Rorug05G0233300

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007736
Physical Location & Seq
Reverse (-)
24332677 .. 24333581
905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0233300.1

Sequence Viewer

Length: 714 bp
ATGGAACCACGAACTCTGCAACTGATGGAGCTACAAACCCAGCAACAAATCGAGGATGGAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACAGATTTCTGGCGAAGCCGTGCCAGGTGGGTTCCTACTCCAGATCAAATAAGGATCCTCAAGGATCTTTACTACGACAAGGGAGTTAAGCGCCCAACTACAGAGCATATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGACATGTTGAGGGCAAGAACATTTATTTTTGGTTCCAGAATGTCAGGGCTCGAGAGAAGCAGATGAAGAGGTGCAATCAGGCTGCTCAAGTGCCCATGGGAACTAGTTCTCCTGGTACTGGTGGATCCATTGATCTCAATTTTGGGCCAGCTGGTTCTACTGGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGATGATGGTAGATCCATTAATCTCAACTTTGGTTCCACTGATTCTACTGGTAATGAAGGATTTCTTGATTTAAATTTTGTTTCATATTCTTCCTCACACTTCAACACTAATACCAGTACAACTCTTTTGGCACAACAGGAGGACAAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGATTGAAACCCTTCCATTGTTCCCCATGCATGGTGAGGACATCTTGGGCATCATGA

Protein Analysis

237

Amino Acids

26.18

Weight (kDa)

5.86

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 38 - 99 1.1e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 437
AcsI RAATTY 1 cut(s) 550
AfaI GTAC 2 cut(s) 352, 595
AfiI CCNNNNNNNGG 3 cut(s) 353, 437, 688
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 2 cut(s) 580, 664
AhlI ACTAGT 2 cut(s) 338, 467
AjnI CCWGG 3 cut(s) 116, 346, 653
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AloI GAACNNNNNNTCC 2 cut(s) 328, 360
AluBI AGCT 4 cut(s) 31, 223, 386, 434
AluI AGCT 4 cut(s) 31, 223, 386, 434
Alw26I GTCTC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 2 cut(s) 380, 428
ApeKI GCWGC 2 cut(s) 74, 317
ApoI RAATTY 1 cut(s) 550
ArsI GACNNNNNNTTYG 4 cut(s) 407, 439, 440, 472
AseI ATTAAT 1 cut(s) 495
Asp700I GAANNNNTTC 3 cut(s) 340, 537, 668
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 3 cut(s) 380, 428, 462
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 643, 703
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 330
BamHI GGATCC 4 cut(s) 147, 359, 407, 440
BanII GRGCYC 1 cut(s) 286
BauI CACGAG 1 cut(s) 206
BbvI GCAGC 2 cut(s) 86, 304
BccI CCATC 4 cut(s) 19, 50, 419, 476
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 3 cut(s) 118, 348, 655
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 221
BcuI ACTAGT 2 cut(s) 338, 467
BfaI CTAG 2 cut(s) 339, 468
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 187
BglII AGATCT 1 cut(s) 210
BisI GCNGC 2 cut(s) 75, 318
BlsI GCNGC 2 cut(s) 76, 319
Bme1390I CCNGG 4 cut(s) 79, 118, 348, 655
Bme18I GGWCC 1 cut(s) 462
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 380, 428, 462
BmiI GGNNCC 9 cut(s) 6, 126, 149, 269, 361, 409, 442, 463, 511
BmrFI CCNGG 4 cut(s) 79, 118, 348, 655
BpmI CTGGAG 2 cut(s) 117, 203
BpuEI CTTGAG 2 cut(s) 137, 306
BpuMI CCSGG 1 cut(s) 79
BsaJI CCNNGG 1 cut(s) 330
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 437, 688
Bse1I ACTGG 5 cut(s) 229, 358, 400, 529, 591
BseBI CCWGG 3 cut(s) 118, 348, 655
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 2 cut(s) 61, 625
BseLI CCNNNNNNNGG 3 cut(s) 353, 437, 688
BseNI ACTGG 5 cut(s) 229, 358, 400, 529, 591
BseRI GAGGAG 2 cut(s) 656, 659
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 2 cut(s) 86, 304
BseYI CCCAGC 1 cut(s) 39
BshFI GGCC 2 cut(s) 382, 430
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 1 cut(s) 78
BslI CCNNNNNNNGG 3 cut(s) 353, 437, 688
BsmAI GTCTC 1 cut(s) 221
BsnI GGCC 2 cut(s) 382, 430
BsoBI CYCGRG 1 cut(s) 285
Bsp1286I GDGCHC 2 cut(s) 286, 330
Bsp19I CCATGG 1 cut(s) 330
BspANI GGCC 2 cut(s) 382, 430
BspHI TCATGA 1 cut(s) 710
BspLI GGNNCC 9 cut(s) 6, 126, 149, 269, 361, 409, 442, 463, 511
BsrI ACTGG 5 cut(s) 229, 358, 400, 529, 591
BssECI CCNNGG 1 cut(s) 330
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 330
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 3 cut(s) 118, 348, 655
Bst6I CTCTTC 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 384, 432
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 2 cut(s) 61, 625
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 1 cut(s) 186
BstMAI GTCTC 1 cut(s) 221
BstNI CCWGG 3 cut(s) 118, 348, 655
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 4 cut(s) 77, 116, 346, 653
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 86, 304
BstX2I RGATCY 7 cut(s) 147, 157, 210, 359, 407, 440, 488
BstYI RGATCY 7 cut(s) 147, 157, 210, 359, 407, 440, 488
BsuRI GGCC 2 cut(s) 382, 430
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 61, 625
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 384, 432
CciI TCATGA 1 cut(s) 710
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 3 cut(s) 380, 428, 462
Csp6I GTAC 2 cut(s) 351, 594
CviAII CATG 5 cut(s) 239, 331, 684, 688, 711
CviQI GTAC 2 cut(s) 351, 594
DraI TTTAAA 1 cut(s) 549
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 285
EcoRII CCWGG 3 cut(s) 116, 346, 653
EcoT14I CCWWGG 1 cut(s) 330
EcoT22I ATGCAT 1 cut(s) 689
EcoT38I GRGCYC 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 5 cut(s) 242, 334, 687, 691, 714
FaiI YATR 8 cut(s) 201, 234, 240, 332, 562, 685, 689, 712
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FatI CATG 5 cut(s) 238, 330, 683, 687, 710
Fnu4HI GCNGC 2 cut(s) 75, 318
FokI GGATG 2 cut(s) 68, 612
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 2 cut(s) 75, 318
FspBI CTAG 2 cut(s) 339, 468
GlaI GCGC 1 cut(s) 185
GluI GCNGC 2 cut(s) 75, 318
GsaI CCCAGC 1 cut(s) 43
GsuI CTGGAG 2 cut(s) 117, 203
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 2 cut(s) 382, 430
HapII CCGG 1 cut(s) 78
HhaI GCGC 1 cut(s) 186
Hin1II CATG 5 cut(s) 242, 334, 687, 691, 714
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 1 cut(s) 518
HpaII CCGG 1 cut(s) 78
HphI GGTGA 2 cut(s) 643, 703
Hpy166II GTNNAC 1 cut(s) 465
Hpy188III TCNNGA 6 cut(s) 134, 206, 271, 287, 542, 711
Hpy8I GTNNAC 1 cut(s) 465
HpyAV CCTTC 2 cut(s) 527, 679
HpyCH4V TGCA 4 cut(s) 19, 309, 633, 687
Hsp92II CATG 5 cut(s) 242, 334, 687, 691, 714
HspAI GCGC 1 cut(s) 184
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 2 cut(s) 86, 304
MaeI CTAG 2 cut(s) 339, 468
MboII GAAGA 2 cut(s) 313, 558
MflI RGATCY 7 cut(s) 147, 157, 210, 359, 407, 440, 488
MhlI GDGCHC 2 cut(s) 286, 330
MluCI AATT 4 cut(s) 373, 421, 454, 550
Mph1103I ATGCAT 1 cut(s) 689
MroXI GAANNNNTTC 3 cut(s) 340, 537, 668
MseI TTAA 3 cut(s) 180, 495, 548
MspA1I CMGCKG 3 cut(s) 223, 386, 434
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 4 cut(s) 79, 118, 348, 655
MvaI CCWGG 3 cut(s) 118, 348, 655
NciI CCSGG 1 cut(s) 79
NcoI CCATGG 1 cut(s) 330
NlaIII CATG 5 cut(s) 242, 334, 687, 691, 714
NlaIV GGNNCC 9 cut(s) 6, 126, 149, 269, 361, 409, 442, 463, 511
NsiI ATGCAT 1 cut(s) 689
NspI RCATGY 1 cut(s) 242
PaeR7I CTCGAG 1 cut(s) 285
PagI TCATGA 1 cut(s) 710
PciI ACATGT 1 cut(s) 238
PdmI GAANNNNTTC 3 cut(s) 340, 537, 668
PfeI GAWTC 1 cut(s) 518
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 2 cut(s) 76, 319
PscI ACATGT 1 cut(s) 238
PshBI ATTAAT 1 cut(s) 495
Psp6I CCWGG 3 cut(s) 116, 346, 653
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 3 cut(s) 116, 346, 653
PspN4I GGNNCC 9 cut(s) 6, 126, 149, 269, 361, 409, 442, 463, 511
PspPI GGNCC 3 cut(s) 380, 428, 462
PsuI RGATCY 7 cut(s) 147, 157, 210, 359, 407, 440, 488
PvuII CAGCTG 3 cut(s) 223, 386, 434
RsaI GTAC 2 cut(s) 352, 595
RsaNI GTAC 2 cut(s) 351, 594
SaqAI TTAA 3 cut(s) 180, 495, 548
SatI GCNGC 2 cut(s) 75, 318
Sau96I GGNCC 3 cut(s) 380, 428, 462
ScrFI CCNGG 4 cut(s) 79, 118, 348, 655
SduI GDGCHC 2 cut(s) 286, 330
SetI ASST 6 cut(s) 33, 122, 225, 308, 388, 436
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 285
SinI GGWCC 1 cut(s) 462
SlaI CTCGAG 1 cut(s) 285
SmiI ATTTAAAT 1 cut(s) 549
SmlI CTYRAG 3 cut(s) 152, 285, 321
SmoI CTYRAG 3 cut(s) 152, 285, 321
SpeI ACTAGT 2 cut(s) 338, 467
Sse9I AATT 4 cut(s) 373, 421, 454, 550
SspMI CTAG 2 cut(s) 339, 468
StyD4I CCNGG 4 cut(s) 77, 116, 346, 653
StyI CCWWGG 1 cut(s) 330
SwaI ATTTAAAT 1 cut(s) 549
TaqI TCGA 3 cut(s) 51, 286, 414
TasI AATT 4 cut(s) 373, 421, 454, 550
TatI WGTACW 1 cut(s) 593
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 3 cut(s) 180, 495, 548
Tru9I TTAA 3 cut(s) 180, 495, 548
TscAI CASTG 1 cut(s) 520
TseI GCWGC 2 cut(s) 74, 317
TspDTI ATGAA 3 cut(s) 314, 546, 549
TspRI CASTG 1 cut(s) 520
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 462
VspI ATTAAT 1 cut(s) 495
XapI RAATTY 1 cut(s) 550
XceI RCATGY 1 cut(s) 242
XhoI CTCGAG 1 cut(s) 285
XmnI GAANNNNTTC 3 cut(s) 340, 537, 668
XspI CTAG 2 cut(s) 339, 468
Zsp2I ATGCAT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.