI GCAATG 1 cut(s) 651
BseNI ACTGG 3 cut(s) 65, 80, 387
BseRI GAGGAG 1 cut(s) 787
BseXI GCAGC 4 cut(s) 224, 241, 334, 589
BseYI CCCAGC 2 cut(s) 215, 769
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 774
BshNI GGYRCC 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 679
BslFI GGGAC 1 cut(s) 771
BslI CCNNNNNNNGG 4 cut(s) 285, 315, 421, 612
BsmAI GTCTC 2 cut(s) 20, 68
BsmFI GGGAC 1 cut(s) 771
BsnI GGCC 1 cut(s) 774
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 679
Bsp143I GATC 5 cut(s) 224, 557, 697, 807, 813
BspACI CCGC 5 cut(s) 130, 174, 206, 309, 345
BspANI GGCC 1 cut(s) 774
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 3 cut(s) 63, 277, 559
BspPI GGATC 4 cut(s) 232, 552, 565, 705
BspT107I GGYRCC 1 cut(s) 61
BspTNI GGTCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 3 cut(s) 65, 80, 387
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 5 cut(s) 224, 557, 697, 807, 813
BssNAI GTATAC 1 cut(s) 447
BssSI CACGAG 1 cut(s) 270
Bst1107I GTATAC 1 cut(s) 447
Bst2BI CACGAG 1 cut(s) 270
Bst2UI CCWGG 3 cut(s) 363, 416, 494
Bst4CI ACNGT 3 cut(s) 127, 463, 752
Bst6I CTCTTC 2 cut(s) 18, 318
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 3 cut(s) 17, 204, 708
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 2 cut(s) 113, 411
BstFNI CGCG 1 cut(s) 132
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 5 cut(s) 227, 560, 700, 810, 816
BstMAI GTCTC 2 cut(s) 20, 68
BstMBI GATC 5 cut(s) 224, 557, 697, 807, 813
BstMWI GCNNNNNNNGC 5 cut(s) 212, 392, 650, 683, 734
BstNI CCWGG 3 cut(s) 363, 416, 494
BstPAI GACNNNNGTC 1 cut(s) 80
BstSCI CCNGG 3 cut(s) 361, 414, 492
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 4 cut(s) 224, 241, 334, 589
BstV2I GAAGAC 1 cut(s) 76
BstX2I RGATCY 3 cut(s) 557, 697, 807
BstYI RGATCY 3 cut(s) 557, 697, 807
BstZ17I GTATAC 1 cut(s) 447
BsuI GTATCC 1 cut(s) 276
BsuRI GGCC 1 cut(s) 774
BtsCI GGATG 2 cut(s) 113, 411
Cac8I GCNNGC 3 cut(s) 17, 204, 708
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 2 cut(s) 365, 772
CseI GACGC 1 cut(s) 726
CsiI ACCWGGT 1 cut(s) 361
Csp6I GTAC 2 cut(s) 359, 459
CviAII CATG 3 cut(s) 52, 100, 341
CviJI RGCY 9 cut(s) 56, 149, 191, 215, 232, 276, 395, 686, 774
CviKI_1 RGCY 9 cut(s) 56, 149, 191, 215, 232, 276, 395, 686, 774
CviQI GTAC 2 cut(s) 359, 459
DdeI CTNAG 1 cut(s) 312
DpnI GATC 5 cut(s) 226, 559, 699, 809, 815
DpnII GATC 5 cut(s) 224, 557, 697, 807, 813
Eam1104I CTCTTC 2 cut(s) 18, 318
EarI CTCTTC 2 cut(s) 18, 318
EciI GGCGGA 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 267, 390
EcoRII CCWGG 3 cut(s) 361, 414, 492
FaeI CATG 3 cut(s) 55, 103, 344
FaqI GGGAC 1 cut(s) 771
FatI CATG 3 cut(s) 51, 99, 340
FauI CCCGC 1 cut(s) 181
FblI GTMKAC 1 cut(s) 446
Fnu4HI GCNGC 5 cut(s) 213, 230, 345, 348, 603
FokI GGATG 2 cut(s) 100, 398
Fsp4HI GCNGC 5 cut(s) 213, 230, 345, 348, 603
FspBI CTAG 2 cut(s) 182, 693
GlaI GCGC 1 cut(s) 133
GluI GCNGC 5 cut(s) 213, 230, 345, 348, 603
GsaI CCCAGC 2 cut(s) 219, 773
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 774
HgaI GACGC 1 cut(s) 726
HhaI GCGC 1 cut(s) 134
Hin1II CATG 3 cut(s) 55, 103, 344
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HincII GTYRAC 2 cut(s) 97, 376
HindII GTYRAC 2 cut(s) 97, 376
HinfI GANTC 5 cut(s) 76, 110, 476, 534, 689
HphI GGTGA 2 cut(s) 19, 740
Hpy166II GTNNAC 3 cut(s) 97, 376, 447
Hpy188I TCNGA 1 cut(s) 332
Hpy188III TCNNGA 4 cut(s) 182, 245, 368, 811
Hpy8I GTNNAC 3 cut(s) 97, 376, 447
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 434
HpyCH4III ACNGT 3 cut(s) 127, 463, 752
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 392, 650, 683, 734
HpyF3I CTNAG 1 cut(s) 312
Hsp92II CATG 3 cut(s) 55, 103, 344
HspAI GCGC 1 cut(s) 132
Kzo9I GATC 5 cut(s) 224, 557, 697, 807, 813
LmnI GCTCC 3 cut(s) 281, 529, 682
Lsp1109I GCAGC 4 cut(s) 224, 241, 334, 589
LweI GCATC 2 cut(s) 395, 719
MabI ACCWGGT 1 cut(s) 361
MaeI CTAG 2 cut(s) 182, 693
MalI GATC 5 cut(s) 226, 559, 699, 809, 815
MboI GATC 5 cut(s) 224, 557, 697, 807, 813
MboII GAAGA 4 cut(s) 35, 76, 151, 335
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 3 cut(s) 557, 697, 807
MhlI GDGCHC 1 cut(s) 679
MluCI AATT 4 cut(s) 280, 564, 585, 777
MlyI GAGTC 2 cut(s) 70, 104
MnlI CCTC 6 cut(s) 127, 160, 254, 279, 783, 808
MseI TTAA 1 cut(s) 635
MslI CAYNNNNRTG 1 cut(s) 56
MspA1I CMGCKG 4 cut(s) 215, 232, 347, 686
MspR9I CCNGG 3 cut(s) 363, 416, 494
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 3 cut(s) 363, 416, 494
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 5 cut(s) 212, 392, 650, 683, 734
NdeII GATC 5 cut(s) 224, 557, 697, 807, 813
NlaIII CATG 3 cut(s) 55, 103, 344
NlaIV GGNNCC 3 cut(s) 63, 277, 559
PfeI GAWTC 3 cut(s) 476, 534, 689
PflMI CCANNNNNTGG 1 cut(s) 612
PkrI GCNGC 5 cut(s) 214, 231, 346, 349, 604
PleI GAGTC 2 cut(s) 70, 104
PpsI GAGTC 2 cut(s) 70, 104
PshAI GACNNNNGTC 1 cut(s) 80
Psp6I CCWGG 3 cut(s) 361, 414, 492
PspFI CCCAGC 2 cut(s) 215, 769
PspGI CCWGG 3 cut(s) 361, 414, 492
PspN4I GGNNCC 3 cut(s) 63, 277, 559
PspPI GGNCC 2 cut(s) 365, 772
PsuI RGATCY 3 cut(s) 557, 697, 807
PvuII CAGCTG 3 cut(s) 215, 232, 686
RsaI GTAC 2 cut(s) 360, 460
RsaNI GTAC 2 cut(s) 359, 459
RseI CAYNNNNRTG 1 cut(s) 56
SaqAI TTAA 1 cut(s) 635
SatI GCNGC 5 cut(s) 213, 230, 345, 348, 603
Sau3AI GATC 5 cut(s) 224, 557, 697, 807, 813
Sau96I GGNCC 2 cut(s) 365, 772
SchI GAGTC 2 cut(s) 70, 104
ScrFI CCNGG 3 cut(s) 363, 416, 494
SduI GDGCHC 1 cut(s) 679
SetI ASST 8 cut(s) 217, 234, 364, 411, 487, 495, 601, 688
SexAI ACCWGGT 1 cut(s) 361
SfaNI GCATC 2 cut(s) 395, 719
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 480
SmoI CTYRAG 1 cut(s) 480
Sse9I AATT 4 cut(s) 280, 564, 585, 777
SsiI CCGC 5 cut(s) 130, 174, 206, 309, 345
SspMI CTAG 2 cut(s) 182, 693
StyD4I CCNGG 3 cut(s) 361, 414, 492
TaaI ACNGT 3 cut(s) 127, 463, 752
TaqII GACCGA 1 cut(s) 393
TasI AATT 4 cut(s) 280, 564, 585, 777
TatI WGTACW 1 cut(s) 458
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 3 cut(s) 476, 534, 689
Tru1I TTAA 1 cut(s) 635
Tru9I TTAA 1 cut(s) 635
TseI GCWGC 4 cut(s) 212, 229, 347, 602
Van91I CCANNNNNTGG 1 cut(s) 612
VpaK11BI GGWCC 1 cut(s) 365
XbaI TCTAGA 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 446
XspI CTAG 2 cut(s) 182, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.