AT3G44320

nitrilase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007736
Physical Location & Seq
Forward (+)
15993259 .. 15995811
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44320.1

Sequence Viewer

Length: 1041 bp
ATGTCTAGTACTGAAGAAATGTCATCAGTCAAAAACACAACTCAGGTTATTGGCGTTGACCCATCCTCCACCGTACGAGTTACCATCGTTCAATCTTCCACCGTCTATAATGATACCCCGGCCACTTTAGACAAGGCGGAGAAGTTTATTGTGGAGGCGGCGAGCAAAGGAGCGAAGTTGGTGTTGTTTCCCGAGGCGTTCATCGGCGGTTATCCTCGAGGTTTTAGGTTTGGTTTAGCGGTGGGAGTTCATAACGAAGAAGGGCGTGATGAGTTTCGTAACTACCATGCTTCTGCTATTAAAGTCCCTGGCCCTGAAGTGGAAAGATTGGCTGAGTTGGCCGGGAAAAATAATGTGCACTTGGTAATGGGGGCTATAGAGAAGGATGGTTATACACTCTATTGCACAGCTCTTTTCTTCAGTCCACAAGGTCAGTTTTTAGGTAAGCACCGTAAAGTCATGCCCACATCTTTGGAACGTTGCATATGGGGTCAAGGGGACGGATCAACCATCCCCGTTTACGACACTCCTATTGGCAAAATCGGTGCTGCTATTTGCTGGGAGAATAGGATGCCCCTCTACAGAACTGCATTGTACGCTAAAGGCATTGAGATTTATTGTGCACCTACTGCTGATTATTCGTTGGAATGGCAAGCATCGATGATTCACATTGCGGTAGAAGGTGGATGTTTCGTGTTGTCAGCGCACCAGTTCTGCAAGCGTAGAGAGTTCCCTGAACATCCTGATTACTTGTTTAATGACATAGTAGACACTAAAGAACATGATCCTACTGTCTCCGGAGGTGGAAGTGTCATTATTTCACCTTTGGGAAAGGTTCTCGCCGGACCAAACTATGAATCAGAGGGTCTCGTCACAGCTGATCTTGATCTTGGTGATATAGCAAGAGCCAAGTTATACTTCGATGTGGTTGGACATTACTCAAAGCCAGATATTTTTAACTTGACCGTAAATGAGCACCCGAAGAAACCGGTTACATTCATGACGAAGGTCGAGAAAGCAGAGGATGAATCAAACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000257 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009636 GO:0009683 GO:0009684 GO:0009850 GO:0009851 GO:0009970 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0010817 GO:0016020 GO:0016053 GO:0016143 GO:0016145 GO:0016787 GO:0016810 GO:0016815 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0018822 GO:0019438 GO:0019499 GO:0019748 GO:0019752 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0030054 GO:0031667 GO:0031668 GO:0031669 GO:0032787 GO:0033554 GO:0034641 GO:0034754 GO:0042221 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044248 GO:0044249 GO:0044271 GO:0044273 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046686 GO:0047427 GO:0047558 GO:0048046 GO:0050896 GO:0051410 GO:0051716 GO:0055044 GO:0065007 GO:0065008 GO:0071496 GO:0071704 GO:0071944 GO:0072330 GO:0080061 GO:0080109 GO:0098754 GO:1901135 GO:1901136 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1901698
KEGG Pathways
Metabolic & Signaling
ko00380 ko00460 ko00627 ko00643 ko00910 ko01100 ko01110 ko01120 map00380 map00460 map00627 map00643 map00910 map01100 map01110 map01120
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

346

Amino Acids

38.02

Weight (kDa)

5.62

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 26 - 304 3.6e-68 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 216
AccI GTMKAC 1 cut(s) 768
AccIII TCCGGA 1 cut(s) 797
AciI CCGC 5 cut(s) 137, 158, 207, 239, 674
AclI AACGTT 1 cut(s) 478
AclWI GGATC 2 cut(s) 511, 779
AcoI YGGCCR 2 cut(s) 120, 339
AcuI CTGAAG 3 cut(s) 33, 336, 403
AfaI GTAC 3 cut(s) 10, 75, 596
AfiI CCNNNNNNNGG 1 cut(s) 319
AgeI ACCGGT 1 cut(s) 988
AgsI TTSAA 1 cut(s) 92
AjnI CCWGG 1 cut(s) 307
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
AluBI AGCT 2 cut(s) 410, 878
AluI AGCT 2 cut(s) 410, 878
Alw21I GWGCWC 3 cut(s) 360, 625, 978
Alw26I GTCTC 2 cut(s) 799, 872
Alw44I GTGCAC 2 cut(s) 356, 621
AlwI GGATC 2 cut(s) 511, 779
Ama87I CYCGRG 2 cut(s) 191, 216
Aor13HI TCCGGA 1 cut(s) 797
AoxI GGCC 3 cut(s) 120, 310, 339
ApaLI GTGCAC 2 cut(s) 356, 621
ApeKI GCWGC 1 cut(s) 548
AsiGI ACCGGT 1 cut(s) 988
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 2 cut(s) 311, 845
AsuC2I CCSGG 2 cut(s) 119, 343
AsuHPI GGTGA 2 cut(s) 813, 905
AvaI CYCGRG 2 cut(s) 191, 216
AvaII GGWCC 1 cut(s) 845
BaeGI GKGCMC 2 cut(s) 360, 625
Bbv12I GWGCWC 3 cut(s) 360, 625, 978
BbvI GCAGC 1 cut(s) 535
BccI CCATC 4 cut(s) 70, 92, 380, 518
BciT130I CCWGG 1 cut(s) 309
BcnI CCSGG 2 cut(s) 119, 343
BcoDI GTCTC 2 cut(s) 799, 872
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 2 cut(s) 375, 580
BisI GCNGC 2 cut(s) 159, 549
BlsI GCNGC 2 cut(s) 160, 550
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 3 cut(s) 119, 309, 343
Bme18I GGWCC 1 cut(s) 845
BmeT110I CYCGRG 2 cut(s) 191, 216
BmgT120I GGNCC 2 cut(s) 311, 845
BmrFI CCNGG 3 cut(s) 119, 309, 343
BmsI GCATC 2 cut(s) 561, 665
BoxI GACNNNNGTC 1 cut(s) 1007
BpuMI CCSGG 2 cut(s) 119, 343
Bsa29I ATCGAT 1 cut(s) 659
BsaBI GATNNNNATC 1 cut(s) 885
BsaI GGTCTC 1 cut(s) 872
BsaJI CCNNGG 3 cut(s) 117, 192, 307
BsaWI WCCGGW 2 cut(s) 797, 988
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse118I RCCGGY 1 cut(s) 988
Bse1I ACTGG 1 cut(s) 709
Bse3DI GCAATG 1 cut(s) 669
Bse8I GATNNNNATC 1 cut(s) 885
BseAI TCCGGA 1 cut(s) 797
BseBI CCWGG 1 cut(s) 309
BseCI ATCGAT 1 cut(s) 659
BseDI CCNNGG 3 cut(s) 117, 192, 307
BseGI GGATG 7 cut(s) 62, 391, 510, 576, 692, 739, 1030
BseJI GATNNNNATC 1 cut(s) 885
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 2 cut(s) 56, 324
BseNI ACTGG 1 cut(s) 709
BseSI GKGCMC 2 cut(s) 360, 625
BseXI GCAGC 1 cut(s) 535
BseYI CCCAGC 1 cut(s) 558
BshFI GGCC 3 cut(s) 122, 312, 341
BshTI ACCGGT 1 cut(s) 988
BshVI ATCGAT 1 cut(s) 659
BsiHKAI GWGCWC 3 cut(s) 360, 625, 978
BsiHKCI CYCGRG 2 cut(s) 191, 216
BsiSI CCGG 5 cut(s) 119, 342, 798, 843, 989
BsiWI CGTACG 1 cut(s) 73
BslFI GGGAC 2 cut(s) 290, 512
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 2 cut(s) 799, 872
BsmFI GGGAC 2 cut(s) 290, 512
BsnI GGCC 3 cut(s) 122, 312, 341
Bso31I GGTCTC 1 cut(s) 872
BsoBI CYCGRG 2 cut(s) 191, 216
Bsp1286I GDGCHC 3 cut(s) 360, 625, 978
Bsp13I TCCGGA 1 cut(s) 797
Bsp143I GATC 4 cut(s) 503, 784, 880, 886
BspACI CCGC 5 cut(s) 137, 158, 207, 239, 674
BspANI GGCC 3 cut(s) 122, 312, 341
BspCNI CTCAG 2 cut(s) 55, 325
BspDI ATCGAT 1 cut(s) 659
BspEI TCCGGA 1 cut(s) 797
BspHI TCATGA 1 cut(s) 999
BspPI GGATC 2 cut(s) 511, 779
BspTNI GGTCTC 1 cut(s) 872
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 988
BsrI ACTGG 1 cut(s) 709
BssAI RCCGGY 1 cut(s) 988
BssECI CCNNGG 3 cut(s) 117, 192, 307
BssMI GATC 4 cut(s) 503, 784, 880, 886
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 5 cut(s) 73, 103, 452, 793, 967
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 3 cut(s) 163, 654, 719
BstDEI CTNAG 2 cut(s) 42, 333
BstF5I GGATG 7 cut(s) 62, 391, 510, 576, 692, 739, 1030
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 4 cut(s) 506, 787, 883, 889
BstMAI GTCTC 2 cut(s) 799, 872
BstMBI GATC 4 cut(s) 503, 784, 880, 886
BstMWI GCNNNNNNNGC 3 cut(s) 338, 596, 629
BstNI CCWGG 1 cut(s) 309
BstPAI GACNNNNGTC 1 cut(s) 1007
BstSCI CCNGG 3 cut(s) 117, 307, 341
BstSFI CTRYAG 2 cut(s) 375, 580
BstSLI GKGCMC 2 cut(s) 360, 625
BstV1I GCAGC 1 cut(s) 535
BstXI CCANNNNNNTGG 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 659
BsuRI GGCC 3 cut(s) 122, 312, 341
BsuTUI ATCGAT 1 cut(s) 659
BtsCI GGATG 7 cut(s) 62, 391, 510, 576, 692, 739, 1030
Cac8I GCNNGC 3 cut(s) 163, 654, 719
CciI TCATGA 1 cut(s) 999
CfoI GCGC 1 cut(s) 706
Cfr10I RCCGGY 1 cut(s) 988
Cfr13I GGNCC 2 cut(s) 311, 845
ClaI ATCGAT 1 cut(s) 659
Csp6I GTAC 3 cut(s) 9, 74, 595
CspAI ACCGGT 1 cut(s) 988
CviAII CATG 4 cut(s) 287, 460, 782, 1000
CviJI RGCY 9 cut(s) 122, 312, 332, 341, 374, 410, 878, 908, 946
CviKI_1 RGCY 9 cut(s) 122, 312, 332, 341, 374, 410, 878, 908, 946
CviQI GTAC 3 cut(s) 9, 74, 595
DdeI CTNAG 2 cut(s) 42, 333
DpnI GATC 4 cut(s) 505, 786, 882, 888
DpnII GATC 4 cut(s) 503, 784, 880, 886
EaeI YGGCCR 2 cut(s) 120, 339
EciI GGCGGA 1 cut(s) 152
Eco31I GGTCTC 1 cut(s) 872
Eco47I GGWCC 1 cut(s) 845
Eco57I CTGAAG 3 cut(s) 33, 336, 403
Eco88I CYCGRG 2 cut(s) 191, 216
EcoRII CCWGG 1 cut(s) 307
FaeI CATG 4 cut(s) 290, 463, 785, 1003
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 2 cut(s) 290, 512
FatI CATG 4 cut(s) 286, 459, 781, 999
FauNDI CATATG 1 cut(s) 485
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 2 cut(s) 159, 549
FokI GGATG 7 cut(s) 49, 398, 497, 583, 699, 726, 1037
Fsp4HI GCNGC 2 cut(s) 159, 549
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 705
GluI GCNGC 2 cut(s) 159, 549
GsaI CCCAGC 1 cut(s) 562
HaeIII GGCC 3 cut(s) 122, 312, 341
HapII CCGG 5 cut(s) 119, 342, 798, 843, 989
HhaI GCGC 1 cut(s) 706
Hin1II CATG 4 cut(s) 290, 463, 785, 1003
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 3 cut(s) 664, 857, 1028
HpaII CCGG 5 cut(s) 119, 342, 798, 843, 989
HphI GGTGA 2 cut(s) 813, 905
Hpy166II GTNNAC 6 cut(s) 58, 358, 425, 520, 623, 769
Hpy188I TCNGA 1 cut(s) 862
Hpy188III TCNNGA 6 cut(s) 191, 743, 798, 884, 1000, 1012
Hpy8I GTNNAC 6 cut(s) 58, 358, 425, 520, 623, 769
HpyAV CCTTC 4 cut(s) 254, 376, 674, 1000
HpyCH4III ACNGT 5 cut(s) 73, 103, 452, 793, 967
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 6 cut(s) 358, 405, 483, 590, 623, 717
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 596, 629
HpyF3I CTNAG 2 cut(s) 42, 333
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 4 cut(s) 290, 463, 785, 1003
HspAI GCGC 1 cut(s) 704
Kpn2I TCCGGA 1 cut(s) 797
Kzo9I GATC 4 cut(s) 503, 784, 880, 886
LmnI GCTCC 1 cut(s) 170
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 2 cut(s) 561, 665
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 4 cut(s) 79, 278, 871, 991
MalI GATC 4 cut(s) 505, 786, 882, 888
MboI GATC 4 cut(s) 503, 784, 880, 886
MboII GAAGA 5 cut(s) 26, 87, 269, 409, 994
MhlI GDGCHC 3 cut(s) 360, 625, 978
MmeI TCCRAC 2 cut(s) 624, 910
MnlI CCTC 9 cut(s) 76, 148, 187, 212, 225, 587, 794, 856, 1015
MroI TCCGGA 1 cut(s) 797
MseI TTAA 3 cut(s) 300, 756, 957
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 5 cut(s) 119, 342, 798, 843, 989
MspR9I CCNGG 3 cut(s) 119, 309, 343
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 3 cut(s) 338, 596, 629
NciI CCSGG 2 cut(s) 119, 343
NdeI CATATG 1 cut(s) 485
NdeII GATC 4 cut(s) 503, 784, 880, 886
NlaIII CATG 4 cut(s) 290, 463, 785, 1003
NmuCI GTSAC 1 cut(s) 871
PaeR7I CTCGAG 1 cut(s) 216
PagI TCATGA 1 cut(s) 999
PfeI GAWTC 3 cut(s) 664, 857, 1028
Pfl23II CGTACG 1 cut(s) 73
PinAI ACCGGT 1 cut(s) 988
PkrI GCNGC 2 cut(s) 160, 550
PshAI GACNNNNGTC 1 cut(s) 1007
Psp1406I AACGTT 1 cut(s) 478
Psp6I CCWGG 1 cut(s) 307
PspFI CCCAGC 1 cut(s) 558
PspGI CCWGG 1 cut(s) 307
PspLI CGTACG 1 cut(s) 73
PspPI GGNCC 2 cut(s) 311, 845
PspXI VCTCGAGB 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 3 cut(s) 10, 75, 596
RsaNI GTAC 3 cut(s) 9, 74, 595
SaqAI TTAA 3 cut(s) 300, 756, 957
SatI GCNGC 2 cut(s) 159, 549
Sau3AI GATC 4 cut(s) 503, 784, 880, 886
Sau96I GGNCC 2 cut(s) 311, 845
ScaI AGTACT 1 cut(s) 10
ScrFI CCNGG 3 cut(s) 119, 309, 343
SduI GDGCHC 3 cut(s) 360, 625, 978
SfaNI GCATC 2 cut(s) 561, 665
SfcI CTRYAG 2 cut(s) 375, 580
Sfr274I CTCGAG 1 cut(s) 216
SinI GGWCC 1 cut(s) 845
SlaI CTCGAG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
SsiI CCGC 5 cut(s) 137, 158, 207, 239, 674
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 3 cut(s) 117, 307, 341
TaaI ACNGT 5 cut(s) 73, 103, 452, 793, 967
TaiI ACGT 1 cut(s) 481
TaqI TCGA 4 cut(s) 217, 659, 921, 1011
TatI WGTACW 1 cut(s) 8
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 3 cut(s) 664, 857, 1028
Tru1I TTAA 3 cut(s) 300, 756, 957
Tru9I TTAA 3 cut(s) 300, 756, 957
TseFI GTSAC 1 cut(s) 871
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 1 cut(s) 871
TspDTI ATGAA 5 cut(s) 190, 239, 870, 988, 1041
TspGWI ACGGA 1 cut(s) 516
VneI GTGCAC 2 cut(s) 356, 621
VpaK11BI GGWCC 1 cut(s) 845
XhoI CTCGAG 1 cut(s) 216
XmiI GTMKAC 1 cut(s) 768
XspI CTAG 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.