Rh5AG312500

Bifunctional nitrilase nitrile hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007736
Physical Location & Seq
Reverse (-)
45167150 .. 45173852
6703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG312500.1

Sequence Viewer

Length: 1110 bp
ATGCAATGTGCGAAAGTAAAGACAAAGAGATTCCAGCCTCCAAATTCGAATACAAAAACTGATTGGTGTGAAACTGAGAGAGACCAAATGGCTCTGGTACCCTCATCTGCGCCAGTGAACGAGAGCCCAGTGATCACCGAGGTTGACATGGGCGGCGACGACTCCACACCCACCGTCCGAGCCACCGTCGTCCAAGCCTCCACCGTCTTCTACGACACTCCCGCCACTCTAGATAAGGCTGAGAGATTGTTGGCTGAAGCAGCTGGGTATGGATCGCAGCTGGTTGTGTTTCCGGAAGCATTTGTGGGAGGGTATCCGCGGGGCTCCAATTTCGGGGTTGCGATTGGGAACAGAACTGCCAAGGGGAAAGAAGAGTTCCGAAAGTATCATGCCGCAGCCATTACTGTGCCTGGTCCTGAAGTTGACCGATTGGCATCAATGGCTGGAAAATATAAGGTATTCTTGGTAATGGGTGTGATAGAGAAAGAAGGGTATACTCTTTATTGTTCTGTTCTGTTTTTTGATTCTCAAGGTTGCTACCTGGGAAAGCACCGAAAAATAATGCCAACAGCATTAGAGCGAATCATTTGGGGATTTGGAGATGGATCCACAATTCCTGTGTTTGATACTCCAATTGGAAAAATAGGTGCTGCCATTTGTTGGGAAAATAAGATGCCACTTTTAAGGACAGCAATGTATGCTAAAGGCATTGAAATATATTGTGCTCCTACAGCTGATTCTAGGGATCTATGGCAAGCGTCAATGACCCATATTGCTTTGGAAGGTGGGTGCTTTGTCTTATCAGCTAACCAATTCTGTCGAAGGAAAGACTATCCCCCTCCACCAGAGTATATATTTTCAGGCACGGAAGAGGAACTTACTCCTGATTCTGTTGTCTGTGCTGGAGGCAGTGTTATCATATCACCATCAGGAACTATACTTGCTGGGCCCAATTATGACGGGGAGGCACTCATCTCAGCAGATCTTGGTATGCCATGTTTTTTATGGTACTCTCCTATTGTTAATCAGTTTTCTGTGTTTGAAGAAATTAAAATAATTAAGAGTTTCTGTATTACAAATTGGGTCATTATTTATTTTATTTTGCTGTAA

Protein Analysis

369

Amino Acids

40.4

Weight (kDa)

5.22

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 60 - 329 6.1e-62 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 97
AccB7I CCANNNNNTGG 1 cut(s) 660
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 319
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 5 cut(s) 153, 222, 317, 319, 393
AclWI GGATC 4 cut(s) 280, 600, 613, 753
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 2 cut(s) 276, 438
AfaI GTAC 2 cut(s) 99, 1010
AfiI CCNNNNNNNGG 2 cut(s) 333, 660
AgsI TTSAA 2 cut(s) 713, 1043
AjnI CCWGG 2 cut(s) 409, 540
AluBI AGCT 4 cut(s) 263, 280, 734, 806
AluI AGCT 4 cut(s) 263, 280, 734, 806
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 4 cut(s) 280, 600, 613, 753
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 1 cut(s) 947
ApaI GGGCCC 1 cut(s) 951
ApeKI GCWGC 4 cut(s) 260, 277, 395, 650
ApoI RAATTY 1 cut(s) 43
Asp718I GGTACC 1 cut(s) 97
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 3 cut(s) 413, 947, 948
AsuHPI GGTGA 2 cut(s) 127, 915
AsuII TTCGAA 1 cut(s) 47
AvaII GGWCC 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 951
BamHI GGATCC 1 cut(s) 605
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 3 cut(s) 128, 326, 951
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 4 cut(s) 272, 289, 407, 637
BccI CCATC 2 cut(s) 596, 934
BciT130I CCWGG 2 cut(s) 411, 542
BciVI GTATCC 1 cut(s) 324
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 2 cut(s) 230, 741
BfmI CTRYAG 1 cut(s) 729
BfuI GTATCC 1 cut(s) 324
BglII AGATCT 1 cut(s) 982
BisI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
BlsI GCNGC 6 cut(s) 155, 262, 279, 394, 397, 652
Bme1390I CCNGG 2 cut(s) 411, 542
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 3 cut(s) 413, 947, 948
BmiI GGNNCC 4 cut(s) 99, 325, 607, 949
BmrFI CCNGG 2 cut(s) 411, 542
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 2 cut(s) 443, 663
BmuI ACTGGG 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 924
Bpu14I TTCGAA 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 513
BsaBI GATNNNNATC 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 4 cut(s) 138, 317, 360, 541
BsaWI WCCGGW 1 cut(s) 292
BsaXI ACNNNNNCTCC 2 cut(s) 897, 927
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 660
Bse1I ACTGG 2 cut(s) 113, 128
Bse3DI GCAATG 2 cut(s) 11, 699
Bse8I GATNNNNATC 1 cut(s) 433
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 2 cut(s) 411, 542
BseDI CCNNGG 4 cut(s) 138, 317, 360, 541
BseJI GATNNNNATC 1 cut(s) 433
BseLI CCNNNNNNNGG 2 cut(s) 333, 660
BseMI GCAATG 2 cut(s) 11, 699
BseMII CTCAG 3 cut(s) 66, 231, 990
BseNI ACTGG 2 cut(s) 113, 128
BseSI GKGCMC 1 cut(s) 951
BseXI GCAGC 4 cut(s) 272, 289, 407, 637
BseYI CCCAGC 2 cut(s) 263, 944
Bsh1236I CGCG 1 cut(s) 319
BshFI GGCC 1 cut(s) 949
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 727
BsiSI CCGG 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 333, 660
BsmAI GTCTC 1 cut(s) 75
BsnI GGCC 1 cut(s) 949
Bso31I GGTCTC 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 47
Bsp120I GGGCCC 1 cut(s) 947
Bsp1286I GDGCHC 4 cut(s) 128, 326, 727, 951
Bsp13I TCCGGA 1 cut(s) 292
Bsp143I GATC 5 cut(s) 132, 272, 605, 745, 982
BspACI CCGC 5 cut(s) 153, 222, 317, 319, 393
BspANI GGCC 1 cut(s) 949
BspCNI CTCAG 3 cut(s) 67, 232, 989
BspEI TCCGGA 1 cut(s) 292
BspFNI CGCG 1 cut(s) 319
BspLI GGNNCC 4 cut(s) 99, 325, 607, 949
BspPI GGATC 4 cut(s) 280, 600, 613, 753
BspT104I TTCGAA 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 11, 699
BsrI ACTGG 2 cut(s) 113, 128
BssECI CCNNGG 4 cut(s) 138, 317, 360, 541
BssMI GATC 5 cut(s) 132, 272, 605, 745, 982
BssNAI GTATAC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 360
Bst1107I GTATAC 1 cut(s) 495
Bst2UI CCWGG 2 cut(s) 411, 542
Bst4CI ACNGT 4 cut(s) 175, 187, 205, 406
Bst6I CTCTTC 2 cut(s) 366, 864
BstAPI GCANNNNNTGC 1 cut(s) 698
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 756
BstDEI CTNAG 3 cut(s) 75, 240, 976
BstDSI CCRYGG 1 cut(s) 317
BstFNI CGCG 1 cut(s) 319
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 5 cut(s) 135, 275, 608, 748, 985
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 5 cut(s) 132, 272, 605, 745, 982
BstMWI GCNNNNNNNGC 4 cut(s) 260, 440, 698, 731
BstNI CCWGG 2 cut(s) 411, 542
BstSCI CCNGG 2 cut(s) 409, 540
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 1 cut(s) 951
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 4 cut(s) 272, 289, 407, 637
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 3 cut(s) 605, 745, 982
BstYI RGATCY 3 cut(s) 605, 745, 982
BstZ17I GTATAC 1 cut(s) 495
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 1 cut(s) 949
BtgI CCRYGG 1 cut(s) 317
BtsI GCAGTG 1 cut(s) 916
BtsIMutI CAGTG 3 cut(s) 120, 135, 916
Cac8I GCNNGC 1 cut(s) 756
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 3 cut(s) 413, 947, 948
Cfr42I CCGCGG 1 cut(s) 320
CseI GACGC 1 cut(s) 747
Csp6I GTAC 2 cut(s) 98, 1009
CviAII CATG 3 cut(s) 148, 389, 996
CviQI GTAC 2 cut(s) 98, 1009
DdeI CTNAG 3 cut(s) 75, 240, 976
DpnI GATC 5 cut(s) 134, 274, 607, 747, 984
DpnII GATC 5 cut(s) 132, 272, 605, 745, 982
Eam1104I CTCTTC 2 cut(s) 366, 864
EarI CTCTTC 2 cut(s) 366, 864
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 3 cut(s) 128, 326, 951
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 276, 438
EcoRII CCWGG 2 cut(s) 409, 540
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 3 cut(s) 128, 326, 951
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 3 cut(s) 151, 392, 999
FalI AAGNNNNNCTT 4 cut(s) 446, 478, 861, 893
FatI CATG 3 cut(s) 147, 388, 995
FauI CCCGC 2 cut(s) 229, 312
FbaI TGATCA 1 cut(s) 132
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
FriOI GRGCYC 3 cut(s) 128, 326, 951
Fsp4HI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
FspBI CTAG 2 cut(s) 230, 741
GlaI GCGC 1 cut(s) 111
GluI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
GsaI CCCAGC 2 cut(s) 267, 948
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 1 cut(s) 949
HapII CCGG 1 cut(s) 293
HgaI GACGC 1 cut(s) 747
HhaI GCGC 1 cut(s) 112
Hin1II CATG 3 cut(s) 151, 392, 999
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 2 cut(s) 145, 424
HindII GTYRAC 2 cut(s) 145, 424
HinfI GANTC 6 cut(s) 30, 161, 524, 582, 737, 887
HpaII CCGG 1 cut(s) 293
HphI GGTGA 2 cut(s) 127, 915
Hpy166II GTNNAC 4 cut(s) 118, 145, 424, 495
Hpy188I TCNGA 2 cut(s) 179, 380
Hpy188III TCNNGA 5 cut(s) 230, 293, 416, 884, 930
Hpy8I GTNNAC 4 cut(s) 118, 145, 424, 495
Hpy99I CGWCG 2 cut(s) 161, 191
HpyAV CCTTC 3 cut(s) 482, 776, 816
HpyCH4III ACNGT 4 cut(s) 175, 187, 205, 406
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 440, 698, 731
HpyF3I CTNAG 3 cut(s) 75, 240, 976
Hsp92II CATG 3 cut(s) 151, 392, 999
HspAI GCGC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 292
KpnI GGTACC 1 cut(s) 101
Ksp22I TGATCA 1 cut(s) 132
KspI CCGCGG 1 cut(s) 320
Kzo9I GATC 5 cut(s) 132, 272, 605, 745, 982
LmnI GCTCC 2 cut(s) 329, 730
Lsp1109I GCAGC 4 cut(s) 272, 289, 407, 637
LweI GCATC 2 cut(s) 443, 663
MaeI CTAG 2 cut(s) 230, 741
MalI GATC 5 cut(s) 134, 274, 607, 747, 984
MboI GATC 5 cut(s) 132, 272, 605, 745, 982
MboII GAAGA 4 cut(s) 199, 383, 881, 1055
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 3 cut(s) 605, 745, 982
MhlI GDGCHC 4 cut(s) 128, 326, 727, 951
MluCI AATT 9 cut(s) 43, 328, 612, 633, 812, 952, 1047, 1056, 1078
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 9 cut(s) 48, 112, 133, 208, 302, 849, 865, 899, 958
MroI TCCGGA 1 cut(s) 292
MseI TTAA 4 cut(s) 683, 1023, 1050, 1059
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 4 cut(s) 263, 280, 319, 734
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 2 cut(s) 411, 542
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 2 cut(s) 411, 542
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 4 cut(s) 260, 440, 698, 731
NdeII GATC 5 cut(s) 132, 272, 605, 745, 982
NlaIII CATG 3 cut(s) 151, 392, 999
NlaIV GGNNCC 4 cut(s) 99, 325, 607, 949
NspV TTCGAA 1 cut(s) 47
PfeI GAWTC 5 cut(s) 30, 524, 582, 737, 887
PflMI CCANNNNNTGG 1 cut(s) 660
PkrI GCNGC 6 cut(s) 155, 262, 279, 394, 397, 652
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Psp6I CCWGG 2 cut(s) 409, 540
PspFI CCCAGC 2 cut(s) 263, 944
PspGI CCWGG 2 cut(s) 409, 540
PspN4I GGNNCC 4 cut(s) 99, 325, 607, 949
PspOMI GGGCCC 1 cut(s) 947
PspPI GGNCC 3 cut(s) 413, 947, 948
PsuI RGATCY 3 cut(s) 605, 745, 982
PvuII CAGCTG 3 cut(s) 263, 280, 734
RsaI GTAC 2 cut(s) 99, 1010
RsaNI GTAC 2 cut(s) 98, 1009
RseI CAYNNNNRTG 1 cut(s) 404
SacII CCGCGG 1 cut(s) 320
SaqAI TTAA 4 cut(s) 683, 1023, 1050, 1059
SatI GCNGC 6 cut(s) 154, 261, 278, 393, 396, 651
Sau3AI GATC 5 cut(s) 132, 272, 605, 745, 982
Sau96I GGNCC 3 cut(s) 413, 947, 948
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 411, 542
SduI GDGCHC 4 cut(s) 128, 326, 727, 951
SfaNI GCATC 2 cut(s) 443, 663
SfcI CTRYAG 1 cut(s) 729
Sfr303I CCGCGG 1 cut(s) 320
SfuI TTCGAA 1 cut(s) 47
SgrBI CCGCGG 1 cut(s) 320
SinI GGWCC 1 cut(s) 413
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
Sse9I AATT 9 cut(s) 43, 328, 612, 633, 812, 952, 1047, 1056, 1078
SsiI CCGC 5 cut(s) 153, 222, 317, 319, 393
SspMI CTAG 2 cut(s) 230, 741
StyD4I CCNGG 2 cut(s) 409, 540
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 4 cut(s) 175, 187, 205, 406
TaqI TCGA 2 cut(s) 47, 820
TaqII GACCGA 1 cut(s) 441
TasI AATT 9 cut(s) 43, 328, 612, 633, 812, 952, 1047, 1056, 1078
TauI GCSGC 2 cut(s) 156, 395
TfiI GAWTC 5 cut(s) 30, 524, 582, 737, 887
Tru1I TTAA 4 cut(s) 683, 1023, 1050, 1059
Tru9I TTAA 4 cut(s) 683, 1023, 1050, 1059
TscAI CASTG 3 cut(s) 120, 135, 916
TseI GCWGC 4 cut(s) 260, 277, 395, 650
TspGWI ACGGA 1 cut(s) 881
TspRI CASTG 3 cut(s) 120, 135, 916
Van91I CCANNNNNTGG 1 cut(s) 660
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 1002
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 2 cut(s) 230, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.