MD03G1150700.v1.1

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007736
Physical Location & Seq
Reverse (-)
16844251 .. 16850801
6551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1150700.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCTCTGGTACCCTCGTCTTCCCCACTGATCGCGGAGGTCGACATGGGAACCGACTACTCCGCCCCCACTGTCCGCGCCACCGTCGTCCAAGCCTCCACCGTCTTCTACGACACCCCTGCCACTCTAGATAAGGCTGAGAGGTTATTGGCTGAAGCAGCTGGATATGGTGCCCAGCTGGTTGTGTTTCCTGAAGCATTTGTGGGTGGTTATCCACGTGGGTCGAATTTTGGCGTTGTCATTGGGAACAGAACAGCTAAGGGTAAAGAAGACTTCAGAAAGTATCATGCTGCTGCCATTGATGTGCCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTACAAGGTATTCCTGGTAATGGGTGTGATAGAGAGAGATGGATATACACTCTATTGCACTGTTCTGTTTTTTGATTCTCAAGGTTGCTACTTGGGAAAGCACCGAAAAATGATGCCCACAGCACTGGAGCGGATCATTTGGGGATTTGGAGATGGATCTACAATTCCTGTGTTTGAGACTCCGATTGGAAAAATAGGTGCTGCCATTTGTTGGGAAAATAAGATGCCACTTCTAAGGACAGCAATGTATGCTAAAGGCATTGAGATATATTGTGCTCCTACGGCTGATTCCAGGGATTTATGGCAAGCATCAATGACTCATATTGCCTTAGAGGGTGGGTGTTTTGTCCTATCAGCCAATCAGTTCTGTCGAAGGAAAGACTACCCCCCTCCTCCAGAATATGCATTTGCAGGAGAGGAACCTGCTCCAGATTCTGTCGTCTGTGCTGGAGGCAGTGTTATTATCTCACCGTCGGGGACTATTCTTGCTGGACCCAATTATGATGGGGAGGCACTCATCTCAGCAGATCTCGATCTTGGAGAAATAGCAAGGGCGAAATTTGACTTTGATGTGGTTGGCCACTATTCACGGCCCGAAGTGCTTAGCTTGATCGTGAGGGACCATCCAGCAACTCCAGTTACTTTCACATCAGCCTCAGCAAAGACCGATCGCGCAGTCTCACATAAGCCATAA

Protein Analysis

347

Amino Acids

37.2

Weight (kDa)

5.46

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 26 - 302 3.1e-65 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 778
Acc65I GGTACC 1 cut(s) 10
AccB1I GGYRCC 2 cut(s) 10, 170
AccB7I CCANNNNNTGG 1 cut(s) 558
AccBSI CCGCTC 1 cut(s) 478
AccI GTMKAC 1 cut(s) 42
AccII CGCG 3 cut(s) 35, 78, 1020
AciI CCGC 4 cut(s) 35, 63, 76, 478
AclWI GGATC 2 cut(s) 488, 511
AcoI YGGCCR 1 cut(s) 925
AcsI RAATTY 2 cut(s) 226, 905
AcuI CTGAAG 4 cut(s) 174, 213, 259, 336
AcvI CACGTG 1 cut(s) 218
AfaI GTAC 2 cut(s) 12, 350
AfiI CCNNNNNNNGG 2 cut(s) 367, 558
AjnI CCWGG 3 cut(s) 307, 360, 638
AluBI AGCT 4 cut(s) 161, 178, 257, 954
AluI AGCT 4 cut(s) 161, 178, 257, 954
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 2 cut(s) 518, 1030
AlwI GGATC 2 cut(s) 488, 511
AlwNI CAGNNNCTG 1 cut(s) 782
AoxI GGCC 2 cut(s) 925, 938
ApeKI GCWGC 5 cut(s) 158, 290, 293, 332, 548
ApoI RAATTY 2 cut(s) 226, 905
Asp718I GGTACC 1 cut(s) 10
AspLEI GCGC 2 cut(s) 80, 1022
AspS9I GGNCC 4 cut(s) 311, 839, 939, 967
AsuHPI GGTGA 1 cut(s) 807
AvaII GGWCC 3 cut(s) 311, 839, 967
BaeGI GKGCMC 1 cut(s) 175
BaeI ACNNNNGTAYC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 927
BanI GGYRCC 2 cut(s) 10, 170
BbrPI CACGTG 1 cut(s) 218
BbsI GAAGAC 3 cut(s) 12, 97, 276
Bbv12I GWGCWC 1 cut(s) 625
BbvCI CCTCAGC 1 cut(s) 1003
BbvI GCAGC 5 cut(s) 170, 277, 280, 344, 535
BccI CCATC 4 cut(s) 380, 494, 845, 978
BceAI ACGGC 2 cut(s) 645, 953
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 3 cut(s) 309, 362, 640
BcoDI GTCTC 2 cut(s) 518, 1030
BfaI CTAG 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 778
BglII AGATCT 1 cut(s) 874
BisI GCNGC 5 cut(s) 159, 291, 294, 333, 549
BlpI GCTNAGC 1 cut(s) 950
BlsI GCNGC 5 cut(s) 160, 292, 295, 334, 550
Bme1390I CCNGG 3 cut(s) 309, 362, 640
Bme18I GGWCC 3 cut(s) 311, 839, 967
BmgT120I GGNCC 4 cut(s) 311, 839, 939, 967
BmiI GGNNCC 6 cut(s) 12, 52, 172, 768, 841, 968
BmrFI CCNGG 3 cut(s) 309, 362, 640
BmsI GCATC 3 cut(s) 450, 561, 665
BpiI GAAGAC 3 cut(s) 12, 97, 276
BpmI CTGGAG 5 cut(s) 494, 726, 759, 816, 966
Bpu10I CCTNAGC 2 cut(s) 258, 1003
Bpu1102I GCTNAGC 1 cut(s) 950
BpuEI CTTGAG 1 cut(s) 411
Bsa29I ATCGAT 1 cut(s) 325
BsaAI YACGTR 1 cut(s) 218
BsaBI GATNNNNATC 1 cut(s) 879
BsaJI CCNNGG 1 cut(s) 639
BsaXI ACNNNNNCTCC 2 cut(s) 789, 819
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 558
Bse1I ACTGG 2 cut(s) 477, 983
Bse3DI GCAATG 2 cut(s) 342, 597
Bse8I GATNNNNATC 1 cut(s) 879
BseBI CCWGG 3 cut(s) 309, 362, 640
BseCI ATCGAT 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 639
BseGI GGATG 1 cut(s) 970
BseJI GATNNNNATC 1 cut(s) 879
BseLI CCNNNNNNNGG 2 cut(s) 367, 558
BseMI GCAATG 2 cut(s) 342, 597
BseMII CTCAG 3 cut(s) 129, 882, 1017
BseNI ACTGG 2 cut(s) 477, 983
BseRI GAGGAG 1 cut(s) 729
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 5 cut(s) 170, 277, 280, 344, 535
BseYI CCCAGC 1 cut(s) 174
Bsh1236I CGCG 3 cut(s) 35, 78, 1020
Bsh1285I CGRYCG 1 cut(s) 1018
BshFI GGCC 2 cut(s) 927, 940
BshNI GGYRCC 2 cut(s) 10, 170
BshVI ATCGAT 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 625
BslFI GGGAC 2 cut(s) 838, 980
BslI CCNNNNNNNGG 2 cut(s) 367, 558
BsmAI GTCTC 2 cut(s) 518, 1030
BsmFI GGGAC 2 cut(s) 838, 980
BsnI GGCC 2 cut(s) 927, 940
Bsp1286I GDGCHC 2 cut(s) 175, 625
Bsp143I GATC 8 cut(s) 30, 322, 480, 503, 874, 880, 957, 1015
Bsp1720I GCTNAGC 1 cut(s) 950
BspACI CCGC 4 cut(s) 35, 63, 76, 478
BspANI GGCC 2 cut(s) 927, 940
BspCNI CTCAG 3 cut(s) 130, 881, 1016
BspDI ATCGAT 1 cut(s) 325
BspFNI CGCG 3 cut(s) 35, 78, 1020
BspLI GGNNCC 6 cut(s) 12, 52, 172, 768, 841, 968
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 2 cut(s) 488, 511
BspT107I GGYRCC 2 cut(s) 10, 170
BsrBI CCGCTC 1 cut(s) 478
BsrDI GCAATG 2 cut(s) 342, 597
BsrI ACTGG 2 cut(s) 477, 983
BssECI CCNNGG 1 cut(s) 639
BssMI GATC 8 cut(s) 30, 322, 480, 503, 874, 880, 957, 1015
Bst2UI CCWGG 3 cut(s) 309, 362, 640
Bst4CI ACNGT 5 cut(s) 73, 85, 103, 409, 819
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 7 cut(s) 138, 258, 581, 676, 868, 950, 1003
BstF5I GGATG 1 cut(s) 970
BstFNI CGCG 3 cut(s) 35, 78, 1020
BstHHI GCGC 2 cut(s) 80, 1022
BstKTI GATC 8 cut(s) 33, 325, 483, 506, 877, 883, 960, 1018
BstMAI GTCTC 2 cut(s) 518, 1030
BstMBI GATC 8 cut(s) 30, 322, 480, 503, 874, 880, 957, 1015
BstMCI CGRYCG 1 cut(s) 1018
BstMWI GCNNNNNNNGC 5 cut(s) 158, 338, 596, 629, 946
BstNI CCWGG 3 cut(s) 309, 362, 640
BstSCI CCNGG 3 cut(s) 307, 360, 638
BstSLI GKGCMC 1 cut(s) 175
BstUI CGCG 3 cut(s) 35, 78, 1020
BstV1I GCAGC 5 cut(s) 170, 277, 280, 344, 535
BstV2I GAAGAC 3 cut(s) 12, 97, 276
BstX2I RGATCY 2 cut(s) 503, 874
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 2 cut(s) 503, 874
Bsu15I ATCGAT 1 cut(s) 325
BsuRI GGCC 2 cut(s) 927, 940
BsuTUI ATCGAT 1 cut(s) 325
BtsCI GGATG 1 cut(s) 970
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 5 cut(s) 26, 69, 405, 470, 808
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 1 cut(s) 654
CaiI CAGNNNCTG 1 cut(s) 782
CfoI GCGC 2 cut(s) 80, 1022
Cfr13I GGNCC 4 cut(s) 311, 839, 939, 967
ClaI ATCGAT 1 cut(s) 325
Csp6I GTAC 2 cut(s) 11, 349
CviAII CATG 2 cut(s) 46, 287
CviQI GTAC 2 cut(s) 11, 349
DdeI CTNAG 7 cut(s) 138, 258, 581, 676, 868, 950, 1003
DpnI GATC 8 cut(s) 32, 324, 482, 505, 876, 882, 959, 1017
DpnII GATC 8 cut(s) 30, 322, 480, 503, 874, 880, 957, 1015
EaeI YGGCCR 1 cut(s) 925
EciI GGCGGA 1 cut(s) 52
Eco47I GGWCC 3 cut(s) 311, 839, 967
Eco57I CTGAAG 4 cut(s) 174, 213, 259, 336
Eco72I CACGTG 1 cut(s) 218
EcoRII CCWGG 3 cut(s) 307, 360, 638
EcoT22I ATGCAT 1 cut(s) 754
FaeI CATG 2 cut(s) 49, 290
FaqI GGGAC 2 cut(s) 838, 980
FatI CATG 2 cut(s) 45, 286
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 5 cut(s) 159, 291, 294, 333, 549
FokI GGATG 1 cut(s) 957
Fsp4HI GCNGC 5 cut(s) 159, 291, 294, 333, 549
FspBI CTAG 1 cut(s) 128
GlaI GCGC 2 cut(s) 79, 1021
GluI GCNGC 5 cut(s) 159, 291, 294, 333, 549
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 5 cut(s) 494, 726, 759, 816, 966
HaeIII GGCC 2 cut(s) 927, 940
HhaI GCGC 2 cut(s) 80, 1022
Hin1II CATG 2 cut(s) 49, 290
Hin6I GCGC 2 cut(s) 78, 1020
HinP1I GCGC 2 cut(s) 78, 1020
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 5 cut(s) 422, 526, 635, 664, 779
HphI GGTGA 1 cut(s) 807
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 278, 531
Hpy188III TCNNGA 7 cut(s) 128, 191, 314, 743, 776, 878, 961
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 2 cut(s) 89, 823
HpyAV CCTTC 1 cut(s) 714
HpyCH4III ACNGT 5 cut(s) 73, 85, 103, 409, 819
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 3 cut(s) 405, 752, 758
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 338, 596, 629, 946
HpyF3I CTNAG 7 cut(s) 138, 258, 581, 676, 868, 950, 1003
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 2 cut(s) 49, 290
HspAI GCGC 2 cut(s) 78, 1020
KpnI GGTACC 1 cut(s) 14
Kzo9I GATC 8 cut(s) 30, 322, 480, 503, 874, 880, 957, 1015
LmnI GCTCC 3 cut(s) 475, 628, 778
Lsp1109I GCAGC 5 cut(s) 170, 277, 280, 344, 535
LweI GCATC 3 cut(s) 450, 561, 665
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 1 cut(s) 985
MalI GATC 8 cut(s) 32, 324, 482, 505, 876, 882, 959, 1017
MbiI CCGCTC 1 cut(s) 478
MboI GATC 8 cut(s) 30, 322, 480, 503, 874, 880, 957, 1015
MboII GAAGA 3 cut(s) 12, 97, 281
MflI RGATCY 2 cut(s) 503, 874
MhlI GDGCHC 2 cut(s) 175, 625
MlsI TGGCCA 1 cut(s) 927
MluCI AATT 4 cut(s) 226, 510, 844, 905
MluNI TGGCCA 1 cut(s) 927
MlyI GAGTC 2 cut(s) 520, 658
Mox20I TGGCCA 1 cut(s) 927
Mph1103I ATGCAT 1 cut(s) 754
MscI TGGCCA 1 cut(s) 927
MslI CAYNNNNRTG 1 cut(s) 302
Msp20I TGGCCA 1 cut(s) 927
MspA1I CMGCKG 2 cut(s) 161, 178
MspR9I CCNGG 3 cut(s) 309, 362, 640
MvaI CCWGG 3 cut(s) 309, 362, 640
MvnI CGCG 3 cut(s) 35, 78, 1020
MwoI GCNNNNNNNGC 5 cut(s) 158, 338, 596, 629, 946
NdeII GATC 8 cut(s) 30, 322, 480, 503, 874, 880, 957, 1015
NlaIII CATG 2 cut(s) 49, 290
NlaIV GGNNCC 6 cut(s) 12, 52, 172, 768, 841, 968
NsiI ATGCAT 1 cut(s) 754
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 3 cut(s) 422, 635, 779
PflMI CCANNNNNTGG 1 cut(s) 558
PkrI GCNGC 5 cut(s) 160, 292, 295, 334, 550
Ple19I CGATCG 1 cut(s) 1018
PleI GAGTC 2 cut(s) 520, 658
PmaCI CACGTG 1 cut(s) 218
PmlI CACGTG 1 cut(s) 218
PpsI GAGTC 2 cut(s) 520, 658
Ppu21I YACGTR 1 cut(s) 218
Psp6I CCWGG 3 cut(s) 307, 360, 638
PspCI CACGTG 1 cut(s) 218
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 3 cut(s) 307, 360, 638
PspN4I GGNNCC 6 cut(s) 12, 52, 172, 768, 841, 968
PspPI GGNCC 4 cut(s) 311, 839, 939, 967
PstNI CAGNNNCTG 1 cut(s) 782
PsuI RGATCY 2 cut(s) 503, 874
PvuI CGATCG 1 cut(s) 1018
PvuII CAGCTG 2 cut(s) 161, 178
RsaI GTAC 2 cut(s) 12, 350
RsaNI GTAC 2 cut(s) 11, 349
RseI CAYNNNNRTG 1 cut(s) 302
SalI GTCGAC 1 cut(s) 41
SatI GCNGC 5 cut(s) 159, 291, 294, 333, 549
Sau3AI GATC 8 cut(s) 30, 322, 480, 503, 874, 880, 957, 1015
Sau96I GGNCC 4 cut(s) 311, 839, 939, 967
SchI GAGTC 2 cut(s) 520, 658
ScrFI CCNGG 3 cut(s) 309, 362, 640
SduI GDGCHC 2 cut(s) 175, 625
SfaNI GCATC 3 cut(s) 450, 561, 665
SinI GGWCC 3 cut(s) 311, 839, 967
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 4 cut(s) 226, 510, 844, 905
SsiI CCGC 4 cut(s) 35, 63, 76, 478
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 3 cut(s) 307, 360, 638
TaaI ACNGT 5 cut(s) 73, 85, 103, 409, 819
TaiI ACGT 1 cut(s) 220
TaqI TCGA 5 cut(s) 42, 224, 325, 718, 879
TaqII GACCGA 1 cut(s) 1028
TasI AATT 4 cut(s) 226, 510, 844, 905
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 3 cut(s) 422, 635, 779
TscAI CASTG 5 cut(s) 33, 76, 412, 477, 808
TseI GCWGC 5 cut(s) 158, 290, 293, 332, 548
TspRI CASTG 5 cut(s) 33, 76, 412, 477, 808
Van91I CCANNNNNTGG 1 cut(s) 558
VpaK11BI GGWCC 3 cut(s) 311, 839, 967
XapI RAATTY 2 cut(s) 226, 905
XbaI TCTAGA 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.