Rh5CG347800

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007736
Physical Location & Seq
Reverse (-)
40051072 .. 40057718
6647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG347800.1

Sequence Viewer

Length: 1008 bp
ATGCAATGTGCGAAAGTAAAGACAAAGAGATTCCAGCCTCCAAATTCGAATACAAAAACTGATTGGTCTGAAACTGAGAGAGACCAAATGGCTCTGGTACCCTCATCTGCACCAGTGAACCAGAGCCCAGTGATCACCGAGGTTGACATGGGCGGCGACGACTCCGCACCCACCGTCCAAGCCTCCACCGTCTTCTACGACACTCCCGCCACTCTAGATAAGGCTGAGAGATTGTTGGCTGAAGCAGCTGGGTATGGATCGCAGCTGGTTGTGTTTCCAGAAGCATTTGTGGGAGGGTATCCACGCGGCTCCAATTTCGGGGTTGCGATTGGGAACAGAACTGCCAAGGGGAAAGAAGAGTTCCGAAAGTATCATGCCGCAGCCATTACTGTGCCTGGTCCTGAAGTTGACCGATTGGCATCAATGGCTGGAAAATATAAGGTATTCTTGGTAATGGGTGTGATAGAGAAAGAAGGGTATACACTTTATTGTACTGTTCTGTTTTTTGATTCTCAAGGTTGCTACCTGGGAAAGCACCGAAAAATAATGCCAACAGCATTAGAGCGAATCATTTGGGGATTTGGAGATGGATCCACAATTCCTGTGTTTGATACTCCAATTGGAAAAATAGGTGCTGCCATTTGTTGGGAAAATAAGATGCCACTTTTAAGGACAGCAATGTATGCTAAAGGCATTGAAATATATTGTGCTCCTACAGCTGATTCTAGGGATCTATGGCAAGCGTCAATGACCCATATTGCTTTAGAAGGTGGGTGCTTTGTCTTATCAGCTAACCAATTCTGTCGAAGGAAAGACTATCCCCCTCCACCAGAGTATATATTTTCAGGCACGGAAGAGGAACTTACTCCTGATTCTGTTGTCTGTGCTGGAGGCAGTGTCATCATATCACCGTCAGGGACTATACTTGCTGGGCCCAATTATGACGGGGAGGCACTCATCTCAGCAGATCTTGGTATGCCATGTTTTTATATACTCTCCTATTGTTAA

Protein Analysis

335

Amino Acids

36.32

Weight (kDa)

5.17

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 59 - 324 4.8e-61 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 97
AccB7I CCANNNNNTGG 1 cut(s) 645
AccI GTMKAC 1 cut(s) 479
AccII CGCG 1 cut(s) 306
AciI CCGC 5 cut(s) 153, 165, 207, 306, 378
AclWI GGATC 4 cut(s) 265, 585, 598, 738
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 2 cut(s) 261, 423
AfaI GTAC 2 cut(s) 99, 493
AfiI CCNNNNNNNGG 2 cut(s) 318, 645
AgsI TTSAA 1 cut(s) 698
AjnI CCWGG 2 cut(s) 394, 525
AluBI AGCT 4 cut(s) 248, 265, 719, 791
AluI AGCT 4 cut(s) 248, 265, 719, 791
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 4 cut(s) 265, 585, 598, 738
AoxI GGCC 1 cut(s) 932
ApaI GGGCCC 1 cut(s) 936
ApeKI GCWGC 4 cut(s) 245, 262, 380, 635
ApoI RAATTY 1 cut(s) 43
Asp718I GGTACC 1 cut(s) 97
AspS9I GGNCC 3 cut(s) 398, 932, 933
AsuHPI GGTGA 2 cut(s) 127, 900
AsuII TTCGAA 1 cut(s) 47
AvaII GGWCC 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 936
BamHI GGATCC 1 cut(s) 590
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 2 cut(s) 128, 936
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 4 cut(s) 257, 274, 392, 622
BccI CCATC 1 cut(s) 581
BciT130I CCWGG 2 cut(s) 396, 527
BciVI GTATCC 1 cut(s) 309
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 2 cut(s) 215, 726
BfmI CTRYAG 1 cut(s) 714
BfuI GTATCC 1 cut(s) 309
BglII AGATCT 1 cut(s) 967
BisI GCNGC 7 cut(s) 154, 246, 263, 307, 378, 381, 636
BlsI GCNGC 7 cut(s) 155, 247, 264, 308, 379, 382, 637
Bme1390I CCNGG 2 cut(s) 396, 527
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 398, 932, 933
BmiI GGNNCC 4 cut(s) 99, 310, 592, 934
BmrFI CCNGG 2 cut(s) 396, 527
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 2 cut(s) 428, 648
BmuI ACTGGG 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 184
BpmI CTGGAG 1 cut(s) 909
Bpu14I TTCGAA 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 498
BsaBI GATNNNNATC 1 cut(s) 418
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 138, 345, 526
BsaXI ACNNNNNCTCC 2 cut(s) 882, 912
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 645
Bse1I ACTGG 2 cut(s) 113, 128
Bse3DI GCAATG 2 cut(s) 11, 684
Bse8I GATNNNNATC 1 cut(s) 418
BseBI CCWGG 2 cut(s) 396, 527
BseDI CCNNGG 3 cut(s) 138, 345, 526
BseJI GATNNNNATC 1 cut(s) 418
BseLI CCNNNNNNNGG 2 cut(s) 318, 645
BseMI GCAATG 2 cut(s) 11, 684
BseMII CTCAG 3 cut(s) 66, 216, 975
BseNI ACTGG 2 cut(s) 113, 128
BseSI GKGCMC 1 cut(s) 936
BseXI GCAGC 4 cut(s) 257, 274, 392, 622
BseYI CCCAGC 2 cut(s) 248, 929
BsgI GTGCAG 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 1 cut(s) 934
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 712
BslFI GGGAC 1 cut(s) 931
BslI CCNNNNNNNGG 2 cut(s) 318, 645
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 931
BsnI GGCC 1 cut(s) 934
Bso31I GGTCTC 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 47
Bsp120I GGGCCC 1 cut(s) 932
Bsp1286I GDGCHC 3 cut(s) 128, 712, 936
Bsp143I GATC 5 cut(s) 132, 257, 590, 730, 967
BspACI CCGC 5 cut(s) 153, 165, 207, 306, 378
BspANI GGCC 1 cut(s) 934
BspCNI CTCAG 3 cut(s) 67, 217, 974
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 4 cut(s) 99, 310, 592, 934
BspPI GGATC 4 cut(s) 265, 585, 598, 738
BspT104I TTCGAA 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 11, 684
BsrI ACTGG 2 cut(s) 113, 128
BssECI CCNNGG 3 cut(s) 138, 345, 526
BssMI GATC 5 cut(s) 132, 257, 590, 730, 967
BssNAI GTATAC 1 cut(s) 480
BssT1I CCWWGG 1 cut(s) 345
Bst1107I GTATAC 1 cut(s) 480
Bst2UI CCWGG 2 cut(s) 396, 527
Bst4CI ACNGT 5 cut(s) 175, 190, 391, 496, 912
Bst6I CTCTTC 2 cut(s) 351, 849
BstAPI GCANNNNNTGC 1 cut(s) 683
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 741
BstDEI CTNAG 3 cut(s) 75, 225, 961
BstFNI CGCG 1 cut(s) 306
BstKTI GATC 5 cut(s) 135, 260, 593, 733, 970
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 5 cut(s) 132, 257, 590, 730, 967
BstMWI GCNNNNNNNGC 4 cut(s) 245, 425, 683, 716
BstNI CCWGG 2 cut(s) 396, 527
BstSCI CCNGG 2 cut(s) 394, 525
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 936
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 4 cut(s) 257, 274, 392, 622
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 3 cut(s) 590, 730, 967
BstYI RGATCY 3 cut(s) 590, 730, 967
BstZ17I GTATAC 1 cut(s) 480
BsuI GTATCC 1 cut(s) 309
BsuRI GGCC 1 cut(s) 934
BtsI GCAGTG 1 cut(s) 901
BtsIMutI CAGTG 3 cut(s) 120, 135, 901
Cac8I GCNNGC 1 cut(s) 741
Cfr13I GGNCC 3 cut(s) 398, 932, 933
CseI GACGC 1 cut(s) 732
Csp6I GTAC 2 cut(s) 98, 492
CviAII CATG 3 cut(s) 148, 374, 981
CviQI GTAC 2 cut(s) 98, 492
DdeI CTNAG 3 cut(s) 75, 225, 961
DpnI GATC 5 cut(s) 134, 259, 592, 732, 969
DpnII GATC 5 cut(s) 132, 257, 590, 730, 967
Eam1104I CTCTTC 2 cut(s) 351, 849
EarI CTCTTC 2 cut(s) 351, 849
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 2 cut(s) 128, 936
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 2 cut(s) 261, 423
EcoRII CCWGG 2 cut(s) 394, 525
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 2 cut(s) 128, 936
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 3 cut(s) 151, 377, 984
FalI AAGNNNNNCTT 4 cut(s) 431, 463, 846, 878
FaqI GGGAC 1 cut(s) 931
FatI CATG 3 cut(s) 147, 373, 980
FauI CCCGC 1 cut(s) 214
FbaI TGATCA 1 cut(s) 132
FblI GTMKAC 1 cut(s) 479
Fnu4HI GCNGC 7 cut(s) 154, 246, 263, 307, 378, 381, 636
FriOI GRGCYC 2 cut(s) 128, 936
Fsp4HI GCNGC 7 cut(s) 154, 246, 263, 307, 378, 381, 636
FspBI CTAG 2 cut(s) 215, 726
GluI GCNGC 7 cut(s) 154, 246, 263, 307, 378, 381, 636
GsaI CCCAGC 2 cut(s) 252, 933
GsuI CTGGAG 1 cut(s) 909
HaeIII GGCC 1 cut(s) 934
HgaI GACGC 1 cut(s) 732
Hin1II CATG 3 cut(s) 151, 377, 984
HincII GTYRAC 2 cut(s) 145, 409
HindII GTYRAC 2 cut(s) 145, 409
HinfI GANTC 6 cut(s) 30, 161, 509, 567, 722, 872
HphI GGTGA 2 cut(s) 127, 900
Hpy166II GTNNAC 4 cut(s) 118, 145, 409, 480
Hpy188I TCNGA 2 cut(s) 70, 365
Hpy188III TCNNGA 4 cut(s) 215, 278, 401, 869
Hpy8I GTNNAC 4 cut(s) 118, 145, 409, 480
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 467, 761, 801
HpyCH4III ACNGT 5 cut(s) 175, 190, 391, 496, 912
HpyCH4V TGCA 2 cut(s) 4, 110
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 425, 683, 716
HpyF3I CTNAG 3 cut(s) 75, 225, 961
Hsp92II CATG 3 cut(s) 151, 377, 984
KpnI GGTACC 1 cut(s) 101
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 5 cut(s) 132, 257, 590, 730, 967
LmnI GCTCC 2 cut(s) 314, 715
Lsp1109I GCAGC 4 cut(s) 257, 274, 392, 622
LweI GCATC 2 cut(s) 428, 648
MaeI CTAG 2 cut(s) 215, 726
MalI GATC 5 cut(s) 134, 259, 592, 732, 969
MboI GATC 5 cut(s) 132, 257, 590, 730, 967
MboII GAAGA 3 cut(s) 184, 368, 866
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 3 cut(s) 590, 730, 967
MhlI GDGCHC 3 cut(s) 128, 712, 936
MluCI AATT 6 cut(s) 43, 313, 597, 618, 797, 937
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 9 cut(s) 48, 112, 133, 193, 287, 834, 850, 884, 943
MseI TTAA 2 cut(s) 668, 1006
MslI CAYNNNNRTG 1 cut(s) 389
MspA1I CMGCKG 3 cut(s) 248, 265, 719
MspR9I CCNGG 2 cut(s) 396, 527
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 2 cut(s) 396, 527
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 4 cut(s) 245, 425, 683, 716
NdeII GATC 5 cut(s) 132, 257, 590, 730, 967
NlaIII CATG 3 cut(s) 151, 377, 984
NlaIV GGNNCC 4 cut(s) 99, 310, 592, 934
NspV TTCGAA 1 cut(s) 47
PfeI GAWTC 5 cut(s) 30, 509, 567, 722, 872
PflMI CCANNNNNTGG 1 cut(s) 645
PkrI GCNGC 7 cut(s) 155, 247, 264, 308, 379, 382, 637
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Psp6I CCWGG 2 cut(s) 394, 525
PspFI CCCAGC 2 cut(s) 248, 929
PspGI CCWGG 2 cut(s) 394, 525
PspN4I GGNNCC 4 cut(s) 99, 310, 592, 934
PspOMI GGGCCC 1 cut(s) 932
PspPI GGNCC 3 cut(s) 398, 932, 933
PsuI RGATCY 3 cut(s) 590, 730, 967
PvuII CAGCTG 3 cut(s) 248, 265, 719
RsaI GTAC 2 cut(s) 99, 493
RsaNI GTAC 2 cut(s) 98, 492
RseI CAYNNNNRTG 1 cut(s) 389
SaqAI TTAA 2 cut(s) 668, 1006
SatI GCNGC 7 cut(s) 154, 246, 263, 307, 378, 381, 636
Sau3AI GATC 5 cut(s) 132, 257, 590, 730, 967
Sau96I GGNCC 3 cut(s) 398, 932, 933
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 396, 527
SduI GDGCHC 3 cut(s) 128, 712, 936
SfaNI GCATC 2 cut(s) 428, 648
SfcI CTRYAG 1 cut(s) 714
SfuI TTCGAA 1 cut(s) 47
SinI GGWCC 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
Sse9I AATT 6 cut(s) 43, 313, 597, 618, 797, 937
SsiI CCGC 5 cut(s) 153, 165, 207, 306, 378
SspMI CTAG 2 cut(s) 215, 726
StyD4I CCNGG 2 cut(s) 394, 525
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 5 cut(s) 175, 190, 391, 496, 912
TaqI TCGA 2 cut(s) 47, 805
TaqII GACCGA 1 cut(s) 426
TasI AATT 6 cut(s) 43, 313, 597, 618, 797, 937
TatI WGTACW 1 cut(s) 491
TauI GCSGC 3 cut(s) 156, 309, 380
TfiI GAWTC 5 cut(s) 30, 509, 567, 722, 872
Tru1I TTAA 2 cut(s) 668, 1006
Tru9I TTAA 2 cut(s) 668, 1006
TscAI CASTG 3 cut(s) 120, 135, 901
TseI GCWGC 4 cut(s) 245, 262, 380, 635
TspGWI ACGGA 1 cut(s) 866
TspRI CASTG 3 cut(s) 120, 135, 901
Van91I CCANNNNNTGG 1 cut(s) 645
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 479
XspI CTAG 2 cut(s) 215, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.