AT5G22300

nitrilase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007736
Physical Location & Seq
Forward (+)
7379187 .. 7382025
2839 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22300.2

Sequence Viewer

Length: 1068 bp
ATGTCCATGCAACAAGAAACGTCTCACATGACGGCGGCTCCACAGACCAACGGCCATCAAATCTTCCCTGAGATTGACATGTCCGCCGGCGATTCCTCCTCCATCGTACGAGCTACAGTCGTCCAAGCCTCTACAGTCTTCTACGATACTCCCGCCACGCTAGATAAGGCAGAGAGACTGCTTTCTGAGGCAGCGGAGAATGGATCTCAGCTAGTGGTGTTCCCGGAGGCTTTCATCGGTGGATATCCACGTGGCTCTACCTTTGAATTGGCTATTGGTTCTCGTACCGCTAAAGGACGAGATGACTTTCGCAAGTACCATGCTTCTGCCATTGATGTTCCTGGCCCTGAAGTGGAACGATTAGCGTTAATGGCCAAGAAGTACAAAGTATACTTGGTTATGGGTGTGATAGAGAGGGAAGGCTACACGCTATACTGCACCGTTCTTTTCTTCGATTCACAAGGTCTGTTCTTAGGTAAGCACCGCAAACTCATGCCTACAGCTCTTGAACGTTGCATTTGGGGATTTGGAGATGGATCAACCATCCCTGTGTTCGATACTCCTATTGGGAAAATCGGTGCTGCTATTTGTTGGGAAAATAGGATGCCTTCTTTGAGAACCGCAATGTATGCCAAAGGCATTGAGATTTATTGTGCACCTACTGCTGATTCAAGAGAAACTTGGCTAGCATCAATGACTCATATTGCACTTGAGGGTGGATGTTTTGTTTTGTCAGCTAACCAGTTTTGTCGTCGGAAAGACTATCCTTCTCCGCCGGAATACATGTTTTCCGGTTCAGAAGAGAGCCTAACACCGGACTCTGTTGTCTGCGCTGGTGGAAGCTCTATCATTTCACCTTTGGGAATTGTTTTAGCTGGACCAAACTATAGAGGAGAAGCTCTTATCACAGCTGATCTAGATCTTGGGGACATAGCACGAGCCAAGTTTGATTTTGATGTGGTCGGACATTACTCGAGGCCTGAAGTGTTTAGCTTGAACATAAGGGAGCATCCGAGAAAAGCGGTCAGCTTCAAGACGTCAAAGGTAATGGAAGATGAATCCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000257 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009636 GO:0009683 GO:0009684 GO:0009850 GO:0009851 GO:0009970 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0010817 GO:0016020 GO:0016053 GO:0016143 GO:0016145 GO:0016787 GO:0016810 GO:0016815 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0018822 GO:0019438 GO:0019499 GO:0019748 GO:0019752 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0030054 GO:0031667 GO:0031668 GO:0031669 GO:0032787 GO:0033554 GO:0034641 GO:0034754 GO:0042221 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044248 GO:0044249 GO:0044271 GO:0044273 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046686 GO:0047427 GO:0047558 GO:0048046 GO:0050896 GO:0051410 GO:0051716 GO:0055044 GO:0065007 GO:0065008 GO:0071496 GO:0071704 GO:0071944 GO:0072330 GO:0080061 GO:0080109 GO:0098754 GO:1901135 GO:1901136 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1901698
KEGG Pathways
Metabolic & Signaling
ko00380 ko00460 ko00627 ko00643 ko00910 ko01100 ko01110 ko01120 map00380 map00460 map00627 map00643 map00910 map01100 map01110 map01120
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

355

Amino Acids

38.89

Weight (kDa)

5.48

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 21 - 222 2.4e-50 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 824
AatII GACGTC 1 cut(s) 1040
AccI GTMKAC 1 cut(s) 390
AciI CCGC 9 cut(s) 35, 84, 153, 194, 288, 484, 621, 773, 1022
AclI AACGTT 1 cut(s) 511
AclWI GGATC 2 cut(s) 211, 544
AcoI YGGCCR 2 cut(s) 52, 372
AcuI CTGAAG 2 cut(s) 369, 1002
AcvI CACGTG 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 1037
AfaI GTAC 4 cut(s) 108, 286, 317, 383
AfiI CCNNNNNNNGG 2 cut(s) 352, 814
AflIII ACRYGT 2 cut(s) 78, 783
AgsI TTSAA 5 cut(s) 266, 509, 672, 997, 1033
AjnI CCWGG 1 cut(s) 340
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 2 cut(s) 27, 169
Alw44I GTGCAC 1 cut(s) 654
AlwI GGATC 2 cut(s) 211, 544
Ama87I CYCGRG 1 cut(s) 973
AoxI GGCC 4 cut(s) 52, 343, 372, 977
ApaLI GTGCAC 1 cut(s) 654
ApeKI GCWGC 2 cut(s) 191, 581
AspLEI GCGC 1 cut(s) 833
AspS9I GGNCC 2 cut(s) 344, 878
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 1 cut(s) 846
AsuNHI GCTAGC 1 cut(s) 685
AvaI CYCGRG 1 cut(s) 973
AvaII GGWCC 1 cut(s) 878
BaeGI GKGCMC 1 cut(s) 658
BalI TGGCCA 1 cut(s) 374
BauI CACGAG 1 cut(s) 936
BbrPI CACGTG 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 2 cut(s) 203, 568
BccI CCATC 4 cut(s) 63, 110, 527, 551
BceAI ACGGC 2 cut(s) 48, 67
BciT130I CCWGG 1 cut(s) 342
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 27, 169
BfaI CTAG 4 cut(s) 161, 212, 686, 917
BfmI CTRYAG 4 cut(s) 114, 132, 498, 886
BglII AGATCT 1 cut(s) 919
BisI GCNGC 3 cut(s) 36, 192, 582
BlsI GCNGC 3 cut(s) 37, 193, 583
Bme1390I CCNGG 2 cut(s) 224, 342
Bme18I GGWCC 1 cut(s) 878
BmeT110I CYCGRG 1 cut(s) 973
BmgT120I GGNCC 2 cut(s) 344, 878
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 224, 342
BmsI GCATC 3 cut(s) 594, 698, 1018
BmtI GCTAGC 1 cut(s) 689
BpiI GAAGAC 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 251
BsaBI GATNNNNATC 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 1037
BsaWI WCCGGW 2 cut(s) 791, 814
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 814
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 742
Bse3DI GCAATG 1 cut(s) 630
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 1 cut(s) 342
BseGI GGATG 4 cut(s) 543, 609, 725, 1009
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 2 cut(s) 352, 814
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 3 cut(s) 60, 177, 221
BseNI ACTGG 1 cut(s) 742
BseRI GAGGAG 2 cut(s) 88, 906
BseSI GKGCMC 1 cut(s) 658
BseXI GCAGC 2 cut(s) 203, 568
BsgI GTGCAG 1 cut(s) 421
BshFI GGCC 4 cut(s) 54, 345, 374, 979
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 1 cut(s) 973
BsiSI CCGG 5 cut(s) 87, 224, 776, 792, 815
BsiWI CGTACG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 941
BslI CCNNNNNNNGG 2 cut(s) 352, 814
BsmAI GTCTC 2 cut(s) 27, 169
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 941
BsnI GGCC 4 cut(s) 54, 345, 374, 979
BsoBI CYCGRG 1 cut(s) 973
Bsp1286I GDGCHC 1 cut(s) 658
Bsp143I GATC 4 cut(s) 203, 536, 913, 919
BspACI CCGC 9 cut(s) 35, 84, 153, 194, 288, 484, 621, 773, 1022
BspANI GGCC 4 cut(s) 54, 345, 374, 979
BspCNI CTCAG 3 cut(s) 61, 178, 220
BspLI GGNNCC 1 cut(s) 39
BspOI GCTAGC 1 cut(s) 689
BspPI GGATC 2 cut(s) 211, 544
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 742
BssAI RCCGGY 1 cut(s) 86
BssMI GATC 4 cut(s) 203, 536, 913, 919
BssNAI GTATAC 1 cut(s) 391
BssNI GRCGYC 1 cut(s) 1037
BssSI CACGAG 1 cut(s) 936
Bst1107I GTATAC 1 cut(s) 391
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 3 cut(s) 118, 136, 442
Bst6I CTCTTC 1 cut(s) 795
BstACI GRCGYC 1 cut(s) 1037
BstAPI GCANNNNNTGC 2 cut(s) 629, 662
BstBAI YACGTR 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 88, 687
BstDEI CTNAG 4 cut(s) 69, 186, 207, 472
BstF5I GGATG 4 cut(s) 543, 609, 725, 1009
BstHHI GCGC 1 cut(s) 833
BstKTI GATC 4 cut(s) 206, 539, 916, 922
BstMAI GTCTC 2 cut(s) 27, 169
BstMBI GATC 4 cut(s) 203, 536, 913, 919
BstMWI GCNNNNNNNGC 3 cut(s) 371, 629, 662
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 2 cut(s) 82, 787
BstSCI CCNGG 2 cut(s) 222, 340
BstSFI CTRYAG 4 cut(s) 114, 132, 498, 886
BstSLI GKGCMC 1 cut(s) 658
BstV1I GCAGC 2 cut(s) 203, 568
BstV2I GAAGAC 1 cut(s) 130
BstX2I RGATCY 2 cut(s) 203, 919
BstYI RGATCY 2 cut(s) 203, 919
BstZ17I GTATAC 1 cut(s) 391
BsuRI GGCC 4 cut(s) 54, 345, 374, 979
BtsCI GGATG 4 cut(s) 543, 609, 725, 1009
Cac8I GCNNGC 2 cut(s) 88, 687
CfoI GCGC 1 cut(s) 833
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 344, 878
Csp6I GTAC 4 cut(s) 107, 285, 316, 382
CviAII CATG 6 cut(s) 7, 28, 79, 320, 493, 784
CviQI GTAC 4 cut(s) 107, 285, 316, 382
DdeI CTNAG 4 cut(s) 69, 186, 207, 472
DpnI GATC 4 cut(s) 205, 538, 915, 921
DpnII GATC 4 cut(s) 203, 536, 913, 919
DrdI GACNNNNNNGTC 1 cut(s) 824
DseDI GACNNNNNNGTC 1 cut(s) 824
EaeI YGGCCR 2 cut(s) 52, 372
Eam1104I CTCTTC 1 cut(s) 795
EarI CTCTTC 1 cut(s) 795
EciI GGCGGA 2 cut(s) 73, 762
Eco147I AGGCCT 1 cut(s) 979
Eco32I GATATC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 878
Eco57I CTGAAG 2 cut(s) 369, 1002
Eco72I CACGTG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 973
EcoRII CCWGG 1 cut(s) 340
EcoRV GATATC 1 cut(s) 245
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 6 cut(s) 10, 31, 82, 323, 496, 787
FalI AAGNNNNNCTT 2 cut(s) 664, 696
FaqI GGGAC 1 cut(s) 941
FatI CATG 6 cut(s) 6, 27, 78, 319, 492, 783
FauI CCCGC 1 cut(s) 160
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 3 cut(s) 36, 192, 582
FokI GGATG 4 cut(s) 530, 616, 732, 996
Fsp4HI GCNGC 3 cut(s) 36, 192, 582
FspBI CTAG 4 cut(s) 161, 212, 686, 917
GlaI GCGC 1 cut(s) 832
GluI GCNGC 3 cut(s) 36, 192, 582
HaeIII GGCC 4 cut(s) 54, 345, 374, 979
HapII CCGG 5 cut(s) 87, 224, 776, 792, 815
HhaI GCGC 1 cut(s) 833
Hin1I GRCGYC 1 cut(s) 1037
Hin1II CATG 6 cut(s) 10, 31, 82, 323, 496, 787
Hin6I GCGC 1 cut(s) 831
HinP1I GCGC 1 cut(s) 831
HinfI GANTC 6 cut(s) 92, 455, 668, 697, 818, 1058
HpaII CCGG 5 cut(s) 87, 224, 776, 792, 815
HphI GGTGA 1 cut(s) 846
Hpy166II GTNNAC 2 cut(s) 391, 656
Hpy188I TCNGA 5 cut(s) 187, 756, 799, 965, 1014
Hpy188III TCNNGA 4 cut(s) 506, 672, 917, 1033
Hpy8I GTNNAC 2 cut(s) 391, 656
Hpy99I CGWCG 1 cut(s) 756
HpyAV CCTTC 3 cut(s) 413, 618, 777
HpyCH4III ACNGT 3 cut(s) 118, 136, 442
HpyCH4IV ACGT 4 cut(s) 20, 250, 511, 1037
HpyCH4V TGCA 5 cut(s) 10, 438, 516, 656, 707
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 629, 662
HpyF3I CTNAG 4 cut(s) 69, 186, 207, 472
HpySE526I ACGT 4 cut(s) 20, 250, 511, 1037
Hsp92I GRCGYC 1 cut(s) 1037
Hsp92II CATG 6 cut(s) 10, 31, 82, 323, 496, 787
HspAI GCGC 1 cut(s) 831
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 4 cut(s) 203, 536, 913, 919
LmnI GCTCC 2 cut(s) 43, 1006
Lsp1109I GCAGC 2 cut(s) 203, 568
LweI GCATC 3 cut(s) 594, 698, 1018
MaeI CTAG 4 cut(s) 161, 212, 686, 917
MaeII ACGT 4 cut(s) 20, 250, 511, 1037
MalI GATC 4 cut(s) 205, 538, 915, 921
MboI GATC 4 cut(s) 203, 536, 913, 919
MboII GAAGA 5 cut(s) 55, 130, 442, 812, 1064
MflI RGATCY 2 cut(s) 203, 919
MhlI GDGCHC 1 cut(s) 658
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 2 cut(s) 266, 864
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 2 cut(s) 691, 812
MmeI TCCRAC 2 cut(s) 734, 943
MnlI CCTC 9 cut(s) 106, 109, 139, 181, 220, 408, 706, 884, 969
Mox20I TGGCCA 1 cut(s) 374
MreI CGCCGGCG 1 cut(s) 86
MroNI GCCGGC 1 cut(s) 86
MscI TGGCCA 1 cut(s) 374
MseI TTAA 1 cut(s) 368
MslI CAYNNNNRTG 1 cut(s) 548
Msp20I TGGCCA 1 cut(s) 374
MspA1I CMGCKG 2 cut(s) 194, 911
MspI CCGG 5 cut(s) 87, 224, 776, 792, 815
MspR9I CCNGG 2 cut(s) 224, 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 3 cut(s) 371, 629, 662
NaeI GCCGGC 1 cut(s) 88
NciI CCSGG 1 cut(s) 224
NdeII GATC 4 cut(s) 203, 536, 913, 919
NgoMIV GCCGGC 1 cut(s) 86
NheI GCTAGC 1 cut(s) 685
NlaIII CATG 6 cut(s) 10, 31, 82, 323, 496, 787
NlaIV GGNNCC 1 cut(s) 39
NspI RCATGY 2 cut(s) 82, 787
PaeR7I CTCGAG 1 cut(s) 973
PceI AGGCCT 1 cut(s) 979
PciI ACATGT 2 cut(s) 78, 783
PdiI GCCGGC 1 cut(s) 88
PfeI GAWTC 4 cut(s) 92, 455, 668, 1058
Pfl23II CGTACG 1 cut(s) 106
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 3 cut(s) 37, 193, 583
PleI GAGTC 2 cut(s) 691, 812
PmaCI CACGTG 1 cut(s) 251
PmlI CACGTG 1 cut(s) 251
PpsI GAGTC 2 cut(s) 691, 812
Ppu21I YACGTR 1 cut(s) 251
PscI ACATGT 2 cut(s) 78, 783
Psp1406I AACGTT 1 cut(s) 511
Psp6I CCWGG 1 cut(s) 340
PspCI CACGTG 1 cut(s) 251
PspGI CCWGG 1 cut(s) 340
PspLI CGTACG 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 344, 878
PspXI VCTCGAGB 1 cut(s) 973
PsuI RGATCY 2 cut(s) 203, 919
PvuII CAGCTG 1 cut(s) 911
RsaI GTAC 4 cut(s) 108, 286, 317, 383
RsaNI GTAC 4 cut(s) 107, 285, 316, 382
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 1 cut(s) 368
SatI GCNGC 3 cut(s) 36, 192, 582
Sau3AI GATC 4 cut(s) 203, 536, 913, 919
Sau96I GGNCC 2 cut(s) 344, 878
SchI GAGTC 2 cut(s) 691, 812
ScrFI CCNGG 2 cut(s) 224, 342
SduI GDGCHC 1 cut(s) 658
SfaNI GCATC 3 cut(s) 594, 698, 1018
SfcI CTRYAG 4 cut(s) 114, 132, 498, 886
Sfr274I CTCGAG 1 cut(s) 973
SgrAI CRCCGGYG 1 cut(s) 86
SinI GGWCC 1 cut(s) 878
SlaI CTCGAG 1 cut(s) 973
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 2 cut(s) 710, 973
SmoI CTYRAG 2 cut(s) 710, 973
Sse9I AATT 2 cut(s) 266, 864
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 9 cut(s) 35, 84, 153, 194, 288, 484, 621, 773, 1022
SspMI CTAG 4 cut(s) 161, 212, 686, 917
StuI AGGCCT 1 cut(s) 979
StyD4I CCNGG 2 cut(s) 222, 340
TaaI ACNGT 3 cut(s) 118, 136, 442
TaiI ACGT 4 cut(s) 23, 253, 514, 1040
TaqI TCGA 3 cut(s) 453, 555, 974
TasI AATT 2 cut(s) 266, 864
TatI WGTACW 1 cut(s) 381
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 4 cut(s) 92, 455, 668, 1058
Tru1I TTAA 1 cut(s) 368
Tru9I TTAA 1 cut(s) 368
TseI GCWGC 2 cut(s) 191, 581
TspDTI ATGAA 1 cut(s) 223
TspGWI ACGGA 1 cut(s) 1051
VneI GTGCAC 1 cut(s) 654
VpaK11BI GGWCC 1 cut(s) 878
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 2 cut(s) 82, 787
XhoI CTCGAG 1 cut(s) 973
XmiI GTMKAC 1 cut(s) 390
XspI CTAG 4 cut(s) 161, 212, 686, 917
ZraI GACGTC 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.