AT3G44300

nitrilase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007736
Physical Location & Seq
Forward (+)
15983199 .. 15985535
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44300.1

Sequence Viewer

Length: 1020 bp
ATGTCAACTTCAGAAAACACTCCGTTTAATGGCGTTGCCTCATCCACCATTGTTCGAGCTACCATTGTCCAAGCCTCCACCGTCTACAACGATACTCCCGCCACTCTAGAAAAGGCGAACAAGTTTATTGTGGAGGCTGCAAGCAAGGGATCGGAGCTGGTTGTGTTCCCGGAGGCGTTTATCGGTGGTTATCCTCGAGGTTTTAGGTTTGGTTTAGGGGTGGGAGTTCATAACGAAGAAGGGCGTGATGAGTTCCGCAAGTACCATGCTTCTGCTATTAAAGTTCCTGGCCCTGAAGTAGAAAAGTTGGCGGAGTTGGCCGGGAAGAACAATGTGTACTTGGTAATGGGAGCGATAGAGAAGGATGGGTATACACTCTATTGCACAGCACTTTTCTTCAGTCCACAAGGTCAGTTCTTGGGTAAGCACCGTAAACTCATGCCCACAAGTTTGGAACGTTGCATTTGGGGTCAAGGAGACGGATCAACCATCCCCGTTTACGACACTCCGATTGGAAAACTCGGTGCTGCTATTTGCTGGGAGAATAGGATGCCCCTCTACAGAACTGCTTTGTACGCCAAAGGCATTGAGCTTTATTGTGCACCTACTGCTGATGGTTCGAAAGAATGGCAATCGTCGATGCTTCACATTGCGATCGAAGGTGGATGTTTCGTATTGTCGGCTTGCCAGTTCTGCCTTCGTAAAGATTTCCCTGATCATCCTGACTACTTGTTTACCGATTGGTACGACGACAAAGAGCCTGACTCTATTGTTTCCCAAGGTGGAAGTGTTATTATTTCACCTTTGGGACAGGTTCTTGCGGGACCAAACTTTGAATCAGAGGGTCTCATCACAGCTGATCTTGATCTTGGTGATGTAGCAAGAGCTAAGTTGTACTTCGATTCGGTTGGACATTACTCGAGACCAGATGTTTTACACTTGACCGTAAATGAGCACCCGAAGAAACCGGTCACATTCATTTCGAAGGTGGAGAAAGCGGAAGATGACTCAAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000257 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009636 GO:0009683 GO:0009684 GO:0009850 GO:0009851 GO:0009970 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0010817 GO:0016020 GO:0016053 GO:0016143 GO:0016145 GO:0016787 GO:0016810 GO:0016815 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0018822 GO:0019438 GO:0019499 GO:0019748 GO:0019752 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0030054 GO:0031667 GO:0031668 GO:0031669 GO:0032787 GO:0033554 GO:0034641 GO:0034754 GO:0042221 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044248 GO:0044249 GO:0044271 GO:0044273 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046686 GO:0047427 GO:0047558 GO:0048046 GO:0050896 GO:0051410 GO:0051716 GO:0055044 GO:0065007 GO:0065008 GO:0071496 GO:0071704 GO:0071944 GO:0072330 GO:0080061 GO:0080109 GO:0098754 GO:1901135 GO:1901136 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1901698
KEGG Pathways
Metabolic & Signaling
ko00380 ko00460 ko00627 ko00643 ko00910 ko01100 ko01110 ko01120 map00380 map00460 map00627 map00643 map00910 map01100 map01110 map01120
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

339

Amino Acids

37.15

Weight (kDa)

5.35

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 19 - 297 4e-69 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 195
AccI GTMKAC 2 cut(s) 84, 371
AciI CCGC 5 cut(s) 99, 256, 311, 821, 998
AclI AACGTT 1 cut(s) 457
AclWI GGATC 2 cut(s) 157, 490
AcoI YGGCCR 1 cut(s) 318
AcuI CTGAAG 2 cut(s) 315, 382
AfaI GTAC 5 cut(s) 263, 338, 575, 746, 896
AfiI CCNNNNNNNGG 1 cut(s) 29
AgeI ACCGGT 1 cut(s) 967
AgsI TTSAA 1 cut(s) 836
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 5 cut(s) 59, 157, 592, 857, 887
AluI AGCT 5 cut(s) 59, 157, 592, 857, 887
Alw21I GWGCWC 2 cut(s) 604, 957
Alw26I GTCTC 3 cut(s) 471, 851, 916
Alw44I GTGCAC 1 cut(s) 600
AlwI GGATC 2 cut(s) 157, 490
Ama87I CYCGRG 2 cut(s) 195, 919
AoxI GGCC 2 cut(s) 289, 318
ApaLI GTGCAC 1 cut(s) 600
ApeKI GCWGC 2 cut(s) 137, 527
AsiGI ACCGGT 1 cut(s) 967
AspS9I GGNCC 2 cut(s) 290, 824
AsuC2I CCSGG 2 cut(s) 170, 322
AsuHPI GGTGA 2 cut(s) 792, 884
AsuII TTCGAA 2 cut(s) 620, 983
AvaI CYCGRG 2 cut(s) 195, 919
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 1 cut(s) 604
Bbv12I GWGCWC 2 cut(s) 604, 957
BbvI GCAGC 2 cut(s) 124, 514
BccI CCATC 3 cut(s) 359, 497, 608
BciT130I CCWGG 1 cut(s) 288
BclI TGATCA 1 cut(s) 715
BcnI CCSGG 2 cut(s) 170, 322
BcoDI GTCTC 3 cut(s) 471, 851, 916
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 559
BisI GCNGC 2 cut(s) 138, 528
BlsI GCNGC 2 cut(s) 139, 529
Bme1390I CCNGG 3 cut(s) 170, 288, 322
Bme18I GGWCC 1 cut(s) 824
BmeT110I CYCGRG 2 cut(s) 195, 919
BmgT120I GGNCC 2 cut(s) 290, 824
BmiI GGNNCC 1 cut(s) 825
BmrFI CCNGG 3 cut(s) 170, 288, 322
BmsI GCATC 2 cut(s) 540, 630
BplI GAGNNNNNCTC 2 cut(s) 749, 781
Bpu14I TTCGAA 2 cut(s) 620, 983
BpuMI CCSGG 2 cut(s) 170, 322
BsaBI GATNNNNATC 1 cut(s) 864
BsaI GGTCTC 2 cut(s) 851, 916
BsaJI CCNNGG 1 cut(s) 778
BsaWI WCCGGW 1 cut(s) 967
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 967
Bse1I ACTGG 1 cut(s) 688
Bse3DI GCAATG 1 cut(s) 648
Bse8I GATNNNNATC 1 cut(s) 864
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 778
BseGI GGATG 6 cut(s) 41, 370, 489, 555, 671, 718
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 648
BseNI ACTGG 1 cut(s) 688
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 2 cut(s) 124, 514
BseYI CCCAGC 1 cut(s) 537
Bsh1285I CGRYCG 1 cut(s) 657
BshFI GGCC 2 cut(s) 291, 320
BshTI ACCGGT 1 cut(s) 967
BsiEI CGRYCG 1 cut(s) 657
BsiHKAI GWGCWC 2 cut(s) 604, 957
BsiHKCI CYCGRG 2 cut(s) 195, 919
BsiSI CCGG 3 cut(s) 170, 321, 968
BslFI GGGAC 2 cut(s) 822, 837
BslI CCNNNNNNNGG 1 cut(s) 29
BsmAI GTCTC 3 cut(s) 471, 851, 916
BsmBI CGTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 822, 837
BsnI GGCC 2 cut(s) 291, 320
Bso31I GGTCTC 2 cut(s) 851, 916
BsoBI CYCGRG 2 cut(s) 195, 919
Bsp119I TTCGAA 2 cut(s) 620, 983
Bsp1286I GDGCHC 2 cut(s) 604, 957
Bsp143I GATC 6 cut(s) 149, 482, 654, 715, 859, 865
BspACI CCGC 5 cut(s) 99, 256, 311, 821, 998
BspANI GGCC 2 cut(s) 291, 320
BspLI GGNNCC 1 cut(s) 825
BspPI GGATC 2 cut(s) 157, 490
BspT104I TTCGAA 2 cut(s) 620, 983
BspTNI GGTCTC 2 cut(s) 851, 916
BsrDI GCAATG 1 cut(s) 648
BsrFI RCCGGY 1 cut(s) 967
BsrI ACTGG 1 cut(s) 688
BssAI RCCGGY 1 cut(s) 967
BssECI CCNNGG 1 cut(s) 778
BssMI GATC 6 cut(s) 149, 482, 654, 715, 859, 865
BssNAI GTATAC 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 778
Bst1107I GTATAC 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 3 cut(s) 82, 431, 946
BstAPI GCANNNNNTGC 1 cut(s) 608
BstBI TTCGAA 2 cut(s) 620, 983
BstC8I GCNNGC 2 cut(s) 142, 685
BstDEI CTNAG 1 cut(s) 888
BstF5I GGATG 6 cut(s) 41, 370, 489, 555, 671, 718
BstKTI GATC 6 cut(s) 152, 485, 657, 718, 862, 868
BstMAI GTCTC 3 cut(s) 471, 851, 916
BstMBI GATC 6 cut(s) 149, 482, 654, 715, 859, 865
BstMCI CGRYCG 1 cut(s) 657
BstMWI GCNNNNNNNGC 4 cut(s) 317, 575, 608, 693
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 168, 286, 320
BstSFI CTRYAG 1 cut(s) 559
BstSLI GKGCMC 1 cut(s) 604
BstV1I GCAGC 2 cut(s) 124, 514
BstXI CCANNNNNNTGG 1 cut(s) 451
BstZ17I GTATAC 1 cut(s) 372
BsuRI GGCC 2 cut(s) 291, 320
BtsCI GGATG 6 cut(s) 41, 370, 489, 555, 671, 718
Cac8I GCNNGC 2 cut(s) 142, 685
Cfr10I RCCGGY 1 cut(s) 967
Cfr13I GGNCC 2 cut(s) 290, 824
Csp6I GTAC 5 cut(s) 262, 337, 574, 745, 895
CspAI ACCGGT 1 cut(s) 967
CviAII CATG 2 cut(s) 266, 439
CviQI GTAC 5 cut(s) 262, 337, 574, 745, 895
DdeI CTNAG 1 cut(s) 888
DpnI GATC 6 cut(s) 151, 484, 656, 717, 861, 867
DpnII GATC 6 cut(s) 149, 482, 654, 715, 859, 865
EaeI YGGCCR 1 cut(s) 318
EciI GGCGGA 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 778
Eco31I GGTCTC 2 cut(s) 851, 916
Eco47I GGWCC 1 cut(s) 824
Eco57I CTGAAG 2 cut(s) 315, 382
Eco88I CYCGRG 2 cut(s) 195, 919
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 778
ErhI CCWWGG 1 cut(s) 778
Esp3I CGTCTC 1 cut(s) 471
FaeI CATG 2 cut(s) 269, 442
FaiI YATR 4 cut(s) 231, 267, 372, 440
FalI AAGNNNNNCTT 2 cut(s) 881, 913
FaqI GGGAC 2 cut(s) 822, 837
FatI CATG 2 cut(s) 265, 438
FauI CCCGC 2 cut(s) 106, 814
FbaI TGATCA 1 cut(s) 715
FblI GTMKAC 2 cut(s) 84, 371
Fnu4HI GCNGC 2 cut(s) 138, 528
FokI GGATG 6 cut(s) 28, 377, 476, 562, 678, 705
Fsp4HI GCNGC 2 cut(s) 138, 528
FspBI CTAG 1 cut(s) 107
GluI GCNGC 2 cut(s) 138, 528
GsaI CCCAGC 1 cut(s) 541
HaeIII GGCC 2 cut(s) 291, 320
HapII CCGG 3 cut(s) 170, 321, 968
Hin1II CATG 2 cut(s) 269, 442
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 764, 836, 902, 1007
HpaII CCGG 3 cut(s) 170, 321, 968
HphI GGTGA 2 cut(s) 792, 884
Hpy166II GTNNAC 9 cut(s) 6, 85, 337, 372, 404, 434, 499, 602, 735
Hpy188I TCNGA 4 cut(s) 13, 154, 510, 841
Hpy188III TCNNGA 4 cut(s) 107, 722, 863, 921
Hpy8I GTNNAC 9 cut(s) 6, 85, 337, 372, 404, 434, 499, 602, 735
Hpy99I CGWCG 2 cut(s) 640, 752
HpyAV CCTTC 5 cut(s) 233, 355, 653, 707, 979
HpyCH4III ACNGT 3 cut(s) 82, 431, 946
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 4 cut(s) 140, 384, 462, 602
HpyF10VI GCNNNNNNNGC 4 cut(s) 317, 575, 608, 693
HpyF3I CTNAG 1 cut(s) 888
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 2 cut(s) 269, 442
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 6 cut(s) 149, 482, 654, 715, 859, 865
LmnI GCTCC 2 cut(s) 154, 350
Lsp1109I GCAGC 2 cut(s) 124, 514
LweI GCATC 2 cut(s) 540, 630
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 1 cut(s) 970
MalI GATC 6 cut(s) 151, 484, 656, 717, 861, 867
MboI GATC 6 cut(s) 149, 482, 654, 715, 859, 865
MboII GAAGA 5 cut(s) 248, 337, 388, 973, 1013
MhlI GDGCHC 2 cut(s) 604, 957
MlyI GAGTC 2 cut(s) 758, 1001
MmeI TCCRAC 1 cut(s) 889
MnlI CCTC 8 cut(s) 49, 85, 127, 166, 191, 204, 566, 835
MseI TTAA 2 cut(s) 27, 279
MspA1I CMGCKG 1 cut(s) 857
MspI CCGG 3 cut(s) 170, 321, 968
MspR9I CCNGG 3 cut(s) 170, 288, 322
MvaI CCWGG 1 cut(s) 288
MwoI GCNNNNNNNGC 4 cut(s) 317, 575, 608, 693
NciI CCSGG 2 cut(s) 170, 322
NdeII GATC 6 cut(s) 149, 482, 654, 715, 859, 865
NlaIII CATG 2 cut(s) 269, 442
NlaIV GGNNCC 1 cut(s) 825
NmuCI GTSAC 1 cut(s) 970
NspV TTCGAA 2 cut(s) 620, 983
PaeR7I CTCGAG 2 cut(s) 195, 919
PfeI GAWTC 2 cut(s) 836, 902
PfoI TCCNGGA 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 967
PkrI GCNGC 2 cut(s) 139, 529
Ple19I CGATCG 1 cut(s) 657
PleI GAGTC 2 cut(s) 758, 1001
PpsI GAGTC 2 cut(s) 758, 1001
Psp1406I AACGTT 1 cut(s) 457
Psp6I CCWGG 1 cut(s) 286
PspFI CCCAGC 1 cut(s) 537
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 825
PspPI GGNCC 2 cut(s) 290, 824
PspXI VCTCGAGB 1 cut(s) 195
PsrI GAACNNNNNNTAC 2 cut(s) 320, 352
PvuI CGATCG 1 cut(s) 657
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 5 cut(s) 263, 338, 575, 746, 896
RsaNI GTAC 5 cut(s) 262, 337, 574, 745, 895
SaqAI TTAA 2 cut(s) 27, 279
SatI GCNGC 2 cut(s) 138, 528
Sau3AI GATC 6 cut(s) 149, 482, 654, 715, 859, 865
Sau96I GGNCC 2 cut(s) 290, 824
SchI GAGTC 2 cut(s) 758, 1001
ScrFI CCNGG 3 cut(s) 170, 288, 322
SduI GDGCHC 2 cut(s) 604, 957
SfaNI GCATC 2 cut(s) 540, 630
SfcI CTRYAG 1 cut(s) 559
Sfr274I CTCGAG 2 cut(s) 195, 919
SfuI TTCGAA 2 cut(s) 620, 983
SinI GGWCC 1 cut(s) 824
SlaI CTCGAG 2 cut(s) 195, 919
SmlI CTYRAG 2 cut(s) 195, 919
SmoI CTYRAG 2 cut(s) 195, 919
SsiI CCGC 5 cut(s) 99, 256, 311, 821, 998
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 3 cut(s) 168, 286, 320
StyI CCWWGG 1 cut(s) 778
TaaI ACNGT 3 cut(s) 82, 431, 946
TaiI ACGT 1 cut(s) 460
TaqI TCGA 8 cut(s) 55, 196, 620, 638, 657, 900, 920, 983
TatI WGTACW 2 cut(s) 336, 894
TfiI GAWTC 2 cut(s) 836, 902
Tru1I TTAA 2 cut(s) 27, 279
Tru9I TTAA 2 cut(s) 27, 279
TseFI GTSAC 1 cut(s) 970
TseI GCWGC 2 cut(s) 137, 527
Tsp45I GTSAC 1 cut(s) 970
TspDTI ATGAA 2 cut(s) 218, 967
TspGWI ACGGA 2 cut(s) 12, 495
VneI GTGCAC 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 824
XbaI TCTAGA 1 cut(s) 106
XhoI CTCGAG 2 cut(s) 195, 919
XmiI GTMKAC 2 cut(s) 84, 371
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.